Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,591,2610CA23.8% 971.8 / 163.7 483intergenic (+27/+233)ECDH1_RS12810/ECDH1_RS12815tRNA‑Tyr/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (166/200);  new base A (61/53);  total (229/254)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 502 total aligned reads displayed.

CACCATCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCAC  >  DH1/2591111‑2591410
                                                                                                                                                      |                                                                                                                                                     
    aTCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTGCCAACg                                                                                                                                                   >  1:1576682/1‑151 (MQ=255)
      ctcAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCAAACGa                                                                                                                                                  >  4:15663/1‑150 (MQ=255)
        cAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATGTGCCGTGATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGaa                                                                                                                                                 <  3:522647/149‑1 (MQ=255)
        cAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTACCAACGAATc                                                                                                                                               >  6:158664/1‑151 (MQ=255)
         aaGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCACCGAATcc                                                                                                                                              >  3:1078890/1‑151 (MQ=255)
           gCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTGCCCAGCGAATCCGc                                                                                                                                            >  4:1802342/1‑151 (MQ=255)
               tACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAAtt                                                                                                                                        >  1:1331640/1‑151 (MQ=255)
               tACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAAtt                                                                                                                                        >  6:396413/1‑151 (MQ=255)
                  atCAATATTGGAATTAGCGCTTGGGGGGTACCCGAGCGGCCATAGGGAGCAGAATGTCAACGTGGCGTCATCCTCTTCGATTGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTaaa                                                                                                                                     <  5:1356459/150‑1 (MQ=16)
                   acAAACTTTTAATTTCCGCTGGTGGGGTTACCGAGAGGACAATGCGAGAAGAATGTAAATCTGCCGTCATCGACTTCGAATGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAat                                                                                                                                    <  4:479197/150‑1 (MQ=21)
                   acAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAATGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAat                                                                                                                                    <  7:1054102/150‑1 (MQ=255)
                    cAATATTTTAATTAGCCCTGGTTGGGTTCCCGAGCGTGCTTAGGGTCCAGACTGTAAATGTCGCGTGATGGACTTAGATGGTTGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAat                                                                                                                                    <  2:2226610/150‑1 (MQ=2)
                    cAAATTTTTAATTACCCCTTGTGGGGATCCCGAGCGTACAAAGGCAGCAGAATGTTAAGCAGCCGTCATCGACTTCGAAGCTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAata                                                                                                                                   <  3:218083/151‑1 (MQ=25)
                      aaaTTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAGCAAGCGAATCCGCAATCAAATAtt                                                                                                                                 >  2:916787/1‑151 (MQ=255)
                       actttGAATTACCCCTGTTGGTGAACCCGAGCCCCCAAAGGGAGCAGACACTAACTCTCCCGTCATCCCCCTCGACCGCTCCAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTc                                                                                                                                <  1:142328/149‑1 (MQ=18)
                       actttGAAGTACCCGTGGTGGTGTACCCGATCTCCCCAAGCGAGAAGACACTACATCAGCCGTCATCTCCGTCGCCGGGTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTc                                                                                                                                <  5:1272500/149‑1 (MQ=16)
                       aaTTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCCACGAATCCGCAATTAAATATTc                                                                                                                                >  2:1657532/1‑151 (MQ=255)
                       aaTTTCAATTACTCCGGGTGGGGTTCCTGATCGGACAAAGGCAGCAGAATGGTACTCTGGGGTGAGCTTCTTCGAATGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTc                                                                                                                                <  4:814386/151‑1 (MQ=16)
                         tttGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATAAAatatacat                                                                                                                              >  6:2022718/1‑147 (MQ=255)
                         tttCAATTACCCCTGGTGGGGGTCCCGAGCGCCCCAAGCGAGCAGAATCTAAATCTGCCGGGATCCTCTTAGATCGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAatat                                                                                                                                  <  5:2023747/147‑1 (MQ=9)
                            caaTTACCCCTGGGGGGGGTCCCGAGCGGCCAAAGGGACCAGACTGTAACTCTCCCGGCATCGACTTCGAACGGTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGccc                                                                                                                           <  1:917440/150‑1 (MQ=18)
                             aaTTAGCGCTGGTGGGGTTCCCGAGAGTCCTTAGGGAGCAGACTGTTAATCTTGGGTGATGTTCTTCGATTGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGccc                                                                                                                           <  4:958620/150‑1 (MQ=18)
                              aTTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCACCGAATCCGCAATGAAATATTCTGCCCAt                                                                                                                         >  1:1216032/1‑151 (MQ=255)
                                tatcgcttctggGGTTCCCGAGCGGCCTAAGGGTCCAGAAAGTACATCTCGCGGCAACGACTAAGATCTTTGGTATCCTTCCCCCACCACCATCACTTTCCAAAGTCCCTGAACTCTCAATCGAATCCGCAATCAAATATTCTGCCCATg                                                                                                                        <  2:2184053/141‑1 (MQ=2)
                                aaCCCCTGGGGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATg                                                                                                                        <  7:1292770/149‑1 (MQ=255)
                                   cgcTGGTGGGGTGAGCGAGAGCCCAAAGGGAGCCGCATGTAAATCTCCCGTGATGTACTTCGAATGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCggg                                                                                                                    <  1:1960699/149‑1 (MQ=18)
                                    ccTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCgggg                                                                                                                   >  6:212666/1‑151 (MQ=255)
                                     cTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTGCCAACCGAATCCGCAATTAAATATTCTGCCCATGCgggg                                                                                                                   >  3:1539633/1‑150 (MQ=255)
                                     cTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCCACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCATCGAATCCGCAATAAAATATTCTGCCCATGCgcgg                                                                                                                   >  1:1659738/1‑150 (MQ=255)
                                      gggtggGGTTCCCGAGCGGCCAACGGCCGCAGACTGTAAATCTGCCGGCACCGACCTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGaa                                                                                                                 <  7:358862/150‑1 (MQ=25)
                                       ggtggGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGaa                                                                                                                 <  1:666021/150‑1 (MQ=255)
                                          ggggTTCCCGAGAGGCCAATGGGAGCAGACTGTAAATCTGCCGTGATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGAt                                                                                                             <  2:1215037/151‑1 (MQ=18)
                                            ggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGCCTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATga                                                                                                           <  7:421684/151‑1 (MQ=255)
                                             gTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAAATACCAAACGAATCGTCAATTAAATAATATTCCAATGCTGGGAAgaaag                                                                                                            >  6:1052276/1‑145 (MQ=18)
                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAAATACCAAACGAATCCTCAATTAAATATTATTCCAATGCGGGGAAGGAtt                                                                                                            >  8:455536/1‑147 (MQ=21)
                                                cgcGAGCGGCCTAAGGGAGCTGAATGTAAATGATCGGTGATGGTCTTAGATGGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAg                                                                                                       <  4:309626/149‑1 (MQ=1)
                                                cccGAGCGCCCAAAGGCACCCGAATGTAAATCTGCCGTCATCGCCTTCGACCCTACGAATCCTTCCCCCACCACCATCACTTTCAAAACTCCCTGAACTTCCCAACGAATGCGCAATTAAATATTCTGCCGATGCGGGGAAGGATGAGaa                                                                                                        <  3:1338364/150‑1 (MQ=18)
                                                  cGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCt                                                                                                     <  5:1573782/151‑1 (MQ=255)
                                                  cGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCAAACGAATACGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCt                                                                                                     >  8:2268501/1‑151 (MQ=255)
                                                  cGAGAGTCCTAAGGGAGCAGACTGTTAATCTCCCGTCATAGACTTCGAATGGTCGAATCCTTCCCCCACCAGCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGTAAGGATGAGAAGCt                                                                                                     <  2:1011465/151‑1 (MQ=18)
                                                   gAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAATACCATGAGCTTCCGAAAGCGTAAGCAATTAAATATAATTCGAATTCGGGGAAGGAGGAGAATCtt                                                                                                    >  2:718888/1‑151 (MQ=14)
                                                    aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATAAATTTCAAAAGTCACTGAACTTCCCAACGAATc                                                                                                                                               >  8:164066/1‑107 (MQ=255)
                                                      cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAAc                                                                                                                                                    <  2:2020223/100‑1 (MQ=255)
                                                        gCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGAcc                                                                                               <  7:2259532/151‑1 (MQ=255)
                                                         ccAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAACCGAAACCGCAAAAAAAAATACTGCACATACAGAGAATGATGAGAAGATTAGaac                                                                                               >  2:1874881/1‑148 (MQ=17)
                                                         ccAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCAACCACCACCATCACTTTCAAAAGTCCCTCAACTTCCCAACGAATCAGCAATTAAATATTCTGACCATGCGGGGAAGGATGAGAACCTTCGAca                                                                                               >  1:2279987/1‑149 (MQ=25)
                                                          cAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTAGagcgg                                                                                             >  1:443400/1‑147 (MQ=255)
                                                          cAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTCCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTaaa                                                                                                                                     <  3:196217/111‑1 (MQ=255)
                                                           gctctcccgagcttctAAAGCTCCCGGCATCGACTGCGCCGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAg                                                                                            <  1:1995396/136‑1 (MQ=11)
                                                            aagccaGCAGACTGGAAATCTCCCGTGATCGTCTGCGAACGTACGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATg                                                                                                                        <  5:1657935/117‑1 (MQ=11)
                                                            aaGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATAAATATCAAAAGACACTGAAATTCAAAACTAATAATCAAATAAATATTATTCAAATGCGAGGAATGATAAGCAGCTACGACCaatg                                                                                           >  4:1347905/1‑149 (MQ=9)
                                                             aGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGt                                                                                          <  7:1912689/151‑1 (MQ=255)
                                                              gggAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGtt                                                                                         >  3:179874/1‑151 (MQ=255)
                                                                gAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCACAACCGAATCCGCAATCAAATATTCt                                                                                                                               >  5:1525598/1‑111 (MQ=18)
                                                                 aGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGATCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGa                                                                                      <  5:2260718/151‑1 (MQ=255)
                                                                   cAGACTGTAAATCTGCCGTCATCGACTTCGGAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGaa                                                                                                        >  8:1564959/1‑131 (MQ=255)
                                                                    aGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATGAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTc                                                                                   >  5:417228/1‑151 (MQ=255)
                                                                     gACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCAGCTCg                                                                                  >  6:1290331/1‑151 (MQ=17)
                                                                      aaagtaactgttccGTGATCGACTTCGATGGGTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAATCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGa                                                                                 <  8:1049753/139‑1 (MQ=11)
                                                                         gTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTGCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAgcg                                                                              >  1:1045906/1‑151 (MQ=255)
                                                                             aggtGCGGTCATGGTCTTAGAATGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAg                                                                          <  4:1105755/148‑1 (MQ=17)
                                                                              tCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCAGTTTCAAAAGGCCGTGCCGTTCGCACCGAATCGGCAATTAAATATTCt                                                                                                                               >  5:172110/1‑97 (MQ=18)
                                                                              tCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCt                                                                                                                               <  6:171882/97‑1 (MQ=255)
                                                                               gtcccGTGATCGACTTCGACGGGTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCg                                                                        <  5:1888010/148‑1 (MQ=11)
                                                                                    gTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCa                                                                                                                                                      <  3:1223018/68‑1 (MQ=255)
                                                                                    gTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGCCTCGAGCGCCAGCgag                                                                      >  3:404564/1‑148 (MQ=255)
                                                                                    gTCATCGACTTCGAAGGTAATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCgagaga                                                                   <  4:628541/151‑1 (MQ=255)
                                                                                     gcagcgcctgcGAAGGGTCGAAGCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCACCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGagagag                                                                  <  1:825425/141‑1 (MQ=17)
                                                                                        tggACTTCGAATGGTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGc                                                                 <  2:304916/147‑1 (MQ=255)
                                                                                          gACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTg                                                             >  7:2018364/1‑151 (MQ=255)
                                                                                               cGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGGGGTTCGGAGCGAATCCTCAGTTAAATAttat                                                                                                                               >  1:1803548/1‑78 (MQ=17)
                                                                                               cGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCa                                                                                                                                           <  2:153463/68‑1 (MQ=255)
                                                                                                gAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAg                                                       <  5:212928/151‑1 (MQ=255)
                                                                                                 aaGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAgg                                                      <  2:1756900/151‑1 (MQ=255)
                                                                                                 aaGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTc                                                                                        >  2:1333522/1‑117 (MQ=11)
                                                                                                  aGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGc                                                     <  1:2052450/151‑1 (MQ=255)
                                                                                                       cGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCAAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCtt                                                                                                    >  5:940835/1‑99 (MQ=255)
                                                                                                             cTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCACCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGaa                                                                                                        >  7:1683514/1‑89 (MQ=255)
                                                                                                              ttCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCt                                                                                                     >  8:1339757/1‑91 (MQ=255)
                                                                                                               tCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAgg                                        >  6:546045/1‑151 (MQ=255)
                                                                                                               acccccACCACCATCACTTTCAAAAGGCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAgg                                        <  3:302741/150‑1 (MQ=255)
                                                                                                                ccaccaccacgaCCACTTACAAAAGACCCTTACGTGCCCCCCGATCCCCCATTTAATTTTGCGCCCCATGCGGGGAAGGTTGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAggg                                       <  1:1854160/148‑1 (MQ=2)
                                                                                                                 ccccACCACCATCACTTTCAAAAGTCCCTCAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGc                                      <  3:938457/151‑1 (MQ=255)
                                                                                                                      ccaccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgc                                 >  7:1492364/1‑151 (MQ=255)
                                                                                                                       caccaTCACTTTCAAAAGTCCCTGAACTTACCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGCCTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGcacatcg                                >  8:1752703/1‑145 (MQ=14)
                                                                                                                        accaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgc                               >  1:2189067/1‑151 (MQ=255)
                                                                                                                        accaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGaaagaacggaagc                               >  8:2156760/1‑140 (MQ=14)
                                                                                                                          caTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgcgc                             >  6:822809/1‑151 (MQ=255)
                                                                                                                            tCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgcgc                             >  7:2134340/1‑149 (MQ=255)
                                                                                                                             cACTTTCAAAAGTCCCTTAACTTCCAAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGTATGAGAAGCTTGGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAg                           <  4:1516864/150‑1 (MQ=255)
                                                                                                                                 ttCAAAAGTCCCTGAACTTGCCACCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCtt                                                                                                    <  7:604543/73‑1 (MQ=255)
                                                                                                                                 ttCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                      >  5:669458/1‑151 (MQ=255)
                                                                                                                                   cAAAAGTCCCTTAACTTCCAAACGAATCCGAAATTAAATATTCTGCCCATGGGGGTATGTATTATAAGCTTCGACCAAGTTTCGACTCGAGCGCCAGCGATAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGa                     <  8:1227419/150‑1 (MQ=14)
                                                                                                                                    aaaaGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGaa                                                                                                                 <  6:1480350/57‑1 (MQ=255)
                                                                                                                                     aaaGTCCCTGTACTTCCCAACTAATCCGAAATTTAATATTCTGCCCATGCGGGGAAGTATGAGAAGCTTGGACAAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTa                  <  4:2132379/151‑1 (MQ=14)
                                                                                                                                          cccTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt             <  3:881546/151‑1 (MQ=255)
                                                                                                                                           ccTTAATTTCCAAACCAATCCTAAATAAAATTTTCTGCCCTTG‑GTGTATGTATTATACGCTTCTACAAAGGTTCTACTCTAGCGCCCGCTATAGAGCGTTGCCGAAGGAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc           <  8:1970779/151‑1 (MQ=2)
                                                                                                                                                  ttCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccac     >  1:310014/1‑151 (MQ=39)
                                                                                                                                                  atCCCAACTAAGCCGAAATAAAATATGCTGCCCATGGGGGCAAGGATTAGAAGCTTCGACCAAGGTCCCACGCGCGCGCCCCCGAGAGCGCGTTGCCCCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccac     <  7:1725501/150‑1 (MQ=11)
                                                                                                                                                    cccAACGAATCCGCAATTAAATATTCTGAGCAAGCGGGGCAGCAAGAGAAGCTTCCAGTAAGGATAGACTCGCGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGCAGCGCGCCGCGCTGCGTCCTCCTTgcccgccgc   >  7:726885/1‑146 (MQ=2)
                                                                                                                                                      |                                                                                                                                                     
CACCATCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCAC  >  DH1/2591111‑2591410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑