Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,232,214 Δ1 bp intergenic (‑141/‑392) flxA ← / → relB protein FlxA/antitoxin RelB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,232,2110G.99.5% 1977.6 / 0.9 424intergenic (‑138/‑395)flxA/relBprotein FlxA/antitoxin RelB
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (225/197);  total (226/198)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 444 total aligned reads displayed.

TGTGACAGACATAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAATAGATGAATAAGT  >  DH1/2232062‑2232356
                                                                                                                                                     |                                                                                                                                                 
tgtgACAGACATAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAgg                                                                                                                                                  >  6:899201/1‑151 (MQ=255)
     cagacaTAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAgggtaa                                                                                                                                              >  5:1557730/1‑147 (MQ=255)
       gacaTAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACg                                                                                                                                          <  6:793836/151‑1 (MQ=255)
       gacaTAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACg                                                                                                                                          <  8:1003337/151‑1 (MQ=255)
        acaTAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTACAGATTGTTTTCCTCGCGGGCTGGAGCGCCTCAGAAAGTAAAGCTTGTGGACAGGGTAAACGt                                                                                                                                         >  5:841946/1‑151 (MQ=255)
         caTAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGtt                                                                                                                                        >  3:860685/1‑151 (MQ=255)
            aGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCg                                                                                                                                      <  3:1572202/150‑1 (MQ=255)
             gATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGc                                                                                                                                    <  5:1276471/151‑1 (MQ=255)
              aTTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCATCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGc                                                                                                                                    >  2:596162/1‑150 (MQ=255)
                 tctcCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCaat                                                                                                                                 <  1:1070913/150‑1 (MQ=255)
                   tcCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCaataat                                                                                                                              >  1:1512941/1‑151 (MQ=255)
                     cTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAAttt                                                                                                                            >  3:279416/1‑151 (MQ=255)
                        ttAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTc                                                                                                                          <  4:692401/150‑1 (MQ=255)
                        ttAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCt                                                                                                                         >  1:747313/1‑151 (MQ=255)
                          aaaaaaGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTg                                                                                                                        <  8:674409/150‑1 (MQ=255)
                             aaaGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGc                                                                                                                    >  5:176957/1‑151 (MQ=255)
                                  aCTATCGGCATGCACAAAAAAATCTTTCATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCg                                                                                                               <  1:1283425/151‑1 (MQ=255)
                                  aCTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCg                                                                                                               >  3:1395416/1‑151 (MQ=255)
                                    tATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCggg                                                                                                             >  3:972509/1‑151 (MQ=255)
                                     aTCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGgt                                                                                                            >  6:1009519/1‑151 (MQ=255)
                                       cGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGgtgt                                                                                                          >  6:33/1‑151 (MQ=255)
                                        ggCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGtt                                                                                                         <  6:1415758/151‑1 (MQ=255)
                                          cATGCACAAAAAAATCTTTAATCTTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGTCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGc                                                                                                       <  6:1211581/151‑1 (MQ=255)
                                          cATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGc                                                                                                       >  6:1820402/1‑151 (MQ=255)
                                           aTGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGc                                                                                                       >  1:465768/1‑150 (MQ=255)
                                             gCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAAGGTTCGGGAATAATTTTCTGCCGCATGCGGGTGTTGCAt                                                                                                     <  7:733331/150‑1 (MQ=255)
                                             gCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCAt                                                                                                     >  7:555331/1‑150 (MQ=255)
                                              cacaAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATaa                                                                                                   <  7:843408/151‑1 (MQ=255)
                                              cacaAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATaa                                                                                                   <  8:397479/151‑1 (MQ=255)
                                              cacaAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATa                                                                                                    >  4:1683353/1‑150 (MQ=255)
                                               acaAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATaa                                                                                                   >  8:586646/1‑150 (MQ=255)
                                                caAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATaaa                                                                                                  >  2:888409/1‑150 (MQ=255)
                                                caAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATaaa                                                                                                  >  8:1231196/1‑150 (MQ=255)
                                                 aaaaaaaTCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAAc                                                                                                <  3:484731/151‑1 (MQ=255)
                                                  aaaaaaTCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACg                                                                                               >  1:55786/1‑151 (MQ=255)
                                                  aaaaaaTCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACg                                                                                               >  5:531229/1‑151 (MQ=255)
                                                     aaaTCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACgtgt                                                                                            >  7:1591828/1‑151 (MQ=255)
                                                      aaTCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGtt                                                                                           >  7:1324182/1‑151 (MQ=255)
                                                      aaTCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGtt                                                                                           >  5:1115510/1‑151 (MQ=255)
                                                        tCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTAc                                                                                         >  7:927284/1‑151 (MQ=255)
                                                        tCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTAc                                                                                         >  3:1510690/1‑151 (MQ=255)
                                                           ttAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTAc                                                                                         <  2:1568928/148‑1 (MQ=255)
                                                           ttAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGtt                                                                                      >  7:1090740/1‑151 (MQ=255)
                                                           ttAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGt                                                                                       <  2:579612/150‑1 (MQ=255)
                                                            tAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGtt                                                                                      <  4:84930/150‑1 (MQ=255)
                                                            tAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTc                                                                                     >  8:1415717/1‑151 (MQ=255)
                                                             aaTCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTcc                                                                                    >  4:138369/1‑151 (MQ=255)
                                                              aTCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCt                                                                                   <  1:1185442/151‑1 (MQ=255)
                                                                  tATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTAt                                                                               >  3:1063839/1‑151 (MQ=255)
                                                                   aTTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATc                                                                              <  8:1500931/151‑1 (MQ=255)
                                                                     ttCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCg                                                                             >  2:1103257/1‑150 (MQ=255)
                                                                       cTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGAc                                                                           >  3:1443998/1‑150 (MQ=255)
                                                                         tgtgTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGAcagg                                                                        >  6:1264854/1‑151 (MQ=255)
                                                                         tgtgTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGAca                                                                          <  6:1553233/149‑1 (MQ=255)
                                                                          gtgtCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGAcagg                                                                        <  3:369281/150‑1 (MQ=255)
                                                                             tCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGAcaggtc                                                                      >  5:1165491/1‑149 (MQ=255)
                                                                              cATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGAcaggtcagg                                                                   >  1:1718217/1‑151 (MQ=255)
                                                                               aTTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGACGCATGCGGGTGTTGCATAAAAAGTGTTACGTTCCTTTATCGAcaggtcaggt                                                                  >  3:733636/1‑151 (MQ=255)
                                                                                  aaTTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGAGAGGGTAAACGTTCGGCAATAATTTTCTGGCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCAc                                                               <  2:1236488/151‑1 (MQ=255)
                                                                                        ttGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTcc                                                         >  8:1332049/1‑150 (MQ=255)
                                                                                          gATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCAcc                                                       >  1:321047/1‑151 (MQ=255)
                                                                                           aggTTCAGATGGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCAccc                                                      <  6:603616/149‑1 (MQ=255)
                                                                                           aTGTTCAGATTATTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTACACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCAccc                                                      >  7:487956/1‑151 (MQ=255)
                                                                                             gTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTAACCGCTCACccgc                                                    >  2:1708086/1‑151 (MQ=255)
                                                                                              ttCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACATTCGGCAATAATTTTCTGGCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACccgcc                                                   >  2:1381445/1‑151 (MQ=255)
                                                                                               tCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACccgccg                                                  >  3:578331/1‑151 (MQ=255)
                                                                                                 aGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCcgac                                                >  4:1133393/1‑151 (MQ=255)
                                                                                                 aGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCcgac                                                >  1:1585137/1‑151 (MQ=255)
                                                                                                      gTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGaa                                           <  5:1823445/151‑1 (MQ=255)
                                                                                                      gTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGGCACCGCTCACCCGCCGACgaga                                            <  4:1562817/150‑1 (MQ=255)
                                                                                                       ttttCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGaa                                           >  2:1437065/1‑150 (MQ=255)
                                                                                                        tttCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGaaa                                          <  8:168868/150‑1 (MQ=255)
                                                                                                        tttCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCACTCACCCGCCGACGAGaa                                           >  5:1598561/1‑149 (MQ=255)
                                                                                                           ccTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCGATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCa                                       <  3:1702631/150‑1 (MQ=255)
                                                                                                           ccTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCaa                                      <  8:1081392/151‑1 (MQ=255)
                                                                                                            cTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGAAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACACGCCGACGAGAAAGCAac                                     >  2:1609988/1‑151 (MQ=255)
                                                                                                               gcgGGCTGGCGCGCGTCAGAAAGTAAAGCTTGTTGACAGGGTATAGGTTCGGCAATAATTTTCTGCGGCATGCGGGGGTTGCATATAACGTGTTACGTTGCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACt                                  <  4:1787612/151‑1 (MQ=255)
                                                                                                                cgGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTg                                 <  3:1220626/151‑1 (MQ=255)
                                                                                                                  ggCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGAc                               <  5:338328/151‑1 (MQ=255)
                                                                                                                   gCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGCTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGGTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACa                              <  4:616802/151‑1 (MQ=255)
                                                                                                                      ggCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATg                            <  7:320761/150‑1 (MQ=255)
                                                                                                                      ggCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGc                           <  8:198798/151‑1 (MQ=255)
                                                                                                                        cgcgcCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCt                          >  5:1433027/1‑150 (MQ=255)
                                                                                                                        cgcgcCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTa                         <  7:1220616/151‑1 (MQ=255)
                                                                                                                          cgcCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTaa                        >  6:393021/1‑150 (MQ=255)
                                                                                                                          cgcCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGATaa                        >  4:364967/1‑150 (MQ=255)
                                                                                                                           gcCTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAg                      <  4:1079824/151‑1 (MQ=255)
                                                                                                                            ccTCAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGc                     <  7:196755/151‑1 (MQ=255)
                                                                                                                               cAGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCaa                   >  4:470772/1‑150 (MQ=255)
                                                                                                                                ataaaGTAAAGCTTGTTGACAGGCTAAAAGTTCCGAAATAATATTCTGCCGCATGCTCGTGTTGCATAAAACGTCTTACTAAACTATATCAACAGGTCAGCTCACCGATCACACGCCCACGAGAAACCAACACTGACATGCTAAAGCaaa                  >  2:382205/3‑150 (MQ=255)
                                                                                                                                aGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCaaa                  >  4:1485523/1‑150 (MQ=255)
                                                                                                                                aGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCaaa                  >  5:1222905/1‑150 (MQ=255)
                                                                                                                                aGAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGGGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCaaaa                 <  4:973337/151‑1 (MQ=255)
                                                                                                                                 gAAAGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCaaaaa                <  6:199868/151‑1 (MQ=255)
                                                                                                                                  aaaGTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCaaaaa                >  3:198133/1‑150 (MQ=255)
                                                                                                                                     gTAAAGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAAt              >  5:583382/1‑149 (MQ=255)
                                                                                                                                         aGCTTGTTGACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAATAGATGa        <  6:513941/151‑1 (MQ=255)
                                                                                                                                             tgttgACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAATAGATGAAt      <  2:748090/149‑1 (MQ=255)
                                                                                                                                              gttgACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAATAGATGAATaa    <  8:842840/150‑1 (MQ=255)
                                                                                                                                              gttgACAGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAATAGATGAATa     >  6:1624095/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TGTGACAGACATAGATTTCTCCTTTTAAAAAAGCACTATCGGCATGCACAAAAAAATCTTTAATCGTATTTCTTGTGTCATTAATTGTTTGATGTTCAGATTGTTTTCCTCGCGGGCTGGCGCGCCTCAGAAAGTAAAGCTTGTTGACAGGGGTAAACGTTCGGCAATAATTTTCTGCCGCATGCGGGTGTTGCATAAAACGTGTTACGTTCCTTTATCGACAGGTCAGGTCACCGCTCACCCGCCGACGAGAAAGCAACACTGACATGCTAAAGCAAAAAATAGATGAATAAGT  >  DH1/2232062‑2232356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: