Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA27.7% 274.6 / 23.8 239intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (112/24);  new base A (51/1);  total (210/29)
Fisher's exact test for biased strand distribution p-value = 3.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613620‑613919
                                                                                                                                                        |                                                                                                                                                       
ccgtgtccgctaaagaacgCGGCTTATGGGGAAGTTGCACGTGCTTT‑CGTTAAAACCGGTCTTCGGCGGGTTTTTGGTTTTGTCGGGGCCC‑AACGATGATTGCCTTGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                                                                                         <  7:612568/140‑1 (MQ=2)
       cccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGATAGCCCGATATCGCTCAGTATCTCCTGtt                                                                                                                                                  >  3:1311768/1‑151 (MQ=11)
            aagaaagCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTGAGCTTTT‑GCTCGGCAGCAAACAGGACTGACGGTCCGCGACAAAATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAACTGTTGTACg                                                                                                                                             >  7:522178/1‑151 (MQ=11)
            aagaaagCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAACCG‑GTGCGGCGGGTTTGTGATTTTTGAGTGCGAG‑AAAGATGATGGGCTGTCCACGCGACTCTACACAACACAAAACGGCGGATCGGTCAGGTTGACCTGTTGTAct                                                                                                                                             >  5:2089881/1‑150 (MQ=2)
                    ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAcc                                                                                                                                     >  7:1456476/1‑151 (MQ=25)
                           cGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAAACCGCTTcg                                                                                                                              >  3:266214/1‑151 (MQ=255)
                           cGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAATAACCCGCTAcg                                                                                                                              >  5:817242/1‑151 (MQ=16)
                            ggTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTGcgg                                                                                                                             >  5:380210/1‑151 (MQ=25)
                            ggTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTcgg                                                                                                                             >  3:1359748/1‑151 (MQ=25)
                              tCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGCTgcggcg                                                                                                                           >  3:2167932/1‑151 (MQ=16)
                              tCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTgcggcg                                                                                                                           >  1:1094169/1‑151 (MQ=25)
                               cAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAATAACCCGGTGcggcgg                                                                                                                          >  3:2204839/1‑151 (MQ=255)
                               cAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATAcggcgg                                                                                                                          >  4:404806/1‑151 (MQ=255)
                                 gTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTACGGCGG‑Gt                                                                                                                       >  1:1958972/1‑151 (MQ=25)
                                  tttCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑ATAGATGAATGACTGTCCACGACACTATAAACAAAAGAAAGCGGCTTATCGGTAAGTATAACCTGTTTTACGTAATAAACCGATTccgacggtt                                                                                                                       >  2:14104/1‑143 (MQ=11)
                                  tttCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAcaacggaagagcggat                                                                                                                       >  2:1869746/1‑136 (MQ=11)
                                   ttCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCACTATCAACAGATTTACGTAAATAAACGCTTAGGAGG‑Gatt                                                                                                                     >  8:1667051/1‑151 (MQ=11)
                                      aCCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGCTTCGGCGG‑Ggttgt                                                                                                                   >  4:1388178/1‑148 (MQ=255)
                                       ccTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGGTGCGGCGG‑Gtttgtgc                                                                                                                 >  3:1467723/1‑147 (MQ=255)
                                        cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGTTGCGGCGG‑Gtttgacat                                                                                                                >  2:465971/1‑146 (MQ=255)
                                        cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGG‑Gtttgaact                                                                                                                >  8:3056/1‑146 (MQ=11)
                                         tGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACCCGCTGCGGCGG‑GTGT‑TTGCtt                                                                                                              >  1:1427969/1‑151 (MQ=255)
                                         gctcttACGTAAACACCCG‑CTTCGGGGGTTTTTTGCTTTTTTATTTTAAT‑ATATATGTATTACTGTGAACTATTCTATACCCAAAAGAATGTTTCTTATCTGTAAGTTTTACCTTTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACtt                                                                                                              <  6:1902621/147‑1 (MQ=2)
                                         gcgcttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGATGGTAAT‑AAAGATGAATCACTGCCCACGACACTATACCCAAAAGAAAGCTTCTTATCGGGCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACtt                                                                                                              <  2:2004073/147‑1 (MQ=11)
                                          ggTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAAAAACCCGCTGCGGCGG‑GTTT‑GTACttt                                                                                                             >  1:359873/1‑151 (MQ=255)
                                          gatttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGCATGACTGTCCACGAGTCAATAACCAGAAGATAGGGGCTTCTCGGGCAGTTTCACCTCTTTTACGTAAAAGCCCGCTTCGGCGG‑Gtgt‑tgggttt                                                                                                             >  1:1598442/3‑145 (MQ=11)
                                           gTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGG‑Gtttaagatttt                                                                                                             >  8:1195478/1‑143 (MQ=11)
                                            tttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTGCTTTTg                                                                                                           >  7:1914893/1‑151 (MQ=14)
                                            tttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGG‑GTTT‑TTACttttt                                                                                                           >  3:2015424/1‑150 (MQ=25)
                                             ttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGATGCGGCGG‑GTTT‑ATTCtttttt                                                                                                          >  2:124125/1‑149 (MQ=11)
                                             ttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTgg                                                                                                          >  3:762231/1‑151 (MQ=35)
                                              tACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGG‑GTTT‑ATCCtttttg                                                                                                          >  7:937315/1‑148 (MQ=11)
                                               aCGCAAAACCCCG‑CTTCGCCGGGTTTTTGTTTTTTTTTCTTGAT‑AATGCTTAATGAGTGTTCTCGACACTATACCAAAATGATAGCGTCTTATAGGTGATTCCGACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGa                                                                                                         <  2:1594505/150‑1 (MQ=2)
                                                cGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTACGGCGG‑GTTT‑GTAATTTTTGAgg                                                                                                       >  8:375854/1‑151 (MQ=255)
                                                 gTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGGTTCGGCGG‑GTTT‑GTTCTTTTTGAgtg                                                                                                      >  6:1384204/1‑149 (MQ=11)
                                                   acccaCCCG‑CTTCGGCGTTTTTTTGCTGTTGGAGCCGGAC‑AACGCGGAATGCCTGGCCCCGAGGCGCTCCGCACACTCCTGCGGCGTTACGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAggg                                                                                                      <  1:238862/145‑1 (MQ=2)
                                                   aaaaaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATACGGCGG‑GTTT‑TTACTTTTTGAgggcg                                                                                                    >  4:1931186/1‑149 (MQ=255)
                                                       aCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATACGGCGG‑GTTT‑ATGCTTTTTGAGTGGCAGa                                                                                                 >  6:70964/1‑150 (MQ=11)
                                                       aCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTACGGCGG‑GTTT‑TTTCTTTTGGAGTGGCAGa                                                                                                 >  8:289064/1‑150 (MQ=255)
                                                         ccG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGGTGCGGCGG‑GTTTGTTATTTTTgagtgccatatat                                                                                               >  1:337016/1‑139 (MQ=11)
                                                              ttCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGGTACGGCGG‑GTTT‑ATACTTTTTGAGTGGGAGTAAGa                                                                                             >  2:1067226/1‑148 (MQ=11)
                                                                 ggcggGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAACTGTTTTACGTAAAAAAACGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAgttagttcaatg                                                                                       >  3:412025/1‑140 (MQ=11)
                                                                  gcggGTTTTTGTTTTTGTTGTTGCCG‑CTAGATCATTGATTTACCACGACTATATACCCATAAGAAAGCCGGTTATCGTTCAGTTTTACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGatgaatga                                                                                      <  8:1006152/151‑1 (MQ=2)
                                                                    gggTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTTCGGCGG‑GTTT‑TTCCTTTTTGAGTGGCATAAAGaataagcact                                                                                    >  5:115563/1‑142 (MQ=11)
                                                                        ttttGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATTCGGCGG‑GTTT‑ATACTTTTGGAGTGGCATaaatagcaaagacattcc                                                                                >  8:1642274/1‑136 (MQ=11)
                                                                         tttGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATGCGGCGG‑GTTT‑GAAATTTTTGAGTGGGATAAatatattaccgattccc                                                                               >  7:2082387/1‑138 (MQ=1)
                                                                           tGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGG‑GTTT‑ATAATTTTTGAGTGCCAGAAAGAACAATCACTGTCCACg                                                                             >  8:1651607/1‑151 (MQ=11)
                                                                            gCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAAACCGCTTCGGCGG‑GTTT‑TTCATTTTTGAGTGGGAGTAATatgaaaccctttcgccgg                                                                            >  1:101772/1‑138 (MQ=11)
                                                                            gCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATTCGGCGG‑GTTT‑ATACTTTTTGAGTGCCATAAATataaagcagattccacg                                                                             >  2:607325/1‑138 (MQ=11)
                                                                               tttGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACATGATTTACGTAAAAACCCGATACGGCGG‑GTTT‑TAAATTTTTGAGTGGCAGAAATATGAAAGCCTTTCCGCGacac                                                                         >  8:2206652/1‑151 (MQ=11)
                                                                                 tGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTGCTTTTGGAGTGGCAGAAAGATCAATGACTGGCCACGACACt                                                                        >  7:1667749/1‑150 (MQ=9)
                                                                                      gggCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTTTACGTAAAAAACCGATTCGGCGG‑GTTT‑CAACTTTTGGAGTGGCATATATTTCAATCACTGTCCGCGagaagaacgc                                                                   >  1:1781114/1‑141 (MQ=11)
                                                                                                  tgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGATTCGGCCG‑GTTT‑TTTCTTTTGGAGTGGCAGAAAGATGATTGCCTTTCCCCGAGAATATAACCAAAAGAAAGcc                                                       >  3:1201870/1‑150 (MQ=11)
                                                                                                        cctttattatagacTTTACACTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACTTAAAAACCGGCTTCGGCGG‑GTTT‑TTGCTTTTGGAGGGGCAGAAAGATTAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTAt                                                 <  8:1561773/139‑1 (MQ=11)
                                                                                                         cTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATGCGGCGG‑GTTT‑ATACTTTTTGAGTGGCAGAAATAACTAAGACATTCCACGACACAATACAAAaatgaaaccatcatatc                                                >  3:526459/1‑141 (MQ=2)
                                                                                                          tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGG‑GTTT‑GTACTTTTGGAGTGGGAGAAAGATGAGTCACTGTCCCCGACACGATACCCAAATGAAAGCGACTGATCg                                               >  7:1689237/1‑151 (MQ=11)
                                                                                                           gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATACGGCGG‑GTTT‑TTACTTTTGGAGGGCCATATATAAGAAACACTGTCCACGACACTAGAACAAAAAGAAAACCGATTATCgc                                              >  2:1819875/1‑150 (MQ=11)
                                                                                                            tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTACGGCGG‑GTTT‑ATAATTTTGGAGTGGCATTAAGATCAATCACTGTCCACGACACATGAGCCAAAAGCAAGCGGCATATCgg                                              >  5:407793/1‑150 (MQ=11)
                                                                                                             ccACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTACGGCGG‑GTTT‑AAACTTTTGGAGGGCCAGAAAGAGCTCTGACTTTCGCCGACACGCTAGGCAAAAGAAACCGGCTTATCggac                                            >  5:746658/1‑149 (MQ=11)
                                                                                                               aCGACCCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAATGCAAGCGGCTTATCGGTCat                                          >  8:899239/1‑150 (MQ=255)
                                                                                                                cGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAATATGAATGACTGTCCACGACACTATAAACAAAAGAAAGCGGCTTATCGCTCAGt                                         >  8:92797/1‑151 (MQ=255)
                                                                                                                cGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTAGACCCAAAAGAAAGCGGCTTTTCGGTCAGt                                         >  7:281704/1‑151 (MQ=25)
                                                                                                                  ccccgccccccTAAAGAACCCGGATTATTTGGCAGTTTCACCTGTTTTACGTAATCCGCATTTGCGCCTG‑GTTT‑TTTGTTTTGGTGGGTCCCAAAGTTGAATGACTGTCCAAGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                       <  5:2292968/143‑1 (MQ=2)
                                                                                                                  ccccaaaaCCCAAAACACAGCGGCTTATCGGGCAGTTTAACCTGTTTTACGAAAAACCCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGAACAAAGATGAAGGACTGTCCACTACACTATACCCAAAAGAAAGCGGCtt                                                   <  7:2274899/132‑1 (MQ=11)
                                                                                                                  acacTATACCCAAAAGAAAGCGGCTTATATCTCAGTATCAACTGTTTTACATAAAAACACGCATCCTCTG‑GTTT‑TTACTTTTGGAGGGGCATAAATATAAATAACTGTCCACGACACTATACCCAAAAGAAAGCGGCtt                                                   >  8:2270065/1‑139 (MQ=11)
                                                                                                                   cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGG‑GTTT‑TTACTTTTGGAGTGCGAGATATATGAATCACTTTCCGCGACACTAAAAAAAAAAGAAAGCATCTTATCGGTAAGtaat                                      >  3:1183119/1‑148 (MQ=11)
                                                                                                                   cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGCCAGAAAGAAGAATCACTGTCCACGACACGATACCCAAAAGAAAGCCGCTTATCGGTCAGtttt                                      >  2:508614/1‑151 (MQ=255)
                                                                                                                    acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATCAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGtttt                                      >  2:1896852/1‑150 (MQ=255)
                                                                                                                    acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTTGAGTGGCAGAAAGAAGAATCACTGTCCACGACAATATAAACAAAAGAAAGCAGCTTATCGCTCAGtttt                                      >  8:515008/1‑150 (MQ=11)
                                                                                                                      tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGG‑GTTT‑GTAATTTTGGAGGGGCAGAAAGAGCAAACACTGTCCACGACACTATCGACAAAAGAAAGCGGCTTCGCGGTctcgtat                                      >  6:1151895/1‑142 (MQ=11)
                                                                                                                      tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGATTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGAAAAAACACTGTCCACGACACTATAAACAAAAGAAAGCAACATATCGCTAAGTATTaa                                    >  2:65360/1‑149 (MQ=11)
                                                                                                                       ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGG‑GTTT‑GTACTTTTGGAGGGGCAGATAGAGGAAGGGCTTTCGGCGACACGATCGGAAAATGCATGCGGCTGATCGGAAAGTATTAACt                                  >  6:666733/1‑151 (MQ=2)
                                                                                                                        taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCGCCGACACTATAGCCAAAAGAAAGCTCCTTATCGGTCAttttaaactt                                 >  3:831409/1‑145 (MQ=11)
                                                                                                                        taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGTGGCAGAAAGATGAATGACTGTCCACGACACTATAGCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTg                                 >  1:845769/1‑151 (MQ=25)
                                                                                                                              aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCGCCGACACGATAGCCAAAAGAAAGCGCCATATCGGTCAGTGTTACGTGATGTAc                           >  4:1593520/1‑151 (MQ=14)
                                                                                                                                agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                         >  4:2133636/1‑151 (MQ=40)
                                                                                                                                agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTAAGt                         >  3:140563/1‑151 (MQ=255)
                                                                                                                                   aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  1:1222591/1‑151 (MQ=255)
                                                                                                                                    cggtgCTTTTCGGTGATTTTCACCTTTTTTACTTAAAAACCCGCTTCGGGGG‑GTTT‑TTTGTTTTGGTGGGGCATAAATATGAATGACTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaata                     <  1:1874378/147‑1 (MQ=14)
                                                                                                                                    agCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATTTGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTTTCCACGACACTATAAGAAAAAGAAAGCGGCTTATCGGTCAGTTTTTAGTGATGTACGtaat                      >  6:2273778/1‑150 (MQ=14)
                                                                                                                                     gggtcTTATCGGGAAGTGCCCCCTTTTTTAAGT‑AAAACCCGTTTCGGGGGTTTTT‑TTACTTTTGTAGGGCAACAAATTTCAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                    <  7:780304/147‑1 (MQ=9)
                                                                                                                                      cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                    >  2:274435/1‑150 (MQ=255)
                                                                                                                                        gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 >  1:1679687/1‑151 (MQ=255)
                                                                                                                                         cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  7:1833713/1‑151 (MQ=255)
                                                                                                                                         cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  4:1805931/1‑151 (MQ=255)
                                                                                                                                         cTTATCGGTCAGTTTCACCTCTTTTACGTAAAAACCCGCTACGGCGG‑GTTT‑TTACTATTGGAGGGGCAGAAAGATCAATGACTGTCCACGACACAATACCCAAAAGAAAGCGCCATATCGGTCAGTATTACCTGATCTACGTAATAAACCg                >  4:1357298/1‑151 (MQ=14)
                                                                                                                                           tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  2:167842/1‑151 (MQ=255)
                                                                                                                                           tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACAAAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAAGAAATAAACCGt               >  4:253579/1‑150 (MQ=255)
                                                                                                                                            accgctctgTTGCACCTGATTACCGTAAAACCCCGCTGCGGCGG‑GTTT‑TTTTTTTTGGAGGGGCACAAAGATCAATGACGGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               <  3:1843272/141‑1 (MQ=14)
                                                                                                                                            aTCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAGCCAAAAGAAAGCGGCATATCGCTCAGTTTTACCAGATGTAAGTTATAAAACGTTc             >  6:1029559/1‑151 (MQ=14)
                                                                                                                                                  cAGTTTCACCTTTTTTACGTAAAAACCCGCTTGGGGGG‑TTTT‑TTTTTTTTGGAGGGGCAGAAATATTAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       <  4:2199140/151‑1 (MQ=17)
                                                                                                                                                   aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  1:530581/1‑151 (MQ=255)
                                                                                                                                                   aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  7:2141175/1‑151 (MQ=255)
                                                                                                                                                   aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  5:93011/1‑151 (MQ=255)
                                                                                                                                                     tttCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  6:399122/1‑151 (MQ=255)
                                                                                                                                                      ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  7:39017/1‑151 (MQ=255)
                                                                                                                                                       tCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  2:1743752/1‑151 (MQ=255)
                                                                                                                                                       tCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTACACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  3:1657002/1‑151 (MQ=255)
                                                                                                                                                        cACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  6:2156227/1‑150 (MQ=255)
                                                                                                                                                        cACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  4:974148/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                       
CGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAG‑AAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGG‑GTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613620‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑