| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | DH1 | 236,086 | 0 | A | G | 76.9% | 19.0 / 3.8 | 13 | intergenic (+28/+26) | ECDH1_RS01120/gor | hypothetical protein/glutathione reductase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (6/4); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
GATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAAC > DH1/235941‑236220 | gATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAgcacac < 5:1183879/151‑1 (MQ=255) ggTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTc < 7:743152/151‑1 (MQ=255) aaGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACtcttc > 3:24599/1‑151 (MQ=255) gctgGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTc < 6:1414141/150‑1 (MQ=255) tgGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGa > 2:865676/1‑150 (MQ=255) gATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCtt < 1:1467130/149‑1 (MQ=255) aTCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCttttt < 3:1374656/151‑1 (MQ=255) aTCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCttttt > 8:949705/1‑151 (MQ=255) ccGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAACGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCAAAGTCTTTTTTg < 5:1151820/151‑1 (MQ=255) ttCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCAt > 3:40320/1‑151 (MQ=255) aGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCtt < 5:286764/151‑1 (MQ=255) tGATAGCGCAAACGTAATTAAGGGCTAAGAGCACACTACCCTTAGCCCTTTAACATTTAACGCAATGTAACGAACTCTTCTGCAGCCGAAGGGTGAATGGCGACGGTATTGTCGAAGTCATTTTTGGTTGCCCCCATCTTAAGCGCCAccc > 2:1102108/1‑150 (MQ=255) tAGCGGAAACGTAATTAAGGGCTAAGAGCACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACcgcg > 5:1128148/1‑150 (MQ=255) tAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAAc > 8:861765/1‑151 (MQ=255) | GATGCATTCTCACTCCATCGCATGGAGAAAACGGGTGATTGATAAAGCAATCATCGTTCTAGGGGCGTTAATTGCGCTGCTGGAACTGATCCGCTTTCTGCTTCAGCTTCTGAACTGATAGCGGAAACGTAATTAAGGGCTAAGAACACACTACTCTTAGCCCTTTAACATTTAACGCATTGTCACGAACTCTTCTGCCGCCGTTGGGTGAATGGCGACGGTATTGTCGAAGTCTTTTTTGGTTGCCCCCATCTTCAGCGCCACCGCGAAGCCCTGCAAC > DH1/235941‑236220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |