Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,165,4440AC23.2% 1046.1 / 77.5 444T71P (ACG→CCG) yfiFmethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (58/280);  new base C (102/0);  total (164/280)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 449 total aligned reads displayed.

ATGATTCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACTTTTCC  >  DH1/1165295‑1165594
                                                                                                                                                     |                                                                                                                                                        
      cTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGGTTc                                                                                                                                                  >  8:685094/1‑151 (MQ=255)
         aTAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCcg                                                                                                                                               <  4:411766/151‑1 (MQ=255)
           aaaCGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGGC‑CGGGTTGCcgcg                                                                                                                                             >  2:408642/1‑151 (MQ=255)
           aaaCGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGTTTcccccc                                                                                                                                             >  4:865217/1‑148 (MQ=255)
           aaaCGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCcgcg                                                                                                                                             <  7:1697703/151‑1 (MQ=255)
             aCGTACCCAGAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGAAGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCcgcgcg                                                                                                                                           <  2:129122/151‑1 (MQ=255)
              cGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGGCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCgcgcgc                                                                                                                                          <  4:2102021/151‑1 (MQ=255)
                   ccACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGt                                                                                                                                     <  4:1901641/151‑1 (MQ=255)
                   ccACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGt                                                                                                                                     <  7:718877/151‑1 (MQ=255)
                    cacaACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGGTTCCCGCGCGCCcgggg                                                                                                                                    >  3:1441172/1‑149 (MQ=255)
                    cacaACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGtg                                                                                                                                    <  5:15840/151‑1 (MQ=255)
                     acaACCGGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGtga                                                                                                                                   <  5:2140915/151‑1 (MQ=255)
                     acaACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTGCCCCCCGCCgggggg                                                                                                                                   >  1:101294/1‑148 (MQ=255)
                     acaACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGtga                                                                                                                                   <  1:1602959/151‑1 (MQ=255)
                         cccGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGGGC‑CGGGGTCCCGCGGGCCgcgggggggg                                                                                                                               >  6:1967685/1‑142 (MQ=255)
                           cgcgTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCCCC‑CCGTTTCCCCCCCGCCGGGggatgcgcc                                                                                                                             >  3:1319413/1‑147 (MQ=255)
                             cgTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGGTTGCCGCGCGCCGGGGGATGAGACGc                                                                                                                           >  5:349902/1‑151 (MQ=255)
                              gTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGGTTACCCCGCGCCcgggggggccccgcc                                                                                                                          >  3:587153/1‑139 (MQ=255)
                                aCCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGGGAGCGGTTGCCGGCGCCCgggggggggggcgccgg                                                                                                                         >  8:1570565/1‑138 (MQ=255)
                                aCCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCgg                                                                                                                        <  6:1158811/151‑1 (MQ=255)
                                  cGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑aa                                                                                                                     <  4:1769619/151‑1 (MQ=255)
                                     gAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAg                                                                                                                  <  6:1764499/151‑1 (MQ=255)
                                     gAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTAGCCGTCGCCCCGCCCGCGATGACAAACAGACTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAg                                                                                                                  <  2:152731/151‑1 (MQ=255)
                                      aaaGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAgg                                                                                                                 <  1:1175321/151‑1 (MQ=255)
                                         gggggCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCg                                                                                                              <  1:1738397/151‑1 (MQ=255)
                                         gggggCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCg                                                                                                              <  1:2263045/151‑1 (MQ=255)
                                             gcgggcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCa                                                                                                          <  4:2247455/151‑1 (MQ=255)
                                                ggcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGGCC‑CCGGTTGCCGCCCGCCCGGGGGTGAGACCCCGGCAAAAGGcccagacgg                                                                                                        >  6:476533/1‑144 (MQ=255)
                                                     ccAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCa                                                                                                  <  8:2072883/151‑1 (MQ=255)
                                                     ccAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CCGTTTACCCCGCGCCCGGTGATGAGACGCCGG‑CAAAGGCCCCTCACGgggggc                                                                                                  >  8:1720104/1‑149 (MQ=255)
                                                      cAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGGC‑CGGGTTGCCGCGCGCCCGGGGGTGCGACGCCCG‑AAAACGCCgcgccccgggggcg                                                                                                 >  4:773976/1‑138 (MQ=255)
                                                       aGGAAAATCTCGTTCTGACGGTGGCCGTCGCCACGCCCGCGATGACAAACAGAGTAAGCCCCGTGACCGCAAGTGGGAAGATTCGCCCTCGCGCC‑CGGTTTACAGGGCGAACGGGGATGAGACGGCGG‑AAAAAGACGCTAACGGTGGCaa                                                                                                 >  6:371368/1‑149 (MQ=255)
                                                       aGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATc                                                                                                <  3:532294/151‑1 (MQ=255)
                                                          aaaaTCTCGTGCTTACGGTGGCCGTCGCCCAGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGATACGCCGG‑AAAAGGCCGATCACGGTGGCATCAg                                                                                              <  6:983644/150‑1 (MQ=255)
                                                           aaaTCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTg                                                                                            <  6:792406/151‑1 (MQ=255)
                                                           aaaTCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTg                                                                                            <  4:644987/151‑1 (MQ=255)
                                                               ctcGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGGTTGCCGCGCGCCGGGGGGTGAGACGCCCG‑CAAAGGCCGCTCCCGGTGGGCTCAgggggt                                                                                         >  1:1390195/1‑148 (MQ=255)
                                                                 cGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGt                                                                                          <  4:1666163/147‑1 (MQ=255)
                                                                 cGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTa                                                                                         <  4:1003623/148‑1 (MQ=255)
                                                                       gACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCCCA‑CCGGTTGCCGCGCGCCGGGGGGTGTGACGCCGG‑CAAAGGCCGCTCACCGGGGGCTCACTGGTAAAAGgtttt                                                                                >  4:1979466/1‑150 (MQ=255)
                                                                        aCGGTGGCCTTAGCCCCGCCCGAGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CTGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTTGTAAAAGTTTTAt                                                                               <  6:1473685/151‑1 (MQ=255)
                                                                        aCGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTAt                                                                               <  4:554705/151‑1 (MQ=255)
                                                                         cGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGGGC‑CGGGTTGGCGCGCGGCGGGGGCTGGGACGCCGG‑GCAAGGGCGCTCGCGGGGGGGTGAGGGGTTAAAGTtttttt                                                                              >  8:1310579/1‑151 (MQ=255)
                                                                         cGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTAtt                                                                              <  5:1816551/151‑1 (MQ=255)
                                                                          ggTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CCGGTTACCGCGCGCCCGGGGCTGAGACCCCCG‑CAAACGCCGCTCACCGGGGCAGGAGGGGTGAAAATttttttt                                                                             >  6:1253484/1‑150 (MQ=255)
                                                                          ggTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTg                                                                             <  8:22810/151‑1 (MQ=255)
                                                                           gTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGa                                                                            <  3:774063/151‑1 (MQ=255)
                                                                            gggCCGGCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGAt                                                                           <  8:287611/150‑1 (MQ=255)
                                                                               ccGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCg                                                                        <  2:920372/151‑1 (MQ=255)
                                                                               ccGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCg                                                                        <  3:396612/151‑1 (MQ=255)
                                                                                   cgccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGaa                                                                     <  2:1629349/150‑1 (MQ=255)
                                                                                   cgccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTACCGCGCGCCGGGGGGTGAGCCGCCGG‑AAAAGGCCGCTCACGGTGGCCTCAGTGGTAAAATTTTTTTTGCTCCggc                                                                      >  3:178170/1‑148 (MQ=255)
                                                                                    gccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGGCC‑CCGGTTGCCGCGCGCCGGGTGGTGAGCCGCCCG‑CAAAGGCCGCGCACGGGGGGATCAGTGGTTAAACTTTTTTTTATCCGGaatt                                                                   >  5:1128128/1‑149 (MQ=255)
                                                                                    gccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CCGTTTCCCCCGCGCCGGGGGGTGAGCCGCCGG‑GAAAGGCCGCTCCCGGGGGCCTCAGTGGTAAAAGTTTTATTTATCCCGAAgt                                                                   >  1:514053/1‑151 (MQ=255)
                                                                                    gccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAgt                                                                   <  4:1317331/151‑1 (MQ=255)
                                                                                      cccgcccgCGATGACAAACAGAGTCAGCCGCGTGACCGCAAGTGGGAAGATTCGCCGTCGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGGTTTATTGATCCGGAAgtgt                                                                 <  7:1306505/151‑1 (MQ=255)
                                                                                      cccgcccgCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAgtgt                                                                 <  3:1590804/151‑1 (MQ=255)
                                                                                       ccgcccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGGTTCCCGCGCGCCGGGGGGTGAGGCGCCGG‑GAAAGGCCGCGCCCCGGGGCATCAGTGGTCAAAGTTTTATTGAGACGGTCGTGtt                                                                >  5:756861/1‑151 (MQ=255)
                                                                                        cgcccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTg                                                               <  2:222924/151‑1 (MQ=255)
                                                                                          cccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGcg                                                             <  8:143139/151‑1 (MQ=255)
                                                                                          cccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGcg                                                             <  3:2231546/151‑1 (MQ=255)
                                                                                           ccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGGC‑CCGTTTGCCCCGCGCCGGGGGGTGCGCCGCCCG‑CAAAGGCCGATCACCGTGGCATCAGTGGTAAAACTTTTATTTATCCGGCAGTGTTGcgg                                                            >  1:2008573/1‑150 (MQ=255)
                                                                                           ccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGcgt                                                            <  1:1270898/151‑1 (MQ=255)
                                                                                            cgcgATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGcgtc                                                           <  7:1920926/151‑1 (MQ=255)
                                                                                             gcgATGACAAACAGAGTCAGCCCCGGGACCGCAAGTGGGAAGATTCGCCGGGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGGGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGcgtcg                                                          <  6:231365/151‑1 (MQ=255)
                                                                                                aTGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCa                                                       <  3:67749/151‑1 (MQ=255)
                                                                                                   aCAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CCGTTTACCGCCCGCCGGGTGAAGAGACCCCGG‑AAAAAGCCGCAAACCGTGGGATCACTGGGTAAAATTTTAATTATGCCGAAGTGTTTCGGGGTCAGcc                                                    >  6:1017979/1‑150 (MQ=255)
                                                                                                    cAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGt                                                   <  5:2251957/151‑1 (MQ=255)
                                                                                                     aaaCAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGGC‑CGGTTTGCCGCCCGCCGGGTGAGGAGCCCCCGG‑CAAACGCGGCTCACGGTGGGAACAGTGGTTAAAATTTTTTTGATGCGGAAGAGTTTCGTCGTCAGCGTg                                                  >  4:330126/1‑151 (MQ=255)
                                                                                                     aaaCAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTg                                                  <  3:2049599/151‑1 (MQ=255)
                                                                                                         agagTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGGCAGCGTGCGga                                              <  2:2005906/151‑1 (MQ=255)
                                                                                                             tCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGGCC‑CGGTTTGCCCCGCGCCGGGGGGTGAGGCGCCCG‑CAAAGGCCGCTCACGGTGGGCTGAGTGGTTAAAATTTTTTTTATCCGGCAGTGTTTGGTCGTGAGCGTGGGgcaga                                           >  1:968590/1‑150 (MQ=255)
                                                                                                               aGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAAc                                        <  1:4206/151‑1 (MQ=255)
                                                                                                                 ccccGTGACCGCAAGTTGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAAccc                                      <  6:1559354/151‑1 (MQ=255)
                                                                                                                     gTGACCGCAAGTGGGAAGATTCGCCGTGGCGCC‑CGGTTTTCCGCGCGCCGGGTGGTGAGGCGGCGG‑ATAAGGCGGCTGACGGTGGGGTGAGTGGTTAAATTTTTTTTTGTTCGGAAGTGTTGGGTCGTGAGCGTGGGGTAGCAAACcgcgg                                  >  5:217656/1‑150 (MQ=255)
                                                                                                                       gACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTGCCGCGCGGCGGGTGGTGAGACGCCGG‑GAAAGGCCGCTCCCGGGGGCATCAGTGGGTAAAGTTTTTTTGATACGGCAGTGTTGCGTCGGGAGCGTGGGGCAGCAACCCGCGTCt                                >  3:873364/1‑151 (MQ=255)
                                                                                                                          cGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTACCGCGCGCCCGGTGATTAGACGCCGG‑CAAAGGCCGCTGACGGGGGGATGCGTGGTTAAAGTTTTTTTTATGCGGCAGTGTTTCGTCGTGAGCGTGGGGGACCAACCcgcggcgtcc                             >  2:816308/1‑145 (MQ=255)
                                                                                                                            cAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGc                           <  5:1720152/151‑1 (MQ=255)
                                                                                                                            cAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGc                           <  5:519663/151‑1 (MQ=255)
                                                                                                                            cAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGc                           <  7:582629/151‑1 (MQ=255)
                                                                                                                             aaGTGGGAAGATTCGCCGTGGCGCC‑CGGTTTGCCGCGCGCCGGGTGGTGAGCCGCCGG‑CAAAGGCCGGTGACGGTGGGCTGAGTGGGTAAAGTTTTATTGTTGCGGAAGTGTTGGGGGGTGAGCGGGGGGCAGAAACCcgcgggttcgggg                          >  3:1243693/1‑142 (MQ=255)
                                                                                                                               gTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGaa                        <  1:749338/151‑1 (MQ=255)
                                                                                                                                 gggAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGaaaa                      <  7:1268544/151‑1 (MQ=255)
                                                                                                                                 gggAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGaaaa                      <  5:1429524/151‑1 (MQ=255)
                                                                                                                                 gggAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGaaa                       <  8:553924/150‑1 (MQ=255)
                                                                                                                                  ggAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAACGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAAt                     <  7:378550/151‑1 (MQ=255)
                                                                                                                                   gAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATg                    <  5:97342/151‑1 (MQ=255)
                                                                                                                                    aaGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGc                   <  5:448567/151‑1 (MQ=255)
                                                                                                                                    aaGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGc                   <  6:2112626/151‑1 (MQ=255)
                                                                                                                                      gATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCAt                 <  4:1474013/151‑1 (MQ=255)
                                                                                                                                      gATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCAt                 <  2:1696245/151‑1 (MQ=255)
                                                                                                                                           gCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATTAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTTATCCGGAAGTTTTGCGTCGTCAGCGTGGGGAAGAAACCCGCGTCTACGGCGAAAAATCATGta              >  4:1152043/1‑148 (MQ=255)
                                                                                                                                              gTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGcc         >  2:712477/1‑150 (MQ=255)
                                                                                                                                              gTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCa         <  5:1065952/151‑1 (MQ=255)
                                                                                                                                                ggCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCAc        <  4:587490/150‑1 (MQ=255)
                                                                                                                                                ggCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACt       >  2:187283/1‑151 (MQ=255)
                                                                                                                                                  cgcA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACttt     <  8:2019494/151‑1 (MQ=255)
                                                                                                                                                  cgcA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACttt     <  2:1159593/151‑1 (MQ=255)
                                                                                                                                                   gcA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACtttt    <  8:1029002/151‑1 (MQ=255)
                                                                                                                                                   gcA‑CGGGTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACtttt    <  5:67242/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
ATGATTCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCA‑CGGTTTCCCGCGCGCCGGGTGATGAGACGCCGG‑AAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACTTTTCC  >  DH1/1165295‑1165594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑