Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8320CA33.0% 148.7 / 16.0 97intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (49/12);  new base A (28/2);  total (83/14)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTT  >  DH1/3906693‑3906982
                                                                                                                                           |                                                                                                                                                        
tCAAGGCCCCCGCCTCGCACCAAAAACCTCGTTGTTATTCCTGTCTCTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                                <  8:1332402/150‑1 (MQ=18)
gcAAGTCCCCCCCCTCGCACCAAAAACCACGTTTATATTCCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATc                                                                                                                                               <  8:406408/150‑1 (MQ=255)
gcAAGGCCCCCCCCTCGCACGAAAAACCACGTTTATATTGCTCGCACTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                                <  7:539310/149‑1 (MQ=255)
          ccccTCGCACCAAAAAACAGGTTGATATTGCTAGAAATGGGCGAAGGTTTAGGAAAAGGTATACACAATAGCTTCAGGTGTTAGTGTTATTACGGACGTGGGGGTTCAAGTACCACCCCTCGCGCCAAAAAAAGATAAATAAAAAAAAGt                                                                                                                                      >  6:892699/1‑150 (MQ=255)
             cTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGAAGGGTACATCCAAAAAAGTAAgc                                                                                                                                  >  3:1575106/1‑150 (MQ=18)
              tCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATAGGGTATATCaattaatgtttgat                                                                                                                                 >  5:634817/1‑139 (MQ=18)
                gCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACTCGGCCATCCCAaagaaagaaagaggc                                                                                                                               >  5:861562/1‑148 (MQ=18)
                gCACAAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGGTAAATCaaaaaaatttacatt                                                                                                                                >  4:962744/1‑142 (MQ=17)
                 gtgctatcacgtcGTTGTTATTGCTCTCTGTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGAc                                                                                                                              <  8:1322523/139‑1 (MQ=17)
                   ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGTTTAAATCAATAAAAGtttcatcaatt                                                                                                                            >  2:234257/1‑141 (MQ=17)
                     aaaaaCCACGGTGATTTTGCTGCCTGTGGGCTTTGCTGGCGGTTTTGGTAGCCGGGTTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                          <  5:1818207/151‑1 (MQ=18)
                      aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATAGGGTAGATCAAAAAATGTTTGATgcgtgttg                                                                                                                         >  3:208260/1‑144 (MQ=17)
                      aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGCGACATCCAAAAATGttacatccatgttt                                                                                                                         >  5:135259/1‑138 (MQ=17)
                       aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAACACGCGATATCAAATAAAGTTTGATGCAtgtggt                                                                                                                        >  5:790209/1‑149 (MQ=18)
                          ccACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACTTGGAGATATCAATAATAGTTAGATgcgtgttatttg                                                                                                                     >  7:126013/1‑140 (MQ=18)
                           cACGTTGATATTGCTCGCACTGGGCGAAGGTGGGGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGTGGAAATCAAATATAGTTAGATGAAtgttttttgt                                                                                                                    >  5:220483/1‑144 (MQ=17)
                           cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCACCAAATAACGGTATATCATATAAAGTTTCATGCCtgtggtatcc                                                                                                                    >  3:1424093/1‑145 (MQ=17)
                           cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATCGAGGTTAATTCATAAAATGTTTGATGAgtgttttttg                                                                                                                     >  4:15027/1‑144 (MQ=17)
                              gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAAAGCTAATTCAAATAAAGTTTGATTCCTGTGGGatcgtcc                                                                                                                 >  8:133029/1‑145 (MQ=14)
                                tGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAAGGATAATTAAAAAAAAGTTTGATGAAtgatttttcgtcccg                                                                                                               >  2:554154/1‑138 (MQ=14)
                                 gATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCTAGATCCAATCATGTATGATGACtgtggtttcgtgccgg                                                                                                              >  1:199651/1‑139 (MQ=17)
                                 gATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAAATCTAAATAAAAAAAAGTTAGATGAAtgttatttaatcgcgg                                                                                                              >  8:347730/1‑138 (MQ=14)
                                 gATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGGTAAATCAAATAAAGTAAGATGAAtgagatataatggcgg                                                                                                              >  4:1495725/1‑137 (MQ=14)
                                  tatgttgtgggaCTGGGGGAAGGTGGCGGAATTGGTAGAGGAGCTAGCTGCATGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCgg                                                                                                              <  3:394109/139‑1 (MQ=21)
                                   tatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAACGAGGGGAGATCCATAAAAGTAAGATGAGtgagttttggtgccgga                                                                                                             >  5:1824433/1‑135 (MQ=17)
                                   tatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAAACGAGGGGATTTCCATAAATGTTAGTTGCCTGTGGGTTGCTGGCGGa                                                                                                             >  1:521004/1‑150 (MQ=17)
                                      aactgattctcggggAAGGCGGCGGAATTGGTCGACGCGCTAGCGTCAGGTGGTATTGTTATTACTGACGTGGGGGTGCAACGCCCCCCCCTCGCACGAACCGATCCCATATCAAAAAAAGTAATCTGACTGTGCGAAGGTGGCGGAAtt                                                                                                          <  5:702350/136‑1 (MQ=255)
                                       gCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGTCGCGATATCAAATATTGTTAGTTGAGTGTTGTATGGTGGCGGATTTgg                                                                                                        >  4:1390918/1‑151 (MQ=17)
                                        cTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGGATTTCAAAAAATGTATCATGCATGTGCGATCGTCGCGGattatgt                                                                                                       >  7:212977/1‑145 (MQ=17)
                                          cGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAACTGGGGAGTTCAAATAATGTTTGTTGCCTGGGGGTTGGTGGCGGAATTGGttt                                                                                                     >  7:1712913/1‑149 (MQ=14)
                                           tctgtggggtgaGGGGGCGTAATAGGTAGACGCTCTAGATCGAGGTGTTATTGTTCTTACGGACGTGCTGGTTCAAGGCCCACGCCTCCGACTACACGCTTCGAATTCTATTAAAGTAATATTTCTGTGCGAAGGTGGCGGAATTGGTAg                                                                                                     <  3:1397011/139‑1 (MQ=255)
                                             aCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCACACGAGGGGAGTTCAAAAAAAGTTTGATGACGGTGGGCAGGGGGCGGAGTTGGttgccg                                                                                                  >  3:1211576/1‑146 (MQ=17)
                                                  gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAAATCCATTATAGTTTGTTGACTGTTTTTAGCTGGCGGAATTGATataatattg                                                                                              >  7:166784/1‑142 (MQ=11)
                                                  gCGAAGGGGCGGGTAGTGGTAGACGGGCTTTCTTAAGGTGTCTGTTGGGTTCGGGACGTTGGGGTGAAATGCCCCCCACTCGCACTAACCGCTTCGATATCTTTTAAAGTAATCTTACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTa                                                                                             <  7:1014504/151‑1 (MQ=255)
                                                             ggAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTAAAGTCCCCCCCCTCGCACCAAAAAAAACGATAAAAAAAATAGGAATAGGACAAGGGGAAGGTTGTGGTATAGGGAGAcgcatcgggagagagtgg                                                                                  >  6:467025/1‑136 (MQ=2)
                                                                 ttccgacccgctcgcgCTTCAGTTGTTCGTGTTCTTAGGGACTTGGGGGTGGATGTCCCCCCCCTCGCACCACCCGATGCTATATCAATTAAAGTTAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAg                                                                               <  4:709560/135‑1 (MQ=21)
                                                                       gacgccgtatcgtCAGGTGTTCGTGTTCTTACGGTTGCGCTGGTTCAAGTCCCACCCCTCGCACCACAAGAGGATATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCtt                                                                        <  5:476661/139‑1 (MQ=21)
                                                                       gACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGACGATATATCAAAAAAAGTAAGATGAGTGTTGTAAGGTGGCTGAATTGGTATCATGGATATCTTCATGTGTTAgtgtaaa                                                                         >  5:419822/1‑147 (MQ=17)
                                                                         cgcgCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGGGTATATCAAAAAAAGTTTGATGCCTGTGGGTTGGTGGCGGAATTTGTAGGAGGGGGAGCTTCAGGGGTTATTGTACtt                                                                        >  5:67950/1‑149 (MQ=17)
                                                                            gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACCAGGGTAAATCAATAAAAGTTAGATGCCTGTTGGTTGGTGCCGGAATTTGTATCATGGGGAGCTTCATGTTGTAGTGTCATTACggc                                                                   >  7:739114/1‑150 (MQ=9)
                                                                             cTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGTAAATCATAAATAGTTTGTTGCATGTGGGTTCGTGGCGGAATTGTTTGCAGGGGTATCATCATGTGTTAGTGTACTTAAggcc                                                                  >  5:133892/1‑149 (MQ=9)
                                                                                   tCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGATCAAAAAAAGTTTGATGACTGTTGTTTGGTGGCGGATTTGTTATAAGGTGGATCTTCAGGGGTGAGTGTAATTACGG‑CCgggggg                                                           >  5:658163/1‑151 (MQ=9)
                                                                                    cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATACGGTAAATCAAAAAATGTTTGATGACTGTTTTTAGGTGCCGGAATTAGAATAATGGATATCTTCAGGTTTTAGTGTAATTAAGG‑CCGTcgggg                                                          >  3:614738/1‑151 (MQ=9)
                                                                                     aGGTGTTTGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCACCCGCGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGt                                                         <  7:420076/151‑1 (MQ=255)
                                                                                     aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGTGGGGATATCAAAAAAAGTTTGATGCATGTTATAAGGTGGCGGAATTGTTATACGGGGTAGCTTCAGGTGTTAGTGTCCTGAGGG‑ACGGGGGGGt                                                         >  5:1507143/1‑151 (MQ=21)
                                                                                       gtgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGGATATGAAAAAAATTTAGATGACTGTGGGTTGGTGGCGGATTTGGTTTAAGGGGTAGCGTCATGTTTTAGTGTCTTTACGG‑ACGTGGGGGttt                                                       >  7:307192/1‑150 (MQ=16)
                                                                                       gtgtTAGTGTTCTTACGGACGTGGGGGTTAAAGTCCCCCCCCTCGCACCAAAACAGGCGATATCAAAAAAAGTTAGATGCATGTTGGAAGGTGGCGGAATTTGTTGAAGAGCGATCTTCAGGTTGTACTGTCATTTCGG‑ACGGGCGGGttg                                                       >  7:1266250/1‑150 (MQ=16)
                                                                                         gtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTATGATCACTGTGCGAACGTGGCGGAATTGGTAGACGGGCAAGCGTCAGGTGTTACTGTACATACGG‑ACGTGGGGGttga                                                      >  4:554005/1‑148 (MQ=25)
                                                                                         gtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTAGATGACTGTTTTTTGGTGGCGGAATTTGTATACGGAGAAGCTTCAGGTGTTAGTGTGCTTACGGCACG‑GGGGGttgc                                                      >  5:1810121/1‑148 (MQ=16)
                                                                                          ttAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGATGAAATAAAGTTAGTTGCCTGTTGTTAGGTGGCGGGATTGGTTTAAGGGGAAGATTTAGGTGGTACTGTACTTACCG‑CCCTGGGGGGTcccg                                                    >  1:530013/1‑148 (MQ=9)
                                                                                            aGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAAATCAAAAAAAGTTTGTTGAATGTGGGTTGGTGGCGGAATTTTTATACGGGGTAGCTTCAGGTGTTAGTGTCATTAAGG‑CAGTGGGGGttggagg                                                   >  5:417335/1‑145 (MQ=16)
                                                                                              tgtTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAAAGTTTGTTGAATGTGGTATGGTGGCGGAATTGGTATACGGGGAAGATTCAGGTTTTCGTGTAATAACGG‑CCGTgcggggtgtaggcc                                                 >  1:1154878/1‑141 (MQ=16)
                                                                                              tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGGAAATCAAATAAAGTTAGTTGACTGTGGTTTGGTGCCGGAATTGGTATCCGTGGTAGCTTCATGTTTGAGGGTACTTACGG‑CCGTGGGGGtggtaggac                                                 >  6:999378/1‑142 (MQ=16)
                                                                                                   ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGATCAAAAAAAGTTAGATGACTGTTGTATGGTGGCGGAATTGGTATAAGGGGTATCTTCAGGTTTTACTGTACTTAAGG‑CCGTGGGGGTTCAAGT‑gccacccc                                          >  3:209870/1‑151 (MQ=19)
                                                                                                   ttACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCAAACGAGGGGTTATGAAATAAAGTAAGATGACTGTGGGTAGGTGGCGGGATTGGAATACGCTCTAGGGTCCGGTGTTAGTGTCATTACGG‑ACGTGGGCGTTCCAGG‑ccaaaccc                                          >  1:1713352/1‑144 (MQ=255)
                                                                                                     aCGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGCGATATAAAAAAAAGTAAGATGAATGTTGTAAGGTGGCGGAATTGGTATAAGTGGTATCATCATGTGTTATTGTAATTAAGG‑AAGTGGGGGTTGaatgacaacacaa                                          >  7:533104/1‑139 (MQ=16)
                                                                                                         aCGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGCGAAGGTGGCGGATTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTGGGAGG‑cccccccccagccc                                    >  1:604557/1‑145 (MQ=25)
                                                                                                                    cAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTTAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCTAGG‑CACCCCCCACGCACCAATTATtttt                         >  5:46111/1‑151 (MQ=21)
                                                                                                                    cAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCttt                         >  5:530038/1‑151 (MQ=255)
                                                                                                                    cAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCGAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCttt                         >  5:520945/1‑151 (MQ=255)
                                                                                                                     aaGTCCCCCCCCTCGCACCAAAGGAGGCGATATCAAAAAAAGTAAGATCAATGTTGTAAGGTGGCTGAATAGTAATAATAGTAAGCTTCAGGTTTTAGTGTAGTTACGG‑CAGTGGGGGTTGAAGG‑CACCCCTCTTGCAGCaagtctgtttt                        >  7:1502122/1‑142 (MQ=255)
                                                                                                                         ccccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGGTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTGAAGG‑CCCCCCCCTCGCACCAATTATGTTTACTTc                    >  1:1077485/1‑151 (MQ=255)
                                                                                                                          cccccccTCGCACCAAAGGAGGGGATATCAAAAAAAGTAAGATGACTGTGGGATGGTGGCGGAATTGGTAGACGAGATAGCTTCAGGTGTTAGTGTCGTTACGG‑CCGTGGGGGTTCAAGT‑AACAACCATAGCACCAATTATATTTAATTcc                   >  6:233798/1‑151 (MQ=255)
                                                                                                                          cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATGTTTTCTTcc                   >  5:1770431/1‑151 (MQ=255)
                                                                                                                           ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑CCGTGGGGGGTCCAGT‑CCCCCCCCGCGCCCCCATTTTCTGTACttgcg                  >  7:1166159/1‑148 (MQ=21)
                                                                                                                           ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTtacttact                  >  3:613898/1‑151 (MQ=255)
                                                                                                                           ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑‑CCCCCCCTCGCACCAATTATCTTTACTTCCtt                 >  1:1411559/1‑151 (MQ=255)
                                                                                                                            cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCtt                 >  1:974888/1‑151 (MQ=255)
                                                                                                                            cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCtt                 >  3:1604473/1‑151 (MQ=255)
                                                                                                                            cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCtt                 >  1:811564/1‑151 (MQ=255)
                                                                                                                            cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTAATTACGG‑ACGTGGGGGTTGAAGT‑ACACCCCCTCGCCCCAATTATCTTTACTTCCtt                 >  4:1673531/1‑151 (MQ=25)
                                                                                                                             ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCttt                >  3:1495303/1‑151 (MQ=255)
                                                                                                                              cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTGAAGT‑CCCCCCCCTCGCCCCAATTATATTTACTTACtttt               >  8:1172913/1‑150 (MQ=255)
                                                                                                                              cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGGTCAAGG‑CCCCCCCCGCGCACCAATTATATTTACTTCCtttt               >  1:900153/1‑150 (MQ=255)
                                                                                                                               ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCt              >  1:379801/1‑151 (MQ=255)
                                                                                                                                cTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCtt             >  5:1179657/1‑151 (MQ=255)
                                                                                                                                cTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCGCGCCCCAATTTTCTTTACTTACTTTCtt             >  7:804396/1‑151 (MQ=255)
                                                                                                                                 tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTg            >  3:789116/1‑151 (MQ=255)
                                                                                                                                  cGCCCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTGAAGT‑CCCCCCCCGCGCCCCAATTATCTGTACTGccggtcgtgt           >  6:1681667/1‑143 (MQ=18)
                                                                                                                                  cGCACAAAACGAGGAGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGATAGTGTCCTTACGG‑ACGTTGGGGTTCAAGT‑CCCCCCCAAAGCACCAAATATCTTTAATTAATTTCTTGt           >  2:1725743/1‑151 (MQ=18)
                                                                                                                                   gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCATTACTTCCTTTCTTGtt          >  4:1173294/1‑151 (MQ=255)
                                                                                                                                   gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtt          >  1:414858/1‑151 (MQ=255)
                                                                                                                                   gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGGTCAAGT‑CCCCCCCCTCGCCCCAATTATGTTTAATTCCTTTgttgtt          >  5:690052/1‑151 (MQ=255)
                                                                                                                                    cACCAAACGAGGCGATATCAAAAAAAGTAAGATGAGTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑CCGTGGGGGTTCCAGT‑GCCCACCCTCGCACCACTTTGCTTTAATTCCGTGCTTGttg         >  8:754080/1‑150 (MQ=18)
                                                                                                                                    cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTTTCTTTACTTCCGTTCGTGttt         >  3:559985/1‑151 (MQ=255)
                                                                                                                                    cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACAAATTATCTGTACTTCCTTTCTTGttt         >  5:188558/1‑151 (MQ=255)
                                                                                                                                    cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGG‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCtttttt         >  2:649475/1‑151 (MQ=255)
                                                                                                                                    cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGGTAAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCGTTGtttttt         >  5:444472/1‑151 (MQ=21)
                                                                                                                                      ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATGTTTACTTGCTTTCTTGTttct       >  1:1250717/1‑151 (MQ=255)
                                                                                                                                      ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct       >  1:108205/1‑151 (MQ=255)
                                                                                                                                      ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGCGGGTTCAAGT‑CCCCCCCCGCCACCCAATTTTCTTTACTTCCTTTGTTGtttat       >  3:625645/1‑149 (MQ=21)
                                                                                                                                      ccAAACGACGCGATATCAAAAAAAGTAACATGACTGGGCCCAGCGGGCGGAACTGTGCGCCGCGCTACCTTCAGGTGTTAGTGTGCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct       <  3:457410/151‑1 (MQ=21)
                                                                                                                                       cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTCCGAAGGTGGCGGAATTCGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTGAACT‑ACCCCCCCACGCCCCAAAAATATTTTATTACTTTATTGTttatt      >  7:376967/1‑151 (MQ=17)
                                                                                                                                        aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttc     >  3:76181/1‑151 (MQ=255)
                                                                                                                                         aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTcc    >  8:1171656/1‑151 (MQ=255)
                                                                                                                                         aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTGCCTTTCTTGTttcttac    >  5:678737/1‑149 (MQ=255)
                                                                                                                                           cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGTCCCCCCCCCGCGCCCCCATTATCTTTACGTCCTTTCTTGTTTGTTGCt   >  2:1448789/1‑151 (MQ=21)
                                                                                                                                           cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGGCCCAATTTTCTTTACTTCCTTTGTTTTTTGTTCCtt  >  5:956389/1‑151 (MQ=21)
                                                                                                                                           cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTATTCCtt  >  6:1185190/1‑151 (MQ=255)
                                                                                                                                           cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTAAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATATTTACGTCCTTTCTTGTTTCGTCCtt  >  3:1627425/1‑151 (MQ=255)
                                                                                                                                           |                                                                                                                                                        
TCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGG‑ACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTT  >  DH1/3906693‑3906982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑