Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6130TG23.8% 243.8 / 34.8 244intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (69/107);  new base G (2/53);  total (77/167)
Fisher's exact test for biased strand distribution p-value = 6.65e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGG  >  DH1/613463‑613754
                                                                                                                                                         |                                                                                                                                             
  tAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑AG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                             >  1:1682426/1‑151 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCTATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                            <  1:2065061/151‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                             <  5:550487/150‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑AG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAATATcc                                                                                                                                             >  4:488037/1‑150 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGTTTGCTGGCCTAGATGAATGACTGTCGACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑GGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                           <  2:685979/151‑1 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                           <  3:1488078/151‑1 (MQ=255)
    ggCTGCTGGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                           <  4:377910/151‑1 (MQ=255)
        gctTGAGCGAGTGTGCGATTGCTGGCGTAGATGAATGACTGTCCTCGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACG‑GG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg                                                                                                                                       <  6:2202972/151‑1 (MQ=21)
        gctTGAGCCTGTGAGCGATTGCTGGCCTAGATGAATGACTTTCCACGTCAGAACCCTTCTTATCGGTCATTTTCACCTGATTTA‑CGTAAAAAGG‑CT‑CTTCGGCTGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  8:1245861/150‑1 (MQ=21)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGACGGCTAATCGATCACGTTCACGTCAAATA‑CTTAAAAACA‑CT‑CTACAGGGCGTATGTGGTTTTGGAGGGGATGAAAGATGAAACACTGTCCacgacg                                                                                                                                       >  5:2002430/1‑151 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg                                                                                                                                       <  1:1275853/151‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  1:2168930/150‑1 (MQ=255)
         ctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGACGGCTTATAGATCGGTTTAGCCGGAGTTT‑CCAAAAAAGG‑CG‑CTTATGCGGGTGTTTGCTTGGGGAGTGGCAGTTATATTAAAGACTGTcgccgcggg                                                                                                                                      >  1:110762/1‑143 (MQ=255)
         ctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATAGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTGTTTGGTTTTGGAGGGGCAGAATGATGAAGGACTGTCCacgactc                                                                                                                                      >  3:1693360/1‑149 (MQ=21)
          ttGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGGATAAGCGGTCTGTATCACCTGATATA‑CGTAAAAAAC‑GG‑CTTCGCCGGCTTATAGGATTTGGAGGGGGAGAAAGATGAAAGAATGTCAACGACGCt                                                                                                                                     >  3:2005281/1‑151 (MQ=25)
            gAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGCCTTATCGGTCAGTTTCACGTGATTTA‑CGTAAAAACC‑CG‑CGTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCCCGACGCtat                                                                                                                                   >  7:1786326/1‑151 (MQ=255)
             aGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAAAC‑AT‑CTTAAGAGCGTATTTTATTTTAAAGGTGAATAAACATGAATAAATGTCCaagaaccaat                                                                                                                                   >  8:2062307/1‑144 (MQ=255)
              gCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTTATTTA‑CTTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGGCCACGACGCTATAc                                                                                                                                 <  7:1382938/151‑1 (MQ=255)
                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCATTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGTTTTTTGCTTTTGGAGGGGCAGAAAGATCAATCACTGTCCACGACGCTATAccc                                                                                                                               <  3:2165673/151‑1 (MQ=255)
                 aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAATCG‑CG‑ATTAGTAGGGTTTTTGGGTTTGGAGGTGCAGAAAGATGAATGAATGTCGacgcagattgacaa                                                                                                                               >  2:1548426/1‑139 (MQ=21)
                  gTGAGCTATTGCTGGCCTAGTTTAATGACTGTCCACGACAGAACCCGGCTTTTCGGTAATTTTCACGTGATTTA‑CTTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCa                                                                                                                              <  2:648717/150‑1 (MQ=18)
                  gTGAGCGATTGCTGGAGTAGATGAATGACTGTCCACTACAGAACCCTGATTATCGGTCAGTTTCACATGATTTA‑CGTAAAAAGC‑CG‑CTTCGGCGGGTTTTTGATTTTGGAGGAGCAGAAAGATTAATGACTGTCCACGACGCTATACCCaa                                                                                                                             <  2:97263/151‑1 (MQ=255)
                    gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCaaaa                                                                                                                           <  5:337733/151‑1 (MQ=255)
                      gctttggCTGGCGTAGTTCAATGCCTCGCCACCACAGACCCCGGTTTAGGGGTAAGTTTCACCTGATTTA‑CTTAAAAACG‑CG‑CGGCGGGGGTTTTTTGCTTTTGCTGGGGCACAAAGATCAATCACTGGCCACGACGCTATACCCAaaaga                                                                                                                         <  7:2261395/145‑1 (MQ=255)
                       cGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCCATAATCGTTCAGTTTCACATGATTTA‑CGTAAAAAAC‑AC‑CTTCGGAGGGTATTTGATTTTGGAGGGCCAGAAAGATAAATAAATGTCAACGACGCTATACCCAaaagc                                                                                                                         >  2:2227657/1‑149 (MQ=12)
                       cGATTGCGGGCCTAGATGAATGACTGGCCACGACAGAACCCGGGTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGCCTGGCCACGACGCTATACCCAaaagaa                                                                                                                        <  3:1528158/151‑1 (MQ=18)
                       cGATGGCTGGCCTAGATTAATGACTGGCCACTACATAACCCGGCTTATCGTTCAGTTTCACCTCATTTA‑CGTAAAAACC‑CG‑CTTCGGGGGGTTTTTGTTTTTGTAGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaa                                                                                                                        <  4:762053/151‑1 (MQ=17)
                        gATTGCTGGCCTAGATGATTGACTGGCCCCGCCTGAACCCGGCTTATCGGTCAGTTGCACGTGATTTA‑CGTAAAACCC‑GG‑CTTGGGCGGGTTTTTGCTTTTGGCGGGGCAGAAACATGAATGACTGTCCACGACGCTAAACCCAaaagaaa                                                                                                                       <  5:956464/151‑1 (MQ=255)
                         aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGGATTATAGGTCAGTTTCACCTGATTAA‑CGTAAAAAAC‑CG‑CTTAGGAAGGGTGTTCCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAaacgaa                                                                                                                        >  7:1025027/1‑149 (MQ=18)
                         aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaaa                                                                                                                       <  8:1804142/150‑1 (MQ=255)
                           tggtGGCATAGATGAATGAGTGGCCACGACAGAACCCGGGTTATGGGTCAGTTTCACCTGATTTA‑CTTAAAAACC‑CG‑CTTGGGGGGTTTTTTGCTTTTGGAGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGc                                                                                                                     <  1:1441091/147‑1 (MQ=17)
                           tGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAAAAAGCATTA‑CCTAAAAAAA‑CG‑CATAGACGGTTTTATACTTTTGAAGTCGCAGAAATATGAATGACTGTAAACGAAAATATACACAAAAGAAAGCg                                                                                                                    >  6:2288680/1‑151 (MQ=255)
                           ggcgggCCTAGATCAATGCCGGTCCACGACAGAACCCGGCTTTTCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CGTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCg                                                                                                                    <  2:341166/147‑1 (MQ=17)
                               ggCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGTTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGc                                                                                                                  <  3:1977576/149‑1 (MQ=255)
                                 ccTAGATGAATGACTGACCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGCGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGACAACGAAACAAAAACCAaaagaaaaagactta                                                                                                               >  8:541417/1‑142 (MQ=17)
                                    agagcaatgaCTGTCCCCGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAACCC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                            <  2:2120850/145‑1 (MQ=255)
                                    aGATGAATGCCTGGCCACCACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAACCC‑GG‑CTGCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                            <  3:400255/150‑1 (MQ=17)
                                     gATGAATGACTGTCCACGACACAAACCAGCTAATCAGTCAGTATAAAATGAAATA‑AGTAAAAACC‑CC‑CTTCTACCTGTATTTGCTATTCGAATCGAAGAAAAATGAAATCCTGTACAAGACCCTATACCCAgcagaagaccgattttatgg                                                                                                          >  4:111454/1‑137 (MQ=255)
                                      attattcaCTGTCACCCACATAACGCGGCTTAGCGTGCAGTTTCACCTTTTTTA‑CGTAATAACC‑CC‑CTTGGGCGGGTTTTTGGTTTTGGTGGGGCTCAAATATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                          <  7:2085597/143‑1 (MQ=0)
                                      atgaatgaCTGCCCACCACAGAACCCGGCTTATGGGTCACTTTCACGTGATTTC‑CGTAAAACCC‑GG‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                         <  4:1447403/151‑1 (MQ=17)
                                      atcaatgaatgGCCACGACATAACCGGGCTTATCGGTAAGTTTCACCGTATTTA‑CGTAAAAACC‑CC‑TTTGGGCGGGTTTTTGGTTTTGGTGGGGCATAAAGATTAATGAGTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                         <  1:878100/148‑1 (MQ=14)
                                       tgatttaCTGGCCCCGCCAGAACCCGGATTTTCGGTCTGTTTAACCTGATTTT‑GTTAAAAAGG‑CT‑GTTCGGCGGGTTTTTGCTTTTGTTGGGGCACAATTATGAATGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                         <  6:1576657/144‑1 (MQ=9)
                                       acagaccgtGTCCCCTACCGACCCCGGCTTGGGGGCAACTTGCACGTGTTTTA‑CTTAACACCG‑GG‑CTGCGGCGGGTTTTTTTTTTTGCTGGGGCCCAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                                        <  5:1589277/143‑1 (MQ=2)
                                        gaatgaCTGAGCACGACATAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGTTTTTTGGTTTTGGCGGGGCTGAATTATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                                        <  8:271233/150‑1 (MQ=14)
                                          atgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAATGTCCACGACACTATACCCAAAAGAAAGcacctaataagacagt                                                                                                      >  8:241662/1‑135 (MQ=14)
                                           acaCTGGCCACGAGAGATACGGGCTTTTCGGTCTGTTTCACCTCTTGTC‑CTTAAAACCG‑GG‑CTGCGGCGGGTTTTTTTTTTTGCTGGCGCCCAATGATGAATGCCTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                                    <  3:1346934/149‑1 (MQ=2)
                                             cctcgccaccaCACAACCGGGCTTATGGGTCATTTTAACTTCA‑TTA‑CCTTAAAACC‑GGTCTTCGGGGGTTTTTTGTTTTTGGCGGGGTACAAATATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                                  <  8:118517/146‑1 (MQ=2)
                                             acgtcccACTACAGAACCCGGCTTTTCGGGAATTTTCACGTTATTTA‑GTTAAAAACC‑CG‑CTTCGGCGGGTTTTTGTTTTTGTAGGGGCATAATTATGAATTACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                                  <  6:828905/146‑1 (MQ=14)
                                             acggcccACGACAGAACCCGGCTTATCGGTAATTTTCACCTGATTTA‑CGTAAAAACC‑GG‑CTGCGGCGGGTTTTTGGTTTTGTCGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                                  <  7:1845842/146‑1 (MQ=14)
                                                taacactaaattaCCCGGATTATCGGGCATTTTCACATGTTTTT‑AGTCAATTGC‑GG‑TTTCGGCTGTTTTTTGCTTTTGTTGGTGCGCAAACTTTAATGACTCTACACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAc                                                                                                 <  1:546885/137‑1 (MQ=0)
                                                     cGACAGAACCCGGATTATCGGTAATTTTCACCTGATTTA‑CGTAAAAACC‑GG‑CTTCGGCGGTTTTTTGTTTTTGTAGGGGCTCAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                                          <  3:448265/151‑1 (MQ=14)
                                                     aaaaaccaCCCGGTTAATCGGCCATTTGCCGCTG‑TTGA‑CGTAAACACCGGG‑CTGCGGGGGGTTTTTGTTTTTGTGGGGGCCAACATATGAATGCCGGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGtttt                                                                                          <  7:1188494/144‑1 (MQ=2)
                                                      gACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCgg                                                                                                           >  2:1711524/1‑133 (MQ=21)
                                                        cAGAACCCGGCTTATCGGGCCTTTGCACCTGATTTA‑CGAAAAACCC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAGATGAATGCCTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACg                                                                                       <  3:640317/151‑1 (MQ=14)
                                                         aGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGt                                                                                                                                               >  1:1047278/1‑94 (MQ=40)
                                                         aGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGAGCAGAAAGCAGAATTCCTGTCCACGACGCTATACCCAAAAGAACGCGTCTGATCGCTCCGTTTCACGTGtttaaagt                                                                                      >  2:1742960/1‑146 (MQ=11)
                                                         aGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTACTTTTGGAGCGGCAGAAAGAAGAATAACTGAAGACTAACATATACCCAAAAGAAAGCGGCTGATCGCTGAGTTTCaaaaaaaaaacgt                                                                                      >  8:707482/1‑139 (MQ=11)
                                                          caaCCGGGCTTATGGGGCATTTGCCGCTGATTTA‑CTTCAAACCG‑CG‑CTGGGGCGGGTTTTTGCTTTTGCCGGGGCCCACCCATGCATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                                     <  1:1387417/150‑1 (MQ=2)
                                                           aaCCCGGCTTAGCGGCCAGTTGAACCTCATTTC‑CGAAAACCCC‑GG‑CTGCGGGGGGTTTTTGTTTTTGGCGGGGCTCAATTATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                                    <  5:494947/151‑1 (MQ=14)
                                                                   ttctctttCAGTTTCTCCTGATTTA‑CTTAACACCC‑TG‑CTGCGGCGGGTTTTTGTTTTTGCTGGCGCTTAATTATGATTGACTTTCCACGACGCTCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCg                                                                             <  6:753700/143‑1 (MQ=2)
                                                                    tATCGGTCAGTTTCCCGTGATTTA‑CTTAAAACCC‑CG‑CTGCGGCGGGTTTTTGCTTTTGTCGGGGCCCAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                            <  5:724040/150‑1 (MQ=11)
                                                                    tATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTAGCTATTTCCGGGGAACAAAGATGAAAGAAGGTCGACCAACGTCTACCCTACAGAAACCGGCTTTTCGGTCAGATTAAAATGGTGTACGTAATAAGCCggt                                                                           >  1:1644948/1‑149 (MQ=2)
                                                                    tATCGGTCAGTTTCACCTGATTTA‑CGTAAAAAAC‑CG‑ATACGGAGATATTAAGATATTCGAGTAGCAAAAAAATGAATTACTCTCAACAACTCTATAAGCAAAACAAATCATATTAAAAATAAGTTTGACCTGGTGTACGTAATAAACCggt                                                                           >  8:2179911/1‑149 (MQ=255)
                                                                    tATCGGGCAGTTTCACGTGATTTA‑CGTAAACACC‑CG‑CTGCGGCGGGTTTTTGGTTTTGGCGGGGCACAAATATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                            <  1:322121/150‑1 (MQ=11)
                                                                       gggTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAGTTGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                         <  5:745513/149‑1 (MQ=255)
                                                                       gggTCAGTTGCCCGTGATTTA‑CTAAAAAACC‑GG‑CTGCGGCGGGTTTTTGCTTTTGTCGGGGCCAAAATATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGGTTTACGTAAAAACCCGCTTcg                                                                        <  7:1165974/150‑1 (MQ=11)
                                                                       cggcaattttCCCCTGATTAA‑GGTATTAACC‑TC‑CTTCGGCGGGTTTTTGTTTTTGTTGGTGTTCAATTTTGATTGACTTTACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                        <  2:1063383/144‑1 (MQ=2)
                                                                       cGGGCAGTTTCACCTGATTTA‑CGTAAAACCC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGCGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                        <  5:2185096/151‑1 (MQ=255)
                                                                        gggcAGTTGCCCCTGATTTA‑CGTAAACACC‑GG‑GTGCGGCGGGTTTTTGCTTTTGGCGGGGGACAACGATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                                       <  3:700317/148‑1 (MQ=11)
                                                                        gggcAGTTGCACGTTATTAA‑CGAAAAAACC‑GG‑CTGCGGGGTTTTTTTGCTTTTGGAGGGGCACAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                                       <  7:1640839/148‑1 (MQ=11)
                                                                         ggcAGTTTCGCCTGATTTA‑CGTAAACCCC‑CG‑CTGCGGCGGGTTTTTGCTTTTGCAGGGGCCCAAAGATGCAGGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                                      <  7:982427/149‑1 (MQ=11)
                                                                         gTCATTATCACGTGATTTT‑CTTAAGAACC‑CA‑GTTCGGCGGGTTTTTGGTTTTGTAGGGGCACAAACATGAATGCCTTTCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                                      <  5:162077/151‑1 (MQ=11)
                                                                          gaatttgcACCTGATTTA‑CGTAAAACCC‑GC‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCACAACCATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcg                                                                     <  5:935950/144‑1 (MQ=11)
                                                                          acattttCAGCTGATTAC‑CGAAAACACC‑CG‑CTGCGGGGGGTTTTTGTTTTTGGGGGGGCAAAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcg                                                                     <  7:1221108/147‑1 (MQ=11)
                                                                           cAGTTTC‑CCTGGCTTACCGTAAAACCC‑CG‑GTGCCGCCGGTTTTTGTTTTTGGCGGGGCCACAGGTTTATCGACTTTCCTCGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                                                    <  1:210220/151‑1 (MQ=2)
                                                                           aaGTTTCGCCTGATTTA‑CGTAAAAACC‑CG‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                                                    <  5:1219901/150‑1 (MQ=255)
                                                                              tttCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATGTACGTAATAACCCg                                                                             >  8:1909985/1‑139 (MQ=255)
                                                                               ttCACTTGATTTA‑CGTAAAAACC‑CT‑CTTCGGCGGGTTTTTGTTTTTGCTGGGGCATAATTATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                                                 <  4:673289/150‑1 (MQ=11)
                                                                               ttCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTATCTTTTGGAGGTCCAGAAAGATAAATGACTGTACACGACTCTATAACAAAAAGAAAGCGGAATATATATAAAAATCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                                                >  6:479339/1‑151 (MQ=11)
                                                                                tCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGCCGCTATACGCAAGAGACAGCGGCTGATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCggggggt                                                               >  7:1124379/1‑147 (MQ=11)
                                                                                 cccatGATTTA‑CTTAAACCCC‑CT‑CGTCGGCGGGTTTTTGTTTTTGCTGGGGCTCAATTATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                              <  6:2008364/147‑1 (MQ=11)
                                                                                      gATTTC‑CGTAAAACCC‑GG‑CTGCGGCGGTTTTTTGGTTTTGTAGGGGCACAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                         <  1:427476/151‑1 (MQ=11)
                                                                                      gATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATAGATCGGAATAACCTCGTGTACGTAAAAACACTCTTcggctgtttatatctat                                                         >  2:1869818/1‑138 (MQ=11)
                                                                                       gattaccGTAAACCCC‑CG‑CTGCGGCGGGTTTTGGCTTTTGCCGGCGCCCAACCATGAAGGACTGGCCACGACGCTATACCCAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                         <  3:1460774/145‑1 (MQ=11)
                                                                                       aTTTA‑GGTAAAACCC‑GT‑CTTCGGCGGGTTTTTGCTTTTTCAGGTGCAGAATTATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                         <  2:2050634/150‑1 (MQ=11)
                                                                                       aTTAA‑CTTAAAAACC‑CG‑CTGCGGCGGGTTTTTGCTTTTGCTGGGGCATAAAGATGATTGACTCTCCTCGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                        <  4:1617076/151‑1 (MQ=11)
                                                                                         ttA‑CGTAACCACC‑CG‑CTGCGGCGGGTTTTTGGTTTTGGAGGCGCACAACTATGAATCACGGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTg                                                       <  1:738060/150‑1 (MQ=11)
                                                                                          ttagttAACAACG‑CG‑CTTCGGCGGGTTTTTGGTTTTGTTGGGGCTCTATTATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTg                                                       <  6:316645/145‑1 (MQ=11)
                                                                                          tA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGCCGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttgttggtgtgggc                                                     >  1:2009161/1‑143 (MQ=11)
                                                                                          tA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGCCGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGGTTTTGCTTTTggg                                                     >  7:371478/1‑150 (MQ=25)
                                                                                              gaaaaacCC‑CG‑CGTCCGGGGGTTTTTGGTTTGGCCGGCGCAGAACCAGGACGGACGGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGGCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                                  <  7:846894/149‑1 (MQ=11)
                                                                                                aaaaaCC‑CG‑CTTCGGCGGGTTTTTGCTTATCGAGAGGCAGAAAGATGAATGAATGTACACTACGCTATACCAAAAAGAAAGCGGCTTATCGGTAAGTTTCACATGTTTTACGTAAAAACGCGCTGGGGCGGGTTTTTgggggtgggggggg                                                >  2:429239/1‑138 (MQ=11)
                                                                                                 aacccc‑cc‑CTTCGGGGGGTTTTGGCTTTTGGAGGGGGAGAAATATGCATCACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCa                                               <  8:1492794/147‑1 (MQ=11)
                                                                                                     ccccgtctgctgcggGTTTTTGTTTTTGTTGGCGTATAAATTTGATTGACTTGACACGACGCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                             <  4:95904/140‑1 (MQ=11)
                                                                                                      c‑cG‑CTTCGGCGGGTTTTTGTTTTTGTCGGGGCATAAATATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                                          <  8:1381800/151‑1 (MQ=11)
                                                                                                         g‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTGTACGTAAAAACCCGCTACGGCGGGTTTGAGCTTTTGGAgtgccatatatac                                        >  5:2093257/1‑139 (MQ=11)
                                                                                                             tccgcggGTTTTTGCTTTTGCCGGGGGACCATCTTGATTGACTTGACAAGACGCTCTACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                        <  2:1363832/145‑1 (MQ=11)
                                                                                                                   gtgttttGATTTTTGAGGTGCCGAAATATGATGTCCTGGACAAGACTCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                               <  7:542448/148‑1 (MQ=11)
                                                                                                                                        gcaagtttgatggccTGTCCAAAACCCTATCCCCAAAAGAACCCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAACCCCGCTGCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGGCCACGACACTATACCCaaaa          <  5:1141073/137‑1 (MQ=11)
                                                                                                                                        aGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTAAGATCTTAAGAGCGAAGAGAAATGAAAGACTGTCATCGCCACTATAcaaaaa           >  2:1829139/1‑145 (MQ=2)
                                                                                                                                                         |                                                                                                                                             
GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTA‑CGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGG  >  DH1/613463‑613754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑