Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,980,009 A→G *86* (TAA→TAG)  yjhX → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,980,0090AG98.3% 1791.2 / 7.4 482*86* (TAA→TAG) yjhXhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base G (228/242);  total (235/247)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 486 total aligned reads displayed.

GCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAAGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGC  >  DH1/3979861‑3980156
                                                                                                                                                    |                                                                                                                                                   
gCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAggg                                                                                                                                                   >  2:369874/1‑151 (MQ=255)
gCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAggg                                                                                                                                                   >  4:54725/1‑151 (MQ=255)
  cgcgAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGgag                                                                                                                                                 <  5:361891/151‑1 (MQ=255)
    cgAAGGGCTGTTGCTGACCGACTGCACGCTCCCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGagag                                                                                                                                               <  3:758106/151‑1 (MQ=255)
       aGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGttt                                                                                                                                            <  3:1397421/151‑1 (MQ=255)
        gggCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTc                                                                                                                                           <  1:1020605/151‑1 (MQ=255)
               tGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGAc                                                                                                                                     >  7:1745851/1‑150 (MQ=255)
                 gtGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAACAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGt                                                                                                                                  <  7:1271278/150‑1 (MQ=255)
                   gACCGACTGCACGCTCGCCTTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGt                                                                                                                                  <  4:1227277/149‑1 (MQ=255)
                   gACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTa                                                                                                                                 <  3:3308/150‑1 (MQ=255)
                             aCGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACcacttcac                                                                                                                      >  3:1539265/1‑151 (MQ=255)
                                 tCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCNGTCAATGGCCAGNCCTATCGNATCAACACCACCGAGCTGAATNANGTNCGCNCNCNGCTCGATAATCGCTAGGNAGANNTTCNATgnan                                                                                                                                    >  1:748365/1‑130 (MQ=255)
                                 tCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACCGTACACCACTTCAGtttt                                                                                                                  >  6:1471948/1‑151 (MQ=255)
                                  ggCCGTCTTCAAAAAAGTGAAAACGAAAAAACTTATGAAGTGCGTCAATGGCCAGCCCTATCGAATGATCACGACCGTGCTTAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACGACTTCAGTTTTc                                                                                                                 <  4:1482340/150‑1 (MQ=255)
                                  cGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACtttt                                                                                                                  <  8:636051/150‑1 (MQ=255)
                                  cGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACttt                                                                                                                   <  8:1095219/149‑1 (MQ=255)
                                  cGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTc                                                                                                                 <  6:1793742/151‑1 (MQ=255)
                                  cGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGATGAATAAAGTTCGCGCACAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACtttt                                                                                                                  >  8:1230434/1‑150 (MQ=255)
                                   gCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCa                                                                                                                <  6:1411705/151‑1 (MQ=255)
                                    ccGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCat                                                                                                               >  2:840750/1‑151 (MQ=255)
                                      gTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATTAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCatat                                                                                                             <  4:949174/151‑1 (MQ=255)
                                      gTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCatat                                                                                                             <  7:884206/151‑1 (MQ=255)
                                      gTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCatat                                                                                                             >  6:508864/1‑151 (MQ=255)
                                        cTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATc                                                                                                            >  4:1714717/1‑150 (MQ=255)
                                             aaaaaCTGAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGAt                                                                                                      <  4:845355/151‑1 (MQ=255)
                                              aaaaCTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGAt                                                                                                      >  6:687821/1‑150 (MQ=255)
                                               aatcTCAAAAACAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATaa                                                                                                    <  2:482615/148‑1 (MQ=255)
                                                 aCTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATaaaa                                                                                                  <  1:1330155/151‑1 (MQ=255)
                                                    cAAAACCAATAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGGTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGt                                                                                                <  3:572182/150‑1 (MQ=255)
                                                    cAAAACCAAAAAACTTATCAAGTCCGTCAATTGCCAGCCCTATCCAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGt                                                                                                <  5:801620/150‑1 (MQ=255)
                                                    cAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAg                                                                                                 <  7:643513/149‑1 (MQ=255)
                                                     aaaaCCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACGCCACTTCACTTTTCATATCACAGATAAAAGTGa                                                                                              <  1:376009/151‑1 (MQ=255)
                                                     aaaaCCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGa                                                                                              <  4:413743/151‑1 (MQ=255)
                                                      aaaCCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGAt                                                                                             >  1:17749/1‑151 (MQ=255)
                                                        aCCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGc                                                                                           <  8:1764173/151‑1 (MQ=255)
                                                         ccAATAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCTCTAAGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGcc                                                                                          <  2:598820/151‑1 (MQ=255)
                                                          cAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGcc                                                                                          >  7:492789/1‑150 (MQ=255)
                                                            aaaaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTACGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGt                                                                                       >  5:493773/1‑151 (MQ=255)
                                                             aaaaCTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTg                                                                                      >  7:1794689/1‑151 (MQ=255)
                                                             aaaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTg                                                                                      <  1:1075014/151‑1 (MQ=255)
                                                             aaaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTg                                                                                      <  8:273326/151‑1 (MQ=255)
                                                             aaaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTg                                                                                      <  7:746832/151‑1 (MQ=255)
                                                             aaaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTg                                                                                      >  6:103487/1‑151 (MQ=255)
                                                              aaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTg                                                                                      >  8:1642557/1‑150 (MQ=255)
                                                              aaaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGa                                                                                     <  2:1468884/151‑1 (MQ=255)
                                                               aaCTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGat                                                                                    >  1:43166/1‑151 (MQ=255)
                                                                  ttATCAAGTCCTTCAATGGCCAGCCCTATCTAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGatat                                                                                  <  8:418825/150‑1 (MQ=255)
                                                                   tATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATcc                                                                                >  7:1365365/1‑151 (MQ=255)
                                                                   tATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATc                                                                                 <  3:1112953/150‑1 (MQ=255)
                                                                   tATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATc                                                                                 <  3:1292302/150‑1 (MQ=255)
                                                                    aTCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATcc                                                                                <  5:1263500/150‑1 (MQ=255)
                                                                     tCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCg                                                                               >  2:692977/1‑150 (MQ=255)
                                                                      cAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCggg                                                                             >  6:1334380/1‑151 (MQ=255)
                                                                       aaGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGa                                                                            <  8:527654/151‑1 (MQ=255)
                                                                       aaGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAAGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCggg                                                                             >  1:839628/1‑150 (MQ=255)
                                                                        aGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACAACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGa                                                                            >  6:1574524/1‑150 (MQ=255)
                                                                         gTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGa                                                                            >  6:952759/1‑149 (MQ=255)
                                                                            cGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCg                                                                       >  4:229999/1‑151 (MQ=255)
                                                                             gTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCg                                                                       >  2:20072/1‑150 (MQ=255)
                                                                                aaTGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAAc                                                                   >  1:570151/1‑151 (MQ=255)
                                                                                   ggCCAGCCCTATCGAATCAACTCCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATGGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAAccc                                                                 <  7:954153/150‑1 (MQ=255)
                                                                                   ggCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCcg                                                                <  7:1512410/151‑1 (MQ=255)
                                                                                   ggCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCcg                                                                <  7:121632/151‑1 (MQ=255)
                                                                                        gCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAAGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCAt                                                           >  4:648234/1‑151 (MQ=255)
                                                                                         cccTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCAtt                                                          >  3:198048/1‑151 (MQ=255)
                                                                                          ccTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCAttt                                                         <  2:1792421/151‑1 (MQ=255)
                                                                                             aTCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTgg                                                       <  1:1605275/150‑1 (MQ=255)
                                                                                             aTCGAATCAACACCACCGAGCTGAATAAAGTTCGAGCTCAGCTCGATAATCGCTAGGGAGAGTTTATATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCAGCGCATTTgg                                                       >  4:345692/1‑150 (MQ=255)
                                                                                               cGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCtt                                                    >  1:1270235/1‑151 (MQ=255)
                                                                                                     aaCACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCa                                               >  7:296160/1‑150 (MQ=255)
                                                                                                       caccacCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAaag                                            <  1:931493/151‑1 (MQ=255)
                                                                                                        accaccGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCaa                                              >  5:242226/1‑148 (MQ=255)
                                                                                                         ccaccGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAaagaa                                          <  3:1700024/151‑1 (MQ=255)
                                                                                                          caccGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAaagaag                                         <  5:1039148/151‑1 (MQ=255)
                                                                                                           accGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGc                                        >  3:1035428/1‑151 (MQ=255)
                                                                                                            ccGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATTCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGcc                                       <  4:1338330/151‑1 (MQ=255)
                                                                                                              gAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCg                                      >  5:1719298/1‑150 (MQ=255)
                                                                                                               aGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGAt                                    >  2:1147966/1‑151 (MQ=255)
                                                                                                                gCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGAtt                                   >  5:1378964/1‑151 (MQ=255)
                                                                                                                   gAATAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTa                                 <  2:1322378/150‑1 (MQ=255)
                                                                                                                     aTAAAGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTg                              >  3:886640/1‑151 (MQ=255)
                                                                                                                        aaGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGcc                           <  8:697108/151‑1 (MQ=255)
                                                                                                                         aGTTCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCct                          <  5:1620633/151‑1 (MQ=255)
                                                                                                                           ttCGCGCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCctct                        >  8:148669/1‑151 (MQ=255)
                                                                                                                             cgcgCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCttt                      <  1:1403458/151‑1 (MQ=255)
                                                                                                                             cgcgCTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCtt                       <  2:913217/150‑1 (MQ=255)
                                                                                                                              gcgcTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTa                     <  5:868151/151‑1 (MQ=255)
                                                                                                                                gcTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACt                   >  4:1753130/1‑151 (MQ=255)
                                                                                                                                gcTCAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGACGATTTAGTGGCCTCTTTACt                   >  3:484097/1‑151 (MQ=255)
                                                                                                                                   cAGCTCGATAATCGCTAGGGATAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGa                <  7:373482/151‑1 (MQ=255)
                                                                                                                                   cAGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACtt                  <  5:1541249/149‑1 (MQ=255)
                                                                                                                                    aGCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAg               <  3:706885/151‑1 (MQ=255)
                                                                                                                                     gCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAgg              >  8:1333004/1‑151 (MQ=255)
                                                                                                                                     gCTCGATAATCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAgg              >  6:1282864/1‑151 (MQ=255)
                                                                                                                                            aaTCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGaaa        <  4:1038587/150‑1 (MQ=255)
                                                                                                                                            aaTCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGaaa        <  7:226967/150‑1 (MQ=255)
                                                                                                                                              tCGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATAACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAgcg     >  3:1201238/1‑151 (MQ=255)
                                                                                                                                               cGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAgcgc    <  8:78685/151‑1 (MQ=255)
                                                                                                                                               cGCTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAgcg     <  7:1443854/150‑1 (MQ=255)
                                                                                                                                                 cTAGGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAgcgcgc  >  6:904424/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAACTCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCTCAGCTCGATAATCGCTAAGGAGAGTTTCTATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATTTGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGC  >  DH1/3979861‑3980156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑