Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,985,603 G→T D121Y (GAT→TAT)  yoaA → ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,985,6030GT98.9% 1320.6 / ‑1.2 354D121Y (GAT→TAT) yoaAATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (190/160);  total (194/160)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

CTCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCTTG  >  DH1/1985455‑1985750
                                                                                                                                                    |                                                                                                                                                   
cTCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTAt                                                                                                                                                   >  2:1110535/1‑151 (MQ=255)
 tCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATc                                                                                                                                                  >  7:592317/1‑151 (MQ=255)
  cAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCt                                                                                                                                                 <  1:109252/151‑1 (MQ=255)
  cAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCt                                                                                                                                                 >  7:350274/1‑151 (MQ=255)
   aaaaGCGTTGCAGGCTCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGGCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTCTCTg                                                                                                                                                >  7:466974/1‑151 (MQ=255)
   aaaaGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTg                                                                                                                                                <  1:385049/151‑1 (MQ=255)
   aaaaGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTg                                                                                                                                                >  8:49123/1‑151 (MQ=255)
   aaaaGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTg                                                                                                                                                <  7:1648057/151‑1 (MQ=255)
     aaGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGcc                                                                                                                                              >  1:283566/1‑151 (MQ=255)
         gTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGt                                                                                                                                           >  3:49026/1‑150 (MQ=255)
           tGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTAc                                                                                                                                         >  5:1143858/1‑150 (MQ=255)
              aGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAAt                                                                                                                                     <  7:1502136/151‑1 (MQ=255)
              aGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCGCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAAt                                                                                                                                     <  3:1350386/151‑1 (MQ=255)
                gATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCt                                                                                                                                   >  1:1613400/1‑151 (MQ=255)
                 aTCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCtt                                                                                                                                  >  1:90074/1‑151 (MQ=255)
                     gCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCATAAGc                                                                                                                              >  5:530021/1‑151 (MQ=255)
                      ctctACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCg                                                                                                                             >  8:129432/1‑151 (MQ=255)
                       tctACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGCACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGGCGGTACAAATCTTAAgggc                                                                                                                            >  6:1189471/1‑148 (MQ=255)
                       tctACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCg                                                                                                                             >  5:471579/1‑150 (MQ=255)
                          agaGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGa                                                                                                                            <  6:75403/146‑1 (MQ=255)
                          aCAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGAtgt                                                                                                                         <  1:747650/151‑1 (MQ=255)
                             gCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGAt                                                                                                                      <  6:374071/151‑1 (MQ=255)
                               cgcgATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATcc                                                                                                                    <  2:1257610/151‑1 (MQ=255)
                                gcgcTTTGCCAAGAGTCTCAAAGGGAGTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAAGTCCCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGTGGCTTTCTGGCGGTACAAATCTTAAGCGATGTGATcgt                                                                                                                   <  8:1551082/151‑3 (MQ=255)
                                gcgATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCct                                                                                                                   >  5:295553/1‑151 (MQ=255)
                                gcgATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCct                                                                                                                   <  7:1806913/151‑1 (MQ=255)
                                 cgATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCctg                                                                                                                  <  4:1189885/151‑1 (MQ=255)
                                      tcccAACAGGCTCAAAGGCATTGAAATATCCGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCtgctgc                                                                                                              <  6:1132384/148‑1 (MQ=255)
                                      tGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTgcg                                                                                                             >  3:1574981/1‑151 (MQ=255)
                                         cAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTc                                                                                                          >  6:1317232/1‑151 (MQ=255)
                                           aCAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCt                                                                                                        >  7:19011/1‑151 (MQ=255)
                                            cAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTg                                                                                                       <  4:1746955/151‑1 (MQ=255)
                                                 tcAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCt                                                                                                   >  4:1653304/1‑150 (MQ=255)
                                                  cAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTc                                                                                                    <  6:1446455/148‑1 (MQ=255)
                                                    aaGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATc                                                                                               <  5:1383639/151‑1 (MQ=255)
                                                    aaGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATc                                                                                               >  4:1366408/1‑151 (MQ=255)
                                                       gCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCaa                                                                                             >  4:930197/1‑150 (MQ=255)
                                                        cATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAAc                                                                                           >  7:1249818/1‑151 (MQ=255)
                                                          ttGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAAc                                                                                           >  5:889578/1‑149 (MQ=255)
                                                            gAAATATAGGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGTGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGt                                                                                        <  3:619462/150‑1 (MQ=255)
                                                            gAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTc                                                                                       >  6:141799/1‑151 (MQ=255)
                                                             aaaTATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCg                                                                                      >  5:1038473/1‑151 (MQ=255)
                                                              aaTATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCg                                                                                      >  3:1486981/1‑150 (MQ=255)
                                                               atatACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGAt                                                                                    >  7:1413597/1‑151 (MQ=255)
                                                               atatACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGAt                                                                                    <  5:161050/151‑1 (MQ=255)
                                                                tataCGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATg                                                                                   >  6:277845/1‑151 (MQ=255)
                                                                 ataCGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATgg                                                                                  >  5:70448/1‑151 (MQ=255)
                                                                  taCGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGt                                                                                 >  2:438248/1‑151 (MQ=255)
                                                                   aCGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTg                                                                                <  8:1062013/151‑1 (MQ=255)
                                                                      ggCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCCGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGata                                                                             <  6:67652/151‑1 (MQ=255)
                                                                       gCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGatat                                                                            >  2:570394/1‑151 (MQ=255)
                                                                         aaCGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATc                                                                           >  1:1282633/1‑150 (MQ=255)
                                                                         aaCGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATc                                                                           >  8:57384/1‑150 (MQ=255)
                                                                         aaCGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATc                                                                           >  2:1369166/1‑150 (MQ=255)
                                                                           cGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGCGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCATGGTCTAATCAAACAGTCGATGGTGATATCAGc                                                                        >  2:1814226/1‑151 (MQ=255)
                                                                            gTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGc                                                                        >  3:750587/1‑150 (MQ=255)
                                                                                  ctgctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCg                                                                 >  4:336355/1‑151 (MQ=255)
                                                                                      tgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               >  5:154291/1‑149 (MQ=255)
                                                                                      tgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               >  8:1583490/1‑149 (MQ=255)
                                                                                       gAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGc                                                            <  2:284356/151‑1 (MQ=255)
                                                                                         aaGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGt                                                          >  6:487300/1‑151 (MQ=255)
                                                                                         aaGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGt                                                          <  8:349054/151‑1 (MQ=255)
                                                                                             gcgcTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCg                                                      <  2:969243/151‑1 (MQ=255)
                                                                                              cgcTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTgcttt                                                     <  1:1396265/151‑5 (MQ=255)
                                                                                                 tCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGa                                                    >  7:310697/1‑149 (MQ=255)
                                                                                                  cAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGa                                                    >  4:814653/1‑148 (MQ=255)
                                                                                                  cAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGa                                                 <  2:782018/151‑1 (MQ=255)
                                                                                                  cAAACTACCTCTGCCTCGAACGTCTCCAACACCAGGCGCTGGCGGCGGCCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCAGCTCCGCTCCTGCTCTAATCAAACAGTCGAAGGTGATATCAGCAACTGCGTCAGCGTGGCGGa                                                    >  8:822310/1‑148 (MQ=255)
                                                                                                   aaaCTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGa                                                    >  3:1116935/1‑147 (MQ=255)
                                                                                                   aaaCTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGAt                                                <  4:140627/151‑1 (MQ=255)
                                                                                                        aCCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTcaca                                           <  3:465100/151‑1 (MQ=255)
                                                                                                           tctGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGc                                        >  7:815869/1‑151 (MQ=255)
                                                                                                            ctGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAgg                                         <  3:154071/149‑1 (MQ=255)
                                                                                                             tGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGGCAGCGTGGCGGAAGATTCACAGGCGt                                      >  6:1725006/1‑151 (MQ=255)
                                                                                                              gCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTg                                     >  3:339064/1‑151 (MQ=255)
                                                                                                                cTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGc                                   >  5:1648724/1‑151 (MQ=255)
                                                                                                                  cGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCg                                 >  3:825677/1‑151 (MQ=255)
                                                                                                                   gAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGc                                >  1:819255/1‑151 (MQ=255)
                                                                                                                   gAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGc                                >  6:930572/1‑151 (MQ=255)
                                                                                                                   gAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGc                                >  8:1209614/1‑151 (MQ=255)
                                                                                                                    aaCGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCt                               >  5:1820112/1‑151 (MQ=255)
                                                                                                                    aaCGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCt                               <  2:104527/151‑1 (MQ=255)
                                                                                                                      cGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTgg                             >  8:1809203/1‑151 (MQ=255)
                                                                                                                        gctcGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTc                           <  1:1145677/150‑1 (MQ=255)
                                                                                                                          tcGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATCGTGATATCAGCACCTGCGTCCGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCAc                         >  4:1053998/1‑151 (MQ=255)
                                                                                                                             aaCAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAg                      <  4:1264276/151‑1 (MQ=255)
                                                                                                                              aCAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGc                     >  7:1749100/1‑151 (MQ=255)
                                                                                                                                agcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAc                   >  2:1715467/1‑151 (MQ=255)
                                                                                                                                agcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAc                   >  4:572462/1‑151 (MQ=255)
                                                                                                                                 gcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAcc                  <  2:1536006/151‑1 (MQ=255)
                                                                                                                                 gcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAcc                  <  1:500460/151‑1 (MQ=255)
                                                                                                                                      cgcTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACg              >  3:225774/1‑150 (MQ=255)
                                                                                                                                      cgcTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACg              >  4:180941/1‑150 (MQ=255)
                                                                                                                                      cgcTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGa             <  7:959858/151‑1 (MQ=255)
                                                                                                                                        cTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGAc            >  1:602872/1‑150 (MQ=255)
                                                                                                                                           gCGTGGGGCTATCTACCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGGCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACt        <  3:564475/151‑1 (MQ=255)
                                                                                                                                              gggggCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTc     >  8:1167352/1‑151 (MQ=255)
                                                                                                                                                gggCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCtt   >  8:1347140/1‑151 (MQ=255)
                                                                                                                                                gggCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCtt   >  6:1176216/1‑151 (MQ=255)
                                                                                                                                                gggCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGAGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCtt   <  1:172331/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCTTG  >  DH1/1985455‑1985750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑