BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	DH1	2,509,217	G→A	S82L (TCA→TTA)	ycjM ←	alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,509,217	0	G	A	96.6%	1337.0 / 20.4	380	S82L (TCA→TTA)	ycjM	alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base G (4/9); new base A (182/185); total (186/194)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 391 total aligned reads displayed.

GCAGACAAAATCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTGAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGTAATTAATCGTT  >  DH1/2509069‑2509364

 cAGACAAAATCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTaaaa                                                                                                                                                  <  6:406059/151‑1 (MQ=255)

 cAGACAAAATCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTaaa                                                                                                                                                   >  8:42242/1‑150 (MQ=255)

  aGACAAAATCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTaaaa                                                                                                                                                  >  7:1714405/1‑150 (MQ=255)

     cAAAATCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAAt                                                                                                                                              >  7:1547106/1‑151 (MQ=255)

     cAAAATCAAACATTAAATGAATGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAAt                                                                                                                                              <  5:885780/151‑1 (MQ=255)

       aaaTCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGc                                                                                                                                            >  8:312152/1‑151 (MQ=255)

        aaTCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCt                                                                                                                                           <  1:366494/151‑1 (MQ=255)

           cAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTg                                                                                                                                        >  3:1510003/1‑151 (MQ=255)

             aaCATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAATCCATCATAAGATGACCATGGATAAAACGGCAAAAAATGAACATGTAAAAAAATGCTTTGCa                                                                                                                                      <  3:1742847/151‑1 (MQ=255)

             aaCATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCa                                                                                                                                      <  8:506365/151‑1 (MQ=255)

             aaCATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCa                                                                                                                                      <  3:405718/151‑1 (MQ=255)

              aCATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAg                                                                                                                                     <  6:1127028/151‑1 (MQ=255)

               cATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGc                                                                                                                                    <  4:687071/151‑1 (MQ=255)

               cATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGACATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGc                                                                                                                                    <  8:839594/151‑1 (MQ=255)

                aTTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGcc                                                                                                                                   >  3:1247288/1‑151 (MQ=255)

                aTTAAATGACTGCATTCACAGAGTTGCTGAATATCCTGCCACTCGCCCGCTTCACTGGCGACGTGATGATTATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGcc                                                                                                                                   <  5:146581/151‑1 (MQ=255)

                 ttAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGcc                                                                                                                                   >  5:681428/1‑150 (MQ=255)

                       gACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCAatgatg                                                                                                                            >  2:807933/1‑151 (MQ=255)

                       gACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGAt                                                                                                                             <  1:1008967/150‑1 (MQ=255)

                       gACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGAAGACCATGGATAAAACGGCAACAAATGAACATGTGAAAAAATGCTTTGCAGCCATTGATg                                                                                                                            >  6:773290/1‑151 (MQ=255)

                           gCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGt                                                                                                                          <  4:1097166/149‑1 (MQ=255)

                             aTTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTaaa                                                                                                                       >  5:1304675/1‑150 (MQ=255)

                             aTTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTaaa                                                                                                                       >  7:798141/1‑150 (MQ=255)

                                cACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTg                                                                                                                   >  2:1592214/1‑151 (MQ=255)

                                 aCCCAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGa                                                                                                                  <  6:1574954/151‑1 (MQ=255)

                                    gAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTa                                                                                                               <  5:554538/151‑1 (MQ=255)

                                     aGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCAGGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTa                                                                                                               >  5:984295/1‑150 (MQ=255)

                                       ttGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAAt                                                                                                            >  2:674723/1‑151 (MQ=255)

                                        tGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAAt                                                                                                            <  2:113260/150‑1 (MQ=255)

                                            gAATATCCTGCCACTCCCCCGCTTCACTGGTGACCTGATGATAATCAATTACCGAAAATCCATCATCAGATGACAATCGATAAAACGGAAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGAAGGTAAAACTCATTAAATGTggg                                                                                                       >  2:1530570/1‑151 (MQ=255)

                                                atCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAAt                                                                                                   >  8:1528153/1‑151 (MQ=255)

                                                atCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACCGCAACAAATGAACATGTAAAAAAATGCTTTCCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAAt                                                                                                   >  2:1254883/1‑151 (MQ=255)

                                                  ccTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATgg                                                                                                 >  4:1621427/1‑151 (MQ=255)

                                                          accccccCTTCACTGGCGACCTGATGATAATCAATTACCCAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAAt                                                                                         <  4:416540/150‑1 (MQ=255)

                                                           cccccGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAAt                                                                                         <  5:1608613/150‑1 (MQ=255)

                                                            ccccGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATc                                                                                        >  2:443776/1‑150 (MQ=255)

                                                            ccccGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCa                                                                                       <  8:724770/151‑1 (MQ=255)

                                                            ccccGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCa                                                                                       <  8:136380/151‑1 (MQ=255)

                                                             cccGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCAt                                                                                      >  4:328846/1‑151 (MQ=255)

                                                             cccGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCAt                                                                                      >  2:210353/1‑151 (MQ=255)

                                                               cGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTg                                                                                    <  8:1061203/151‑1 (MQ=255)

                                                                 cTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCt                                                                                  >  7:886036/1‑151 (MQ=255)

                                                                  ttCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCtg                                                                                 >  1:555664/1‑151 (MQ=255)

                                                                    cACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCtgtg                                                                               >  3:841801/1‑151 (MQ=255)

                                                                     aCTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGa                                                                              <  8:803077/151‑1 (MQ=255)

                                                                     aCTAGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCtgtg                                                                               >  2:536746/1‑150 (MQ=255)

                                                                        ggCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGaaa                                                                            <  1:1565632/150‑1 (MQ=255)

                                                                                gatgatAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGc                                                                   >  5:1327878/1‑151 (MQ=255)

                                                                                  tgatAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCCGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCAt                                                                 >  4:93071/1‑151 (MQ=255)

                                                                                  tgatAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCAt                                                                 >  7:1402271/1‑151 (MQ=255)

                                                                                    atAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAg                                                               <  5:1178026/151‑1 (MQ=255)

                                                                                    atAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAg                                                               <  1:1490026/151‑1 (MQ=255)

                                                                                      aaTCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAgg                                                              <  7:677095/150‑1 (MQ=255)

                                                                                      aaTCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAgg                                                              <  7:430021/150‑1 (MQ=255)

                                                                                      aaTCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAgg                                                              <  8:908589/150‑1 (MQ=255)

                                                                                      aaTCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGt                                                             <  2:1785710/151‑1 (MQ=255)

                                                                                       aTCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTa                                                            <  6:1250903/151‑1 (MQ=255)

                                                                                         cAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTaa                                                           <  3:1621269/150‑1 (MQ=255)

                                                                                         cAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTaa                                                           <  8:646267/150‑1 (MQ=255)

                                                                                          aaTTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAAtt                                                         >  6:1022220/1‑151 (MQ=255)

                                                                                             tACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAac                                                      >  3:1031621/1‑151 (MQ=255)

                                                                                              aCCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAaca                                                     <  5:446773/151‑1 (MQ=255)

                                                                                                 gAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACg                                                   >  8:125211/1‑150 (MQ=255)

                                                                                                   aaaGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACa                                                <  5:1259945/151‑1 (MQ=255)

                                                                                                     aGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTGAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACAt                                               >  6:1467868/1‑150 (MQ=255)

                                                                                                     aGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGGAATTGATCGGCATAGGTAATTAACACGACATc                                              >  6:924744/1‑151 (MQ=255)

                                                                                                     aGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATc                                              <  1:23803/151‑1 (MQ=255)

                                                                                                          tcatcaGATGACCATGGATAAAACGCCAACAAATGAACATGTAAAAAAATGCTTTGCAGCAATTGATGGTAAAACTGATTAAATCTCGGTAATGCTATTAAATCATTGATGTGAAATTGATCTGAAAAGGTAATTAAAAATACATCACtt                                          >  6:134216/1‑150 (MQ=255)

                                                                                                           catcaGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACttt                                         <  3:216927/150‑1 (MQ=255)

                                                                                                            atcaGATGACCATGGATAAATCGGCAACAAATGAACATGTATAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCa                                       <  3:1777663/151‑1 (MQ=255)

                                                                                                            atcaGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTc                                        >  8:175916/1‑150 (MQ=255)

                                                                                                              caGATGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATc                                     >  3:904109/1‑151 (MQ=255)

                                                                                                              caGATGACCATGGATAAAACGGCAACAAATCAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCAt                                      <  6:495767/150‑1 (MQ=255)

                                                                                                                 aTGACCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCa                                  <  4:1060518/151‑1 (MQ=255)

                                                                                                                    aCCATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATATGTAATTAACACTACATCACTTTCATACCAgtg                               >  5:1035084/1‑151 (MQ=255)

                                                                                                                     ccATGGATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGGTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAgtgt                              <  1:172865/151‑1 (MQ=255)

                                                                                                                         ggATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGttttt                          >  5:1809981/1‑151 (MQ=255)

                                                                                                                         ggATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGtttt                           <  6:237905/150‑1 (MQ=255)

                                                                                                                          gATAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGtttttt                         <  1:1252745/151‑1 (MQ=255)

                                                                                                                           aTAAAACGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGtttttt                         >  7:28861/1‑150 (MQ=255)

                                                                                                                             aaaaGGGAAAAAAAGTAACATGAAAAAAATTGGTTTGATGCCATTTTTGG‑AAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGt                     <  1:1153247/151‑1 (MQ=255)

                                                                                                                             aaaaCGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACNGATTAANTNNGGNNNATGNTNNTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTAc                       <  2:597288/150‑1 (MQ=255)

                                                                                                                              aaaCGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTG‑TTTTTACGTc                    <  8:1601122/151‑1 (MQ=255)

                                                                                                                               atcGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTATATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTc                    <  6:1673663/149‑1 (MQ=255)

                                                                                                                               aaCGGCAACAAATGAACATGTGAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTTCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTc                    <  1:932119/151‑1 (MQ=255)

                                                                                                                                aCGGCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCg                   >  3:1167660/1‑151 (MQ=255)

                                                                                                                                  ggCAACAAATGAACATGTGAAAAAATGCTTTGCAGCCATTGTTGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCt                 <  1:1351314/151‑1 (MQ=255)

                                                                                                                                   gCAACAAATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCtg                <  5:739360/151‑1 (MQ=255)

                                                                                                                                    caacaaATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTAATCCCAGTGTTTTTTACGTCGCtgt               >  3:476717/1‑151 (MQ=255)

                                                                                                                                     aacaaATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCtgtg              >  5:235514/1‑151 (MQ=255)

                                                                                                                                      acaaATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTAATCCGAGTGTTTTTTACGTCGCTgtgt             >  2:1358032/1‑151 (MQ=255)

                                                                                                                                       caaATGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTgtgt             >  8:24204/1‑150 (MQ=255)

                                                                                                                                          aTGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGtaatt         <  3:1339618/151‑1 (MQ=255)

                                                                                                                                           tGAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTTTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGtaatt         <  3:31294/150‑1 (MQ=255)

                                                                                                                                            gAACATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGtaatta        >  6:1814762/1‑150 (MQ=255)

                                                                                                                                             aaCATGTAAAAAAATGCTTTGCAGCCATTGATGTTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGtaattaat      <  7:55517/151‑1 (MQ=255)

                                                                                                                                             aaCATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGtaattaat      <  8:121514/151‑1 (MQ=255)

                                                                                                                                             aaCATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGtaattaat      <  4:146427/151‑1 (MQ=255)

                                                                                                                                              aCATGTAAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGTAATTAATc     <  7:1197390/151‑1 (MQ=255)

                                                                                                                                                 tgtaAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGTAATTAATCGtt  >  3:387528/1‑151 (MQ=255)

GCAGACAAAATCAAACATTAAATGACTGCATTCACCGAGTTGCTGAATATCCTGCCACTCCCCCGCTTCACTGGCGACCTGATGATAATCAATTACCGAAAAGCCATCATCAGATGACCATGGATAAAACGGCAACAAATGAACATGTGAAAAAATGCTTTGCAGCCATTGATGGTAAAACTGATTAAATGTGGGTAATGGTTTTAAATCATTGCTGTGAAATTGATCGGCATAGGTAATTAACACGACATCACTTTCATCCCAGTGTTTTTTACGTCGCTGTGTAATTAATCGTT  >  DH1/2509069‑2509364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`