Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,297,355 A→T N28K (AAT→AAA)  appY ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,297,3550AT97.3% 2036.0 / 22.3 554N28K (AAT→AAA) appYtranscriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (8/7);  new base T (318/221);  total (326/228)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 572 total aligned reads displayed.

TCTTATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATACAC  >  DH1/3297207‑3297501
                                                                                                                                                    |                                                                                                                                                  
   tATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAttt                                                                                                                                                  <  4:1527636/148‑1 (MQ=255)
    aTCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATtttttt                                                                                                                                              <  2:1749643/151‑1 (MQ=255)
     tCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAGCAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAtttttttt                                                                                                                                             >  6:1212681/1‑151 (MQ=255)
      cAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAtttttttt                                                                                                                                             <  4:1690490/150‑1 (MQ=255)
      cAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATtttttttt                                                                                                                                            >  2:1420533/1‑151 (MQ=255)
       aaGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTg                                                                                                                                           >  6:83603/1‑151 (MQ=255)
         gagTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGaa                                                                                                                                         <  4:1445327/151‑1 (MQ=255)
           gTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACg                                                                                                                                       >  5:703050/1‑151 (MQ=255)
            tAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACg                                                                                                                                       >  8:746184/1‑150 (MQ=255)
             aaaTTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACg                                                                                                                                       >  3:589824/1‑149 (MQ=255)
                 ttGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATG‑ATTTTTTTTGAACGAGATAAc                                                                                                                                >  3:1024434/1‑151 (MQ=255)
                   gCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAAct                                                                                                                               >  7:1781651/1‑151 (MQ=255)
                     cAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAActct                                                                                                                             <  3:966218/151‑1 (MQ=255)
                       ggCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTcc                                                                                                                           <  4:523489/151‑1 (MQ=255)
                        gCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCt                                                                                                                          >  1:1619136/1‑151 (MQ=255)
                          aGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGt                                                                                                                        >  8:1071242/1‑151 (MQ=255)
                           gCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGtt                                                                                                                       <  3:864078/151‑1 (MQ=255)
                           gCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGtt                                                                                                                       >  2:871130/1‑151 (MQ=255)
                           gCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGtt                                                                                                                       <  1:1773278/151‑1 (MQ=255)
                            gtCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGttt                                                                                                                      <  1:624178/150‑1 (MQ=255)
                            cTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGttt                                                                                                                      >  5:282356/1‑151 (MQ=255)
                              cATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGttt                                                                                                                      <  4:439145/149‑1 (MQ=255)
                              cATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTta                                                                                                                     <  7:13408/150‑1 (MQ=255)
                                  attCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAAtt                                                                                                                <  3:654015/151‑1 (MQ=255)
                                    tCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTa                                                                                                               <  3:1081329/150‑1 (MQ=255)
                                     cACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTaa                                                                                                              <  6:1570430/150‑1 (MQ=255)
                                     cACGGCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATG‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAAt                                                                                                            <  1:1452386/151‑1 (MQ=255)
                                      aCGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAAt                                                                                                            >  5:786127/1‑151 (MQ=255)
                                       cGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATG‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATc                                                                                                           >  8:493139/1‑150 (MQ=255)
                                        gTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATc                                                                                                           >  8:261638/1‑150 (MQ=255)
                                        gTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCa                                                                                                          <  5:445814/151‑1 (MQ=255)
                                         tCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCa                                                                                                          >  1:1017406/1‑150 (MQ=255)
                                          cGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCaa                                                                                                         <  4:1799026/150‑1 (MQ=255)
                                          cGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCaa                                                                                                         >  6:725757/1‑150 (MQ=255)
                                           gATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAAt                                                                                                       >  4:1117950/1‑151 (MQ=255)
                                            aTAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAAt                                                                                                       <  2:1627571/150‑1 (MQ=255)
                                             aaaaaaaCGTTTAACCTGGTTTGATATCTATATCCACAATTCTTACTTTTCTCGGGGATAACGGTAAATCCCCTCCAATTTTGGACCGTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGa                                                                                                     <  5:1047853/150‑1 (MQ=255)
                                               aaaaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCATGTTTATAATTAAATAAAATGAtt                                                                                                   >  2:1165263/1‑151 (MQ=255)
                                                 aaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGCACGAGCTAACTATCCTGTTTATAATTTAATAAAATGATTgc                                                                                                 >  8:156392/1‑150 (MQ=255)
                                                 aaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGa                                                                                                 <  3:337858/151‑1 (MQ=255)
                                                 aaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGCCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTg                                                                                                  >  5:1795143/1‑150 (MQ=255)
                                                  aaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTATCCTGTTTATAATTAAATCAAATGATTGa                                                                                                 >  6:558121/1‑150 (MQ=255)
                                                   aGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGATCGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGAc                                                                                                >  2:1271163/1‑150 (MQ=255)
                                                      aTAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGAc                                                                                                >  2:213668/1‑147 (MQ=255)
                                                         aaCATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGa                                                                                          >  4:746010/1‑150 (MQ=255)
                                                          aCATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGaa                                                                                         >  6:236135/1‑150 (MQ=255)
                                                            aTGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAAc                                                                                      >  6:113585/1‑151 (MQ=255)
                                                             tGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACt                                                                                     >  8:1286204/1‑151 (MQ=255)
                                                              gTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACt                                                                                     >  5:1498295/1‑150 (MQ=255)
                                                               ttttCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTAc                                                                                   >  3:1126409/1‑151 (MQ=255)
                                                                 ttCTTATCTATATCAACAATTCTTAGTTTAGAGGTGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACg                                                                                  <  2:73344/150‑1 (MQ=255)
                                                                  tCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACgg                                                                                 >  3:415878/1‑150 (MQ=255)
                                                                   cTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACgg                                                                                 >  7:1470854/1‑149 (MQ=255)
                                                                    ttATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTAACAGATGAAAAATACGGAGc                                                                              >  5:1594688/1‑151 (MQ=255)
                                                                     tATGTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGa                                                                                <  1:119459/148‑1 (MQ=255)
                                                                      aTCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGTAGCa                                                                             >  1:505104/1‑150 (MQ=255)
                                                                      aTCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCa                                                                             >  7:34658/1‑150 (MQ=255)
                                                                       tCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCaa                                                                            >  3:1818167/1‑150 (MQ=255)
                                                                        cTATATCAACAATTCTTAGTTTAGAGGTGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTATTTGAAAGAGATAACTCTACTTTTTATAATTAAATCAAAAGATTCACAGAAGAAAACTACgcaaaaaa                                                                           >  8:336159/1‑143 (MQ=255)
                                                                          atatCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCaa                                                                            <  7:836626/147‑1 (MQ=255)
                                                                          atatCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACAt                                                                        <  5:573331/151‑1 (MQ=255)
                                                                            atCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATa                                                                       >  4:915293/1‑150 (MQ=255)
                                                                               aacaaTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATcc                                                                   <  7:697281/151‑1 (MQ=255)
                                                                                 caaTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAAGTATCCTGTTTATATTTAAATAAAATGATTGAAAGATGAAAAATACGGAGAAAACATAATCCAt                                                                 >  3:1323328/1‑151 (MQ=255)
                                                                                 caaTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCAt                                                                 >  6:1417007/1‑151 (MQ=255)
                                                                                   aTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCt                                                               >  6:409464/1‑151 (MQ=255)
                                                                                   aTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCt                                                               >  7:1225073/1‑151 (MQ=255)
                                                                                        tAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTg                                                             <  2:609846/148‑1 (MQ=255)
                                                                                        tAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGc                                                            <  7:1002472/149‑1 (MQ=255)
                                                                                        tAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCAc                                                          >  5:66033/1‑151 (MQ=255)
                                                                                             tAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTAt                                                      <  7:452892/150‑1 (MQ=255)
                                                                                              agagGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATc                                                     >  4:1501415/1‑150 (MQ=255)
                                                                                               gagGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCAt                                                   >  3:1587430/1‑151 (MQ=255)
                                                                                                  gggCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATaaa                                                <  7:1710871/151‑1 (MQ=255)
                                                                                                        aCTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTaaa                                          <  8:1412235/151‑1 (MQ=255)
                                                                                                        aCTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTaa                                           >  8:182869/1‑150 (MQ=255)
                                                                                                        aCTGGTAACTACCTTCTAATTTTGTAGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATTATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTaa                                           <  1:713254/150‑1 (MQ=255)
                                                                                                         cTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTaaa                                          >  5:1266093/1‑150 (MQ=255)
                                                                                                             tAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACa                                       >  8:1741015/1‑149 (MQ=255)
                                                                                                              aaCTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAg                                     <  7:949284/150‑1 (MQ=255)
                                                                                                                   ccTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGAt                                >  2:464254/1‑150 (MQ=255)
                                                                                                                    cTTCTAATTTTGTCGCTTACAATAAACAATGAATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGAt                                >  3:281924/1‑149 (MQ=255)
                                                                                                                      tCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTTAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGAtagt                             <  7:246284/150‑1 (MQ=255)
                                                                                                                      tCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGAtagt                             <  7:111717/150‑1 (MQ=255)
                                                                                                                      tCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGAtagt                             >  5:947829/1‑150 (MQ=255)
                                                                                                                         aaTTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGAtagta                            <  3:1244944/148‑1 (MQ=255)
                                                                                                                          aTTTTGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTc                         >  2:651525/1‑150 (MQ=255)
                                                                                                                           ttttGTCGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCa                        >  7:575621/1‑150 (MQ=255)
                                                                                                                                 cGCTTACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATaaca                 <  1:868119/151‑1 (MQ=255)
                                                                                                                                    ttACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAAcc              >  1:1302939/1‑151 (MQ=255)
                                                                                                                                     tACAATAAACAATGATTTTTTTTTGAACGAGGTAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAAcc              >  5:1118575/1‑150 (MQ=255)
                                                                                                                                     tACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAAcc              >  2:1041864/1‑150 (MQ=255)
                                                                                                                                     tACAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAAcc              >  6:509509/1‑150 (MQ=255)
                                                                                                                                       cAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAAt           >  1:91090/1‑151 (MQ=255)
                                                                                                                                       cAATAAACAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAAt           >  1:30130/1‑151 (MQ=255)
                                                                                                                                            aaCAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAAt      >  4:1295303/1‑151 (MQ=255)
                                                                                                                                             aCAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATa     <  6:1620538/151‑1 (MQ=255)
                                                                                                                                              cAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATac    >  3:14982/1‑151 (MQ=255)
                                                                                                                                              cAATGATTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATac    >  1:397831/1‑151 (MQ=255)
                                                                                                                                                 atgaTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATacac  >  8:635841/4‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCTTATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGAATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATACAC  >  DH1/3297207‑3297501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑