Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA23.3% 340.2 / 21.7 238intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (99/49);  new base A (42/3);  total (184/54)
Fisher's exact test for biased strand distribution p-value = 2.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121974‑4122272
                                                                                                                                                      |                                                                                                                                                    
gCTATACCAACTCTGCTATTCGCTTTGGGGTGGTGTGTTTCTTACATTTATTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGc                                                                                                                                                      <  8:1449866/151‑1 (MQ=14)
    aacaaaCTGAGCTTATCCCGAGTGGGGGGTGTGTCCCTTAAAGCACGTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTg                                                                                                                                                  <  7:1211814/147‑1 (MQ=17)
     acgacggcagcgcttCCCGCTTGCGGGGTCTGGGGCTCAGAGCTCTTGGAATCCTTGCTGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCttt                                                                                                                                                   <  1:15512/136‑1 (MQ=11)
       caacagcccTATTCCCGCGTGGGGGGTCTGTACCTTACAGGACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgcag                                                                                                                                               <  1:1504804/143‑1 (MQ=17)
       aaaCTCACCTATTCCCGCTTGGGTGGTGTGTTCCTTACATCACTATCACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgcag                                                                                                                                               <  6:201588/150‑1 (MQ=17)
              gCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACtttt                                                                                                                                        >  7:645960/1‑151 (MQ=255)
               cTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCCGTCGTATTTGTTGTGGAGCAATTTTc                                                                                                                                       >  5:972141/1‑151 (MQ=11)
                tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTTTGATGTGTAGCAATTTTc                                                                                                                                       >  8:1651106/1‑150 (MQ=17)
                 gttgccGCTTGTGTTTGCTTTGCCTTAGAGCTTTTTATATTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCa                                                                                                                                      <  4:763961/146‑1 (MQ=14)
                   tCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTGTGTGGTGCCGCACTTTTCAAAt                                                                                                                                   >  3:193673/1‑151 (MQ=17)
                   tCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTCGTCTGTTTTGTGGAGGACTTTTCCAAt                                                                                                                                   >  1:1223141/1‑151 (MQ=14)
                     ccgggtgtggggtCTGTCCCTTACAGCACTTGCAAATTTTGGCGCGGGAAACGAGAGTCGAACTCGCGACCCCGACCTTGGCTAGGTCGTTCTCTACCAACTGATCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAttt                                                                                                                                 <  7:903421/141‑1 (MQ=17)
                     ccgcgggggggctCTGTCCATTAAATCCCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAttt                                                                                                                                 <  5:1442508/139‑1 (MQ=14)
                     ccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGGGCGTGGTATGTGGGGTGAAGCAATTTTCAAAttt                                                                                                                                 >  6:1111464/1‑151 (MQ=14)
                       gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGATGTGGAGCAATTTTCGAAttttt                                                                                                                               >  1:976749/1‑150 (MQ=17)
                        cgtGGGCGGTCTGGCCCTTACAGTACTTTCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                              <  1:113390/149‑1 (MQ=17)
                        cTTGGGTGGTCAGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGACGTATCTGCTTTGCAGCACGTTTAAAATTATgg                                                                                                                              >  2:645271/1‑151 (MQ=17)
                         ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACGTCGTATTTTTTTTGAAGCAATATGCAAATTTTgg                                                                                                                              >  6:449287/1‑150 (MQ=11)
                             gtcctccgtgCCGTCCACCACTGTCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCgg                                                                                                                         <  1:291029/144‑1 (MQ=14)
                               ggtCTGTGCCTTACAGCAGTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGGACTTTTCAAATTTTGGAGTgggt                                                                                                                       >  5:577416/1‑150 (MQ=255)
                               ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTGTGGTTTGGAGCAATTTTCAAATTTTGGAgacgg                                                                                                                        >  1:77021/1‑146 (MQ=18)
                               gggcTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGa                                                                                                                       <  7:376222/148‑1 (MQ=255)
                                 tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTAAGTTATTTGCAGCAATTTTCAAATTTATGAGCGGGaa                                                                                                                      >  3:1436112/1‑150 (MQ=17)
                                 tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTATTTGGAGCACTTTTCCAAtttttgcgggggta                                                                                                                      >  7:881820/1‑140 (MQ=17)
                                   tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTCTGTGCAGCACTGGTCAAAATTTGCAGCGCGAAACg                                                                                                                   >  5:1667260/1‑151 (MQ=17)
                                     tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCTTATGTTGTGTGAAGCAATTTTCTCATTTTTGAggcggtaaggcc                                                                                                                 >  3:391512/1‑140 (MQ=14)
                                     tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGGAGGTCTGTGCGGTGCAGCAATTTTCAAATTTTGGCGCCGGAACCgcg                                                                                                                 >  1:452606/1‑149 (MQ=17)
                                     tgCCATACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCAGTTATATACAGAACTTTTCAAAtttatgagacgtaaagga                                                                                                                  >  6:659850/1‑135 (MQ=17)
                                        cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATGTTTTTTGAAGCACTTATCAAATTTTGGAGCGGGAAACGAGCCt                                                                                                              >  1:460522/1‑151 (MQ=17)
                                        cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATGTGATGTGCAGAGCTTTTAAAATTTTGGAGCGGGAAACGAGAc                                                                                                               >  5:1147259/1‑150 (MQ=17)
                                        cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACACGAACTCGCGACCCCGACCTTCGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCCGAGGTCTGTAATGTGAAGAAATTTTCAAATTTTTGAGACGGaacccggacc                                                                                                              >  4:164351/1‑143 (MQ=14)
                                         ttACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTATGTTGGGTGGAGGACTTTTCTAATTTTTGAGCGGGAACCgcgccgc                                                                                                             >  3:562340/1‑145 (MQ=14)
                                           aCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGCACTTTTCAAATTTTGGAGCCGGAAACGAGACTCg                                                                                                            >  3:559839/1‑150 (MQ=255)
                                            cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTTTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGAGTCGac                                                                                                          >  7:1491604/1‑150 (MQ=255)
                                            cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGATATGAAGAACTTTTCAAATTTTTGAGAGGGAACCGAGACTCGa                                                                                                           >  4:1284966/1‑150 (MQ=17)
                                                   ttCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTcgcg                                                                                                    >  7:1313306/1‑150 (MQ=255)
                                                   ttCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAATCCCGCTTGGGTGGTCTGTGGTTTGGAGCAATTTTCAAATTTTGGAGGGGGAAAGGCGCCTCGAACTcgcg                                                                                                    >  1:1623389/1‑150 (MQ=14)
                                                    tCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTGTGCTTTGGCGCACTTTTCTAATTTTGGAGCGGGAACCGAGAGTCGAACTCGCGAc                                                                                                  >  7:245178/1‑151 (MQ=14)
                                                      aaaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGTAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGGTTTGGAGGACTTTTCAAATTTTGGAGCGGGAACCGCGCCGGGATCTCGCGAccc                                                                                                >  7:741139/1‑151 (MQ=14)
                                                       aaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATGTTTGGTGGAGGACTTTTCCAATTTTGGAGCGGGAAACGCGCGGCGAACTCGCGAcccc                                                                                               >  5:1236260/1‑151 (MQ=14)
                                                       aaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGAGGTATGTGTTTTGCAGAAATTTTCAAATTTATGAGAGGGAAACGACACTCCAAATAACGAcca                                                                                                >  6:33402/1‑149 (MQ=14)
                                                         ttttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTGTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGCCTCGAACTCGCGACCACgc                                                                                             >  7:1399160/1‑150 (MQ=255)
                                                          tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTGTGTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCAGAc                                                                                            >  1:492695/1‑151 (MQ=14)
                                                          tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTGTGTTTTGCAGGACTTTTCAAATTTTTGAGGGGGTACCGCGGCTCGATCTCGCGACACCGAc                                                                                            >  5:360290/1‑151 (MQ=14)
                                                                    gAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGGTGGTCTGTGAGTTTGAGGACTTTTCGAATTTTGGAGCCGGAAACGGGGCTGGAACTCGCGACACAGCCCTAGGCCAgg                                                                                  >  6:1016386/1‑151 (MQ=11)
                                                                       aCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCg                                                                               >  5:234372/1‑151 (MQ=38)
                                                                        tgagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTCTTTGCAGCACTTTTCAAATTTTTGAGAGGGAAACGACACTCCAACTCGCGACCACGACATTGGAAAGGTcct                                                                              >  2:436384/2‑149 (MQ=14)
                                                                        cGAGACTGGAACGCGCGTCCCGGACGATGGCTCGGTCGTCCGCTACCAAATGAGGTATTCCCGGTTGGGTGGTCTGTGCTTTGCATCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGt                                                                              <  3:1013071/151‑1 (MQ=14)
                                                                         gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTg                                                                             >  7:1534153/1‑151 (MQ=255)
                                                                          agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACACGAACTCGCGACCCCGACCTTGGCAAGCTCCTGc                                                                            >  8:736511/1‑151 (MQ=255)
                                                                            tcTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGGACTTTTCTAATTTTTGAGCGGTAAACGAGACTGGAAATAGCGACAACGATCTTGGGAAGGTCGTGctg                                                                          >  2:796571/2‑150 (MQ=14)
                                                                            aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTATTTGAAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACACCTAAATTGGCAAGGTAGTGctc                                                                          >  6:365775/1‑151 (MQ=14)
                                                                                gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTAcc                                                                      >  4:264405/1‑151 (MQ=255)
                                                                                gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAcc                                                                      >  3:640636/1‑151 (MQ=38)
                                                                                  aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGACCTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCa                                                                     >  5:8325/1‑150 (MQ=255)
                                                                                  aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGAAGAAATTTTCAAATTTATGAGAGGGAAACGAGACTCGAACTCGCGACACAGAAATTGGAAAGGTCGTGCTCTACCa                                                                     >  2:1426823/1‑150 (MQ=14)
                                                                                   cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGATCTCGCGACACCGAGATTGGAAAGGTCGTGCTCTACAAAc                                                                   >  6:506263/1‑151 (MQ=14)
                                                                                     cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCGTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCGTGCGCTACCAACTg                                                                 >  3:78916/1‑151 (MQ=255)
                                                                                         cccccGCCCTTGCCAAGGTCGTGCTCTACCAACTCAGCTATTCCCGCTTGTTTTTTTTTTTATTTTAAGGAGTTTTCATATTTTTCATCTGGAAACGAGACTCTATCTCTAGACGACGATCTTGGGATGTTCGTGCTCTACCAACTGAGCt                                                             <  6:727079/150‑1 (MQ=2)
                                                                                         aCCCCGCCCTTGGCACGCTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATTTTCTTTTATTCACTTTTCAAATTTTGTAGCGGTAAACGATACTCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt                                                             <  8:93909/151‑1 (MQ=11)
                                                                                          ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTCGCGACCACGACCTTGGCAAGGTCGTGCGCTACCAACTGAGCt                                                             >  3:12009/1‑150 (MQ=255)
                                                                                             cGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGGTATTc                                                         >  5:1388430/1‑151 (MQ=255)
                                                                                              gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGCACTTTTCTAATTTTGGAGGGGGAACCGAGACTCGATCTCGCGACACCGACCTTGGCAAGGTCGACCGCTAACAACTTAGCTAttac                                                        >  3:125785/1‑149 (MQ=14)
                                                                                               aCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTGTTTGGAGCAATTTTCAAATTTATGAGAGGTAAACGAGCCTCGAACTAGAGAACCCGACATTGGCAAGGTCGTGATCTACCAACTGAGCTATTccc                                                       >  1:699199/1‑151 (MQ=14)
                                                                                                ccTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGGAAGGTCGGGCTCTACCAACTGAGCTATTCCcg                                                      >  2:1565876/1‑151 (MQ=255)
                                                                                                     gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCGAACTTCGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtac                                                 >  8:42766/1‑151 (MQ=255)
                                                                                                        aggtgttGCACTACCAACTGAGCTATGCCCGCTTGGGGGGTCTGGGCTTTGCAGCAGTTTTCAAATTTTGGAGGGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtactac                                              <  7:1222958/145‑1 (MQ=14)
                                                                                                        aGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATATTCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACACGAACACGCGACCCCGCACTTGGCAAGGTCGTCCACTACCAACTGATCTATTCGAGCTtactac                                              >  6:980474/1‑151 (MQ=11)
                                                                                                        aGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTGTTTGAAGCACTTTTCAAATTTTGGCGGGGGAAACGAGACTCGAACTAGCGACACCGCGCTTGGGAAGGTCCTCCTCTACCAACTGAGCTATTCCGGCGtaggac                                              >  2:1090390/1‑147 (MQ=14)
                                                                                                          gTCGTGCTCTCCCCACTGAGCTAGTCCCGCGTGGGGGGGGTGGGCCGTTCAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACGACtt                                            <  1:645984/151‑1 (MQ=11)
                                                                                                            cGTGCTCTACCAACTGAGCTATTCCCGCTTAGGTGTTATTTGATGTGAAGGACTTTTCAAATTTTTGAGAGGGAAACGAGACTCGAATTCGCGACCAAGAGATAGGAAAGCTACTCCTCTACAAACTGAGCTAATACCACGTACTACATa                                           >  7:1100338/1‑150 (MQ=9)
                                                                                                             gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCGCTACAAACTGAGCTATTCCAGCGTACTACTTAAt                                         >  5:1482493/1‑151 (MQ=255)
                                                                                                             gTGCTCTACCAACTGAGCTATTCCCGCTTCGGTCGTCTGTGCTATGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAAATCCCGACCCCGAACTTCGCAAGGACGTCCTCTACCAACTGAGATATTCCCGCGTAGTACTTAAt                                         >  1:600084/1‑151 (MQ=14)
                                                                                                              tGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTGTGCTTTGCCGCACTTTTCAAATTTTGGAGGGGGAACCGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGCTCGTCCTCTACCAACTGAGCTATTCCAGCGTactactatagt                                        >  2:1339925/1‑146 (MQ=14)
                                                                                                                     ccAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                 >  2:1670123/1‑151 (MQ=255)
                                                                                                                            aGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtt                          >  5:513197/1‑151 (MQ=255)
                                                                                                                             gCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAttt                         >  3:1561250/1‑151 (MQ=255)
                                                                                                                               tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtttt                        >  4:532212/1‑150 (MQ=255)
                                                                                                                               tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  3:676177/1‑151 (MQ=255)
                                                                                                                               tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  3:305287/1‑151 (MQ=255)
                                                                                                                               tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  5:820551/1‑151 (MQ=255)
                                                                                                                                 ttCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  4:676552/1‑151 (MQ=255)
                                                                                                                                 ttCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  4:301000/1‑151 (MQ=255)
                                                                                                                                  tccggtttGGGTGGTTTGTGATTTTGATTACTTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     <  6:479391/144‑1 (MQ=18)
                                                                                                                                       cTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt               >  1:40187/1‑151 (MQ=255)
                                                                                                                                        ttGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              >  4:1347804/1‑151 (MQ=255)
                                                                                                                                          gggTGCTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcg            >  6:1607111/1‑151 (MQ=255)
                                                                                                                                             tggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAGTTTCGCATTTCTGcgtcg         >  6:996945/1‑151 (MQ=255)
                                                                                                                                             tggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcg         >  2:125661/1‑151 (MQ=255)
                                                                                                                                              ggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgt           <  1:1034513/148‑1 (MQ=255)
                                                                                                                                              ggtCTGTGCTTTGCAGCACTTTTCAAATTTAGGAGCGGGAAACGACACTCGAACTCGCGACCCCGACCTTCGCAAGGTCGTGCTCTACCAACTCAGCTATTCCCGCGTACTACTTAAGTTTGCTTTCGTAATTTTCGCATTTCTGcgtcgt        >  8:1368217/1‑151 (MQ=255)
                                                                                                                                               gtCTGTGGTTTGCAGCACTTTACAATTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       <  8:1003956/151‑1 (MQ=255)
                                                                                                                                               gtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  8:1155482/1‑151 (MQ=255)
                                                                                                                                               gtCTGTGCTTTGCAGCACTTTTCAAATTTAGGAGCGGGAAACGACACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTCCTCTACCAACTGACCTAATCCCGCGTACTACTTAAATTTGCTTTCGTAATATTCGCAATTCTGCCACGtt       >  3:1015590/1‑151 (MQ=18)
                                                                                                                                                   gtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACgg   >  8:1645082/1‑151 (MQ=255)
                                                                                                                                                    tgCTTTGCAGCACTTTTCAGATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  8:31815/1‑151 (MQ=255)
                                                                                                                                                     gCTTTTCATCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  <  4:1113724/150‑1 (MQ=255)
                                                                                                                                                     gCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACCACACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCACTACCAACTGAGCTATTAACGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  4:421701/1‑150 (MQ=255)
                                                                                                                                                      cTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  8:396950/1‑149 (MQ=255)
                                                                                                                                                      cTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACcgg  >  4:1374368/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121974‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑