Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,820,579 G→A L207F (CTT→TTT)  yeeN ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,820,5790GA98.0% 1627.9 / 11.3 451L207F (CTT→TTT) yeeNtranscriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base A (234/207);  total (241/210)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 451 total aligned reads displayed.

ATATGAGATTAAAGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAGCTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTCACATCACGAA  >  DH1/1820431‑1820725
                                                                                                                                                    |                                                                                                                                                  
atatGAGATTAAAGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACt                                                                                                                                                  >  4:1554442/1‑151 (MQ=255)
atatGAGATTAAAGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACt                                                                                                                                                  >  4:421187/1‑151 (MQ=255)
    gagaTTAAAGATATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCaa                                                                                                                                               <  7:1093438/150‑1 (MQ=255)
     agaTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACt                                                                                                                                             >  4:1792882/1‑151 (MQ=255)
       aTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGCCGTTATGATAAACTTTTTGTACATAGTCGTCATCTTCAAGGGCATCAACATGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTc                                                                                                                                           >  3:870755/1‑151 (MQ=255)
          aaaGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAg                                                                                                                                         <  3:250621/150‑1 (MQ=255)
          aaaGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAg                                                                                                                                         <  5:413624/150‑1 (MQ=255)
           aaGAAATCTGGCGTTATTACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGCCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTGAGAt                                                                                                                                       <  2:939887/151‑1 (MQ=255)
           aaGAAATCTGCCTTAAAGACAGATTTCTTTAAAAGATAATTATAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGAt                                                                                                                                       <  8:521088/151‑1 (MQ=255)
             gAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTg                                                                                                                                     <  1:825043/151‑1 (MQ=255)
                    gCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCaa                                                                                                                                <  6:1070983/149‑1 (MQ=255)
                    gCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCa                                                                                                                                 <  8:1803726/148‑1 (MQ=255)
                    gCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATc                                                                                                                              <  5:1544760/151‑1 (MQ=255)
                        tAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCAtt                                                                                                                           <  8:726680/150‑1 (MQ=255)
                         aaaGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTACTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTc                                                                                                                         >  2:575855/1‑151 (MQ=255)
                          aaGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCt                                                                                                                        >  7:60611/1‑151 (MQ=255)
                           aGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCt                                                                                                                        >  8:1344642/1‑150 (MQ=255)
                             aCAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAATATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTTAGAAATCATTTCTAAt                                                                                                                     <  2:1476917/151‑1 (MQ=255)
                             aCAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAGCTCAACTTCAGATTGAGCAATCATTTCTAAt                                                                                                                     >  7:1519233/1‑151 (MQ=255)
                             aCAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAAt                                                                                                                     <  6:1132024/151‑1 (MQ=255)
                               aGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTc                                                                                                                   <  2:286356/151‑1 (MQ=255)
                               aGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTc                                                                                                                   <  7:319284/151‑1 (MQ=255)
                                gATTTCGTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCt                                                                                                                  <  5:545018/151‑1 (MQ=255)
                                   ttCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCtgtt                                                                                                               >  2:1030239/1‑151 (MQ=255)
                                    tCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCtgtt                                                                                                               <  4:211360/150‑1 (MQ=255)
                                    tCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCtgtt                                                                                                               <  1:965496/150‑1 (MQ=255)
                                    tCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTAtgttg                                                                                                              >  3:1250540/1‑151 (MQ=255)
                                     cTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCtgttgt                                                                                                             >  8:1752891/1‑151 (MQ=255)
                                     cTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCtgttgt                                                                                                             >  8:402582/1‑151 (MQ=255)
                                      tttAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTgttgtt                                                                                                            >  7:1690984/1‑151 (MQ=255)
                                        tAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGttgttg                                                                                                           >  3:1749753/1‑150 (MQ=255)
                                             gATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACt                                                                                                     >  3:806859/1‑151 (MQ=255)
                                              aTAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTc                                                                                                    <  1:577921/151‑1 (MQ=255)
                                               tAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAGCTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTc                                                                                                    >  1:1214659/1‑150 (MQ=255)
                                                aaTTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTc                                                                                                    >  7:140746/1‑149 (MQ=255)
                                                 aTTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGt                                                                                                 <  1:1595929/151‑1 (MQ=255)
                                                   tAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTg                                                                                                <  4:931885/150‑1 (MQ=255)
                                                   tAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTCCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTg                                                                                                >  6:1278020/1‑150 (MQ=255)
                                                    agagATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGAt                                                                                              <  2:892767/151‑1 (MQ=255)
                                                       gaTTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTcc                                                                                           <  4:813641/151‑1 (MQ=255)
                                                        aTTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTcc                                                                                           >  3:1069622/1‑150 (MQ=255)
                                                        aTTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCa                                                                                          <  5:690924/151‑1 (MQ=255)
                                                           tGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAgct                                                                                       >  4:1326204/1‑151 (MQ=255)
                                                           tGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAgc                                                                                        <  4:1085038/150‑1 (MQ=255)
                                                                 gTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCtttt                                                                                 >  2:1408116/1‑151 (MQ=255)
                                                                   tATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCtttt                                                                                 >  7:869119/1‑149 (MQ=255)
                                                                    aTGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTaga                                                                              <  4:1198967/151‑1 (MQ=255)
                                                                           aCTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGa                                                                       <  7:43570/151‑1 (MQ=255)
                                                                            cTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGAt                                                                      >  6:1190762/1‑151 (MQ=255)
                                                                            cTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGAt                                                                      >  5:927406/1‑151 (MQ=255)
                                                                                ttGTACATCGTCGTCATCTTCAAGTGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTcc                                                                   <  7:1034404/150‑1 (MQ=255)
                                                                                ttGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCt                                                                  >  4:505791/1‑151 (MQ=255)
                                                                                  gTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCGTCAAAGATTTCTAAATCTTCAGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCtat                                                                >  6:681932/1‑149 (MQ=255)
                                                                                   tACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCttt                                                                >  3:973247/1‑150 (MQ=255)
                                                                                   tACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCttt                                                                <  5:1087274/150‑1 (MQ=255)
                                                                                      aTCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGa                                                            <  7:1229250/151‑1 (MQ=255)
                                                                                         gtcgtcATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAgg                                                         >  4:732677/1‑151 (MQ=255)
                                                                                         gtcgtcATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAgg                                                         >  8:1580890/1‑151 (MQ=255)
                                                                                          tcgtcATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGt                                                        >  4:307223/1‑151 (MQ=255)
                                                                                           cgtcATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGt                                                        <  8:1453253/150‑1 (MQ=255)
                                                                                           cgtcATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTc                                                       <  8:315105/151‑1 (MQ=255)
                                                                                            gtcATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCa                                                      <  3:419996/151‑1 (MQ=255)
                                                                                              cATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAg                                                     <  4:62837/150‑1 (MQ=255)
                                                                                              cATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGt                                                    >  7:945064/1‑151 (MQ=255)
                                                                                               aTCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGt                                                    >  6:425379/1‑150 (MQ=255)
                                                                                                  ttCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGt                                                >  2:715457/1‑151 (MQ=255)
                                                                                                   tCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAgg                                                 <  8:1227383/149‑1 (MQ=255)
                                                                                                    cAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGtt                                               <  3:133436/150‑1 (MQ=255)
                                                                                                     aaGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTc                                              >  5:1747174/1‑150 (MQ=255)
                                                                                                         gCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGtat                                         <  6:216427/151‑1 (MQ=255)
                                                                                                            tCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTataa                                       >  5:1005462/1‑150 (MQ=255)
                                                                                                             caacaaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTataaat                                     >  5:643089/1‑151 (MQ=255)
                                                                                                              aacaaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTataaat                                     >  1:715492/1‑150 (MQ=255)
                                                                                                               acaaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTataaata                                    >  5:287947/1‑150 (MQ=255)
                                                                                                               acaaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAATGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTataaataa                                   <  8:905802/151‑1 (MQ=255)
                                                                                                                caaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAAc                                  <  3:1534741/151‑1 (MQ=255)
                                                                                                                 aaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAGCTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGcc                                                                           <  1:1764431/109‑1 (MQ=255)
                                                                                                                 aaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAGCTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGcc                                                                           >  2:1767877/1‑109 (MQ=255)
                                                                                                                 aaGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACa                                 <  5:22462/151‑1 (MQ=255)
                                                                                                                  aGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACaa                                <  3:644357/151‑1 (MQ=255)
                                                                                                                   gCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAAt                               >  1:2172/1‑151 (MQ=255)
                                                                                                                   gCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTATAGAGCCGCGATTCCTTTATCAACGTCAGTAGGTTCAGTATAAATAACaa                                >  2:1297148/1‑150 (MQ=255)
                                                                                                                     ccTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGt                             <  1:399060/151‑1 (MQ=255)
                                                                                                                     ccTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGt                             <  7:1001452/151‑1 (MQ=255)
                                                                                                                      cTTCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCCAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGCCAGTAGGTTCAGTATAAATAACAATGtt                            >  3:1160702/1‑151 (MQ=255)
                                                                                                                       ttCAAAGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGtt                            >  6:1821612/1‑150 (MQ=255)
                                                                                                                          aaaGATTTCTATATCTTCTGTGGAAAACTCAACTTGAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTGAGTGATTCGAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACct                        <  2:1197802/151‑1 (MQ=255)
                                                                                                                            aGATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACcttg                      >  5:1684829/1‑150 (MQ=255)
                                                                                                                             gATTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACcttct                     <  4:1460513/151‑1 (MQ=255)
                                                                                                                              aTTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACcttctt                    <  5:1202220/151‑1 (MQ=255)
                                                                                                                              aTTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACcttctt                    <  1:90616/151‑1 (MQ=255)
                                                                                                                              aTTTCTAAATCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACcttctt                    <  5:871893/151‑1 (MQ=255)
                                                                                                                                     aaTCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTGTTCAGt             >  6:1644255/1‑151 (MQ=255)
                                                                                                                                     aaTCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGt             >  2:1561894/1‑151 (MQ=255)
                                                                                                                                      aTCTTCTGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTc            >  1:1329390/1‑151 (MQ=255)
                                                                                                                                          tctGTGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTca           <  4:875077/148‑1 (MQ=255)
                                                                                                                                          tctGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTca           <  1:1029368/148‑1 (MQ=255)
                                                                                                                                          tctGGGGAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTc            <  6:1734254/147‑1 (MQ=255)
                                                                                                                                               ggAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGCTACCTTCTTCTTCAGTCACATCACg    >  3:1618675/1‑150 (MQ=255)
                                                                                                                                                gAAAACTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTCACATCACGaa  <  5:1445385/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATATGAGATTAAAGAAATCTGCCGTAAAGACAGATTTCTTTAAAAGATAATTAGAGATTTGCGACGTTATGATAAACTTTTTGTACATCGTCGTCATCTTCAAGGGCATCAACAAGCCCTTCAAAGATTTCTAAATCTTCTGGGGAAAGCTCAACTTCAGATTGAGCAATCATTTCTAATTCTGTTGTTGAGAACTCAGTGATTCCAGCTGCTTTTAGAGCCGCGATTCCTTTATGAAGGTCAGTAGGTTCAGTATAAATAACAATGTTACCTTCTTCTTCAGTCACATCACGAA  >  DH1/1820431‑1820725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: