Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,709,744 G→T G4C (GGT→TGT)  yegR → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,709,7440GT96.8% 1720.7 / 35.8 467G4C (GGT→TGT) yegRhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (6/9);  new base T (229/223);  total (235/232)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 470 total aligned reads displayed.

AGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTACA  >  DH1/1709596‑1709891
                                                                                                                                                    |                                                                                                                                                   
   tACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAtgtg                                                                                                                                                  >  5:668690/1‑149 (MQ=255)
    aCCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTgaga                                                                                                                                               <  1:1701533/151‑1 (MQ=255)
     cccTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGAt                                                                                                                                              <  5:596296/151‑1 (MQ=255)
     cccTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGAACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGAt                                                                                                                                              <  1:496397/151‑1 (MQ=255)
      ccTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGAtt                                                                                                                                             >  2:1573206/1‑150 (MQ=255)
           aGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCATATTCAATTATAATAATCATGCCATTATTATATTTGAACACTAGAGAGTGTCGATGGTATTTAATGGTGGAATGAGAGATGaaaa                                                                                                                                         >  2:1711690/1‑150 (MQ=255)
            gggCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGaaaaa                                                                                                                                        >  8:1119202/1‑150 (MQ=255)
            gggCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAg                                                                                                                                       <  4:110336/151‑1 (MQ=255)
              gCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGAt                                                                                                                                     <  7:678808/151‑1 (MQ=255)
               ccTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGAt                                                                                                                                     >  1:741790/1‑150 (MQ=255)
                  cAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAgct                                                                                                                                 <  3:1217920/151‑1 (MQ=255)
                  cAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAgc                                                                                                                                  <  7:1345575/150‑1 (MQ=255)
                   aaTGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAgctg                                                                                                                                >  3:1298308/1‑151 (MQ=255)
                        ccGTTATTCCTACTTATGTAGGAAATGTTTTACATAACATTTATTATAATGCTATTCAATTATAATTATCATGCCATTATTATATTTAAAGACTTGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCtata                                                                                                                           <  4:1334330/151‑1 (MQ=255)
                         cGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTatat                                                                                                                          <  8:1456423/151‑1 (MQ=255)
                               tCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCatta                                                                                                                     <  5:1728581/150‑1 (MQ=255)
                                gcTACTTATGTATGTAATGTTGTACAGAACATTTATTATTATCCTTTTCAATTATAATTATGATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaa                                                                                                                    <  6:888716/149‑1 (MQ=255)
                                ccTACTTATGTAGTAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaa                                                                                                                    <  3:1012059/150‑1 (MQ=255)
                                ccTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaat                                                                                                                   <  7:1005370/151‑1 (MQ=255)
                                ccTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaat                                                                                                                   <  8:291230/151‑1 (MQ=255)
                                ccTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaa                                                                                                                    <  6:669538/150‑1 (MQ=255)
                                   aCTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaatt                                                                                                                  <  8:1538886/149‑1 (MQ=255)
                                      tATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAt                                                                                                             >  2:1068278/1‑151 (MQ=255)
                                       aTGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTTATTAGTAtt                                                                                                            >  7:833312/1‑151 (MQ=255)
                                        tGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAttt                                                                                                           >  2:472874/1‑151 (MQ=255)
                                         gTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAttt                                                                                                           <  7:54651/150‑1 (MQ=255)
                                          tAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGTGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAttttt                                                                                                         >  2:1458367/1‑151 (MQ=255)
                                           aGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTtta                                                                                                        <  4:945746/151‑1 (MQ=255)
                                            ggAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTttat                                                                                                       >  8:759921/1‑151 (MQ=255)
                                             gAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTttatt                                                                                                      >  1:835079/1‑151 (MQ=255)
                                             gAAATGTTGTACAGAAAATTTATTATAATCCTATTGAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGTGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTttatt                                                                                                      <  1:1108157/151‑1 (MQ=255)
                                              aaaTGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTttatta                                                                                                     <  1:1487998/151‑1 (MQ=255)
                                                aTGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTtattatt                                                                                                   >  4:79877/1‑150 (MQ=255)
                                                 tgttgtACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGt                                                                                                  >  5:1067571/1‑151 (MQ=255)
                                                    tgtACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTg                                                                                               >  7:46501/1‑151 (MQ=255)
                                                    tgtACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGATATATCATTAATTAGTATTTTTATTATGTCTg                                                                                               >  8:1212704/1‑151 (MQ=255)
                                                     gtACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTgg                                                                                              >  3:1754718/1‑151 (MQ=255)
                                                      tACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGt                                                                                             >  3:334829/1‑151 (MQ=255)
                                                      tACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGt                                                                                             >  2:1106198/1‑151 (MQ=255)
                                                       aCAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGtt                                                                                            >  3:936406/1‑151 (MQ=255)
                                                       aCAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGtt                                                                                            <  4:464860/151‑1 (MQ=255)
                                                        aaGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtg                                                                                           <  2:1223237/150‑1 (MQ=255)
                                                                 tattatAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgctgtga                                                                                   >  7:947472/1‑149 (MQ=255)
                                                                 tattatAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGAAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgctgtgc                                                                                   >  7:363606/1‑150 (MQ=255)
                                                                  attatAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCAt                                                                                 >  5:251108/1‑151 (MQ=255)
                                                                     ataATCCTATTCAATTATAATAATCATGCCATTATTATATTTACACACTAGAGAGGGTCGTTTGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAat                                                                              <  5:509590/151‑1 (MQ=255)
                                                                     ataATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAat                                                                              >  3:630388/1‑151 (MQ=255)
                                                                          ccTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatgatg                                                                          <  1:1384599/150‑1 (MQ=255)
                                                                          ccTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatgatg                                                                          <  3:716241/150‑1 (MQ=255)
                                                                           cTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatgatg                                                                          >  6:1821003/1‑149 (MQ=255)
                                                                            tATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTTTTATGTCTGGTTGTGCTGTGCATAatgatg                                                                          >  1:1741458/1‑148 (MQ=255)
                                                                            tATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatgatg                                                                          >  5:1173049/1‑148 (MQ=255)
                                                                             aTTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGAc                                                                      >  7:1422860/1‑151 (MQ=255)
                                                                                 aaTTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGt                                                                  >  6:304178/1‑151 (MQ=255)
                                                                                 aaTTATAATAATCATGACATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAg                                                                   <  8:244445/150‑1 (MQ=255)
                                                                                    tataATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCAGGTTGTGCTGTGCATAATGATGAGACAAGTATc                                                               >  5:1547151/1‑151 (MQ=255)
                                                                                     ataataATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATc                                                               <  2:249073/150‑1 (MQ=255)
                                                                                     ataataATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCg                                                              <  1:1023812/151‑1 (MQ=255)
                                                                                      taataaTCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCgg                                                             >  7:1086425/1‑151 (MQ=255)
                                                                                          aatCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTaa                                                          <  4:227543/150‑1 (MQ=255)
                                                                                            tCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAAtt                                                       >  5:1406403/1‑151 (MQ=255)
                                                                                             cATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAAttt                                                      <  1:1260795/151‑1 (MQ=255)
                                                                                              aTGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTg                                                     >  5:986975/1‑151 (MQ=255)
                                                                                              aTGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTg                                                     <  6:1654963/151‑1 (MQ=255)
                                                                                                gCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTgg                                                    <  4:648674/150‑1 (MQ=255)
                                                                                                  cATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCt                                                 >  8:573600/1‑151 (MQ=255)
                                                                                                   attattATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCtt                                                <  4:163233/151‑1 (MQ=255)
                                                                                                   attattATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCtt                                                <  4:1776411/151‑1 (MQ=255)
                                                                                                   attattATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCtt                                                >  5:1256068/1‑151 (MQ=255)
                                                                                                   attattATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCtt                                                >  1:258349/1‑151 (MQ=255)
                                                                                                    ttattaTATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTg                                               >  3:471434/1‑151 (MQ=255)
                                                                                                     tattatATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTg                                               <  3:1091165/150‑1 (MQ=255)
                                                                                                         atatTTTAACACTCGATAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAATGATAGCTGCTATATCTTTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCCTAATGATGAGACCAGTATCGGTAAATTTGGTCTTTCatat                                          <  6:456330/151‑1 (MQ=255)
                                                                                                         atatTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCatat                                          <  2:836298/151‑1 (MQ=255)
                                                                                                         atatTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCatat                                          <  3:1478764/151‑1 (MQ=255)
                                                                                                           atTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCAtata                                         <  6:1209922/150‑1 (MQ=255)
                                                                                                            tttAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATaa                                        >  3:18747/1‑150 (MQ=255)
                                                                                                               aaaCACTAGAGAGTGTCGTTTGTATTTAATGGGTTAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTTCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGt                                    <  4:550607/151‑1 (MQ=255)
                                                                                                               aaaCACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGGTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGt                                    >  7:1819033/1‑151 (MQ=255)
                                                                                                               aaaCACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAg                                     <  8:1754523/150‑1 (MQ=255)
                                                                                                                aaCACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTa                                   <  1:1831159/151‑1 (MQ=255)
                                                                                                                  cacTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAat                                 >  2:76124/1‑151 (MQ=255)
                                                                                                                   acTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGAATAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAat                                 >  2:607187/1‑150 (MQ=255)
                                                                                                                     tAGAGAGTGTCGTTTGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATAtt                              >  5:1286808/1‑151 (MQ=255)
                                                                                                                      agagagTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATAACATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGCTAAATTTGGTCTTGAATATAAAAGTAATAtt                              >  3:162170/1‑150 (MQ=255)
                                                                                                                             gtCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTa                       >  6:1418944/1‑150 (MQ=255)
                                                                                                                               cGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAATTAATATTCAGCGTaaa                     >  2:665087/1‑150 (MQ=255)
                                                                                                                                gTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACt                   >  1:62750/1‑151 (MQ=255)
                                                                                                                                gTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACt                   <  7:1119341/151‑1 (MQ=255)
                                                                                                                                 ttGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCAGTGCATAATGATGAGACAAGTATCTGTAAATTTGGTCTTGCAAATAAAAGTAATATTCAGAGTAAAGTc                  >  4:1666266/1‑151 (MQ=255)
                                                                                                                                   ggTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCg                 >  2:722374/1‑150 (MQ=255)
                                                                                                                                   ggTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCg                 <  6:1056168/150‑1 (MQ=255)
                                                                                                                                   ggTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCg                 >  8:790097/1‑150 (MQ=255)
                                                                                                                                   ggTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGa                <  4:1525684/151‑1 (MQ=255)
                                                                                                                                     tATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGAt               >  7:307290/1‑150 (MQ=255)
                                                                                                                                        ttAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAAcc           >  7:1494671/1‑151 (MQ=255)
                                                                                                                                              ggggAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAAtact     >  4:38887/1‑151 (MQ=255)
                                                                                                                                                 gAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTaca  <  8:1016970/151‑1 (MQ=255)
                                                                                                                                                 gAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTaca  <  7:1587461/151‑1 (MQ=255)
                                                                                                                                                 gAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTaca  <  8:606988/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTACA  >  DH1/1709596‑1709891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑