Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC23.6% 587.3 / 61.0 298G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (69/158);  new base C (68/2);  total (138/160)
Fisher's exact test for biased strand distribution p-value = 1.03e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447254‑3447553
                                                                                                                                                      |                                                                                                                                                      
 cACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCAcc                                                                                                                                                      >  4:750800/1‑151 (MQ=255)
    gATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCAccccc                                                                                                                                                   >  7:1515846/1‑149 (MQ=255)
          tCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCCCCGcccc                                                                                                                                             >  4:481309/1‑151 (MQ=255)
            aTGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCa                                                                                                                                           <  8:384767/151‑1 (MQ=255)
               gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCTGTGATCCCGGCAACGTGCTGCATATTATTGCGCGACCCCACCC‑CCTCCCCCa                                                                                                                                           >  6:32108/1‑148 (MQ=255)
                cggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCat                                                                                                                                       <  1:1057117/151‑1 (MQ=255)
                   cATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCGCCCCCAGCCTATg                                                                                                                                    >  2:338219/1‑151 (MQ=255)
                    aTCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCCCCGCCCCCCGCATATGc                                                                                                                                   >  5:504320/1‑151 (MQ=255)
                       ttCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGcacc                                                                                                                                >  3:1640902/1‑151 (MQ=255)
                         ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCCCCGccccccccattatcacccc                                                                                                                              >  2:1097237/1‑137 (MQ=255)
                          cacaTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGcaccac                                                                                                                              >  4:1484911/1‑150 (MQ=255)
                          cacaTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCCCCTCCCCCACCAAATGcacccc                                                                                                                              >  4:1332507/1‑148 (MQ=255)
                             aTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATcc                                                                                                                          <  4:505284/151‑1 (MQ=255)
                               cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCGACCCCAGCATATGccccacctacac                                                                                                                        >  2:905144/1‑145 (MQ=255)
                                ggCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGcac                                                                                                                                 <  8:1427293/141‑1 (MQ=255)
                                  ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCCCCACATccagccc                                                                                                                     >  7:418181/1‑150 (MQ=255)
                                  ccTGTAACCATTGCCCTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATccagccg                                                                                                                     <  4:380843/151‑1 (MQ=255)
                                     gTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGCACCACATccagccgccc                                                                                                                  >  3:1526099/1‑150 (MQ=255)
                                          cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATAGGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGGAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGt                                                                                                             <  2:1569740/151‑1 (MQ=255)
                                          cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGCCCCCCATccagccgcccgccgg                                                                                                             >  5:183202/1‑150 (MQ=255)
                                          cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCACCATATGCACCACATcccgccgccagccgg                                                                                                             >  4:968152/1‑150 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATTCACCACATCCAGCCCCCCGCCGTCCa                                                                                                          >  2:479805/1‑150 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCCAATCCAGCCGCCAGCCGTCCat                                                                                                         >  1:118126/1‑151 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCCCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTACGCTGATCCCGGCAACGTGCTGCAAATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCa                                                                                                          <  2:1527270/150‑1 (MQ=255)
                                               ccGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCCGCATATGCCCCACCTCCAGCCGCCAGCCGTGCat                                                                                                         >  2:694210/1‑150 (MQ=255)
                                               ccGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCCCCTCCCCCAGCATATTCACCCAAAACACCCGCCAGccatccat                                                                                                         >  5:1452676/1‑150 (MQ=255)
                                                cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                       <  7:1192639/151‑1 (MQ=255)
                                                   gCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTcc                                                                                                    <  4:1581314/151‑1 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACgg                                                                                                <  3:253967/151‑1 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACg                                                                                                 <  3:986064/150‑1 (MQ=255)
                                                        cccAAAATGTTTGAAGCCTATATACGGTTCCGGCTGTGTTTCTTTCGGCGCTTCGGTGATCCCGGCAACGTGGTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCCCATCCAGCCGCCAGCCGTCCATATTCGACGgc                                                                                               <  2:656417/150‑1 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                              <  3:939882/151‑1 (MQ=255)
                                                           aaaaTGTTCGCCGACAATTTACGCTTCCGGCTGGGTTTCTTTCGCCGCTGCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCt                                                                                            <  4:715453/151‑1 (MQ=255)
                                                            aaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCtt                                                                                           <  6:1373356/151‑1 (MQ=255)
                                                             aaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCttt                                                                                          >  2:1305551/1‑151 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCCGCATATGGACCACATCCACCCGCCCGCCGTCCATATTCCACGgcccgttgac                                                                                       >  7:181249/1‑143 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCa                                                                                    <  2:1039350/151‑1 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCCCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGcc                                                                                     <  7:972685/150‑1 (MQ=255)
                                                                      cGACAATATACGCTTCCGGCTGGGGTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTg                                                                                 <  2:7648/151‑1 (MQ=255)
                                                                          aaTATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCGCCCCCCCCCTATGCACCACATACCCCCGCCAGCCGTCCATATTCCCCGGCGCTTTCCGCCAGTGGCgg                                                                             >  1:935834/1‑151 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCCCCACATACAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCggcc                                                                           >  8:1261836/1‑149 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGCACCCCCTCCAGCCGCCCGCCCGCCATATTCCACGGCGCTTTCAGCCACTGGCggcc                                                                           >  4:827580/1‑148 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACa                                                                          <  5:1487998/151‑1 (MQ=255)
                                                                              taCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCCCCGCCCCCACCATATTCACCCCCACCACCCCCCACCCGTCCCAATTACACCGCGCGTTCAGCCAGTGGCgggcc                                                                          >  4:1634435/1‑147 (MQ=255)
                                                                               aCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCGCCCCCAGCATATTCCCCACATCCACCCGCCCGCCGGCCATATTCCACGGCGCGTTCAGCCAGTGGCGGCCaa                                                                         >  5:1027291/1‑150 (MQ=255)
                                                                               aCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCCCCTCCCCCAGCATATGCACCACATCCAGCCGCCACCCGTCCCTATTCCACGGCGCTTTCACCCAGTGGCGGACaa                                                                         >  3:1256147/1‑150 (MQ=255)
                                                                                cGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGGGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATa                                                                       <  1:1549318/151‑1 (MQ=255)
                                                                                 gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGGG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCGACGGCGCTTTGAGCCAGTGGCGGACAATAc                                                                      <  1:1466822/151‑1 (MQ=255)
                                                                                 gCTTCCGGCTGGGATTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAc                                                                      <  5:1648060/151‑1 (MQ=255)
                                                                                   ttCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCACCCGTACATATTACACGGCCCTTTCAGCCAGTGGCGGACAATAc                                                                      >  8:1092796/1‑149 (MQ=255)
                                                                                    tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGCCCCCCATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGCCAATACTgg                                                                   >  7:1071209/1‑150 (MQ=255)
                                                                                    tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACt                                                                     <  8:783776/149‑1 (MQ=255)
                                                                                    tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACt                                                                     <  3:1386490/149‑1 (MQ=255)
                                                                                     ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGt                                                                   <  3:1111701/150‑1 (MQ=255)
                                                                                        gCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCGCCCCCAGCATATGCACCACATCCAGCCGCCCGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTc                                                               >  1:473679/1‑151 (MQ=255)
                                                                                        gCTGGGGTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTc                                                               <  3:290448/151‑1 (MQ=255)
                                                                                         cTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCCCCTCCCCCCGCATATGCACCACATCCACCCGCCAGCCGTCCATATTACACGGCGCTTTAAGCCAGTGGCGGCCCATACTGTCTTcc                                                              >  1:1361320/1‑151 (MQ=255)
                                                                                         cTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTcc                                                              >  3:179021/1‑151 (MQ=255)
                                                                                              tttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCCCCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAAAACTGTCTTACCcgcc                                                         >  8:1672496/1‑150 (MQ=255)
                                                                                               ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                         <  1:278004/150‑1 (MQ=255)
                                                                                                tctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCCCCAGCCGGCCATATTACACGGCGCTTTCAGCCACTGGCGGACAATACTGTCTTCCCCGCGa                                                        >  1:1296504/1‑150 (MQ=255)
                                                                                                 ggttcGCCGCGTCGGGGTTCCCCGCAACGTGCTGCATTTTATTGCCCG‑CCCCACCCGCCCCCCCCACCATATGCCCCACCACCACCCGCCACCCGGCCATAATCCACGGCGCTTTCAGCCAGTGGCGGACAATACCGGCTTCCCCGCGATa                                                      <  2:1366351/149‑1 (MQ=255)
                                                                                                  tttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCGCCCCCCCCATATTCACCACATACAGCCGCCAGCCGGCCATATTCCACCGCGCGATCCGCCAGGGGCGGACCATACTGTCGTGCCCGCCATa                                                      >  5:1590370/1‑150 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATCTTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                       <  7:337761/148‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                     <  2:233792/150‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATa                                                      <  5:996806/149‑1 (MQ=255)
                                                                                                   tgcgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                     <  1:592577/148‑1 (MQ=255)
                                                                                                     cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAt                                                   <  1:1097956/150‑1 (MQ=255)
                                                                                                     cgccgcGTCGGTGATCCCTGCAACGTGCGGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATCTGCACCACCTCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAtt                                                  <  6:915397/151‑1 (MQ=255)
                                                                                                        cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGCCCCACATCCAGCCGCCAGGCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGCTAAATttc                                                >  5:1529899/1‑150 (MQ=255)
                                                                                                        cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACCTCCAGCCGCCAGCCGTCCATATTCCCCGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttc                                                >  4:1013375/1‑150 (MQ=255)
                                                                                                            tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattc                                            <  3:859682/150‑1 (MQ=255)
                                                                                                             cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                           <  5:1522443/150‑1 (MQ=255)
                                                                                                               gTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCa                                        <  6:1077870/151‑1 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCG‑CGCCCCCCGCCGGCCCCCGCATATGGACCCCATCCACCCCCCCGCCGGGCCTATTGCACGGCGCGTTTAGCCAGTGGCGGGCAATAATGTGGTGCCCGCCGTAAAattaaataaacagc                                      >  8:306083/1‑140 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGa                                      <  8:1644745/151‑1 (MQ=255)
                                                                                                                  aTCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                     >  4:1194142/1‑151 (MQ=255)
                                                                                                                    cccGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACtt                                   <  3:1067307/151‑1 (MQ=255)
                                                                                                                     ccGGCAAGGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCGCCAGCATATGCACCACATCGAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGACATTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                  <  5:744077/151‑1 (MQ=255)
                                                                                                                        gCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                               <  6:1220943/151‑1 (MQ=255)
                                                                                                                        gCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACAT‑CAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                               >  3:59297/1‑150 (MQ=255)
                                                                                                                           aCGTGCTGCATATTCTTGCGCG‑CCCCACCCGCCTCCCCCTGCATATGGACCAGATCGAGCGGCCAGCCGTCCATATTGCACGGCGCTTTCAGCCAGTGTAGGAGAATACTGTGTTCCCCGCGATAAAATTCATTCACCAGACTTTCCGACt                            <  2:1149364/151‑1 (MQ=255)
                                                                                                                             gTGCTGCATATTATTGCGCG‑CCCCCCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGa                          <  5:32175/151‑1 (MQ=255)
                                                                                                                             gTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCAGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGa                          <  1:822949/151‑1 (MQ=255)
                                                                                                                               gctgcATATTATTGCGCG‑CCCCACCCGCCTCCCCCCGCATATGCACCACTTCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATa                        <  4:40970/151‑1 (MQ=255)
                                                                                                                               gctgcATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGAt                         <  1:343985/150‑1 (MQ=255)
                                                                                                                               gctgcATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCAAATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACCGCGCTTTCACCCAGTGGCGGACAATACTGTCTTCCCCGCCATAAATTTCATTCACCAGACTTTCCGACGGAt                         >  6:704123/1‑150 (MQ=255)
                                                                                                                                  gcATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAACTTTCATTCACCAGACTTTCCGACTGATaa                       <  6:963724/149‑1 (MQ=255)
                                                                                                                                  gcATATTATTGCGCG‑CCCCACCCCCCTCCCCCAGCATATGCACCACATACACCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCGTCCCCGCGATAAATTTAAATCACCAGACTTTACGACTGATAATc                     >  3:724671/1‑151 (MQ=255)
                                                                                                                                    atatTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCa                   <  6:787059/151‑1 (MQ=255)
                                                                                                                                    atatTATTGCGCG‑CCCCACCCGCCGCCCCCAGCAAATGCACCACATCCAGCCGCCAGCCGTGCATATTACACGGCGCTTTCAGCCCGTGGCGGACAAAACTGTCTTCCCCGCGATTAATTTAAATCACCAGACCTTCCGCCTGATAAtac                    >  8:558965/1‑148 (MQ=255)
                                                                                                                                      attattGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAg                  <  2:549093/150‑1 (MQ=255)
                                                                                                                                         attGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCtt               <  5:621919/150‑1 (MQ=255)
                                                                                                                                            gcgcg‑cCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCgg            <  5:1248212/150‑1 (MQ=255)
                                                                                                                                                gtccccACCGGCCTCCCCCTGCATATGCACCACATCCAGCCGCGTGCCGTGCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATTCTGTCTTGCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAg        <  6:1579143/149‑1 (MQ=255)
                                                                                                                                                g‑cCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGc       <  6:568010/151‑1 (MQ=255)
                                                                                                                                                   cccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg     >  7:1376850/1‑151 (MQ=255)
                                                                                                                                                    ccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGAGAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTgg    <  6:497783/151‑1 (MQ=255)
                                                                                                                                                     cACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   >  7:715737/1‑151 (MQ=255)
                                                                                                                                                     cACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   <  1:171008/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447254‑3447553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑