Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,993,533 A→G L55S (TTG→TCG)  rcdA ← DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,993,5330AG97.6% 1570.8 / 13.5 420L55S (TTG→TCG) rcdADNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (5/5);  new base G (214/195);  total (220/200)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

TCCATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCGGAT  >  DH1/2993386‑2993680
                                                                                                                                                   |                                                                                                                                                   
tCCATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGACTGGCATGCGCCCTGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGATCGCCTCAAGTAACGACt                                                                                                                                                  >  7:847106/1‑151 (MQ=255)
  cATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtca                                                                                                                                                <  1:910305/151‑1 (MQ=255)
  cATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtc                                                                                                                                                 >  6:1118599/1‑150 (MQ=255)
   aTGTTATCCGGCGTTGCAACCTGTGAGCTGTATATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtcat                                                                                                                                               <  7:43558/151‑1 (MQ=255)
   aTGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACtcat                                                                                                                                               >  5:741786/1‑151 (MQ=255)
    tGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtcatc                                                                                                                                              >  1:76070/1‑151 (MQ=255)
       tATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATTGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtcatcat                                                                                                                                            <  7:263204/150‑2 (MQ=255)
       tATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCaa                                                                                                                                            <  6:62379/150‑1 (MQ=255)
        aTCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGACCGCCTCCAGTAACGACTCATCAAtt                                                                                                                                          >  8:1240615/1‑151 (MQ=255)
        aTCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCT‑ATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAAtt                                                                                                                                          <  1:1319938/150‑1 (MQ=255)
           cGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCt                                                                                                                                       <  6:305373/151‑1 (MQ=255)
            ggCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTg                                                                                                                                      >  6:445537/1‑151 (MQ=255)
             gCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGa                                                                                                                                     <  2:923479/151‑1 (MQ=255)
               gTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGaaa                                                                                                                                   <  5:1380375/151‑1 (MQ=255)
               gTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCCAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGaa                                                                                                                                    >  3:544060/1‑150 (MQ=255)
                   cAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAta                                                                                                                               <  1:1380070/151‑1 (MQ=255)
                    aaCCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAt                                                                                                                                >  2:1319502/1‑149 (MQ=255)
                      ccTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGATCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGCGACATGATCTCAGTAAAACTGCTGAACGCCTCCCGTAACGACTCATCAATTCCTGAAAAAtagta                                                                                                                            <  1:442315/151‑1 (MQ=255)
                      ccTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAtagta                                                                                                                            <  4:362298/151‑1 (MQ=255)
                      ccTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAtagta                                                                                                                            <  2:476341/151‑1 (MQ=255)
                         gtgAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGt                                                                                                                         <  6:1648419/151‑1 (MQ=255)
                          tgAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTc                                                                                                                        >  7:1009377/1‑151 (MQ=255)
                           gAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTc                                                                                                                        <  8:1435515/150‑1 (MQ=255)
                            aGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCAt                                                                                                                      >  2:293114/1‑151 (MQ=255)
                            aGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCAt                                                                                                                      <  8:656579/151‑1 (MQ=255)
                               tGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCg                                                                                                                    >  7:1647040/1‑150 (MQ=255)
                               tGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCg                                                                                                                    >  5:1502037/1‑150 (MQ=255)
                                gTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCg                                                                                                                    <  5:1644001/149‑1 (MQ=255)
                                gTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGa                                                                                                                   <  6:69033/150‑1 (MQ=255)
                                gTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGAt                                                                                                                  <  6:548020/151‑1 (MQ=255)
                                  aGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCTTCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCATTAAAACTGCTTAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATc                                                                                                                 <  7:667614/150‑1 (MQ=255)
                                  aGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATcc                                                                                                                >  5:1221757/1‑151 (MQ=255)
                                  aGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATcc                                                                                                                <  1:1174864/151‑1 (MQ=255)
                                      cATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCaa                                                                                                             <  2:602233/150‑1 (MQ=255)
                                       atatTGGTGATTGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACg                                                                                                           >  6:1399930/1‑151 (MQ=255)
                                         atgggTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACgg                                                                                                          <  2:790347/147‑1 (MQ=255)
                                         atCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGt                                                                                                         <  5:572124/151‑1 (MQ=255)
                                          tCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGt                                                                                                         >  4:1336055/1‑150 (MQ=255)
                                             gTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTAccc                                                                                                     >  6:1288543/1‑151 (MQ=255)
                                                     cTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAgg                                                                                             >  4:751818/1‑151 (MQ=255)
                                                       ggCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAggg                                                                                            <  5:446131/150‑1 (MQ=255)
                                                       ggCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAgg                                                                                             <  2:1452674/149‑1 (MQ=255)
                                                       ggCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGt                                                                                           >  3:2949/1‑151 (MQ=255)
                                                        gCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTa                                                                                          <  1:1025368/151‑1 (MQ=255)
                                                         cATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGt                                                                                           >  3:1632290/1‑149 (MQ=255)
                                                                 cGGATCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAACCTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTg                                                                                 <  7:542539/151‑1 (MQ=255)
                                                                 cGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTg                                                                                 >  3:831821/1‑151 (MQ=255)
                                                                  ggAGCATCACTTACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTTAACGCCTCCAGTAACGACTCCTCAATTCCTGAAAAATAGTAGGTCATCGAACCCAACGGTACCCCGCCAAGCGTAGCATTATTGc                                                                                >  4:1078883/1‑151 (MQ=255)
                                                                  ggAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGc                                                                                >  6:218085/1‑151 (MQ=255)
                                                                   gAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCg                                                                               <  5:352980/151‑1 (MQ=255)
                                                                    aGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCgg                                                                              <  4:876816/151‑1 (MQ=255)
                                                                    aGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCgg                                                                              >  7:1464810/1‑151 (MQ=255)
                                                                      cATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTg                                                                            <  7:1156114/151‑1 (MQ=255)
                                                                        tCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCg                                                                          >  3:1152665/1‑151 (MQ=255)
                                                                         cACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAATGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCg                                                                          <  5:864224/150‑1 (MQ=255)
                                                                         cACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCACAACCGTACCCCGGCAAGGGTAGCAATTTTGAGGTGCGt                                                                         >  8:798179/1‑151 (MQ=255)
                                                                             aaCATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAAc                                                                      >  1:260168/1‑150 (MQ=255)
                                                                              aCATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTCCCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAAc                                                                      >  8:1292254/1‑149 (MQ=255)
                                                                              aCATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAg                                                                    <  3:1452896/151‑1 (MQ=255)
                                                                                aTCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCa                                                                  <  3:492773/151‑1 (MQ=255)
                                                                                   gCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGt                                                               >  5:1044531/1‑151 (MQ=255)
                                                                                   gCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGt                                                               <  4:168519/151‑1 (MQ=255)
                                                                                     tAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTAt                                                             >  3:1593000/1‑151 (MQ=255)
                                                                                      aaaaaaTGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTAtt                                                            >  3:217652/1‑151 (MQ=255)
                                                                                           aTGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATTGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGt                                                        >  2:1085586/1‑150 (MQ=255)
                                                                                           aTGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTa                                                       <  3:1101832/151‑1 (MQ=255)
                                                                                            tGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTa                                                       >  3:996654/1‑150 (MQ=255)
                                                                                             gCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTaaa                                                     <  7:105874/151‑1 (MQ=255)
                                                                                             gCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTaa                                                      >  1:1100525/1‑150 (MQ=255)
                                                                                                tGATATTGCCGGGACATGATCTCAGTAAAACTGCAGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAAAGGTACACCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGtt                                                  >  5:787914/1‑151 (MQ=255)
                                                                                                 gATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGGCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGt                                                   <  1:666896/149‑1 (MQ=255)
                                                                                                     ttGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCACAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCAcc                                             >  2:1385670/1‑151 (MQ=255)
                                                                                                      tGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCg                                            >  1:1667120/1‑151 (MQ=255)
                                                                                                       gCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCg                                            <  1:1590853/150‑1 (MQ=255)
                                                                                                       gCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGc                                           >  8:158648/1‑151 (MQ=255)
                                                                                                           ggACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTcc                                       >  7:397288/1‑151 (MQ=255)
                                                                                                           ggACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTcc                                       <  3:697431/151‑1 (MQ=255)
                                                                                                            gACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTcc                                       >  3:313734/1‑150 (MQ=255)
                                                                                                              cATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTACGTAAAGTTTCACCGCCTCCAGc                                    >  1:1517524/1‑151 (MQ=255)
                                                                                                               aTGATCTCAGTAAAACTGCTGAACGCCTCCAGTACCGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGGGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCg                                   >  5:201026/1‑151 (MQ=255)
                                                                                                                tGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGt                                  >  7:1323868/1‑151 (MQ=255)
                                                                                                                 gATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                 >  8:1477988/1‑151 (MQ=255)
                                                                                                                  aTCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTgg                                >  7:753180/1‑151 (MQ=255)
                                                                                                                      cAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                 <  2:1626713/146‑1 (MQ=255)
                                                                                                                      cAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                 <  4:1516979/146‑1 (MQ=255)
                                                                                                                      cAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCt                             <  6:1506674/150‑1 (MQ=255)
                                                                                                                      cAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                 <  6:740885/146‑1 (MQ=255)
                                                                                                                       aGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTgg                           <  6:471877/151‑1 (MQ=255)
                                                                                                                        gTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGa                          <  8:1142221/151‑1 (MQ=255)
                                                                                                                           aaaCTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATa                        >  4:241068/1‑150 (MQ=255)
                                                                                                                             aCTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtt                     >  6:855823/1‑151 (MQ=255)
                                                                                                                             aCTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtt                     <  2:394345/151‑1 (MQ=255)
                                                                                                                                gctgAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAttttt                  >  8:465270/1‑151 (MQ=255)
                                                                                                                                gctgAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtttt                   <  8:1644984/150‑1 (MQ=255)
                                                                                                                                gctgAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAttt                    <  1:992464/149‑1 (MQ=255)
                                                                                                                                   gAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtttttt                 <  4:689815/149‑1 (MQ=255)
                                                                                                                                       gCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGcc           >  6:1569835/1‑151 (MQ=255)
                                                                                                                                          cccAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGtt        <  8:307037/150‑1 (MQ=255)
                                                                                                                                             aGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGGAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCg     >  3:1260865/1‑151 (MQ=255)
                                                                                                                                             aGTAACGACTCATCAATTCCTGAAAAATAGTAGGGCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCg     <  6:1446063/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCGGAT  >  DH1/2993386‑2993680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑