Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8370CG26.7% 190.5 / 23.7 114intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (56/21);  new base G (27/1);  total (91/23)
Fisher's exact test for biased strand distribution p-value = 6.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906691‑3906987
                                                                                                                                                  |                                                                                                                                                      
ggtCAAGTCCCCCCCCTCGCAGCAAAAACCACGTTGATATTGCTCGCCCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAAACGAGGCGata                                                                                                                                                    <  7:114504/149‑1 (MQ=255)
 ccccacgccccccccTCCCACCAACCACCACGTTGAAAGACCTCCCCCTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                                   <  8:1892881/144‑1 (MQ=17)
   catgTCCGCCCCCTCGGACCAATAACGTCGTTGATTTTGTTGTGTGTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCGCCCCTCGCACCAAAGGAGGCGATATc                                                                                                                                                  <  8:1666132/147‑1 (MQ=14)
          cccgccTCGCACCAAAAACCTCGTTGTTATAGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAg                                                                                                                                          <  7:502003/151‑1 (MQ=255)
           cccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCATCAGGTGATAGTGTCCTTAAGGACGTGGGGGTGGCAATCCCCCCCCACGCCACAAAACAGAGGAGATaaaaaaaac                                                                                                                                          >  1:762156/1‑149 (MQ=18)
            cgccTCGCTCCAACCAACTCGTTTAAATTGCTGCCTCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTa                                                                                                                                        <  3:298011/149‑1 (MQ=17)
             cccTCCCACCAACAAACTCGTTTAAATACCTCCCCGTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTaa                                                                                                                                       <  5:612783/151‑1 (MQ=17)
                tCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTAAAATCCCCCCCCACCCCCCAAAAAACGCAATATaaaaaaaaattaaa                                                                                                                                     >  8:2052724/1‑144 (MQ=18)
                   cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGTATAAATCATAAAAAGTAAGAtca                                                                                                                                  >  4:2110510/1‑148 (MQ=18)
                   cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAACGAGGCGAAATCAAAAAAAGTAAGATGa                                                                                                                                  >  2:2119422/1‑150 (MQ=255)
                    aCCAAAAACCTCGTTGATATTGCTCGCACTGGGTGAAGGTTGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACt                                                                                                                                <  4:763241/151‑1 (MQ=255)
                    aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAAGAACGGAAGAGCAAACAAAAAAAGTacactcacg                                                                                                                                >  3:1967964/1‑143 (MQ=17)
                     ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTCTAGTCCCCCCCCACGCCCCAAACCAGGGGAAATCaaaaaaatttatttggctt                                                                                                                               >  7:2017071/1‑139 (MQ=18)
                     cataaaaCCACGTTGATATTGCTCGCTCTGGGTTAGTGTGGGGTATTTCGACGTCTCGGCTGCGGCTGTTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtg                                                                                                                               <  7:1221168/148‑1 (MQ=16)
                      cAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAACGAGAAATCAAAAAAAGTTAGATgcatg                                                                                                                               >  8:1963371/1‑146 (MQ=18)
                       aaaaaCCTCTTTGATATTGCTGTCAGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                             <  8:1678408/151‑1 (MQ=21)
                        agaactcgtttGATATTGCTCGAAGTGTGCGATTGGGTTGTAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGc                                                                                                                            <  1:1994458/142‑1 (MQ=18)
                        aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCCCACCAAATAAGGCTATATCAAAAAAAGttaatttcatgtct                                                                                                                            >  7:929867/1‑138 (MQ=18)
                          aaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAAAGGGTAAATAAATAAATGTAAGAttaatgatat                                                                                                                           >  6:1570322/1‑141 (MQ=17)
                          aaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACTATAAATCAAAAAAAGttacatcaatgatttt                                                                                                                          >  2:162568/1‑136 (MQ=17)
                             cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGAGTTCAAAAAATGTTTGGTGACtgtggtttgt                                                                                                                       >  5:54277/1‑145 (MQ=18)
                             cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAATTAGTCGGTATCACACAACTGAAGAAGACTGTGcccact                                                                                                                       >  5:2038579/1‑146 (MQ=17)
                             atcGTTTATATTACTGGCTGTTGGGGCAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAgg                                                                                                                       <  5:507275/149‑1 (MQ=18)
                              aagTTGATATTTCTCTCTTTCGGTTAAGGAGGCTGTTTTGTTATACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGt                                                                                                                      <  6:884007/149‑1 (MQ=18)
                              aCGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAACTCCCCCCCCTCCCACCAAAAAACGTTAAATCCAAAAAAATTAGATGaaagagaaatact                                                                                                                      >  4:1060059/1‑139 (MQ=14)
                                     aatacctcccgctctgCGAAGGTGGCGGAATTTTTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTTGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAATATTACTGTGCGAAGGTGGCGGaa                                                                                                               <  2:2295266/136‑1 (MQ=18)
                                           tCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCTAGTTCAAATAAAGTTAGATGACTGTGGGTTGGTGGCGGATTTGGtt                                                                                                         >  7:1251225/1‑150 (MQ=18)
                                           tCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCCGCCCCAAAAGCGGGGATAGCAAAAAAATTTAGTTGAGTGTTGGTAGGTCGCGGGAATGGtt                                                                                                         >  7:1612310/1‑150 (MQ=14)
                                              ccctcgtccgatctGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTATGGTTTTTTTGTTTCTAGTCCCCCCCCTCGCACCAAACGAGTAGATATCAAAAAAATTAAGATGACTGTTCGAAGGTGGCGGAATTGGTAGAc                                                                                                      <  4:1381574/138‑1 (MQ=21)
                                               tttcagCGAAGGGGGCGGAATTGTTATACGATGTAGGTTCAGGTGTTCCTCCGCTTCCTCTCGCGCGCTTGAAACTCCCCCCACTCGGCCCAACCGCTCCGATATCAATAAAAGTAATCTGTGTGTGCGAAGGTGGCGGAATTGGTAGAc                                                                                                      <  1:1831408/145‑1 (MQ=255)
                                                cTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGTTAGATAAATAAAAGTTTGATGACTGTTGTTTGTTGGCGGAATTggattaatt                                                                                                    >  7:171229/1‑144 (MQ=14)
                                                              cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGTAGATCAATAAAAGTTAGATGACTGTTGTTAGCTGGCGGAATTGCTATAATCTGTAGCTTCatgat                                                                                      >  5:1120830/1‑147 (MQ=14)
                                                               ggAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTAATTCAAATAATGTTTGTTGCATGTGTTTAGGTGGCGGATTTTGTTTAATTGTTAGCTTCAGgtgt                                                                                     >  5:28750/1‑151 (MQ=2)
                                                                  aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAAAGTATGATGCCTGTGCTAAGGTGGCGGAATTGGTAGACGGGGTAGCTGCAGGTGtt                                                                                    >  5:1425655/1‑149 (MQ=21)
                                                                       tAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGTAATTAAATAAATGTTAGATGAGTGTTTGTAGGTGGCGGATTTGCTATACTAGATAGCATCATGGGTTacgcta                                                                              >  4:2232750/1‑145 (MQ=2)
                                                                            ccgcTACCTGCAGGCGTGAGTGGTCTTACGGCCGTGGGGCGTCATGTTGCATTTCTCGCCCTAATCGATCCGATTTCAAATAAAGTTTGATGACTGTGCGAAGGTGGCTGAATTGGTTTACGCGCTAGCTTCAGGTGTTAGTGTCCTTACg                                                                        <  8:1164076/150‑1 (MQ=9)
                                                                              gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAAAAGAGTGGATATCAAAAAAAGTTTGTTGACTGTTGTTTGGTGGCGGAATTGGTTTACGCTGGAGCATCAGGTTTTActgaaaatagggc                                                                      >  7:394471/1‑141 (MQ=9)
                                                                               cTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCACGCCCCAAAACAGGGGATTTCAAAAAAAGTTTTATGCCTGTTGTTTGGTGGCGGAATTGGGAGACGCGCAAGGGTGACGTGGTATTGTccattaggac                                                                     >  7:2000101/1‑143 (MQ=9)
                                                                                tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCCCACCAAAAGAGGCGATATAAAAAAAATTATTATGACTGTTGGAATGTTGCGGAATTTTTATAACCGGGATCGTCAGGTGTTAGGGTACTTACggccc                                                                    >  7:831349/1‑148 (MQ=12)
                                                                                  gCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGTGGGGAGTTCAAAAAAAGTTTGATGAGTGTGGTTAGGTGGCGGATTGAGAATAAGTGATATCATCAGGTGTTAgtctaaaaacggcagtg                                                                  >  1:167228/1‑136 (MQ=9)
                                                                                   cTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTTGTTGAATGTTTTTAGGTGGCGGAATTGTTATAAGTGATATCATCATGTGTTActgtaaattaggccgtgg                                                                 >  7:1227668/1‑137 (MQ=12)
                                                                                       aGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCACCAAAGGAGGGGATTTCAAAAAAAGTTAGTTGAATGTTGTTTGGTGGCGGAATTTGTATACGAGTTAGCATCAGGTTTTAGTGTAATTAAGGACGTGGGGGt                                                             >  7:1160540/1‑151 (MQ=16)
                                                                                          tgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAAATCATAAAATGTTAGTTGAATGTTCTTAGGTGGCGGAATATGTATACTGGGGATCTTCAGGTGGTAGTGTCAATACGGCCGGGGGGGTTc                                                           >  5:1093937/1‑150 (MQ=12)
                                                                                           gtTAGTGTTCTTACGGACGTGGGGGTTCAACTACCCCCCCACCCCCCAAAAGAGGCGATATCAAAAAAAGTAAAATGAATGTTCGTAGGTGGCGGAATTTGAATACGAGCGATCATCAAGTGTTATTGTACTTAAGGACGAGGGGGtgga                                                          >  8:116431/1‑147 (MQ=255)
                                                                                             tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGAAGGGGATATCAAAAAAAGTTAGATGAATGTTGTAAGGTGCCGGATTTGTTATACGCGATAGAATCAGGTTTTAGTGTAAAATAGGACGTGGGGGttgtagg                                                       >  2:1069189/1‑146 (MQ=16)
                                                                                             tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTTAGATGAGTGTTGTATGGTGGCGGAATTGGTAGAAGAGATAGCAGCAGGATTAAGTGTAAATAAGGCCGTGGGGGtggaagg                                                       >  2:1058495/1‑145 (MQ=16)
                                                                                             tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGACGGTATATCAAAAAATGTAAGATCACTGTGAGATCGTGGCGGAATTGATAGAAGGAATAGCTTCAGGATGAAATGTCATTAAGGACGTGGGGCTTCa                                                          >  2:782379/1‑148 (MQ=16)
                                                                                                tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCCAAAAATTTTAGTTGAATGTTGGTTGGTGGCGGAATTTATTTACTTGGGATCTTCAGGTTTTAATGTATTAAAGGACGTGCGGGttgcaggta                                                     >  1:1441184/1‑143 (MQ=9)
                                                                                                tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAACCAGGGGATATCAAAAAAAGTTTGATTCATGTGGGTAGGTGGCGGAATTGGTAGACGCGCGATCGTCAGGTGTTAGTGTACTTACGGCCGggggggggggaggcc                                                     >  7:1637711/1‑141 (MQ=21)
                                                                                                 gtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAACAGGGGATATCAAAAAAAGTTAGATGACTGTTCTATGGTGCCGGAATTGTTATAAGGGGGATCATCAGGTGTTAATGTAATTAAGGAAGTGGGGGTTGAAggaaa                                                    >  1:1670082/1‑146 (MQ=16)
                                                                                                  ttCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGAGTTCAAAAAAAGTAAGATGCGTGTTGGTAGGTGCCGGAATTGGTTGACGCGGTAGCTTCAGGTGTTAGTGTAGTTAGGGCCGTGGGGGTTGAAGTcacac                                                  >  2:14930/1‑147 (MQ=25)
                                                                                                   tCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGAGATCAAAAAAAGTAAGATGAGTGTTCGATCGTGGCGGAATTTTTTGCCGGGGAAGCTTCATGTGTTAGTGTACATTAGGACGTGGGGGTTGAAGTaacccc                                                 >  1:1006811/1‑151 (MQ=19)
                                                                                                    cTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCTAGATCAAAAAAAGTTAGATGCATGTTGTTTGCTGGCGGAATTGCTTTAATTGGTATCTTCATGTTTTACTGTAAATAAGGACGTGGGGGTTGAAGtaacaacc                                                >  1:927907/1‑144 (MQ=9)
                                                                                                     ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGCGATATCAAAAAAAGTTAGATGCCTGTGGGTTGCTGGCGGAATTTGTTTAAGGGGTAGCGTCAGGTTTTACTGTAATAACGGCAGTGGGGGGTCCCGTgccccccc                                               >  3:1041359/1‑151 (MQ=16)
                                                                                                     ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGTTCAAAAAATGTAAGATGAATGTGGTATGGTGGCGGAATTGGAATACGAGATAGCTTCAGGTTTTAGTGTACTTAAGGAAGTGGGGGTTGAAggaaaacccc                                               >  5:1942188/1‑142 (MQ=19)
                                                                                                           aCGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAAAGTATGATGCCTGTGCGAAGGTGGCGGAATTGGTAGACGGGCTAGCTTCAGGTGTTAGTGTCGTTACGGCCGTGGGGGTTGAAGGacccccgcaggccc                                         >  5:1021321/1‑143 (MQ=21)
                                                                                                            cGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTAAGATGAGTGTGGGAAGGTGGCGGAATTGGTAGACGGGGTAGCTGCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTTGAAGGCCCCCCCCTCGcccc                                        >  1:1069440/1‑151 (MQ=25)
                                                                                                             gTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTAGGGACGTGGGGGTTGAAGGACCCCCCCTGGCAcc                                        >  7:440855/1‑150 (MQ=255)
                                                                                                                    ttCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGACTAGGTAGCTTCAGGTGTTACTGTAATTGCGGACGTCGGGGTGGAAGTCACCGCCCTCGCCCCAAATATCt                                >  3:1361873/1‑151 (MQ=21)
                                                                                                                    gccccccccccccccTCGCACTAAACCACGCCAAATCCAAAAAAGTACCATCAATTTGCGCAGGTGCCGGAATTGGTAGACGCGCGAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCt                                <  7:871285/144‑1 (MQ=14)
                                                                                                                     gcccgcccccccccGCCCACCACACGAGCCGATATCATTTATATTTATAGGCCTCGGCGAAGGTGGCTGAATTGGTACTAGCGTTATCTTCAGGTGTTAGTGTTTTTTAGGTCGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCtt                               <  4:230396/145‑1 (MQ=2)
                                                                                                                      acatacccccaccTCGCACAAAAACAATGGATATCAAAAAATTTACGATCACTTTGCTATTGGGTTGTAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCttt                              <  8:1217244/146‑1 (MQ=9)
                                                                                                                       ccgTCCCCCCCCGCGCACCAACCCAGGCGATATCAAAAATATTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGGTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTa                             <  7:890243/149‑1 (MQ=18)
                                                                                                                        aGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAATGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGAGTTAGTCTCCTTACGGACGTGGGGGTTCAAGTCCCCACCCAGGCACCAAatatatgtt                             >  2:398508/1‑145 (MQ=21)
                                                                                                                         gTCCCCCCCCTCGCACCAAACGAGGCTATATCAAAAAAAGTAAGATGACTGTGCTAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTACCCCCCCTCGCCCCAATTATCTTTTCt                           >  1:425797/1‑151 (MQ=255)
                                                                                                                         gTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGCGGCGCTAGCGGCAGGTGTTAGTGTCCTTACGGAAGTGGGGGGTGCCGTCCCCCCCCCCGCGCCaagttttgttcat                           >  7:1053138/1‑140 (MQ=21)
                                                                                                                           tcccccccTCGCACCAAACCAGGCGATATCAAAAAAAGTAACATGACTGAGCGAAGGTGGCGGAATTGGTAGACGAGCAAGCATCAGGAGTTAGTGACCTAACGCACGTGCGGGTTCAACTAACACCCCACGCACCAAatatatataatt                          >  8:1588502/2‑142 (MQ=16)
                                                                                                                            cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTcc                        >  7:1359611/1‑151 (MQ=255)
                                                                                                                            cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAATTTTGTTTACTTcc                        >  3:362656/1‑151 (MQ=255)
                                                                                                                             ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCt                       >  5:1493003/1‑151 (MQ=255)
                                                                                                                              cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  5:2171042/1‑151 (MQ=255)
                                                                                                                              cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  4:477836/1‑151 (MQ=255)
                                                                                                                              cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  5:743791/1‑151 (MQ=255)
                                                                                                                               ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGTCCCCCCCCTCGCACCACTTATCTTTACTTCCgtt                     >  3:228334/1‑151 (MQ=255)
                                                                                                                                cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCGTTACGGACGTGGGCCATGAACACCCCCCCCTCGCACCAATTATCTTTACTTCCtttg                    >  7:2230043/1‑150 (MQ=25)
                                                                                                                                cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTc                    >  6:2146193/1‑151 (MQ=255)
                                                                                                                                  cTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCtt                  >  3:595624/1‑151 (MQ=255)
                                                                                                                                   tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTGCTTTCTTg                 >  4:2047639/1‑151 (MQ=255)
                                                                                                                                    cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCGCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGt                >  7:1687933/1‑151 (MQ=255)
                                                                                                                                    cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGt                >  3:535240/1‑151 (MQ=255)
                                                                                                                                     gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATATTTACTTCCTTTCTTGtt               >  5:1723247/1‑151 (MQ=255)
                                                                                                                                      cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt              >  4:985926/1‑151 (MQ=255)
                                                                                                                                      cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt              >  2:1781720/1‑151 (MQ=255)
                                                                                                                                      cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCACCATATATCTTTTCTTCCGTTCATGttt              >  2:379531/1‑151 (MQ=21)
                                                                                                                                       aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtttg             >  5:1511533/1‑150 (MQ=255)
                                                                                                                                        ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct            >  4:1369475/1‑151 (MQ=255)
                                                                                                                                        ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct            >  3:105396/1‑151 (MQ=255)
                                                                                                                                         cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTTCCAGTCCCCCCCCGCGCACCAATTATCGTTACTTCCTTTCTTTTttctt           >  5:216213/1‑151 (MQ=255)
                                                                                                                                         cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTATtgtttgtt           >  2:2120773/1‑151 (MQ=255)
                                                                                                                                         cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttctt           >  2:45217/1‑151 (MQ=255)
                                                                                                                                          aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGTCCCCCCCCACGCCCCAATTATCTTTACTTCCTTTCTTGTttgttc          >  3:598477/1‑151 (MQ=255)
                                                                                                                                          aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCGTTCTTGTttcttc          >  1:538902/1‑151 (MQ=255)
                                                                                                                                           aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATCTTTACTTCCGTTCTTGTttcttgc         >  4:347963/1‑149 (MQ=255)
                                                                                                                                            aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCt        >  5:272038/1‑151 (MQ=255)
                                                                                                                                            aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCt        >  4:683643/1‑151 (MQ=255)
                                                                                                                                              gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTAATTCCTTTCTTGTTTATTCCTTg      >  2:2000895/1‑151 (MQ=255)
                                                                                                                                               aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATATTTACTTGCTTTCTTGTTTGTTCCTTg      >  6:1650742/1‑150 (MQ=255)
                                                                                                                                                ggCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTGCTTTCTTGTTTCTTCCTTGAt    >  4:1422761/1‑151 (MQ=255)
                                                                                                                                                ggCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCCAATTTCTTTTCTTGCGTTGTTGTTTGTTTCttttt    >  7:1930424/1‑148 (MQ=17)
                                                                                                                                                  cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCGCGCACCAATTATCTTTTCTTCGTTTGGTGTTTGTTCCGTGAttt  >  5:502486/1‑151 (MQ=18)
                                                                                                                                                  |                                                                                                                                                      
GTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906691‑3906987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑