Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,5970GC24.3% 480.1 / 59.9 309intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (99/103);  new base C (3/62);  total (110/199)
Fisher's exact test for biased strand distribution p-value = 4.61e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

GCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAA  >  DH1/613447‑613745
                                                                                                                                                      |                                                                                                                                                    
gCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGAGAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAATCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAg                                                                                                                                                      <  8:70358/151‑1 (MQ=255)
    gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaac                                                                                                                                                  >  3:1135992/1‑150 (MQ=255)
    gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                                                                                                                                                  <  6:1693004/151‑1 (MQ=255)
    gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGATTATAGGTCAGTTTCACCTGATGTACGTAAAAAACAGCTTCGGAGCGTTTTTGCTTTTGGAGGGGCAGaaat                                                                                                                                                  >  1:1981177/1‑150 (MQ=37)
    gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAAAACACCCCTTATCGATCACTTTCACAAAATATACGTAAAAACAAACTTAGGCGCGTTATTGCTAATGGAGATGCACaaa                                                                                                                                                   >  8:210209/1‑150 (MQ=255)
      aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                                                                                                                                <  1:2124987/151‑1 (MQ=255)
      aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGATTATCGGTCCGTTTCACCTGATTTACGTAAAAACCCCCTTCCGCGCGGTATTGCTTTTGGAGGGGCAGaa                                                                                                                                                    >  3:2247788/1‑147 (MQ=38)
       cTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCTGCGCGCGTTTCATTTTGGAGGGGCAGaa                                                                                                                                                    >  1:1022356/1‑146 (MQ=37)
       cTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCGGAAGTACGAAAAAACGCGCTCCGGTCTGTTTTTCCGTTTGGAGGGGCAGAAAgctt                                                                                                                                               >  1:436782/1‑148 (MQ=37)
        tGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCGCCTGAGTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGTGCAGAAAGatg                                                                                                                                               >  7:1745549/1‑150 (MQ=255)
         gAAGCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa                                                                                                                                             <  4:1866949/151‑1 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                                                                                                                               <  4:1336014/149‑1 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                                                                                                                               <  1:1467723/149‑1 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                                                                                                                               <  8:1457309/149‑1 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                                                                                                                               <  3:712462/149‑1 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAAAGatga                                                                                                                                              >  7:1304593/1‑150 (MQ=255)
          aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACGGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                                                                                                                                            <  7:672872/151‑1 (MQ=255)
           aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                           <  8:1051756/151‑1 (MQ=255)
            ccgggttatgctgctTGAGCCAGTGATCGATTGCTGGCCTAGATCAATCACTCTCCACTACAGAACCCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGatgaatga                                                                                                                                          <  3:918568/142‑1 (MQ=18)
            cccGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGaataaga                                                                                                                                           >  7:139992/1‑144 (MQ=255)
              cGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACt                                                                                                                                        <  4:160823/151‑1 (MQ=255)
               ggttattgtttttGAGGGAGTGACCGTTTGATGGCCTAGATTATAGACTTTACACTAAAGAACGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTg                                                                                                                                       <  4:1583671/140‑1 (MQ=11)
               gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGaatgaatg                                                                                                                                       >  1:1793421/1‑151 (MQ=255)
               gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                                                                                                                                       <  5:345365/151‑1 (MQ=255)
               gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                                                                                                                                       <  5:949590/151‑1 (MQ=255)
                 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTc                                                                                                                                     <  2:383290/151‑1 (MQ=255)
                 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTc                                                                                                                                     <  8:1326940/151‑1 (MQ=255)
                   aGGCTGCTTGAGCCAGTGAGCTATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                   <  1:2065061/151‑1 (MQ=255)
                   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGCGAGGAAGatcacacccgtgccc                                                                                                                                   >  3:2130804/1‑138 (MQ=25)
                    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                  <  2:745103/151‑1 (MQ=255)
                    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                  <  3:1488078/151‑1 (MQ=255)
                    ggCTGCTGGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                  <  4:377910/151‑1 (MQ=255)
                        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGACGGCTAATCGATCACGTTCACGTCAAATACTTAAAAACACTCTACAGGGCGTATGTGGTTTTGGAGGGGATGAAAGATGAAACACTGTCCacgacg                                                                                                                              >  5:2002430/1‑151 (MQ=255)
                         ctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGACGGCTTATAGATCGGTTTAGCCGGAGTTTCCAAAAAAGGCGCTTATGCGGGTGTTTGCTTGGGGAGTGGCAGTTATATTAAAGACTGTcgccgcggg                                                                                                                             >  1:110762/1‑143 (MQ=255)
                         ctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATAGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTGTTTGGTTTTGGAGGGGCAGAATGATGAAGGACTGTCCacgactc                                                                                                                             >  3:1693360/1‑149 (MQ=21)
                                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGCTTTAAGAAAAAAGCAGCTGCCCCGGCTGTTATCGTGTGGAGGGGCAGCAAGATGAATGACTGTCAACGACGCTATAccc                                                                                                                      >  1:781453/1‑151 (MQ=21)
                                  gTGAGCTATTGCTGGCCTAGTTTAATGACTGTCCACGACAGAACCCGGCTTTTCGGTAATTTTCACGTGATTTACTTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGTAGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCa                                                                                                                     <  2:648717/150‑1 (MQ=18)
                                    gAGCGATTGCTGGCCTAGATGAATGACTCTCCACTACACAACCCGGCTTATCGGTCATTTTAACCTCATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATCAATGACTGTCCACGACGCTATACCCaaa                                                                                                                   <  6:306690/150‑1 (MQ=18)
                                     tgtgATTCCGGGCCTTGTTTAATGAGTGTCGACTAATTAACCCGTCTTTTGGTTGATTTTCACCTGATTTACGTAAAAACCGGATTCGGGGGGTTTTTGCTTTTGTTGGGGCATATAGATTAATTAGTGTCCACGACGCTATACCCaaaa                                                                                                                  <  2:1693004/147‑1 (MQ=255)
                                     aGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGAGTATCGGTCAGTTTCAACTGATTTACGTAAAAACCCGCTTCGCCGGGTTTTTGCTTTTGTATGTGCAGAAAGATGAATGACTGTCCacgactcgatcggcagagg                                                                                                                 >  6:2285620/1‑137 (MQ=18)
                                      gctttggCTGGCGTAGTTCAATGCCTCGCCACCACAGACCCCGGTTTAGGGGTAAGTTTCACCTGATTTACTTAAAAACGCGCGGCGGGGGTTTTTTGCTTTTGCTGGGGCACAAAGATCAATCACTGGCCACGACGCTATACCCAaaaga                                                                                                                <  7:2261395/145‑1 (MQ=255)
                                      gctaTTGCTGGCCTAGTTTAATGACTGGCCACGACACAACCCGGGTTTTCGGTCTGTTTCACCTTATTTACGTAAAACCCCGCTTGGGCGTTTTTTTGCTTTTGGCGGGGCACAAAGATGAATGAGTGTCCACGACGCTATACCCAaaaga                                                                                                                <  7:1946415/148‑1 (MQ=255)
                                       cGATTGCGGGCCTAGATGAATGACTGGCCACGACAGAACCCGGGTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGCCTGGCCACGACGCTATACCCAaaagaa                                                                                                               <  3:1528158/151‑1 (MQ=18)
                                               ggCGTAGATGAATGAGTGTCCACGAAAGAACCCGGCTATTCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGGGGTTTTTTGGTTTTGTCGGGGCACAAAGTTCAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                       <  1:597961/151‑1 (MQ=17)
                                               ggCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCCGCTGCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                       <  3:1919850/151‑1 (MQ=255)
                                                 ccTAGATGAATGACTGACCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGCGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGACAACGAAACAAAAACCAaaagaaaaagactta                                                                                                      >  8:541417/1‑142 (MQ=17)
                                                    aGATGAATGCCTGGCCACCACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCGGCTGCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                   <  3:400255/150‑1 (MQ=17)
                                                      atgaatgaCTGCCCACCACAGAACCCGGCTTATGGGTCACTTTCACGTGATTTCCGTAAAACCCGGCTGCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                <  4:1447403/151‑1 (MQ=17)
                                                       ttaatgaCTGTCCACGACATAACCCGTATTTTGGGTATTTTTCACTTTATTTACTTAAAAACCGGGTTGGGGGGTTTTTTGTTTTTGTTGGGGCAAAAATATTAATGATTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                <  8:1229897/148‑1 (MQ=9)
                                                       acagaccgtGTCCCCTACCGACCCCGGCTTGGGGGCAACTTGCACGTGTTTTACTTAACACCGGGCTGCGGCGGGTTTTTTTTTTTGCTGGGGCCCAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                               <  5:1589277/143‑1 (MQ=2)
                                                        gaatgaCTGAGCACGACATAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGTTTTTTGGTTTTGGCGGGGCTGAATTATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                               <  8:271233/150‑1 (MQ=14)
                                                           tgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTAAGAAATAAACAGCTACCGGCGGTACTACCTTTAGTACTGGCAGAAAGATGAAGCCCTGTCCACGACCAAATAAACAAAAGAAAGCGGGTTATCGGTCAGgtt                                                                                           >  3:653077/1‑151 (MQ=255)
                                                           acaCTGGCCACGAGAGATACGGGCTTTTCGGTCTGTTTCACCTCTTGTCCTTAAAACCGGGCTGCGGCGGGTTTTTTTTTTTGCTGGCGCCCAATGATGAATGCCTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                           <  3:1346934/149‑1 (MQ=2)
                                                             acgtcccACTACAGAACCCGGCTTTTCGGGAATTTTCACGTTATTTAGTTAAAAACCCGCTTCGGCGGGTTTTTGTTTTTGTAGGGGCATAATTATGAATTACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                         <  6:828905/146‑1 (MQ=14)
                                                             acggcccACGACAGAACCCGGCTTATCGGTAATTTTCACCTGATTTACGTAAAAACCGGCTGCGGCGGGTTTTTGGTTTTGTCGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                         <  7:1845842/146‑1 (MQ=14)
                                                             aCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAaaagaa                                                                                                               >  1:1283037/1‑129 (MQ=255)
                                                                    atgatataaCCCGGCTTATCTGTCAGTTTCACTTCATTTTCGTAAAAACCCTTTTCGTCGGGTTTTTGCTTTTGTTGGTGGTCAATTTTGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                  <  2:2090670/144‑1 (MQ=14)
                                                                     cGACAGAACCCGGATTATCGGTAATTTTCACCTGATTTACGTAAAAACCGGCTTCGGCGGTTTTTTGTTTTTGTAGGGGCTCAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                                 <  3:448265/151‑1 (MQ=14)
                                                                         aGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGAGCAGAAAGCAGAATTCCTGTCCACGACGCTATACCCAAAAGAACGCGTCTGATCGCTCCGTTTCACGTGtttaaagt                                                                             >  2:1742960/1‑146 (MQ=11)
                                                                          caaCCGGGCTTATGGGGCATTTGCCGCTGATTTACTTCAAACCGCGCTGGGGCGGGTTTTTGCTTTTGCCGGGGCCCACCCATGCATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                            <  1:1387417/150‑1 (MQ=2)
                                                                           aaCCCGGCTTATCGGTCAGTTTCACCTGATTTACATAAAAAAACAGATACGAGGATTTAAACATTTTCATCGCCAGAAATAATAAAGCATGTCCACGAAACAATAAAAAAAAAAAAGCGGCTTATCGGTCAGTGTCACCTGGTGTACggat                                                                           >  2:2206906/1‑148 (MQ=2)
                                                                           aaCCCGGCTTAGCGGCCAGTTGAACCTCATTTCCGAAAACCCCGGCTGCGGGGGGTTTTTGTTTTTGGCGGGGCTCAATTATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                           <  5:494947/151‑1 (MQ=14)
                                                                               cGGTTTATCGGTAAGTTTCACCTGTTTAACGTAAACCCCGGCGGCGGGGGGTTTTTGGTTTTGTGGGGGCCCAACTATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAc                                                                       <  7:1096759/151‑1 (MQ=11)
                                                                                 gatcatcgcgcAGTTTCCCCTGATTTAGGTAAAAACCCGCTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAATGTTGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAccc                                                                     <  2:267962/141‑1 (MQ=11)
                                                                                  cTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGAAAGTAATCGACTCTATTAGCAAAAGAAAGGCTTTGAGAGTTAAGTTAGACGTGGTTTACGTaaaaagaa                                                                     >  4:883554/1‑147 (MQ=2)
                                                                                    tATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTAGCTATTTCCGGGGAACAAAGATGAAAGAAGGTCGACCAACGTCTACCCTACAGAAACCGGCTTTTCGGTCAGATTAAAATGGTGTACGTAATAAGCCggt                                                                  >  1:1644948/1‑149 (MQ=2)
                                                                                     atgggTAAGTTTCACCTCATTTACGTAAAAACGCGCTTCTGCGGGTTTTTGTTTTTGTAGGCGCAGAAATATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt                                                                 <  6:1400053/148‑1 (MQ=11)
                                                                                       cggcaattttCCCCTGATTAAGGTATTAACCTCCTTCGGCGGGTTTTTGTTTTTGTTGGTGTTCAATTTTGATTGACTTTACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                               <  2:1063383/144‑1 (MQ=2)
                                                                                       cGGGCAGTTTCACCTGATTTACGTAAAACCCCGCTTCGGCGGGTTTTTGCTTTTGGCGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                               <  5:2185096/151‑1 (MQ=255)
                                                                                        gggcAGTTGCACGTTATTAACGAAAAAACCGGCTGCGGGGTTTTTTTGCTTTTGGAGGGGCACAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                              <  7:1640839/148‑1 (MQ=11)
                                                                                         gTCATTATCACGTGATTTTCTTAAGAACCCAGTTCGGCGGGTTTTTGGTTTTGTAGGGGCACAAACATGAATGCCTTTCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                             <  5:162077/151‑1 (MQ=11)
                                                                                          acattttCAGCTGATTACCGAAAACACCCGCTGCGGGGGGTTTTTGTTTTTGGGGGGGCAAAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcg                                                            <  7:1221108/147‑1 (MQ=11)
                                                                                           aaGTTTCGCCTGATTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCTTTTGCCGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                                           <  5:1219901/150‑1 (MQ=255)
                                                                                            aGTTTCACCTGATTTACGTAAAAACCCGATTCGGCGGGTTTTTGCTTTTTGAGTGCCATAAAGAAGAAACACTGTCCACGACCCACTACACAAATGCAAGCGGCTTATCGGTCAGTATATCATGTTGTACGTTATAAACCGCTTcatcagc                                                          >  6:688525/1‑145 (MQ=255)
                                                                                               ttCACTTGATTTACGTAAAAACCCTCTTCGGCGGGTTTTTGTTTTTGCTGGGGCATAATTATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                                        <  4:673289/150‑1 (MQ=11)
                                                                                               ttCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAacc                                                                     >  8:218868/1‑137 (MQ=255)
                                                                                               ttCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTATCTTTTGGAGGTCCAGAAAGATAAATGACTGTACACGACTCTATAACAAAAAGAAAGCGGAATATATATAAAAATCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                                       >  6:479339/1‑151 (MQ=11)
                                                                                                 cccatGATTTACTTAAACCCCCTCGTCGGCGGGTTTTTGTTTTTGCTGGGGCTCAATTATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                     <  6:2008364/147‑1 (MQ=11)
                                                                                                  aCCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGCCGCTATCCCCAAAAGACCGGGCCGTATCGGGCAGTTTCACCGGGTTTACGTAAAAACCCGCTTCGGCGGGTGTTTg                                                    >  7:1008228/1‑151 (MQ=11)
                                                                                                  aCCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACATATGAAGAGGCAGAAATATGAATTAATTTGCAAGACGATATTACCAAAAGAAAGACGCATATACATAAAAATAAAATGGTTTACGTAAAAACCCGCTTCGGCGGGtttttt                                                    >  2:2001927/1‑150 (MQ=2)
                                                                                                   ccTGATTTGCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAACCTGATTCACAGGACACGTCTGAAGACCCGAAACTAATCCGCTTATCGGTCTGGGTCAGCTGGTTTAAGTAAAAACACGCGGCGGCGGGGTTTTgg                                                   >  1:2270078/1‑150 (MQ=2)
                                                                                                   ccTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGttta                                                      >  4:531193/1‑147 (MQ=11)
                                                                                                    cTGATTTACGTAAAAACCCGCTTCGGCATCTTATTGCTTTAGTAGGGGCATAAAGATGAATGACTGTCCAAGACCATATACCCAAAAGAAAGATTATTATAGGTCAGTTACACCTGGTTTACGTAAAAACCCGCTTccacaacataaaac                                                   >  8:1673203/1‑137 (MQ=2)
                                                                                                     tGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTGGGTTTGCCCTGGTGTCCGTTAAAAACCGCATcgcggcggtgttgctg                                                 >  7:940655/1‑137 (MQ=11)
                                                                                                      gattaccGTAACACCCCGCTGCGCCGGGTTTTTGCTTTTGTCGGGGCAGAAACATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                <  1:416666/145‑1 (MQ=11)
                                                                                                      gattaccGTAAACCCCCGCTGCGGCGGGTTTTGGCTTTTGCCGGCGCCCAACCATGAAGGACTGGCCACGACGCTATACCCAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                <  3:1460774/145‑1 (MQ=11)
                                                                                                       aTTTACGTAAAACCCCTCTTCGGCGGGTTTTTGCTTTTGTAGGTGCATAAAGATGAATTACTGTCCACGACGCTATACCCAAAAGAAAGCTGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                               <  8:1882543/151‑1 (MQ=11)
                                                                                                         taccGTAAACCCCCGCGTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAACGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTg                                              <  7:1146304/147‑1 (MQ=11)
                                                                                                          tccgaaaaaaCCCGCTGCGGCGGGTTTTTGCTTTTGCTGGGGCCCAACCATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTgg                                             <  3:2023291/145‑1 (MQ=11)
                                                                                                          tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGCCGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGGTTTTGCTTTTggg                                            >  7:371478/1‑150 (MQ=25)
                                                                                                          aaCGTAAAACCCCTCTTCGGCGGGTTTTTGCTTTTGTAGGCGCACAAACTTGACGGCCTTCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTgg                                             <  5:858874/149‑1 (MQ=11)
                                                                                                            cGTAAAAACCGGCTGCGGCGGGTTTTTGCTTTTGGAGGGGCCCAAAGATGAAGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                          <  5:647166/151‑1 (MQ=255)
                                                                                                                aacaCCCCCTGCGGCGGGTTTTTGCTTTTGCCGGCGCCCAAACATGAATGCCTGGCCACGACGCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCa                                      <  5:728654/148‑1 (MQ=11)
                                                                                                                  ccccgtctgctgcggGTTTTTGTTTTTGTTGGCGTATAAATTTGATTGACTTGACACGACGCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                    <  4:95904/140‑1 (MQ=11)
                                                                                                                    cccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAGATGGGTTTACGTTAAAAAACGGTTAGGCTGGTTTTTGCTTTTGGTGTGGCAgtat                                  >  8:2032538/1‑148 (MQ=11)
                                                                                                                     ccGCTTCGGCGGGTTTTTGTTTTTGTCGGGGCATAAATATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                                 <  8:1381800/151‑1 (MQ=11)
                                                                                                                       gCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTGTACGTAAAAACCCGCTACGGCGGGTTTGAGCTTTTGGAgtgccatatatac                               >  5:2093257/1‑139 (MQ=11)
                                                                                                                          tccgcggGTTTTTGCTTTTGCCGGGGGACCATCTTGATTGACTTGACAAGACGCTCTACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                               <  2:1363832/145‑1 (MQ=11)
                                                                                                                             gcggGTTTTTGCTTTTGGTGGTGCAGAATCATGAATGCCTGGCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                           <  7:765440/149‑1 (MQ=11)
                                                                                                                                                     aGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTAAGATCTTAAGAGCGAAGAGAAATGAAAGACTGTCATCGCCACTATAcaaaaa  >  2:1829139/1‑145 (MQ=2)
                                                                                                                                                      |                                                                                                                                                    
GCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAA  >  DH1/613447‑613745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑