Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC23.4% 808.1 / 77.4 403G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (86/222);  new base C (93/1);  total (179/224)
Fisher's exact test for biased strand distribution p-value = 2.12e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 409 total aligned reads displayed.

CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447254‑3447553
                                                                                                                                                     |                                                                                                                                                       
 cACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAcc                                                                                                                                                       <  4:286429/151‑1 (MQ=255)
      tAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGccg                                                                                                                                                  >  4:2092228/1‑150 (MQ=255)
       aGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑t                                                                                                                                                 <  1:1039811/150‑1 (MQ=255)
       aGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑Tc                                                                                                                                                <  7:1433648/151‑1 (MQ=255)
           cATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑Tccccc                                                                                                                                            >  2:138884/1‑151 (MQ=255)
            aTGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCa                                                                                                                                           <  2:859358/151‑1 (MQ=255)
             tGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCGCC‑Gccccccc                                                                                                                                          >  2:58822/1‑149 (MQ=255)
             tGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑Gccccccc                                                                                                                                          >  6:1869674/1‑149 (MQ=255)
               gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCa                                                                                                                                        <  6:331488/151‑1 (MQ=255)
                 ggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCata                                                                                                                                      <  4:674943/151‑1 (MQ=255)
                  gcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCatat                                                                                                                                     <  4:1746364/151‑1 (MQ=255)
                  gcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCatat                                                                                                                                     <  2:1881826/151‑1 (MQ=255)
                   cATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATg                                                                                                                                    <  1:1238964/151‑1 (MQ=255)
                    aTCTTCCAAATCGGCCTGTAACCATTGCCGTGCATCGCAAAAATCTTCGCAGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑GGCCCCacgataagg                                                                                                                                   >  4:428464/1‑143 (MQ=255)
                        tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGcac                                                                                                                                 <  3:546254/149‑1 (MQ=255)
                            caTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCCCCCCATc                                                                                                                           >  7:594211/1‑151 (MQ=255)
                            caTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATc                                                                                                                           <  7:2019616/151‑1 (MQ=255)
                                ggCCTGGAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATccagc                                                                                                                       <  8:1480109/151‑1 (MQ=255)
                                 gCCTGTAACCAGTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATccagcc                                                                                                                      <  3:1761810/151‑1 (MQ=255)
                                  ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATccagccg                                                                                                                     <  2:1685716/151‑1 (MQ=255)
                                      tAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCCGCATATGCCCCACATccagccgcccc                                                                                                                 >  4:177594/1‑149 (MQ=255)
                                        aCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATccagccgccagcc                                                                                                               <  5:2233310/151‑1 (MQ=255)
                                        aCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GACCCCCGCATATGCAACCCATccacccgccagcc                                                                                                               >  6:540497/1‑151 (MQ=255)
                                           aTTGCCGTGCATCGCCAAAATGTTCGCAGACAATATACGCTTCCGGCTGGGGTTCTTTCGCCGCTTCGGTGATCCCTGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTc                                                                                                            <  7:1681026/151‑1 (MQ=255)
                                             tGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCATATGCCCCACAACCACCCGCCAGCCGTcc                                                                                                           >  1:1647177/1‑150 (MQ=255)
                                              gCCGTGCATCGCGAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                         <  2:1556331/151‑1 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                         <  4:1428622/151‑1 (MQ=255)
                                                cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                       <  5:798776/151‑1 (MQ=255)
                                                cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑TCCCCCACCAAATGCACCACATACCGCCGCCAGCCCTCCata                                                                                                        >  4:1995961/1‑150 (MQ=255)
                                                 gTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATAtt                                                                                                      <  8:1029896/151‑1 (MQ=255)
                                                   gCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTcc                                                                                                    <  1:2194081/151‑1 (MQ=255)
                                                    cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCa                                                                                                   <  2:2201811/151‑1 (MQ=255)
                                                     aTCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑ACCCCCCCCAAATGCACCCCAACCACCCGCCCCCCGTCCATATTacac                                                                                                  >  5:1310578/1‑151 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TGCCCCACCATATGGCCCACATAAAGACGCCAGCCGGCCATATTCCAccg                                                                                                >  7:186099/1‑149 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCCAATCCAGCCCCCAGCCGTCCATATTACACgg                                                                                                >  2:1228167/1‑151 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACgg                                                                                                <  5:157669/151‑1 (MQ=255)
                                                        gCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGGGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgc                                                                                               <  7:2161199/151‑1 (MQ=255)
                                                        gCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCCCTTCGCTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCCCAAATTCAACCCCTACCGCCCCCACCCGGGCATAATCAACGgc                                                                                               >  5:2274527/1‑151 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                              <  3:10450/151‑1 (MQ=255)
                                                           aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCt                                                                                            <  8:692159/151‑1 (MQ=255)
                                                             aaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTGCATATTACACGGCGCttt                                                                                          >  3:1180043/1‑151 (MQ=255)
                                                              aTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCAGCATATGCCACCCAGCCAGCCGCACGCGGTCCAAAttaaccgcccgttt                                                                                          >  6:906601/1‑138 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGACCCCCCCCAATTGC‑CCCCCTCCCCCCCCCCGCCGGCCAAATTTCACGGCGCgttgac                                                                                       >  5:514450/1‑148 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCa                                                                                        <  3:1390669/150‑1 (MQ=255)
                                                                  tCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCCTATGCCCCACATACAGCCGCCACCCGTCCCTATTACACGGCGCGTTCAGcc                                                                                     >  7:320629/1‑151 (MQ=255)
                                                                      cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCAGATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCAGATGCACCACAACCAGCCGCCCCCCGTCCCGATTCCACGGCCCGTTCACCCAGTg                                                                                 >  6:210080/1‑151 (MQ=255)
                                                                           atatACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGa                                                                            <  6:688657/151‑1 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCACCCCATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTGTCAGCCAGTGGCGGAc                                                                           >  4:1684179/1‑151 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATATGCCCCACCTCCCGCCGCCCGGCGGGCCTATTCCCCAGCGCTTTCCGGCAGTGGCGGAc                                                                           >  2:1995636/1‑151 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCATATGGACCCCAAACAGCCCCCACCCGGCCCTTTTACACGGCGCGTTGAGCCAGTGGCgggc                                                                           >  8:407061/1‑149 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑ACCCCCCCCATATTCACCACAGCCACCCCCCCGCCCGCCATATACCCCCGCGCGTTCAGCCCGGGGCggcc                                                                           >  5:456826/1‑149 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑ACCCCCACCATATTCCCCCCAAACAGCCGCCCCCCCTCCAAATTACACCGCGCGTTAAGCCACTGGCggcc                                                                           >  2:1100100/1‑149 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑TCCCCCCCCATATGCACCACAAACACCCGCCCCCCCTCCCTATTACACCGCCCGTTCACCCACTGGCgggc                                                                           >  7:406885/1‑149 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCCCCACATCCCGCCCCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCggcc                                                                           >  6:1582347/1‑148 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGAc                                                                           <  8:1281326/150‑1 (MQ=255)
                                                                                cGCTTCCGGCTGGGTTGCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATa                                                                       <  1:23306/151‑1 (MQ=255)
                                                                                  cTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCACCATATGCACCACATCCACCCGCCAGCCCTCCCAATTACACCCCGCTTTCAGCCACTGGCGGACAATACt                                                                     >  3:1604626/1‑151 (MQ=255)
                                                                                    tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCCTCCATATTCCACCGCGCGTTCAGCCATTGGCGGACAATACGGt                                                                   >  8:1868418/1‑151 (MQ=255)
                                                                                       ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCAGCATATGCCCCACATACAGCCGCCACCCGGCCATATTACACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCtt                                                                >  3:27257/1‑151 (MQ=255)
                                                                                         cTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑AGCCCCCGCGAATGCACCACCTCCACCCCCCCACCGAACATATTACCCCGCGCGTTTACCCAGAGGCCGGCAATaaagggatac                                                              >  2:732153/1‑142 (MQ=255)
                                                                                         cTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACCGCGCTTTCAGCCAGTGGCCGGCAATACTGTCTTcc                                                              >  8:1738473/1‑151 (MQ=255)
                                                                                              tttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCCCCCTATTCACCACAAACCCCCGCCCGCCCGGCAAATTACAACGCGCGTTGAGCCCCTGGCGGGCAAAACTGTCTTGCCcccg                                                         >  7:852247/1‑151 (MQ=255)
                                                                                              tttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCCCATCCAGCCGCCCGCCGTCCATATTCCCCGGCGCTTTCAGCCAGTGGCGGCCAATACTGTGTTCCCcccg                                                         >  5:975859/1‑151 (MQ=255)
                                                                                               ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                         <  2:957037/150‑1 (MQ=255)
                                                                                               ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                         <  1:589774/150‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                       <  6:1237517/148‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                       <  8:307536/148‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                     <  5:2024917/150‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATa                                                      <  3:2105947/149‑1 (MQ=255)
                                                                                                    tcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑TCCCCCAGCATATTCACCACCTCCCGCCGCCAGCCGTGCATATTCCACGGCGCGTTCAGCCAGTGGCGGCCCATACTGTCTTTCCCGCGCTAAAt                                                   >  5:1145912/1‑151 (MQ=255)
                                                                                                     cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAt                                                   <  5:1435225/150‑1 (MQ=255)
                                                                                                      gccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAttt                                                 >  1:2116850/1‑151 (MQ=255)
                                                                                                         gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCATATGCACCCCATCCCGCCCCCCGCCGGCCATATTACACGGCGCGTTCAGCCACTGGCGGCCCATACAGTGTTACCCCCGATAAATttcat                                              >  1:416455/1‑151 (MQ=255)
                                                                                                           ttCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCCTATGGCCCCCCACCCGCCCCCCCCCGGCCCAATTACACCGCGCGTTCACCCAGGGGCGGCCCATAAGGTGTTCCCCGCGCTAAATttcattc                                            >  1:743397/1‑151 (MQ=255)
                                                                                                           ttCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCCGCATATGCACCACATCCAGCCGCCACCCCGCCAAATTACACGGCGCGTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCCCGATAAATttgattt                                            >  8:807489/1‑150 (MQ=255)
                                                                                                             cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                           <  7:905185/150‑1 (MQ=255)
                                                                                                             cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                           <  5:1012431/150‑1 (MQ=255)
                                                                                                              ggTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCAcc                                         <  5:1870485/151‑1 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGa                                      <  7:130202/151‑1 (MQ=255)
                                                                                                                    cccGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GGCCCCAGCAAATGCCCCCCAAACCCCCGCCCCCCGGGCATATTCCCCCGCCCTTTCAGCCACTGGCGGCCAAAACGGGGGTCCCCGCCATAAAATTCATTCACCacacgt                                   >  2:265001/1‑149 (MQ=255)
                                                                                                                    cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATT‑CACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                  <  8:2105581/151‑1 (MQ=255)
                                                                                                                    cccGGCAACCTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACt                                    <  6:2265828/150‑1 (MQ=255)
                                                                                                                     ccGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCCCCATATTCCCCACCACCCCCCCCCCGCCCGCCCTATTACCCCGCCCGTTAAGCCACTGGCGGGCAATACGGTCTTACCCCCGCAAAATTTGATTGACCCGACctt                                  >  4:1116774/1‑151 (MQ=255)
                                                                                                                     ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCAGATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                  <  6:993544/151‑1 (MQ=255)
                                                                                                                            cGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCAGCATATTCCCCCCATACAGCCCCCAGCCGTGCCTATTGCACGGCGCTTTGAGCCAGTGGCGGGCAATAAGGTCGTGCCCGCGCTAAATTTGATTCACCAGCCTTTCCGACTg                           >  1:1526511/1‑151 (MQ=255)
                                                                                                                            cGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTg                           <  5:1819008/151‑1 (MQ=255)
                                                                                                                             gTGCTGCATATTATTGCGCGCCCCACCCCCC‑GCCCCCACCATATTCCCCACATCCACCCGCCAGCCCGCCATATTCCACCGCGCGTTCAGCCAGTGGCGGCCAAAACTGTGGTGCCCGCCATAAATTTAATTAACCACACGTTCCGAcgg                           >  5:362710/1‑148 (MQ=255)
                                                                                                                                  gcATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAAt                      <  3:2039366/150‑1 (MQ=255)
                                                                                                                                   cATATTATTGCTCGACCCACCCGCC‑TCCCCCACCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATcc                    <  3:428512/151‑1 (MQ=255)
                                                                                                                                    atatTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCa                   <  3:344105/151‑1 (MQ=255)
                                                                                                                                      attattGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGc                 <  3:2246249/151‑1 (MQ=255)
                                                                                                                                        tattGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCt                <  1:1940456/150‑1 (MQ=255)
                                                                                                                                        aattGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCtt               <  8:487906/150‑1 (MQ=255)
                                                                                                                                         attGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTc              <  6:1676040/151‑1 (MQ=255)
                                                                                                                                            gcgcgcCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCgg            <  3:130069/150‑1 (MQ=255)
                                                                                                                                            gcgcgcCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTGTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCgg            <  6:1418875/150‑1 (MQ=255)
                                                                                                                                              gcgcCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCaa         <  1:1406327/151‑1 (MQ=255)
                                                                                                                                                gcCCCACCCGCC‑TCCCCCAGCATATGGCCCACAACCAGCCCCCCGCCGTCCATATTACACGGCGCTTTCAGCCCGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTGATTCACCAGACTTTGCGACTGATAATACAGCTTCGGCAAGc       >  4:2206735/1‑151 (MQ=255)
                                                                                                                                                 ccccACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCt      <  3:657655/151‑1 (MQ=255)
                                                                                                                                                     aCCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   >  3:1816090/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447254‑3447553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑