Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA27.4% 414.7 / 35.0 338intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (136/60);  new base A (72/2);  total (272/66)
Fisher's exact test for biased strand distribution p-value = 7.81e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

CTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121977‑4122272
                                                                                                                                                    |                                                                                                                                                    
ccaCCAACTTCGCTATACCCGCTGGGTGGGTCTGTCCATTTAAGCACTTTCACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCttt                                                                                                                                                   <  8:1973249/149‑1 (MQ=14)
   acAACTCAGCTTTTCGCTGGTGGTTGGTCTGTTGATTACATCTATTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTgca                                                                                                                                                <  6:1922535/150‑1 (MQ=14)
          aGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGACCGGGAAACGAGACTCGAACTCGCGACCCCCACCTTGGCAATGTCGTGCTCTACCAAATGAGCAATTACCGCTTGGGACGTCTG‑TTATTTGGAGGAAttt                                                                                                                                         >  6:1571684/1‑151 (MQ=14)
             tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCCACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTC‑TGCTTTGCAGGACTTTTc                                                                                                                                       >  2:1120444/1‑150 (MQ=255)
              aatcccggggggggggTCTGGGCATTAAATCCCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTc                                                                                                                                       <  1:1072217/139‑1 (MQ=14)
              aTTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGGTGGTCTG‑TTATGTGGAGCACTTTTCa                                                                                                                                      >  3:1642493/1‑150 (MQ=14)
                 cccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGTTTTGGAGCACTTTTCAAAtt                                                                                                                                  >  5:993809/1‑151 (MQ=255)
                 cccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTATG‑TTATTTTAAGCACTTTTCAAAtt                                                                                                                                  >  8:86593/1‑151 (MQ=17)
                 cccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TTTTTTGGAGGAATTTTCACAtt                                                                                                                                  >  1:602746/1‑151 (MQ=14)
                 cccGCGTGGGGGGTCTGTGACTTACAGCCCTTTCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAAtt                                                                                                                                  <  1:1496831/151‑1 (MQ=255)
                    gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGAGGTCTG‑TGATTTGGAGCACTTTTCAAATTTTg                                                                                                                               >  7:1640622/1‑151 (MQ=255)
                    gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATG‑TGTTGTGCAGGAATTTTCAAAttttt                                                                                                                               >  1:1217748/1‑150 (MQ=17)
                    gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGACCTATTCCCGCGTAGGTGGTCTG‑TGTTGTTAAGAAATTTTCAAAttttt                                                                                                                               >  6:2229571/1‑150 (MQ=14)
                      ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTGCAGCGTGGGTGGTCTG‑TGCTGTGGATCCCTTTTCAAATTTTgg                                                                                                                              >  5:131713/1‑150 (MQ=17)
                      ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTGCGCTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                              >  7:540819/1‑150 (MQ=255)
                       tGGGTGGTGTGTGTTTTTTAGGTCTTTCAAATTTGGGTGGGGGAAAGGAGACTGGAAGTCGCGACCCCGAGCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGa                                                                                                                             <  8:514089/150‑1 (MQ=14)
                       tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTT‑TTGTGTTCAGGAATTTTCCAATTTTggcg                                                                                                                            >  5:1898566/1‑149 (MQ=17)
                       gggtgggtCTGTCCCTTACATCACTTGCACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                              <  3:1365311/144‑1 (MQ=17)
                        gggTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TTGTGTGGCGCAATTTTCAAATTTTggcgg                                                                                                                           >  4:1183437/1‑148 (MQ=17)
                        gggTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGTGTGAAGCACTTTTCGAATTTTGgcgc                                                                                                                           >  4:779434/1‑151 (MQ=255)
                           gggtgtggcacTTACAGCACTTTCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCggg                                                                                                                        <  7:247145/141‑1 (MQ=255)
                              tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TTTGTTGCAGCACTTTTCTAATTTTGGAGCgggta                                                                                                                      >  3:96897/1‑148 (MQ=17)
                               ttgttcattatatcACTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGaaa                                                                                                                     <  8:1663871/137‑1 (MQ=14)
                                 gtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTAGGTGGTAAG‑TTATTTGAAGCAATTTTCAAATTTTTGAGCGGGAAACg                                                                                                                   >  8:915831/1‑150 (MQ=17)
                                   gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTAAT‑TTATTATCAGAACATTTCAAATATTGAAGCCAGAAACg                                                                                                                   >  5:1746609/1‑148 (MQ=14)
                                   gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCCGACTTCTG‑TTATATGAAGAACTTTTCAAATTTTGGAGAGGGAAACg                                                                                                                   >  2:595707/1‑148 (MQ=17)
                                     attacatcttgttaaaaTTTTGGATCCGCAACCGAGCCTGTAACTCCCTACCCCGACCTTTGCAAGGTAGTGCTCTAACAACTGAGCTATACCCGCTTGGGTGGTCTT‑TGCTTTTCATCACTTTTCAAATTTTGGAGCGGGAAACGAGACt                                                                                                              <  7:1073305/137‑1 (MQ=9)
                                      ttACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGACGTCTGTTGGATTAC‑GAACTTTTCTAATTTTTGAGCGGGAAACgatcgac                                                                                                             >  5:1139807/1‑146 (MQ=14)
                                       tACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TTATTTGGAGCACTTTTCAAATTTTGGAGCGGGAACGGAGACTcc                                                                                                            >  3:1851561/1‑150 (MQ=255)
                                        aCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTTCCGCACTTTTCAAATTTTCGAGCAGGACACGACAcgcg                                                                                                            >  5:267070/1‑150 (MQ=17)
                                          aGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTCTG‑TGCGTTTGAGGACTTTTCTAATTTTTGCGCGGGAACCgcgcggccaa                                                                                                          >  3:847742/1‑141 (MQ=14)
                                            cACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGTTATT‑TTGTTTTAAGCAATTTTCAAATTTAGGAGGCGTAAACGAGCCGCGaaaa                                                                                                        >  6:44547/1‑148 (MQ=14)
                                            cACTTTCAAATTTTGGACCGGGAAACGAGACTCGAACTCGCGACCCCGAACTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTACCGCGTGCGAGGTATG‑TTATATGAAGGACTTTTCAAATTTATGAGAGGTAAACGAGAcaccataa                                                                                                        >  8:1220871/1‑143 (MQ=14)
                                             aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGTTTGCAGCACTTTTCTAATTTTGGAGCGGGAAACGCGCCTCGAACTcc                                                                                                      >  7:1306961/1‑150 (MQ=255)
                                             aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATG‑TGATTTGCAGCAATTTTCAAATTTTGGAGGGGGAAACGAGCCTCGATCtac                                                                                                      >  7:959210/1‑149 (MQ=14)
                                              cTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCGTCTG‑TTATATGGAGCACTTTTCAAATTTTTGAGCGGGAAACGAGACTCGAACTcgc                                                                                                     >  8:613057/1‑151 (MQ=14)
                                              atataaaaTTTAGTTGCGCGAAACTCGCCTGGTACTCGCGAGCGCCACCTTGGCTATGTCGTTCTATAAAAACTTAGCTTTTCCCTCTTGGGTGGTGTG‑TTCCTTACAGTAC‑TTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTcgcg                                                                                                    <  5:810555/146‑1 (MQ=2)
                                               tttCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTG‑TTATGTTACGAAATTTTCAAATTTTTGAGAGGGAACCGGAACTCGAACtaacc                                                                                                    >  2:2279656/1‑147 (MQ=11)
                                                ttCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGGACTTTTCACATTTTGGAGCGGGAAACGAGCCTCGAACTcgcg                                                                                                    >  1:393181/1‑150 (MQ=255)
                                                   aaaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTAGGTCAG‑TGATATGAAGCACTTTTCAAATTTTGGAGAGCTAAACGAGACACCAAATAGCGAcac                                                                                                >  2:437452/1‑149 (MQ=14)
                                                   aaaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TGATTTGGAGGAATTTTCAAATTTTTGAGCGGTAAACGACCCTCCAACTACCGAccc                                                                                                >  5:2178968/1‑151 (MQ=14)
                                                     aTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTG‑TGATTTGAAGAACTTTTCAAATTTTGGCGAGGTAACCGCGAGTCGATATCGCGACACCg                                                                                              >  6:1250836/1‑151 (MQ=14)
                                                        ttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TTGTGTGGAGGAATTTTCAAATTTTTGAGGGGGAAACGAGACTGGAACTAGCGACACCGAcg                                                                                           >  1:958149/1‑150 (MQ=14)
                                                         tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTATT‑TGAGGTTGAGCAATTTTCAAATTTTTGAGCGGTAAAGGCGCGTCGAACTAGCGAcccctcggt                                                                                          >  1:522449/1‑146 (MQ=11)
                                                            cgccccaaaGGAGCCTGGACCTCCCGCCCCCTACCTGCGCAAGGGCGAGCCCAACAAACCCAGCTATACCCGCTGGGGTGGGGGG‑TGCTTTTCAGCAGTGTTAAAATTTTGGAGCGGCAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                       <  7:19892/145‑1 (MQ=2)
                                                            aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTgg                                                                                       >  7:658219/1‑151 (MQ=255)
                                                             gCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGTACGTCTG‑TGATATGAAGAACTTTTCAAATTTAGGAGCGGGAAACGAAACTCCAAATAGCGACCCCGACCtacc                                                                                       >  6:700721/1‑147 (MQ=14)
                                                              cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGc                                                                                      >  8:352716/1‑150 (MQ=38)
                                                              cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTG‑TGGTTTGCAGGACTTTTCAAATTTTTGAGCGGGTAACGAGCCTCGAACTCGCGACACCGCCCtggcc                                                                                      >  1:1643640/1‑146 (MQ=14)
                                                                   aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGACACGAACTCGCGACCCCGACCTTGGCAAGGTc                                                                                >  3:1010816/1‑151 (MQ=255)
                                                                   aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGAGTCGAACTAGCGACCACGACCTTGGCAAGGTc                                                                                >  3:776926/1‑151 (MQ=255)
                                                                      gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTTGAGCGGGAAACGAGACTCGAACTCGCGACCCCTACGTTGGCAAGGTCGTg                                                                             >  2:772406/1‑151 (MQ=255)
                                                                      gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGATGTCGCGACCCCGCCCTTGGCAAGGTCGTg                                                                             >  3:508222/1‑151 (MQ=255)
                                                                         aCTCGATCTCGCGACCCCGACCTTGGTAAGGTCGTGCTCTACAAACTGAGCTATTCCGGCTTGGGTGGTTGT‑TGCTTTTCTTCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct                                                                           <  4:375801/150‑1 (MQ=11)
                                                                         aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTGTGCCGCACTTTTCAAATTTTGGAGCGGGAACCGCGCCTCGAACTCGCGACCCCGACCTGGGCATGCTCGTGcgc                                                                          >  7:1173488/1‑149 (MQ=14)
                                                                         aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCGGTGCAGCAATTTTCAAATTTGGGCGGGGGAACCGCGACTCCAACACGCGACCCCGACGTGGGAATGCTCGGGctc                                                                          >  5:569957/1‑151 (MQ=11)
                                                                           tCGAACTCGCGACCCCGACCTTCGCAAGGTCCTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTATG‑TTCTGAGAAGCACTATTCAAATTTTTGAGCGGGAAACGACACACGAACTAGCGACCACGACCTAGGCAAGGTCGTCctct                                                                         >  8:1894722/1‑150 (MQ=11)
                                                                            cGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                       >  5:1473123/1‑151 (MQ=38)
                                                                             gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCAG‑TGCTATGCAGGACTTTTCAAATTTAGGAGAGGGAAACGAGACTCGATATCGCGACCACGAACTAGGCAAGGACGACCTCTAcc                                                                      >  6:1438192/1‑151 (MQ=14)
                                                                              aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGACGTAAT‑TTATATTAAGAAATTTTCAAATTTATGAACATCAAAAGAGACTCGAACTCGCGAACACGAACTTGGCAAGTTCGTGCTATAcc                                                                      >  8:1718500/1‑150 (MQ=2)
                                                                                cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGCGCCTCGAACTCGCGACACCGACCTTGGCAAGGTCGTGCGCTACGAAc                                                                   >  1:1708401/1‑151 (MQ=14)
                                                                                  cgcgCCCCGGTCTTTGTCAAGGTCTCGCGGTAACACGTGAGCTTATGCCGGGTGGGTCGTGTG‑TCCTTTGGAGCTCTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAc                                                                   <  3:675996/149‑1 (MQ=2)
                                                                                   gcgACCCCGACCTTGGCAAGGTCGTCCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGATTTGGAGCAATTTTCTAATTTAGGAGGCGGAAACGCCACACGAACTCTCGACACCGACGTGCCCAAGGAACACCTCTACCAACTg                                                                 >  1:859992/1‑150 (MQ=9)
                                                                                    cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTG‑TTATTTGACGGAATTTTCAAATTTTTGAGAGGGTAACGCGACTCGAACTAGCGACCCAGACCTAGGGAAGGTCGTGCTCTACCAACTg                                                                 >  2:935335/1‑149 (MQ=11)
                                                                                    cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTGGGTTGC‑GGACTTTT‑AAATTTTGGGGGGGGTTACGGGAGGCGCAGTAGCCCCCCCGTCGTTGGCTAGGGCCGGCGCCACCAACGGAgt                                                              >  5:158705/1‑150 (MQ=0)
                                                                                     gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTATG‑TGATTTTAAGGACTTTTCAAATTTTTGAGCGGGAAACGAGACTCGAAATAGCGACCCCGACATAGGGAAGGTCGTGCTCTACCAACGGAGc                                                              >  4:17620/1‑151 (MQ=11)
                                                                                     gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGTTTGCAGCACTTTTCTAATTTTGGAGCGGGAAACGAGGCTCGAACTCGCGACACCGACCTTGGCAAGGTCGTGCTCTACCAACTGAgg                                                              >  7:1799334/1‑150 (MQ=255)
                                                                                     gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGATTTGAAGAACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGATCTAGCGACCACGACCTAGGCAAGGTCCTGCGCTACAAACTGAGc                                                              >  6:927849/1‑151 (MQ=14)
                                                                                        cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTCGTCTG‑TGGTTTTCAGCAATTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAt                                                           >  5:409256/1‑151 (MQ=11)
                                                                                          cGACGTTGTCAAGGACGGGCTCTACCAACTCAGCTATTCCCCCTGGGGTGGGCTG‑TGCTTTCCTGCTCTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                         <  3:1908903/151‑1 (MQ=11)
                                                                                            aCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGATTTTAAGAAATTTTCAAATTTTGGAGAGGTAAACGAGACTCGAAATCGCGACCACTACATAGGAAAGCTAGAGCTCTAACAACTGAGATATTccc                                                       >  8:431951/1‑151 (MQ=11)
                                                                                               ttGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTG‑TGGTTTGCAGCACTTTTCAAATTTTTGAGAGGTAAACGAGCGTCGAACTAGCGACAACGACATTGGCAAGCTCGTGCACTACAAACTGAGCTATTCCcgcc                                                    >  1:620617/1‑150 (MQ=14)
                                                                                               ttGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAAATCGCGACCAAGAACTTGGCAAGGTCGTGCTCTACCAACTGAGATATTccctct                                                    >  7:170957/1‑148 (MQ=14)
                                                                                                    aaGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTCCTCTACCAACTGAGCTATTCCCGAGtact                                                >  3:310110/1‑150 (MQ=255)
                                                                                                       gTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGATTTGCAGAAATTTTCAAATTTTGGAGAGGGAAACGAGCCTCGATAACTCGACACCGACCTTCCCAAGGTCGACCTCAAAAAACTGATCTATACCAGCGTACTACtt                                            >  3:1064610/1‑151 (MQ=9)
                                                                                                            gCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTATG‑TGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAAATCGCGACCCCGACCTTGGCAAGGTCGTGCTCAAACAACTGAGATATTCCCGCGTACTACTTAAttt                                       >  6:546125/1‑151 (MQ=17)
                                                                                                             ctctACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGCGCGGGAACCGAGCCTCGAACTCGCGACCCCGAGCTTGGGAAGGTCCTGCTCTACCAACTGAGCTATTCCCGCGTAGTACTTAAtttt                                      >  2:1526456/1‑151 (MQ=17)
                                                                                                             cggttccAACTGAGATATTCCCTGTTGTTTGGTCTG‑TTCTTTTAATCAATTTTCAAATTTTGTAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAttt                                       <  8:132689/145‑1 (MQ=17)
                                                                                                               ctACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGc                                    >  1:218738/1‑151 (MQ=255)
                                                                                                                 aCCAACTGAGCTATTCCCGCTTGGGTGGTATG‑TGCTTTGCAGAAATTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGACATTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCtt                                  >  8:770900/1‑151 (MQ=255)
                                                                                                                    aaCTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTCGCGACCCCGACCTTGGGAAGGTCGTGCTCTACCAACTCAGCTATTCCCGCGGACTACTTAATTTTGCTTTCg                               >  4:2025273/1‑151 (MQ=255)
                                                                                                                    aaCTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  8:2060591/1‑151 (MQ=255)
                                                                                                                    aaCTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  7:2189057/1‑151 (MQ=255)
                                                                                                                       tGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTa                             >  3:1214345/1‑150 (MQ=255)
                                                                                                                               tCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  4:1356613/1‑150 (MQ=255)
                                                                                                                                cccGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTT‑GCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtt                  >  4:56996/1‑151 (MQ=255)
                                                                                                                                    cTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt               >  1:1588953/1‑151 (MQ=255)
                                                                                                                                    cTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt               >  2:1786779/1‑151 (MQ=255)
                                                                                                                                      tGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGc             >  5:1560016/1‑151 (MQ=255)
                                                                                                                                       gggTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcg            >  5:1999745/1‑151 (MQ=255)
                                                                                                                                        ggtggtTTT‑TGGTTTTCATGAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgt           <  4:1870586/151‑1 (MQ=18)
                                                                                                                                         gtggtCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtc          >  8:238866/1‑151 (MQ=255)
                                                                                                                                          ttgtCTT‑TGCTTTGAAGAAGTTTTTAATTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtc          <  2:214424/148‑1 (MQ=21)
                                                                                                                                           ggtCTG‑TGCTTTGCAGGACTTTAGAAATTATGTCGCGGCATACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcg         <  5:334815/150‑1 (MQ=21)
                                                                                                                                           ggtCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcgt        >  3:2268745/1‑151 (MQ=255)
                                                                                                                                                 gggCTTTCCAGCACTTTTCATATTTTGTCGCGGGAAACGAGCCGCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTAc     <  5:883393/147‑1 (MQ=25)
                                                                                                                                                  tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  6:417715/1‑151 (MQ=255)
                                                                                                                                                  tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  1:1836614/1‑151 (MQ=255)
                                                                                                                                                  tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  4:283344/1‑151 (MQ=255)
                                                                                                                                                   gCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  3:2133719/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121977‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑