Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 205,979 T→C D189D (GAT→GAC)  yhjG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1205,9790TC97.0% 2106.1 / 29.2 575D189D (GAT→GAC) yhjGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/8);  new base C (251/304);  total (262/313)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 576 total aligned reads displayed.

GACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGG  >  DH1/205832‑206125
                                                                                                                                                   |                                                                                                                                                  
 aCGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCt                                                                                                                                                >  1:1594914/1‑151 (MQ=255)
 aCGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCt                                                                                                                                                <  3:1004130/151‑1 (MQ=255)
   gTTTAATCTTGCCTAGGATGATAACAAAGACGCGAATCCAAAGCCGTCGGCATGCTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGAATTTTGTTGAGCCCTTa                                                                                                                                              <  2:1766830/151‑1 (MQ=255)
   gTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTa                                                                                                                                              <  6:2110970/151‑1 (MQ=255)
    tttAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCtt                                                                                                                                               >  4:325940/1‑149 (MQ=255)
    tttAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCtt                                                                                                                                               >  3:1818018/1‑149 (MQ=255)
     ttAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAgg                                                                                                                                            >  5:307421/1‑151 (MQ=255)
     ttAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAg                                                                                                                                             <  5:1264782/150‑1 (MQ=255)
       aaTCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGc                                                                                                                                           >  1:1944288/1‑150 (MQ=255)
            tGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGc                                                                                                                                       <  7:1321473/149‑1 (MQ=255)
             gCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCg                                                                                                                                     <  6:902460/150‑1 (MQ=255)
              ccAACGAAGAAAACAAACACGAGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGTGCGGATCTCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCt                                                                                                                                   <  4:900593/151‑1 (MQ=255)
               cAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTg                                                                                                                                  >  6:1367253/1‑151 (MQ=255)
                aaCGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGc                                                                                                                                 <  1:546530/151‑1 (MQ=255)
                       atAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTc                                                                                                                            >  3:1587671/1‑149 (MQ=255)
                         aaCAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCg                                                                                                                        >  3:1907416/1‑151 (MQ=255)
                          aCAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGa                                                                                                                       <  5:1615975/151‑1 (MQ=255)
                            aaaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGa                                                                                                                       >  8:1386490/1‑149 (MQ=255)
                            aaaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAg                                                                                                                     <  7:2213133/151‑1 (MQ=255)
                            aaaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGATGCCGTTCATCGaat                                                                                                                     >  1:996500/1‑150 (MQ=255)
                              aGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGtt                                                                                                                   >  4:994854/1‑151 (MQ=255)
                              aGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGtt                                                                                                                   >  6:638647/1‑151 (MQ=255)
                               gACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGtt                                                                                                                   >  2:330999/1‑150 (MQ=255)
                                aCGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGACATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTa                                                                                                                  >  8:271522/1‑150 (MQ=255)
                                 cgcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACt                                                                                                                <  2:120393/151‑1 (MQ=255)
                                   cgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTgg                                                                                                              <  7:1591059/151‑1 (MQ=255)
                                    gAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTgg                                                                                                              >  3:1365192/1‑150 (MQ=255)
                                     aaTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGAt                                                                                                            >  5:1093841/1‑151 (MQ=255)
                                     aaTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGAt                                                                                                            <  3:995308/151‑1 (MQ=255)
                                     aaTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGAt                                                                                                            >  5:478714/1‑151 (MQ=255)
                                      aTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATc                                                                                                           <  8:1299034/151‑1 (MQ=255)
                                       tGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCg                                                                                                          >  2:694091/1‑151 (MQ=255)
                                        gCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGa                                                                                                         <  2:1567410/151‑1 (MQ=255)
                                          aaaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGaaa                                                                                                       <  8:1906659/151‑1 (MQ=255)
                                          aaaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGaa                                                                                                        >  7:969285/1‑150 (MQ=255)
                                           aaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAg                                                                                                      <  3:1120187/151‑1 (MQ=255)
                                              ccGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGt                                                                                                    >  5:1863183/1‑150 (MQ=255)
                                                  cGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGc                                                                                               >  1:1001468/1‑151 (MQ=255)
                                                   ggCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCg                                                                                              <  8:1159701/151‑1 (MQ=255)
                                                   ggCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCg                                                                                              <  2:549111/151‑1 (MQ=255)
                                                    gCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                             <  3:716830/151‑1 (MQ=255)
                                                    gCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                             >  3:1465302/1‑151 (MQ=255)
                                                     cATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                             >  1:2306728/1‑150 (MQ=255)
                                                     cATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                             >  6:1081990/1‑150 (MQ=255)
                                                        ggTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATa                                                                                          <  5:992040/150‑1 (MQ=255)
                                                         gTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaaa                                                                                        <  6:1736711/151‑1 (MQ=255)
                                                           cGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                         >  4:1190439/1‑148 (MQ=255)
                                                           cGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAg                                                                                       <  4:173181/150‑1 (MQ=255)
                                                            gTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGa                                                                                      >  8:1794837/1‑150 (MQ=255)
                                                             tttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaa                                                                                     >  2:1989120/1‑150 (MQ=255)
                                                              ttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaaa                                                                                    >  8:756703/1‑150 (MQ=255)
                                                                cGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAg                                                                                  <  5:2247045/150‑1 (MQ=255)
                                                                  gCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTg                                                                               <  5:1763405/151‑1 (MQ=255)
                                                                   cTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTgg                                                                              >  5:1415088/1‑151 (MQ=255)
                                                                   cTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTgg                                                                              >  5:212632/1‑151 (MQ=255)
                                                                       ataataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCg                                                                           >  6:1464188/1‑150 (MQ=255)
                                                                         aataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAt                                                                         <  3:208771/150‑1 (MQ=255)
                                                                            atTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTAc                                                                      <  4:774280/150‑1 (MQ=255)
                                                                             ttCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGt                                                                    >  1:1837905/1‑151 (MQ=255)
                                                                              tCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtt                                                                   >  4:2244993/1‑151 (MQ=255)
                                                                                ttttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtttt                                                                 >  6:1931572/1‑151 (MQ=255)
                                                                                ttttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtttt                                                                 >  1:1234932/1‑151 (MQ=255)
                                                                                 tttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGttt                                                                  <  7:548933/149‑1 (MQ=255)
                                                                                   tCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTg                                                               <  4:1464817/150‑1 (MQ=255)
                                                                                    cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGc                                                             >  5:846753/1‑151 (MQ=255)
                                                                                    cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGAGGGCGATTACGTTTTTGGc                                                             >  5:202339/1‑151 (MQ=255)
                                                                                      aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                           >  2:1373085/1‑151 (MQ=255)
                                                                                      aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                           <  8:2138563/151‑1 (MQ=255)
                                                                                        cAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTg                                                          >  7:2067356/1‑150 (MQ=255)
                                                                                           gggCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAgg                                                      <  7:172731/151‑1 (MQ=255)
                                                                                            ggCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGgc                                                     <  3:647554/151‑1 (MQ=255)
                                                                                                gATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAg                                                 <  6:1885927/151‑1 (MQ=255)
                                                                                                   cGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAgggc                                              >  3:933980/1‑150 (MQ=255)
                                                                                                   cGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGa                                              <  3:475205/151‑1 (MQ=255)
                                                                                                    gCCATTTATGAAAAAGTAAGCAAAGCGGATGATGCTATTTTTGTTGACCCCGTACGCAAGGCGCTGCCGTTCAGGGTAGTTACTGGCTCGAAAGGTAAACCGGAAAAACAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGAc                                             <  3:838499/151‑1 (MQ=255)
                                                                                                    gCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGAc                                             <  2:25071/151‑1 (MQ=255)
                                                                                                    gCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGAc                                             >  3:2288531/1‑151 (MQ=255)
                                                                                                     ccATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACg                                            >  3:1289696/1‑151 (MQ=255)
                                                                                                             gaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAAc                                     <  7:1137435/150‑1 (MQ=255)
                                                                                                             gaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACg                                    >  6:341346/1‑151 (MQ=255)
                                                                                                              aCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACgg                                   >  1:733616/1‑151 (MQ=255)
                                                                                                                aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTg                                 >  4:1573483/1‑151 (MQ=255)
                                                                                                                aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTg                                 <  7:2011176/151‑1 (MQ=255)
                                                                                                                 aaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTg                                 >  2:861497/1‑150 (MQ=255)
                                                                                                                 aaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGa                                <  1:862042/151‑1 (MQ=255)
                                                                                                                   gTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAAc                              <  1:2181987/151‑1 (MQ=255)
                                                                                                                     aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAAcc                             <  7:707909/150‑1 (MQ=255)
                                                                                                                     aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAAcc                             <  8:589564/150‑1 (MQ=255)
                                                                                                                     aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCg                            <  4:1996547/151‑1 (MQ=255)
                                                                                                                        cAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                         >  6:121471/1‑151 (MQ=255)
                                                                                                                         aaaGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCa                        <  4:1311507/151‑1 (MQ=255)
                                                                                                                          aaGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCAc                       <  7:418444/151‑1 (MQ=255)
                                                                                                                           aGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACg                      <  6:2199433/151‑1 (MQ=255)
                                                                                                                             cGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACggg                    >  4:683240/1‑151 (MQ=255)
                                                                                                                               gATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGt                   >  1:1099795/1‑150 (MQ=255)
                                                                                                                                aTCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTAc                 >  8:165853/1‑151 (MQ=255)
                                                                                                                                      agaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaaa           <  5:716841/151‑1 (MQ=255)
                                                                                                                                       gaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaaa           >  6:377781/1‑150 (MQ=255)
                                                                                                                                          ttttGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAAt        <  4:482185/150‑1 (MQ=255)
                                                                                                                                              gttgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGc    <  2:2101538/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGG  >  DH1/205832‑206125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑