Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8320CA29.1% 190.3 / 14.1 112intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (51/22);  new base A (29/1);  total (88/24)
Fisher's exact test for biased strand distribution p-value = 3.24e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCT  >  DH1/3906684‑3906981
                                                                                                                                                    |                                                                                                                                                     
cgggggggCTCAACTCCCCCCCCTCGCACCAAATTTTTTTTTTATATTGGGCGCACTGGGAGATGTTGTCGGTATTTGTATATGTTTTAGTTTAAGTTGGTATTGCTATTTCGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGa                                                                                                                                                     <  2:2279639/148‑1 (MQ=9)
       ggtCAAGTCCCCCCCCTCGCAGCAAAAACCACGTTGATATTGCTCGCCCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAAACGAGGCGata                                                                                                                                              <  7:114504/149‑1 (MQ=255)
        ccccacgccccccccTCCCACCAACCACCACGTTGAAAGACCTCCCCCTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                             <  8:1892881/144‑1 (MQ=17)
          catgTCCGCCCCCTCGGACCAATAACGTCGTTGATTTTGTTGTGTGTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCGCCCCTCGCACCAAAGGAGGCGATATc                                                                                                                                            <  8:1666132/147‑1 (MQ=14)
                 cccgccTCGCACCAAAAACCTCGTTGTTATAGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAg                                                                                                                                    <  7:502003/151‑1 (MQ=255)
                  cccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCATCAGGTGATAGTGTCCTTAAGGACGTGGGGGTGGCAATCCCCCCCCACGCCACAAAACAGAGGAGATaaaaaaaac                                                                                                                                    >  1:762156/1‑149 (MQ=18)
                   cgccTCGCTCCAACCAACTCGTTTAAATTGCTGCCTCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTa                                                                                                                                  <  3:298011/149‑1 (MQ=17)
                   ccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTACAGTCCCCCCACACGCAAAAAAAGACGTGAAATCAAAAAAAGtt                                                                                                                                  >  4:2229603/1‑150 (MQ=21)
                    cccTCCCACCAACAAACTCGTTTAAATACCTCCCCGTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTaa                                                                                                                                 <  5:612783/151‑1 (MQ=17)
                     ccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTGAAGTCCCCCACCGCGCCCCAAAAAACGCGAGATCaaaaaaattta                                                                                                                                 >  7:60976/1‑146 (MQ=21)
                       tCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTAAAATCCCCCCCCACCCCCCAAAAAACGCAATATaaaaaaaaattaaa                                                                                                                               >  8:2052724/1‑144 (MQ=18)
                        cGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTGAACTCCCCCCCCCCCCCCCACAACCCGCGGGATCAAAAAAAGtttctat                                                                                                                             >  7:1242516/1‑145 (MQ=18)
                          cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGTATAAATCATAAAAAGTAAGAtca                                                                                                                            >  4:2110510/1‑148 (MQ=18)
                           aCCAAAAACCTCGTTGATATTGCTCGCACTGGGTGAAGGTTGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACt                                                                                                                          <  4:763241/151‑1 (MQ=255)
                           aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAAGAACGGAAGAGCAAACAAAAAAAGTacactcacg                                                                                                                          >  3:1967964/1‑143 (MQ=17)
                            ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTCTAGTCCCCCCCCACGCCCCAAACCAGGGGAAATCaaaaaaatttatttggctt                                                                                                                         >  7:2017071/1‑139 (MQ=18)
                            cataaaaCCACGTTGATATTGCTCGCTCTGGGTTAGTGTGGGGTATTTCGACGTCTCGGCTGCGGCTGTTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtg                                                                                                                         <  7:1221168/148‑1 (MQ=16)
                             cAAAAACCACGTTTATATTGCTCGCAGTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGGGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgt                                                                                                                        <  1:2120223/151‑1 (MQ=255)
                             cAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAACGAGAAATCAAAAAAAGTTAGATgcatg                                                                                                                         >  8:1963371/1‑146 (MQ=18)
                              aaaaaCCTCTTTGATATTGCTGTCAGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                       <  8:1678408/151‑1 (MQ=21)
                               agaactcgtttGATATTGCTCGAAGTGTGCGATTGGGTTGTAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGc                                                                                                                      <  1:1994458/142‑1 (MQ=18)
                               aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCCCACCAAATAAGGCTATATCAAAAAAAGttaatttcatgtct                                                                                                                      >  7:929867/1‑138 (MQ=18)
                                 aaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAAAGGGTAAATAAATAAATGTAAGAttaatgatat                                                                                                                     >  6:1570322/1‑141 (MQ=17)
                                 aaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACTATAAATCAAAAAAAGttacatcaatgatttt                                                                                                                    >  2:162568/1‑136 (MQ=17)
                                    cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGAGTTCAAAAAATGTTTGGTGACtgtggtttgt                                                                                                                 >  5:54277/1‑145 (MQ=18)
                                    cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAATTAGTCGGTATCACACAACTGAAGAAGACTGTGcccact                                                                                                                 >  5:2038579/1‑146 (MQ=17)
                                    atcGTTTATATTACTGGCTGTTGGGGCAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAgg                                                                                                                 <  5:507275/149‑1 (MQ=18)
                                    aaCGTTGATATTGCTCGCACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAgg                                                                                                                 <  3:1910720/150‑1 (MQ=255)
                                     aagTTGATATTTCTCTCTTTCGGTTAAGGAGGCTGTTTTGTTATACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGt                                                                                                                <  6:884007/149‑1 (MQ=18)
                                     aCGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAACTCCCCCCCCTCCCACCAAAAAACGTTAAATCCAAAAAAATTAGATGaaagagaaatact                                                                                                                >  4:1060059/1‑139 (MQ=14)
                                            tatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGAGAAATCAAAAATAGTTAGATGCGTGGGGTTAGGTGGCGGa                                                                                                          >  8:1309611/1‑150 (MQ=17)
                                            aatacctcccgctctgCGAAGGTGGCGGAATTTTTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTTGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAATATTACTGTGCGAAGGTGGCGGaa                                                                                                         <  2:2295266/136‑1 (MQ=18)
                                                cctgcacgtgtgCGAAGGTGGCGTAATTTGTAGACGCGCTAGCTTCAGGTCTTAGTGTTCTTACGGACGTGGCGGTGCAAGGCCCCACCCTCGCACCAACCGATCCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTgg                                                                                                     <  3:88802/140‑1 (MQ=255)
                                                  tCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCTAGTTCAAATAAAGTTAGATGACTGTGGGTTGGTGGCGGATTTGGtt                                                                                                   >  7:1251225/1‑150 (MQ=18)
                                                  tCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCCGCCCCAAAAGCGGGGATAGCAAAAAAATTTAGTTGAGTGTTGGTAGGTCGCGGGAATGGtt                                                                                                   >  7:1612310/1‑150 (MQ=14)
                                                     ccctcgtccgatctGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTATGGTTTTTTTGTTTCTAGTCCCCCCCCTCGCACCAAACGAGTAGATATCAAAAAAATTAAGATGACTGTTCGAAGGTGGCGGAATTGGTAGAc                                                                                                <  4:1381574/138‑1 (MQ=21)
                                                      tttcagCGAAGGGGGCGGAATTGTTATACGATGTAGGTTCAGGTGTTCCTCCGCTTCCTCTCGCGCGCTTGAAACTCCCCCCACTCGGCCCAACCGCTCCGATATCAATAAAAGTAATCTGTGTGTGCGAAGGTGGCGGAATTGGTAGAc                                                                                                <  1:1831408/145‑1 (MQ=255)
                                                       cTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGTTAGATAAATAAAAGTTTGATGACTGTTGTTTGTTGGCGGAATTggattaatt                                                                                              >  7:171229/1‑144 (MQ=14)
                                                                      ggAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTAATTCAAATAATGTTTGTTGCATGTGTTTAGGTGGCGGATTTTGTTTAATTGTTAGCTTCAGgtgt                                                                               >  5:28750/1‑151 (MQ=2)
                                                                         aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAAAGTATGATGCCTGTGCTAAGGTGGCGGAATTGGTAGACGGGGTAGCTGCAGGTGtt                                                                              >  5:1425655/1‑149 (MQ=21)
                                                                          ttGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTTGATGCCTGTTGGAAGGTGCCGGAATTGATATACTCCAAATCATCAGGTTTTAgt                                                                           >  5:1811925/1‑151 (MQ=17)
                                                                              tAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGTAATTAAATAAATGTTAGATGAGTGTTTGTAGGTGGCGGATTTGCTATACTAGATAGCATCATGGGTTacgcta                                                                        >  4:2232750/1‑145 (MQ=2)
                                                                                   ccgcTACCTGCAGGCGTGAGTGGTCTTACGGCCGTGGGGCGTCATGTTGCATTTCTCGCCCTAATCGATCCGATTTCAAATAAAGTTTGATGACTGTGCGAAGGTGGCTGAATTGGTTTACGCGCTAGCTTCAGGTGTTAGTGTCCTTACg                                                                  <  8:1164076/150‑1 (MQ=9)
                                                                                     gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATAGGGGATATACAAAAATCTAAGGTACATGTTCGTAGGTGGCGGGATTGGTAGAATAGCGAGCTTCAGGTTGTAGTGTACTTTCggc                                                                >  5:466826/1‑150 (MQ=9)
                                                                                     gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAACAAACGACGCGAAATCCAAAAAACTAACGTGACGGTTGGCAGGTGGCGGAAGTGGGTGAGGCGGTATAATAAGGTGTTAgtgtggggggggg                                                                >  5:1013092/1‑142 (MQ=12)
                                                                                     gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAAAAGAGTGGATATCAAAAAAAGTTTGTTGACTGTTGTTTGGTGGCGGAATTGGTTTACGCTGGAGCATCAGGTTTTActgaaaatagggc                                                                >  7:394471/1‑141 (MQ=9)
                                                                                      cTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCACGCCCCAAAACAGGGGATTTCAAAAAAAGTTTTATGCCTGTTGTTTGGTGGCGGAATTGGGAGACGCGCAAGGGTGACGTGGTATTGTccattaggac                                                               >  7:2000101/1‑143 (MQ=9)
                                                                                       tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCCCACCAAAAGAGGCGATATAAAAAAAATTATTATGACTGTTGGAATGTTGCGGAATTTTTATAACCGGGATCGTCAGGTGTTAGGGTACTTACggccc                                                              >  7:831349/1‑148 (MQ=12)
                                                                                         gCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGTGGGGAGTTCAAAAAAAGTTTGATGAGTGTGGTTAGGTGGCGGATTGAGAATAAGTGATATCATCAGGTGTTAgtctaaaaacggcagtg                                                            >  1:167228/1‑136 (MQ=9)
                                                                                          cTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTTGTTGAATGTTTTTAGGTGGCGGAATTGTTATAAGTGATATCATCATGTGTTActgtaaattaggccgtgg                                                           >  7:1227668/1‑137 (MQ=12)
                                                                                              aGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCACCAAAGGAGGGGATTTCAAAAAAAGTTAGTTGAATGTTGTTTGGTGGCGGAATTTGTATACGAGTTAGCATCAGGTTTTAGTGTAATTAAGGACGTGGGGGt                                                       >  7:1160540/1‑151 (MQ=16)
                                                                                                 tgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAAATCATAAAATGTTAGTTGAATGTTCTTAGGTGGCGGAATATGTATACTGGGGATCTTCAGGTGGTAGTGTCAATACGGCCGGGGGGGTTc                                                     >  5:1093937/1‑150 (MQ=12)
                                                                                                  gtTAGTGTTCTTACGGACGTGGGGGTTCAACTACCCCCCCACCCCCCAAAAGAGGCGATATCAAAAAAAGTAAAATGAATGTTCGTAGGTGGCGGAATTTGAATACGAGCGATCATCAAGTGTTATTGTACTTAAGGACGAGGGGGtgga                                                    >  8:116431/1‑147 (MQ=255)
                                                                                                    tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGAAGGGGATATCAAAAAAAGTTAGATGAATGTTGTAAGGTGCCGGATTTGTTATACGCGATAGAATCAGGTTTTAGTGTAAAATAGGACGTGGGGGttgtagg                                                 >  2:1069189/1‑146 (MQ=16)
                                                                                                    tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTTAGATGAGTGTTGTATGGTGGCGGAATTGGTAGAAGAGATAGCAGCAGGATTAAGTGTAAATAAGGCCGTGGGGGtggaagg                                                 >  2:1058495/1‑145 (MQ=16)
                                                                                                    tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGACGGTATATCAAAAAATGTAAGATCACTGTGAGATCGTGGCGGAATTGATAGAAGGAATAGCTTCAGGATGAAATGTCATTAAGGACGTGGGGCTTCa                                                    >  2:782379/1‑148 (MQ=16)
                                                                                                       tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCCAAAAATTTTAGTTGAATGTTGGTTGGTGGCGGAATTTATTTACTTGGGATCTTCAGGTTTTAATGTATTAAAGGACGTGCGGGttgcaggta                                               >  1:1441184/1‑143 (MQ=9)
                                                                                                       tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAACCAGGGGATATCAAAAAAAGTTTGATTCATGTGGGTAGGTGGCGGAATTGGTAGACGCGCGATCGTCAGGTGTTAGTGTACTTACGGCCGggggggggggaggcc                                               >  7:1637711/1‑141 (MQ=21)
                                                                                                        gtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAACAGGGGATATCAAAAAAAGTTAGATGACTGTTCTATGGTGCCGGAATTGTTATAAGGGGGATCATCAGGTGTTAATGTAATTAAGGAAGTGGGGGTTGAAggaaa                                              >  1:1670082/1‑146 (MQ=16)
                                                                                                         ttCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGAATGAGGGAAGCTGGCGGAATTGGTTGAAGCGAGAGCTTAAGGGGTTAGTGTCATTAGGCACGTGGGGATTCAAGTcacc                                             >  2:3578/1‑150 (MQ=25)
                                                                                                         ttCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGAGTTCAAAAAAAGTAAGATGCGTGTTGGTAGGTGCCGGAATTGGTTGACGCGGTAGCTTCAGGTGTTAGTGTAGTTAGGGCCGTGGGGGTTGAAGTcacac                                            >  2:14930/1‑147 (MQ=25)
                                                                                                          tCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGAGATCAAAAAAAGTAAGATGAGTGTTCGATCGTGGCGGAATTTTTTGCCGGGGAAGCTTCATGTGTTAGTGTACATTAGGACGTGGGGGTTGAAGTaacccc                                           >  1:1006811/1‑151 (MQ=19)
                                                                                                           cTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCTAGATCAAAAAAAGTTAGATGCATGTTGTTTGCTGGCGGAATTGCTTTAATTGGTATCTTCATGTTTTACTGTAAATAAGGACGTGGGGGTTGAAGtaacaacc                                          >  1:927907/1‑144 (MQ=9)
                                                                                                            ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGTTCAAAAAATGTAAGATGAATGTGGTATGGTGGCGGAATTGGAATACGAGATAGCTTCAGGTTTTAGTGTACTTAAGGAAGTGGGGGTTGAAggaaaacccc                                         >  5:1942188/1‑142 (MQ=19)
                                                                                                                  aCGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAAAGTATGATGCCTGTGCGAAGGTGGCGGAATTGGTAGACGGGCTAGCTTCAGGTGTTAGTGTCGTTACGGCCGTGGGGGTTGAAGGacccccgcaggccc                                   >  5:1021321/1‑143 (MQ=21)
                                                                                                                   cGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTAAGATGAGTGTGGGAAGGTGGCGGAATTGGTAGACGGGGTAGCTGCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTTGAAGGCCCCCCCCTCGcccc                                  >  1:1069440/1‑151 (MQ=25)
                                                                                                                           ttCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGACTAGGTAGCTTCAGGTGTTACTGTAATTGCGGACGTCGGGGTGGAAGTCACCGCCCTCGCCCCAAATATCt                          >  3:1361873/1‑151 (MQ=21)
                                                                                                                           gccccccccccccccTCGCACTAAACCACGCCAAATCCAAAAAAGTACCATCAATTTGCGCAGGTGCCGGAATTGGTAGACGCGCGAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCt                          <  7:871285/144‑1 (MQ=14)
                                                                                                                              ccgTCCCCCCCCGCGCACCAACCCAGGCGATATCAAAAATATTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGGTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTa                       <  7:890243/149‑1 (MQ=18)
                                                                                                                               aGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAATGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGAGTTAGTCTCCTTACGGACGTGGGGGTTCAAGTCCCCACCCAGGCACCAAatatatgtt                       >  2:398508/1‑145 (MQ=21)
                                                                                                                                gTCCCCCCCCTCGCACCAAACGAGGCTATATCAAAAAAAGTAAGATGACTGTGCTAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTACCCCCCCTCGCCCCAATTATCTTTTCt                     >  1:425797/1‑151 (MQ=255)
                                                                                                                                gTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGCGGCGCTAGCGGCAGGTGTTAGTGTCCTTACGGAAGTGGGGGGTGCCGTCCCCCCCCCCGCGCCaagttttgttcat                     >  7:1053138/1‑140 (MQ=21)
                                                                                                                                  tcccccccTCGCACCAAACCAGGCGATATCAAAAAAAGTAACATGACTGAGCGAAGGTGGCGGAATTGGTAGACGAGCAAGCATCAGGAGTTAGTGACCTAACGCACGTGCGGGTTCAACTAACACCCCACGCACCAAatatatataatt                    >  8:1588502/2‑142 (MQ=16)
                                                                                                                                   cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAATTTTGTTTACTTcc                  >  3:362656/1‑151 (MQ=255)
                                                                                                                                    ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCt                 >  5:1493003/1‑151 (MQ=255)
                                                                                                                                     cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                >  5:2171042/1‑151 (MQ=255)
                                                                                                                                     cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                >  5:743791/1‑151 (MQ=255)
                                                                                                                                     cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                >  4:477836/1‑151 (MQ=255)
                                                                                                                                      ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGTCCCCCCCCTCGCACCACTTATCTTTACTTCCgtt               >  3:228334/1‑151 (MQ=255)
                                                                                                                                      ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCGCGCACCAAATATCTTTACTTACttt               >  7:1749216/1‑151 (MQ=255)
                                                                                                                                       cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCGTTACGGACGTGGGCCATGAACACCCCCCCCTCGCACCAATTATCTTTACTTCCtttg              >  7:2230043/1‑150 (MQ=25)
                                                                                                                                         cTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCtt            >  3:595624/1‑151 (MQ=255)
                                                                                                                                          tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTGCTTTCTTg           >  4:2047639/1‑151 (MQ=255)
                                                                                                                                           cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCGCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGt          >  7:1687933/1‑151 (MQ=255)
                                                                                                                                           cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGt          >  3:535240/1‑151 (MQ=255)
                                                                                                                                            gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATATTTACTTCCTTTCTTGtt         >  5:1723247/1‑151 (MQ=255)
                                                                                                                                             cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCGTTTCTTGttt        >  6:2085411/1‑151 (MQ=255)
                                                                                                                                             cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt        >  2:1781720/1‑151 (MQ=255)
                                                                                                                                             cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt        >  4:985926/1‑151 (MQ=255)
                                                                                                                                             cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCACCATATATCTTTTCTTCCGTTCATGttt        >  2:379531/1‑151 (MQ=21)
                                                                                                                                              aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtttg       >  5:1511533/1‑150 (MQ=255)
                                                                                                                                               ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct      >  3:105396/1‑151 (MQ=255)
                                                                                                                                               ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct      >  4:1369475/1‑151 (MQ=255)
                                                                                                                                                cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTTCCAGTCCCCCCCCGCGCACCAATTATCGTTACTTCCTTTCTTTTttctt     >  5:216213/1‑151 (MQ=255)
                                                                                                                                                cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTATtgtttgtt     >  2:2120773/1‑151 (MQ=255)
                                                                                                                                                cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttctt     >  2:45217/1‑151 (MQ=255)
                                                                                                                                                 aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttg    >  8:2120122/1‑150 (MQ=255)
                                                                                                                                                  aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATCTTTACTTCCGTTCTTGTttcttgc   >  4:347963/1‑149 (MQ=255)
                                                                                                                                                  aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTGGTGTttcttgc   >  2:1545995/1‑149 (MQ=255)
                                                                                                                                                   aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCt  >  4:683643/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCT  >  DH1/3906684‑3906981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑