Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,448,5260AC29.8% 907.2 / 150.0 524intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand):  ref base A (298/67);  new base C (1/154);  total (300/224)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 544 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676
                                                                                                                                                      |                                                                                                                                                      
 gCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc                                                                                                                                                       >  7:1876005/1‑151 (MQ=255)
 gCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc                                                                                                                                                       >  5:1243469/1‑151 (MQ=255)
  cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                      <  6:1567577/151‑1 (MQ=255)
   aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACTTCATATTTCCGCTGTGATGAATATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGATGAGGACgg                                                                                                                                                     <  2:1088714/151‑1 (MQ=255)
   aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgg                                                                                                                                                     <  7:343177/151‑1 (MQ=255)
       gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                                  <  8:1245215/150‑1 (MQ=255)
       gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                                 >  8:2103322/1‑151 (MQ=255)
        aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCg                                                                                                                                                >  5:2259743/1‑151 (MQ=255)
            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                                 <  2:568659/146‑1 (MQ=255)
             tgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATa                                                                                                                                            >  7:2269201/1‑150 (MQ=255)
               ctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGCAGAACt                                                                                                                                         >  5:911995/1‑151 (MQ=255)
                     ttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                    >  7:496936/1‑150 (MQ=255)
                     ttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                    >  6:105501/1‑150 (MQ=255)
                     ttAAAATATGGGGGCACCGGCAGGTGCCATGAATTTAACATCATAATTCCGCTGTGATCAAAATCTGATCACCTTGCCATAGCATATTGCAGGATAACTCATCTTGTTAAGGATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                    <  1:701397/150‑1 (MQ=255)
                       aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  2:1512833/1‑151 (MQ=255)
                       aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  2:235461/1‑151 (MQ=255)
                        aCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  5:1623683/1‑150 (MQ=255)
                         cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  3:351634/1‑149 (MQ=255)
                         cAAATGGGGGAACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGTATCACTCATCATCTGAAGGATATTTGGGGTAGGAGGACGGGGGCGGCTAACTTTAGGAAt                                                                                                                                 >  3:1609846/1‑149 (MQ=255)
                          ataTGGGGGCACCGGCATGTTCAATTAATTTAAAATCATAATTCCCCTTTTATTAAAATCTGATCACGTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGc                                                                                                                              <  7:636373/149‑1 (MQ=255)
                          aaaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGc                                                                                                                              >  1:2066907/1‑151 (MQ=255)
                           aaTGGGGGCACCGGCAGGTGCAATGCATTTAACATAATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGACGTATATTTGGGGGAGGCGGCGGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                             <  1:1321158/151‑1 (MQ=255)
                            aTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                            >  6:1629600/1‑151 (MQ=255)
                            aTGGGGCCCCCGGCCGGGGCCATGCATTTAACATCATAATTCCCCTGTGATCAAAATCTGATCACCTTGCCATAGCCTAATCCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                            <  2:648434/151‑1 (MQ=255)
                              gggggCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCgg                                                                                                                           >  4:280426/1‑150 (MQ=255)
                                gggCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATc                                                                                                                        >  5:2123417/1‑151 (MQ=255)
                                   cACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTCATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGACGAGGACGCGGGCGCATAACTTTCGGAATAGCCGTATAAgc                                                                                                                     >  6:1828930/1‑150 (MQ=255)
                                     ccGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                                   >  8:1666056/1‑151 (MQ=255)
                                      cggctggtgcctacaaTTTAACATCATAATTCCCCTGTTATGAAAATCTGATCACGTTGCCATAGCCTACTGCAGGATCACTCATCCTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                                  <  2:1350862/136‑1 (MQ=255)
                                      cGGTAGGTGGAATGAATTTAACATCATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATCGCCTCATGCAGGATCACTCATCATGTGAAGTATATGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                                  <  3:202197/151‑1 (MQ=255)
                                        gCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtc                                                                                                                >  8:1863814/1‑151 (MQ=255)
                                         cAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                               >  3:1949010/1‑151 (MQ=255)
                                          agctGCCATCCATTAAACATCATAATTCCCCTTTGATCAAAATCTCATCACCTTGCCATACCCTAATGCAGCATCACTCATCATCTTAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcct                                                                                                              <  4:987448/148‑1 (MQ=255)
                                           ggtgggagggaTTTAACATAATAATTCCCCTGTGATCAAAATCTTATCCGCTTCCCATAGCCTAATGCAGGATCACTCTTCTTCTGCTTTTTTTTGGGGGGAGGTCGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctc                                                                                                             <  2:711685/141‑1 (MQ=255)
                                            gggCCATTAATTAAAAATCATAATTCCCCTGTTATTAAAATCTGATCACCTTGCCATCGCCTAATGCAGGATCACTCATCATCTGAATGATATTTGGGGGCGGAGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctcc                                                                                                            <  8:2251566/149‑1 (MQ=255)
                                               cgattcATTTAAATTCATCATTCCCCTGTACTCAAAAGCTCACCACCTTTCCATACCCTATTGCAGCTACACTCCTCATCTGAAGTATATTTGGGGGCGGCGCCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGa                                                                                                         <  6:2006716/146‑1 (MQ=255)
                                               ccATTCATATAACAACATAATTCCCCTCAGATCAACCTCCGCTCCCCTCCCCATCCCCTAATCCAGCATCACTCATCATCTGCCGGATCTGCGCGCGCGCCGCCCGGCCCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGa                                                                                                         <  2:1200535/151‑1 (MQ=255)
                                               ccATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                          >  1:907721/1‑150 (MQ=255)
                                                cATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGa                                                                                                         >  3:1226378/1‑150 (MQ=255)
                                                   gCATTAAACATCATAATCCCCCTCTCATCAAAATCTCACCACCTTGCCATAGCCTAATGCCCCCTCACTAATCCACTCAAGGATATTTGGGGGCGCCGGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                      <  6:766656/150‑1 (MQ=255)
                                                    cATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCgg                                                                                                    >  7:1715736/1‑151 (MQ=255)
                                                    cATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCgg                                                                                                    >  5:39220/1‑151 (MQ=255)
                                                     atatAACATCATAATTCCCCTTTTATAAAAATCTGATCTCCTTGCCATACCCTAATGCATGATCACTCATCATCTGAATTATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   <  4:1372660/148‑1 (MQ=255)
                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCCGc                                                                                                   >  7:148903/1‑151 (MQ=255)
                                                      tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGACCCGGCt                                                                                                  >  3:1465924/1‑151 (MQ=255)
                                                       ttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCtt                                                                                                 >  7:1188553/1‑151 (MQ=255)
                                                       ttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCtt                                                                                                 >  2:2014644/1‑151 (MQ=255)
                                                        aaaCATCATAATTCCCCTGTGAACAAAAGCTCACCACCTTCCCATACCCTAAGGCAGCACCACTCAACATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTa                                                                                                <  1:329296/150‑1 (MQ=255)
                                                         aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTGTCGGAATAGCCGGATCAGGATTTCCTCCTGCACCGGCTTAc                                                                                               >  3:2132464/1‑151 (MQ=255)
                                                           catcatAATTCCCCTTTTATTAAAATCTGATCACATTTCCTTAGCCTAATTCAGGAACACTCATTATCTCATGGATATTTGGGGGAGGCGGTGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAg                                                                                             <  8:1360723/151‑1 (MQ=255)
                                                           caacatAATGCCCCTGTCATCAAACTCTCATCCCCTCGCCATCCCCTAAGCCACGACCACTCATCATCTGAAGGAGATGTGGGGGCCGCGGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAg                                                                                             <  1:10121/148‑1 (MQ=255)
                                                              catAATGCCCCTGTGAACAAACGCTGACCCCCTTGCCACCCCCTAACGCAGCATCACCCATCATCTGAAGGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  1:1134015/151‑1 (MQ=255)
                                                              aatAATTCCCCTGTCATGAAAATCTCATCACCTTGCCATACCCTAATGCAGCATCACTCATCATCTGAATGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  8:680979/150‑1 (MQ=255)
                                                                 aaTTCCCCTGTGATCAAAATCTCATCACCTTCCCATCGCATAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCa                                                                                       <  4:1623658/151‑1 (MQ=255)
                                                                  aTTCCCCTGTGATCAAAATCTGATACCCTAGCCATCGCCTAATGCACGATCACTCATCATCTGAAGGATTTTTGGGGGAGCCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                      <  4:798478/151‑1 (MQ=255)
                                                                       ccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAtt                                                                                 >  2:910954/1‑151 (MQ=255)
                                                                             atcaaaaTCTGATCACCTTGCCATAGCCTAATCCACGATCACTCATCATCTGAAGGATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGt                                                                           <  5:1661946/148‑1 (MQ=255)
                                                                               gaAAATCTGATCACCTTGCCATAGCCTAATGCCGGACCACTCATCATCTGAAGGATATTGGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtt                                                                          <  7:2188830/150‑1 (MQ=255)
                                                                                aaaaTCTGATCCCCTTCTCATACCCTATGGCATCATCACTGATGATCTCTTGCATCCTTGGGGGATGAGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                        <  6:1378482/151‑1 (MQ=255)
                                                                                  aaTCTGAGCACCTTGCCATACCCTAATGCAGGATCACTCATCCTCTGAAGGATATTGGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTca                                                                      <  7:600333/151‑1 (MQ=255)
                                                                                       gagccccTTGCCATACCCTAAGGCCGGAGCCCTCATCATCTGAAGTATTTTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcca                                                                  <  8:292341/145‑1 (MQ=255)
                                                                                       gATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                     <  8:568254/147‑1 (MQ=255)
                                                                                         gcaccggcccAAAGCCAAATGCAGGATCACTCATCATCTGAAGCATATTTGGCGGCGGCGGCCGCGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                               <  1:1639777/143‑1 (MQ=255)
                                                                                          cACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                              >  7:1366401/1‑151 (MQ=255)
                                                                                          cACCTTCCGAAAGCCTACTGCATGATCACTCAACCTCTGAAGGATCTTTGGGGGCGGAGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                              <  3:777012/151‑1 (MQ=255)
                                                                                          cACCTACCCATCGCCTAATGCAGGAGCACACACCATCTGACGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccagc                                                                <  7:2067262/149‑1 (MQ=255)
                                                                                             cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGCATTTCCTCCTGAACCGGCTTACAGAACGCAAGTATTAATGGTTTTcagacagcata                                                             >  6:1246685/1‑146 (MQ=255)
                                                                                              ttGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                           >  3:1395667/1‑150 (MQ=255)
                                                                                               tGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGAATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGAt                                                         >  5:828194/1‑151 (MQ=255)
                                                                                                gCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                        >  4:1482081/1‑151 (MQ=255)
                                                                                                  cATAGCCTCACGCACCACCACTCATCATCTGAAGGATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTATTGGTTTTCAGCCAGCTGACGATCGt                                                      <  4:565312/151‑1 (MQ=255)
                                                                                                   atccccaaaTGCAGGAACACTCATCATCTGAAGGATATTGGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTATAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTg                                                     <  3:930913/144‑1 (MQ=255)
                                                                                                   aTAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTCACGATCGTg                                                     >  2:2121919/1‑151 (MQ=255)
                                                                                                    tAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGCACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                    >  6:243804/1‑151 (MQ=255)
                                                                                                     ccccTAATCCTGGCTCCCTCATCATCTGAAGGATAGTTGGGGGCCGCGCCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTg                                                     <  6:711483/147‑1 (MQ=255)
                                                                                                           aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                             >  4:1754000/1‑151 (MQ=255)
                                                                                                             gCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtgt                                            >  2:1789030/1‑148 (MQ=255)
                                                                                                              cAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTa                                           >  8:846750/1‑150 (MQ=255)
                                                                                                               aGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                          >  1:2241085/1‑150 (MQ=255)
                                                                                                                   tCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTAGGAATAGCCGGATCAGGATTTACTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaaaaac                                     >  4:208304/1‑147 (MQ=255)
                                                                                                                     aCTCATCATCTGAAGGATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCa                                   <  7:1153656/151‑1 (MQ=255)
                                                                                                                        catcatCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                                >  8:1557352/1‑151 (MQ=255)
                                                                                                                           catcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                             >  3:1090703/1‑151 (MQ=255)
                                                                                                                             tcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                            >  8:361521/1‑150 (MQ=255)
                                                                                                                              cTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCa                           >  8:1082876/1‑150 (MQ=255)
                                                                                                                               ggcaggagagtggggggCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         <  5:622695/139‑1 (MQ=255)
                                                                                                                                gAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         >  5:1809047/1‑150 (MQ=255)
                                                                                                                                 aaGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         >  1:1927349/1‑149 (MQ=255)
                                                                                                                                  aGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                      >  6:494420/1‑151 (MQ=255)
                                                                                                                                     atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    >  1:1827904/1‑150 (MQ=255)
                                                                                                                                     atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGACAATCAACAGcac                    >  1:5388/1‑150 (MQ=255)
                                                                                                                                      tttttGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcaca                   <  4:1242193/148‑1 (MQ=255)
                                                                                                                                       atTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGACCGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaac                 >  6:41553/1‑151 (MQ=255)
                                                                                                                                        ggcgggggAGGAGGACGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca                <  3:514088/148‑1 (MQ=255)
                                                                                                                                              ggaggcggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc          <  1:1475306/151‑1 (MQ=255)
                                                                                                                                              ggaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc          >  8:1904514/1‑151 (MQ=255)
                                                                                                                                                   aggaCGGGGGCGGATAACTTTCGGATTAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACAGCCAGCCAATCAACAGCACAACAATCCCCGCGa      >  4:1105183/1‑150 (MQ=255)
                                                                                                                                                    gcaCGGCGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGaaa    <  7:842026/149‑1 (MQ=255)
                                                                                                                                                     gaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAc   >  5:1512650/1‑151 (MQ=255)
                                                                                                                                                     gaCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAc   <  7:1590421/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑