Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,479,764 G→T D79Y (GAT→TAT)  nuoH → NADH‑quinone oxidoreductase subunit H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,479,7640GT97.5% 1509.7 / 17.8 402D79Y (GAT→TAT) nuoHNADH‑quinone oxidoreductase subunit H
Reads supporting (aligned to +/- strand):  ref base G (8/2);  new base T (210/181);  total (219/183)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

GGCATTCATGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGGATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTGGCGGTTT  >  DH1/1479616‑1479911
                                                                                                                                                    |                                                                                                                                                   
ggCATTCATGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTAt                                                                                                                                                   <  7:752809/151‑1 (MQ=255)
     tCATGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATcgcg                                                                                                                                               <  1:880379/150‑1 (MQ=255)
      cATGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGt                                                                                                                                              <  3:234244/150‑1 (MQ=255)
      cATGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTc                                                                                                                                             >  5:1291547/1‑151 (MQ=255)
       aTGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTc                                                                                                                                             >  8:486999/1‑150 (MQ=255)
       aTGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTc                                                                                                                                             >  6:1112142/1‑150 (MQ=255)
       aTGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTc                                                                                                                                             >  3:2700/1‑150 (MQ=255)
        tGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCAt                                                                                                                                           >  8:189363/1‑151 (MQ=255)
         gAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATc                                                                                                                                          >  3:734615/1‑151 (MQ=255)
         gAGCTTTGGCGAACGGCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATc                                                                                                                                          <  3:1059662/151‑1 (MQ=255)
           gCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCtt                                                                                                                                        <  1:1109925/151‑1 (MQ=255)
               tGGCGAACGTCGCCTGGTGGGTCTGTTGCAGAAGCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTTGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCACGAAATTCTCGTATCGCGTCATCTTTAc                                                                                                                                     <  3:1356550/150‑1 (MQ=255)
               tGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTAc                                                                                                                                     <  1:381361/150‑1 (MQ=255)
                ggCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTAcc                                                                                                                                    <  4:1497491/150‑1 (MQ=255)
                 gCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCt                                                                                                                                  <  4:962215/151‑1 (MQ=255)
                    aaCGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGc                                                                                                                               <  5:1063816/151‑1 (MQ=255)
                    aaCGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGc                                                                                                                               >  7:787658/1‑151 (MQ=255)
                    aaCGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGGATCGCGTCATCTTTACCCTGGc                                                                                                                               >  4:1174361/1‑151 (MQ=255)
                      cGTCGCCTGCTGGGTCTGTTCCAGAACCGTTAAGGACCGACCCGTGTTGGCTGGGGAGGGTCGATCCAGCTGGTTGCGGACATGATCAAAATCTGCTTTAAAGACGACTGGATCCCGAAATTCTCCTATCGCGGCCTCATTACCCTGGCAc                                                                                                                             >  3:1404279/1‑151 (MQ=255)
                       gTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCAcc                                                                                                                            <  3:1167327/151‑1 (MQ=255)
                         cGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCga                                                                                                                          <  6:1588158/151‑1 (MQ=255)
                          gCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCgat                                                                                                                         >  8:686680/1‑151 (MQ=255)
                              gctgGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGAtt                                                                                                                     <  1:282165/151‑1 (MQ=255)
                              gctgGGGCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCgatgat                                                                                                                      <  4:400828/150‑1 (MQ=255)
                                 gggTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTg                                                                                                                    <  8:829922/149‑1 (MQ=255)
                                       gTTCCAGAACCGTTACGGACCTAAGCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTa                                                                                                              <  2:142752/149‑1 (MQ=255)
                                       gTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGAGTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTAcc                                                                                                            >  4:1324637/1‑151 (MQ=255)
                                       gTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTAcc                                                                                                            >  1:104969/1‑151 (MQ=255)
                                       gTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTAcc                                                                                                            >  6:484504/1‑151 (MQ=255)
                                       gTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTAACCTGTCAGCGATGATTGCCTTAAcc                                                                                                            >  7:823545/1‑151 (MQ=255)
                                         cccAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTc                                                                                                          <  2:876416/150‑1 (MQ=255)
                                          ccAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTc                                                                                                          <  1:164410/150‑1 (MQ=255)
                                            aGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGGATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCgct                                                                                                       >  6:1175395/1‑151 (MQ=255)
                                               aCCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCgctgct                                                                                                    <  1:1224205/151‑1 (MQ=255)
                                                    tACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGctgctgctg                                                                                                <  5:1354409/150‑1 (MQ=255)
                                                       ggACCTAACCGTGTTGGCTGGGGCGGGTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCGGCTGGCCt                                                                                            <  2:846660/151‑1 (MQ=255)
                                                         aCCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCttt                                                                                          >  5:246458/1‑151 (MQ=255)
                                                          ccTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTg                                                                                         <  7:65180/151‑1 (MQ=255)
                                                              aCCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATNCCGAAATNCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGAt                                                                                     >  6:1390388/1‑151 (MQ=255)
                                                              aCCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGAt                                                                                     <  8:1219388/151‑1 (MQ=255)
                                                                cGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTGTGCGATtg                                                                                   >  7:237462/1‑151 (MQ=255)
                                                                  tgtTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATtgtg                                                                                 >  5:1655671/1‑151 (MQ=255)
                                                                   gtTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATtgtg                                                                                 <  3:780442/150‑1 (MQ=255)
                                                                       gCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGGATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCcagt                                                                            >  1:531026/1‑151 (MQ=255)
                                                                          ggggCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCcagtcag                                                                         <  3:63320/151‑1 (MQ=255)
                                                                          ggggCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCcagtc                                                                           >  2:1546821/1‑149 (MQ=255)
                                                                            ggCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCagtcagtc                                                                       <  3:367017/151‑1 (MQ=255)
                                                                              cGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCg                                                                     <  7:1411004/151‑1 (MQ=255)
                                                                              cGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGGATCGCGGCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCg                                                                     <  8:23044/151‑1 (MQ=255)
                                                                                  tCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACACTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCATTTGCGATTGTGCCAGTCAGTCCGGGtt                                                                 >  4:576978/1‑151 (MQ=255)
                                                                                     cTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTggg                                                              >  5:95770/1‑151 (MQ=255)
                                                                                      cccAGCTGGTTGCGCACATGACCAAAATGTTCTTTAAAGAAGACTCGATCCCGAAATTCTCGTCTCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGGCCGGGTTGggt                                                             <  8:365337/150‑1 (MQ=255)
                                                                                       ccAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGggtg                                                            >  2:510529/1‑151 (MQ=255)
                                                                                        cAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGggtgg                                                           <  2:14896/151‑1 (MQ=255)
                                                                                          gCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGtt                                                         <  2:1226533/151‑1 (MQ=255)
                                                                                           cTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGCGTTGGGTGGTTg                                                        >  4:523103/1‑151 (MQ=255)
                                                                                            tGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGc                                                       >  1:970488/1‑151 (MQ=255)
                                                                                             ggTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGcc                                                      >  7:1647399/1‑151 (MQ=255)
                                                                                              gTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCg                                                     >  7:1157010/1‑151 (MQ=255)
                                                                                                tGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGAc                                                   >  5:756899/1‑151 (MQ=255)
                                                                                                     aCATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGa                                               >  4:814123/1‑150 (MQ=255)
                                                                                                       aTGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGCCCTGAAc                                             >  2:440596/1‑150 (MQ=255)
                                                                                                       aTGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAAc                                             >  3:394854/1‑150 (MQ=255)
                                                                                                       aTGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACa                                            <  7:41128/151‑1 (MQ=255)
                                                                                                         gATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACAt                                           <  8:1033965/150‑1 (MQ=255)
                                                                                                            cAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGCCCTGAACATCggg                                       >  2:706951/1‑151 (MQ=255)
                                                                                                            cAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCggg                                       >  6:685125/1‑151 (MQ=255)
                                                                                                             aaaaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCggg                                       >  1:228555/1‑150 (MQ=255)
                                                                                                             aaaaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCggg                                       >  6:1501581/1‑150 (MQ=255)
                                                                                                             aaaaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGAGGTTGCCGCCCTGAACATCggg                                       >  6:797198/1‑150 (MQ=255)
                                                                                                              aaaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAt                                     >  6:1310498/1‑151 (MQ=255)
                                                                                                              aaaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAt                                     <  7:551824/151‑1 (MQ=255)
                                                                                                              aaaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAt                                     >  4:1431198/1‑151 (MQ=255)
                                                                                                               aaTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAtt                                    <  7:1085057/151‑1 (MQ=255)
                                                                                                                aTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAttt                                   <  1:1564140/151‑1 (MQ=255)
                                                                                                                aTGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAttt                                   <  1:1381832/151‑1 (MQ=255)
                                                                                                                 tGTTCTTTATAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCCGGTTGGGTGGTTGGGGACCTGAACATCGGGAttt                                   <  5:338907/150‑1 (MQ=255)
                                                                                                                  gTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCTTCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGAtttt                                  <  2:185880/150‑1 (MQ=255)
                                                                                                                    tCTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGtt                               >  4:76190/1‑151 (MQ=255)
                                                                                                                     cTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGttc                              >  8:117674/1‑151 (MQ=255)
                                                                                                                     cTTTAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCATGGCACCGATGTTTCCCTATACGTCGCTGCTGCTGGCGTTTGCGCTTGTCCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGCACTTCGGGGTTTGGttg                              >  5:1439217/1‑150 (MQ=255)
                                                                                                                       ttAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGttctt                            >  2:1146271/1‑151 (MQ=255)
                                                                                                                       ttAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGtt                               <  1:783100/148‑1 (MQ=255)
                                                                                                                       ttAAAGAAGACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGtt                               <  5:574258/148‑1 (MQ=255)
                                                                                                                          aagaagACTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCt                         >  4:1640835/1‑151 (MQ=255)
                                                                                                                           agaagaCTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCtg                        <  5:64193/151‑1 (MQ=255)
                                                                                                                             aagaCTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCtgat                      >  6:278369/1‑151 (MQ=255)
                                                                                                                             aagaCTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCtgat                      >  4:286240/1‑151 (MQ=255)
                                                                                                                                aCTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTgatgat                   >  6:352990/1‑151 (MQ=255)
                                                                                                                                aCTGGATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTgatgat                   <  4:864462/151‑1 (MQ=255)
                                                                                                                                   ggATCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGatgatg                  <  8:590387/149‑1 (MQ=255)
                                                                                                                                     aTCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGc                >  3:1500573/1‑149 (MQ=255)
                                                                                                                                     aTCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGc                >  8:1084121/1‑149 (MQ=255)
                                                                                                                                     aTCCCGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAg              <  7:275576/151‑1 (MQ=255)
                                                                                                                                         cGAAATTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGGGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCt          <  8:1592258/151‑1 (MQ=255)
                                                                                                                                           aaaTTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTgg        >  5:458138/1‑151 (MQ=255)
                                                                                                                                            aaTTCTCGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGGTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTGGc       <  3:888861/151‑1 (MQ=255)
                                                                                                                                               tctcGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTGGCgg     <  8:1471593/150‑1 (MQ=255)
                                                                                                                                               tctcGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTGGCgg     <  6:54235/150‑1 (MQ=255)
                                                                                                                                               tctcGTATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTGGCgg     <  3:837198/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCATTCATGAGCTTTGGCGAACGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCGTGTTGGCTGGGGCGGTTCGCTCCAGCTGGTTGCGGACATGATCAAAATGTTCTTTAAAGAAGACTGGATCCCGAAATTCTCGGATCGCGTCATCTTTACCCTGGCACCGATGATTGCCTTTACCTCGCTGCTGCTGGCCTTTGCGATTGTGCCAGTCAGTCCGGGTTGGGTGGTTGCCGACCTGAACATCGGGATTTTGTTCTTCCTGATGATGGCAGGTCTGGCGGTTT  >  DH1/1479616‑1479911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑