Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1477,2650TG68.0% 925.9 / inf 537N195H (AAC→CAC) accCacetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base T (94/77);  new base G (178/186);  total (274/263)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-01

Only 100 of 541 total aligned reads displayed.

CTGGCGCTTCTTCGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCACGGTT  >  DH1/477115‑477415
                                                                                                                                                      |                                                                                                                                                      
gtGGCGCTTCTTCGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGtt                                                                                                                                                        <  6:1516239/150‑1 (MQ=255)
   gcgcTTCTTCGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCt                                                                                                                                                     <  1:1003207/151‑1 (MQ=255)
       ttcttcGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGaa                                                                                                                                                  >  1:1719022/1‑150 (MQ=255)
         cttcGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAagc                                                                                                                                               >  5:937275/1‑151 (MQ=255)
           tcGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAag                                                                                                                                                <  4:93524/148‑1 (MQ=255)
            cGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAagcag                                                                                                                                             <  4:1640123/150‑1 (MQ=255)
             caCCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCt                                                                                                                                           <  1:1375116/150‑1 (MQ=255)
              aCCACTTTCAGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCtt                                                                                                                                          <  3:925054/151‑1 (MQ=255)
                 aCTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCg                                                                                                                                       >  8:947039/1‑151 (MQ=255)
                  cTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGCAAGCAGCTTTCGc                                                                                                                                      >  4:396192/1‑151 (MQ=255)
                   tttCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCt                                                                                                                                     >  4:323804/1‑151 (MQ=255)
                    ttCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGc                                                                                                                                      <  8:1627875/149‑1 (MQ=255)
                        ggtggCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTc                                                                                                                                   <  6:979763/148‑1 (MQ=255)
                        ggtggCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCg                                                                                                                                 <  2:1201410/150‑1 (MQ=255)
                         gtggCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGcc                                                                                                                               >  2:1528331/1‑150 (MQ=255)
                          gggcggcGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCAc                                                                                                                              <  1:286231/150‑1 (MQ=255)
                           ggcggcGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACg                                                                                                                             <  4:197858/151‑1 (MQ=255)
                           ggcggcGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCAc                                                                                                                              <  8:135063/150‑1 (MQ=255)
                           ggcggcGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACg                                                                                                                             >  5:1810305/1‑151 (MQ=255)
                             cggcgTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACggg                                                                                                                           >  1:635992/1‑151 (MQ=255)
                               gcgTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTc                                                                                                                         <  3:786951/151‑1 (MQ=255)
                                cgTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCgacagggtc                                                                                                                         >  6:426663/1‑142 (MQ=255)
                                 gTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCAt                                                                                                                       >  1:930018/1‑151 (MQ=255)
                                   tGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATgg                                                                                                                     >  3:1475193/1‑151 (MQ=255)
                                   tGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTCAAAGCAGCTTTCCCTTCCGCACGGGTCATgg                                                                                                                     >  2:1415322/1‑151 (MQ=255)
                                    gCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGga                                                                                                                    <  6:1275110/151‑1 (MQ=255)
                                    gCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGga                                                                                                                    <  4:1025467/151‑1 (MQ=255)
                                    gCATCGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTCAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCAggg                                                                                                                     >  6:823966/1‑150 (MQ=255)
                                      aTGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATgg                                                                                                                     >  2:1675040/1‑148 (MQ=255)
                                        ggAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGAGCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGTGAt                                                                                                                 <  8:467356/150‑1 (MQ=255)
                                        ggAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTT‑CTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATgg                                                                                                               >  4:577275/1‑151 (MQ=255)
                                        ggAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGAt                                                                                                                 <  2:953711/150‑1 (MQ=255)
                                        ggAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATg                                                                                                                <  7:439868/151‑1 (MQ=255)
                                            cAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGAtt                                                                                                            >  7:1205018/1‑151 (MQ=255)
                                             aGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATtg                                                                                                           >  7:1385009/1‑151 (MQ=255)
                                              gTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATtgt                                                                                                          <  5:1105911/151‑1 (MQ=255)
                                               tCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATtgtg                                                                                                         >  6:1645234/1‑151 (MQ=255)
                                                cACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGc                                                                                                        >  7:222930/1‑151 (MQ=255)
                                                   gTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAg                                                                                                     <  7:555956/151‑1 (MQ=255)
                                                    ttCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGt                                                                                                    >  7:1505302/1‑151 (MQ=255)
                                                      ccGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTc                                                                                                  <  7:855483/151‑1 (MQ=255)
                                                      ccGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTc                                                                                                  <  3:137319/151‑1 (MQ=255)
                                                         ccAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGc                                                                                               <  5:540995/151‑1 (MQ=255)
                                                           agatagatAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGt                                                                                             >  2:1115820/1‑151 (MQ=255)
                                                            gatagataGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTc                                                                                            >  4:1564674/1‑151 (MQ=255)
                                                             atagatagCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTcg                                                                                           <  4:1277145/151‑1 (MQ=255)
                                                               agatagCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTcgcc                                                                                         <  3:102532/151‑1 (MQ=255)
                                                                     cGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCAc                                                                                   <  7:1310626/151‑1 (MQ=255)
                                                                     cGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCAc                                                                                   <  8:1839560/151‑1 (MQ=255)
                                                                     cGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGATTTCGCTTCCGCACGGGTCATCGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCAc                                                                                   >  4:1415051/1‑151 (MQ=255)
                                                                      gTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACt                                                                                  <  1:1520777/151‑1 (MQ=255)
                                                                      gTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACt                                                                                  >  4:883360/1‑151 (MQ=255)
                                                                      gTTGCCCAGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCAAGGTATTTCTCCATCTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGCTCATGGAGATGGATTGAGCCACTTCAGCGTCGCCGCGCACt                                                                                  >  1:1096281/1‑151 (MQ=255)
                                                                           ccTGACCGTCAGCCAGTACCTGAATCTCGACGTGGAGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTAcgcg                                                                             >  8:1417553/1‑151 (MQ=255)
                                                                             tGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACgcgc                                                                            >  8:635643/1‑150 (MQ=255)
                                                                                 cGTCAGCCAGTACCTGAATCTCGACGTGGCGTGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATAcc                                                                       <  2:483044/151‑1 (MQ=255)
                                                                                 cGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATAcc                                                                       <  5:1473164/151‑1 (MQ=255)
                                                                                 cGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATAcc                                                                       <  7:969575/151‑1 (MQ=255)
                                                                                 cGTCAGCCAGTACCTGAATCTCGACCTGGCGACGATTTTCCAGCTATTTCTCCATGTAAACCATATCGACGCTGAAAGCACCTTTCGCTTACGCACGGCTCAAGGAGATGGATTGTGCCAGTTCAGCGTCGACGCCCACTACGCGCATAcc                                                                       >  4:1521206/1‑151 (MQ=255)
                                                                                  gTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACcg                                                                      >  5:42479/1‑151 (MQ=255)
                                                                                  gTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACcg                                                                      <  5:654237/151‑1 (MQ=255)
                                                                                            aCCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGAccgccgc                                                            >  8:1771494/1‑151 (MQ=255)
                                                                                                gAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGAccgccgccgcc                                                        >  2:1791052/1‑151 (MQ=255)
                                                                                                  aTCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGAcctccgccgccgc                                                      >  5:24968/1‑151 (MQ=255)
                                                                                                  aTCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGAccgccgccgccgc                                                      >  3:1638359/1‑151 (MQ=255)
                                                                                                      cGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCgg                                                   >  5:823446/1‑150 (MQ=255)
                                                                                                      cGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCgg                                                   >  5:1041114/1‑150 (MQ=255)
                                                                                                      cGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGa                                                  <  1:347379/151‑1 (MQ=255)
                                                                                                       gACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAg                                                 <  2:320798/151‑1 (MQ=255)
                                                                                                        aCGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAgg                                                <  3:439372/151‑1 (MQ=255)
                                                                                                          gTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCt                                              >  6:649096/1‑151 (MQ=255)
                                                                                                          gTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCt                                              <  4:1454968/151‑1 (MQ=255)
                                                                                                           tGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCtt                                             >  3:1221732/1‑151 (MQ=255)
                                                                                                           tGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCtt                                             >  8:1091753/1‑151 (MQ=255)
                                                                                                           gggCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCtt                                             <  8:1574423/150‑1 (MQ=255)
                                                                                                             gCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTg                                           >  3:20254/1‑151 (MQ=255)
                                                                                                                   aTTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATc                                     <  6:1084996/151‑1 (MQ=255)
                                                                                                                   aTTTTCCAGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATc                                     <  3:1096117/151‑1 (MQ=255)
                                                                                                                      ttCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCAcc                                  >  5:1325939/1‑151 (MQ=255)
                                                                                                                       tCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCAcc                                  <  7:1761211/150‑1 (MQ=255)
                                                                                                                       tCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCAcc                                  <  5:1577522/150‑1 (MQ=255)
                                                                                                                        ccAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCgg                                >  7:1621829/1‑151 (MQ=255)
                                                                                                                         cAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGA‑‑‑CCGCCGCCGCCGGAGGCTTTGATAATCACCGGATa                             >  4:675292/1‑150 (MQ=255)
                                                                                                                          aGGTATTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGAt                              <  8:1221313/151‑1 (MQ=255)
                                                                                                                              aTTTCTCCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAAc                           <  1:1182763/150‑1 (MQ=255)
                                                                                                                                 tctcCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATACCCAAt                       >  3:594219/1‑151 (MQ=255)
                                                                                                                                  ctcCATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATg                      >  1:803185/1‑151 (MQ=255)
                                                                                                                                    ccATGTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGc                     <  7:360501/150‑1 (MQ=255)
                                                                                                                                       tGTCAACCATATCGTGGCTGAAAGCAGCTTGCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGtt                  <  2:423202/150‑1 (MQ=255)
                                                                                                                                        gTAAACCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGttt                 >  7:660564/1‑150 (MQ=255)
                                                                                                                                          aaaCCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGc              >  1:495438/1‑151 (MQ=255)
                                                                                                                                            aCCATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCaa            <  6:1741120/151‑1 (MQ=255)
                                                                                                                                              cATATCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATg          <  5:1721511/151‑1 (MQ=255)
                                                                                                                                                 atCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGCTAATCACCGGATAACCAATGCGTTTAGCAATGGcc       >  2:637038/1‑150 (MQ=255)
                                                                                                                                                 atCGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGGCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCa       <  6:324893/151‑1 (MQ=255)
                                                                                                                                                   cGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCACg     >  7:1102030/1‑151 (MQ=255)
                                                                                                                                                   cGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCAc      <  2:833291/150‑1 (MQ=255)
                                                                                                                                                   cGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCACg     >  3:290818/1‑151 (MQ=255)
                                                                                                                                                   cGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCACg     >  8:826464/1‑151 (MQ=255)
                                                                                                                                                   cGTGGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCACg     <  4:293967/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CTGGCGCTTCTTCGACCACTTTCTGGTGGCGGCGTTGCATGGAGCAGTCACGTTCCGCCAGATAGATAGCGTTGCCCTGACCGTCAGCCAGTACCTGAATCTCGACGTGGCGAGGATTTTCCAGGTATTTCTCCATGTAAACCATATCGTTGCTGAAAGCAGCTTTCGCTTCCGCACGGGTCATGGAGATGGATTGTGCCAGTTCAGCGTCGCCGCGCACTACGCGCATACCGCGACCGCCGCCGCCGCCGGAGGCTTTGATAATCACCGGATAACCAATGCGTTTAGCAATGGCACGGTT  >  DH1/477115‑477415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑