Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,913,413 T→G intergenic (+79/‑175) yjjA → / → opgB hypothetical protein/phosphoglycerol transferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,913,4130TG98.3% 2084.4 / 7.5 541intergenic (+79/‑175)yjjA/opgBhypothetical protein/phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base T (2/7);  new base G (230/302);  total (232/309)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 541 total aligned reads displayed.

GCTGGCAGAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTTACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACGTTGGCTA  >  DH1/3913265‑3913559
                                                                                                                                                    |                                                                                                                                                  
gCTGGCAGAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGAc                                                                                                                                                  >  7:1752442/1‑151 (MQ=255)
  tGGCAGAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGAccc                                                                                                                                                >  1:1818135/1‑151 (MQ=255)
      aGAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGt                                                                                                                                            <  1:89218/151‑1 (MQ=255)
      aGAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGt                                                                                                                                            >  1:137223/1‑151 (MQ=255)
        aaaaGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGtt                                                                                                                                           >  3:1206780/1‑150 (MQ=255)
        aaaaGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTa                                                                                                                                          <  1:709080/151‑1 (MQ=255)
         aaaGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTac                                                                                                                                         >  8:136101/1‑151 (MQ=255)
          aaGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTac                                                                                                                                         >  4:576635/1‑150 (MQ=255)
          aaGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCATGGAAGGTTTGACCCCTGTTac                                                                                                                                         >  2:983698/1‑150 (MQ=255)
            ggTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACg                                                                                                                                      >  2:1758815/1‑151 (MQ=255)
             gggAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACgg                                                                                                                                     <  7:307798/149‑1 (MQ=255)
             gTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACgg                                                                                                                                     <  7:604914/151‑1 (MQ=255)
                aaaaCCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGaa                                                                                                                                  <  3:659493/151‑1 (MQ=255)
                aaaaCCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGaa                                                                                                                                  <  5:736770/151‑1 (MQ=255)
                 aaaCCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAg                                                                                                                                 <  6:1817922/151‑1 (MQ=255)
                  aaCCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGt                                                                                                                                <  5:1243818/151‑1 (MQ=255)
                  aaCCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGt                                                                                                                                <  2:441934/151‑1 (MQ=255)
                     cAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGAc                                                                                                                             >  3:397495/1‑151 (MQ=255)
                     cAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGAc                                                                                                                             >  8:510984/1‑151 (MQ=255)
                        aGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTCCCCGGCTGACGCCGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTCTTACACGGGTATTGACCgg                                                                                                                          >  4:266901/1‑150 (MQ=255)
                        aGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGt                                                                                                                          >  6:1454778/1‑151 (MQ=255)
                             gCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAAt                                                                                                                       <  3:1473607/149‑1 (MQ=255)
                             gCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAAt                                                                                                                       <  8:1636386/149‑1 (MQ=255)
                              cGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCa                                                                                                                     >  5:343288/1‑150 (MQ=255)
                                aTCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCaaa                                                                                                                   >  1:1047604/1‑150 (MQ=255)
                                    ggTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCaaaaa                                                                                                                 <  5:13505/148‑1 (MQ=255)
                                    ggTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCaaaa                                                                                                                  <  2:1228647/147‑1 (MQ=255)
                                    ggTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAg                                                                                                               <  3:1373382/150‑1 (MQ=255)
                                     gtgtTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTc                                                                                                             <  1:415533/151‑1 (MQ=255)
                                       gtTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTg                                                                                                           <  2:633997/151‑1 (MQ=255)
                                           aaaCAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGttt                                                                                                        >  8:887720/1‑150 (MQ=255)
                                            aaCAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTtat                                                                                                      <  6:908933/151‑1 (MQ=255)
                                             aCAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTtata                                                                                                     <  3:1764180/151‑1 (MQ=255)
                                             aCAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTtata                                                                                                     <  1:1589418/151‑1 (MQ=255)
                                             aCAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTtata                                                                                                     <  4:786543/151‑1 (MQ=255)
                                              cAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTtata                                                                                                     >  4:1370747/1‑150 (MQ=255)
                                               aGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATaa                                                                                                    >  7:409630/1‑150 (MQ=255)
                                                ggggCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAAc                                                                                                  <  2:272377/151‑1 (MQ=255)
                                                 gggCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACAGGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACt                                                                                                 >  5:1120747/1‑151 (MQ=255)
                                                 gggCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACt                                                                                                 <  7:46116/151‑1 (MQ=255)
                                                 gggCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACt                                                                                                 <  7:1256076/151‑1 (MQ=255)
                                                 gggCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTATAAACTa                                                                                                <  5:92936/151‑1 (MQ=255)
                                                  ggCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCAGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTa                                                                                                <  5:449426/151‑1 (MQ=255)
                                                   gCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTaa                                                                                               <  1:1679978/151‑1 (MQ=255)
                                                   gCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTa                                                                                                >  7:1586710/1‑150 (MQ=255)
                                                    cTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAAt                                                                                              >  8:264591/1‑151 (MQ=255)
                                                      gAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAAtt                                                                                             <  4:1651302/150‑1 (MQ=255)
                                                      gAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTc                                                                                            <  5:211512/151‑1 (MQ=255)
                                                        aCTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCt                                                                                          <  3:264796/151‑1 (MQ=255)
                                                        aCTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCt                                                                                          >  1:1260009/1‑151 (MQ=255)
                                                          ttCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGCCGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGGTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTa                                                                                         >  5:1721676/1‑150 (MQ=255)
                                                          ttCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTa                                                                                         >  1:807586/1‑150 (MQ=255)
                                                          ttCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTATGTTTATAAACTAATTCCTa                                                                                         >  8:1021755/1‑150 (MQ=255)
                                                           tCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTTACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTa                                                                                         <  6:1644165/149‑1 (MQ=255)
                                                            cATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTaac                                                                                       >  1:175671/1‑150 (MQ=255)
                                                               ttCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTaacaac                                                                                    <  1:315357/150‑1 (MQ=255)
                                                                 ccTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACAt                                                                                  <  8:70120/150‑1 (MQ=255)
                                                                     aaCCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCAt                                                                             <  7:1368861/151‑1 (MQ=255)
                                                                      aCCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCAtt                                                                            >  1:1164651/1‑151 (MQ=255)
                                                                        cgcgAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCAtttt                                                                          <  6:564410/151‑1 (MQ=255)
                                                                               cgccgcGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCGTGCCCGATTCCCTGCTGCTGGACGGTATGACACCTGTTACACGGGAAGTGCCCGTTATCAAAAAAGACTCTTTATAAACTAATTCCTAACAAAATCGCATTTTTTTGac                                                                    >  6:1119265/1‑150 (MQ=255)
                                                                                  cgcgTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACt                                                                 >  7:1098693/1‑150 (MQ=255)
                                                                                  cgcgTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACt                                                                 >  6:807659/1‑150 (MQ=255)
                                                                                   gcgTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAg                                                               <  4:1062952/151‑1 (MQ=255)
                                                                                    cgTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTGTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGa                                                              <  5:1283852/151‑1 (MQ=255)
                                                                                     gTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGa                                                              >  7:1811814/1‑150 (MQ=255)
                                                                                      ttttAGCTGTTACCCGGCTGACGCGGCTCTGTCCTGCCCTTTTCCCTGCTCCTGGACCGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCAGTTTTTTGAGACTAGatt                                                            >  2:770025/1‑149 (MQ=255)
                                                                                       tttAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGaac                                                            <  4:1683705/150‑2 (MQ=255)
                                                                                        ttAGCTGTTACCGGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCGTGTTACACGGGTAGTGAGCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAAGTAGATCGCATTTTTTTGACACTAGAAtt                                                           <  8:384876/150‑1 (MQ=255)
                                                                                        ttAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTg                                                          >  5:262037/1‑151 (MQ=255)
                                                                                              gTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCaa                                                    <  6:1553341/151‑1 (MQ=255)
                                                                                              gTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCGCCTGGACGGTGTGACCCCTGTTCCACGGCAAGTGACCCTAATCAAAAAAGTCTGTTTATAAAGTAATTCCTAACAACATAGCATTTTTTTGACACAAGAATTgcagca                                                     >  3:1260455/1‑150 (MQ=255)
                                                                                                  cccGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATg                                                <  1:1319721/151‑1 (MQ=255)
                                                                                                         gACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACg                                          <  6:467964/150‑1 (MQ=255)
                                                                                                          ccgcgGCTCTCTCCTGCCCTTTTCACTGCTCCTGGACGGTTTGACGCCTGTTTCACGGGAAGTGACCGTTATCAAAATAGTCTTTTTATATACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGcc                                        <  6:1364143/150‑1 (MQ=255)
                                                                                                                 tctctcCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACAt                                 >  6:1781636/1‑151 (MQ=255)
                                                                                                                    ctcCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACAtt                                <  3:1589532/149‑1 (MQ=255)
                                                                                                                     tcCTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATtgtg                             >  8:818767/1‑151 (MQ=255)
                                                                                                                      ccTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATtgt                              <  3:844146/149‑1 (MQ=255)
                                                                                                                      ccTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATtgt                              <  3:617283/149‑1 (MQ=255)
                                                                                                                      ccTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGc                            >  7:1457852/1‑151 (MQ=255)
                                                                                                                       cTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCt                           >  3:46175/1‑151 (MQ=255)
                                                                                                                       cTGCCCTTTTCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCt                           >  7:812504/1‑151 (MQ=255)
                                                                                                                              tttCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCa                      <  2:1438887/149‑1 (MQ=255)
                                                                                                                                tCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCaaaa                   <  7:1177471/150‑1 (MQ=255)
                                                                                                                                tCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCaaaa                   <  5:814134/150‑1 (MQ=255)
                                                                                                                                tCCCTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCaaaa                   >  4:11753/1‑150 (MQ=255)
                                                                                                                                 cccTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAg                 <  8:1208896/151‑1 (MQ=255)
                                                                                                                                  ccTGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGc                <  6:202472/151‑1 (MQ=255)
                                                                                                                                   ntGCTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCt               >  4:997015/2‑151 (MQ=255)
                                                                                                                                      cTCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTa             >  7:236953/1‑150 (MQ=255)
                                                                                                                                       tCCTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTaa            >  3:1314660/1‑150 (MQ=255)
                                                                                                                                       gccTGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTaaa           <  8:1122441/150‑1 (MQ=255)
                                                                                                                                        ccTTGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTaa            <  8:1790582/149‑1 (MQ=255)
                                                                                                                                          tGGACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACGt        >  2:1542874/1‑151 (MQ=255)
                                                                                                                                           ggACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACg         <  5:1536001/149‑1 (MQ=255)
                                                                                                                                           ggACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACg         <  4:1553596/149‑1 (MQ=255)
                                                                                                                                            gACGGTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACGTTg      >  7:702159/1‑151 (MQ=255)
                                                                                                                                               ggTTTGACCCCTGTTACACTGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACGTTGGCt   <  3:1026081/151‑1 (MQ=255)
                                                                                                                                               ggTTTGACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACGTTGGCt   <  2:1018192/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCTGGCAGAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCTCTCTCCTGCCCTTTTCCCTGCTCCTGGACGGTTTTACCCCTGTTACACGGGAAGTGACCGTAATCAAAAAAGTCTGTTTATAAACTAATTCCTAACAACATCGCATTTTTTTGACACTAGAATTGCAGCAATATGGCGACGCCATTACATTGTGCTTCTCAAAAAGCTTAAACGTTGGCTA  >  DH1/3913265‑3913559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: