Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.29.0% 346.9 / 154.1 144intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (91/7);  new base . (40/0);  total (135/9)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-02

Only 100 of 148 total aligned reads displayed.

TCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936157‑936443
                                                                                                                                         |                                                                                                                                                       
                   gcgtgaaaattctttGGTGGCGGGGGCCGGATTTGACCGCACGCCCTTCGGGATATGAGCCCGACGAGCTACCAGTCTGCTCCACCCCCCGTCACCGTACTGC‑TTTTCTTGATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACcg                                                                                                                         <  3:367297/137‑1 (MQ=11)
                                                       aCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑ATTACTTCATCAAATTATAATTCGTTGAGGGGGCAGTATTTGAACAGACGACCTTCGGGTTATGAGCCAGACGACCTACCAgac                                                                                    >  8:135237/1‑149 (MQ=11)
                                                             gacCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCAGCCAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCGACCAGCCTGCTcc                                                                              >  3:718395/1‑151 (MQ=255)
                                                                                 cccgaGCTACCATTCTTCTCCACCCCGCGGCACCGTCCTGA‑TTTCCATCAGGAAATTTAGTTTTGACGCGGGGTCTTGATATCAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑Tgcg                                                         <  7:875354/149‑1 (MQ=9)
                                                                                          ccAGGCTGCTCCACCCCGCGTCACCGTAATGC‑TTTACTTCATCAAAATTTAATTGGTTGAGCGGGCCGTATTTGAACCGACCCCCTTAGGGTTATTAGACCAACTAGATAACAGGATGCTCCACAACTCTTCCGAGTA‑TGGGCACTATACt                                                >  1:1624229/1‑151 (MQ=9)
                                                                                            aGGCTGCTCCACCCCGCGTCACCGTAGTGC‑GTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGCCCTTCGGGTTATGATCCCGCAGAGCTACCAGGCTGCTCCCCCACGCGTCCGTGGA‑TGCGCACTATACTcc                                              >  7:609709/1‑151 (MQ=18)
                                                                                               ctgctCCACCCCGCGTCACCGTAGTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGAAGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGGTACAAGGATGCTCCACCCCGCGTCAGTGTA‑TGAGCACTATACTCCGcg                                           >  4:1209196/1‑150 (MQ=17)
                                                                                                tgctCAACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTc                                          >  1:330441/1‑151 (MQ=255)
                                                                                                   agctccccGCGTCACCGTACTGC‑TTTACATCAAGAAAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTcgcg                                       <  5:1535/147‑1 (MQ=18)
                                                                                                    ccACCCCGCGTCACCGTACTGG‑GTTACTTCAACAAATTTTAATTCGTTGCGCCGGCCGGATTAGAACCGCCGACCTTCGGGTTATGAGACCACCGATCGACCAGCCTGCTCCACCCAGCGTCCGAGGA‑TGCGCACTATACTCCCGTCGCGt                                      >  8:2075360/1‑151 (MQ=17)
                                                                                                    ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGt                                      >  7:1969848/1‑151 (MQ=255)
                                                                                                    ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGt                                      >  3:676767/1‑151 (MQ=255)
                                                                                                     cACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGtt                                     >  1:632172/1‑151 (MQ=255)
                                                                                                      aCCCCGCGTCACCCTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCGGAGGAGATACAAGCATGCTCAACCCCCCGACGGTGGA‑TCCCCCCTATAAACCCCTCGCtttt                                    >  2:291927/1‑151 (MQ=14)
                                                                                                       ccccGCGTCACCGTACTGCTTTTAATTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGCGTTATGAGCCCGACGAGATACCAGGCTGCTCAACCCCGCGTCAGTGGA‑GGCGCACAATACTCCTCTcgcgacat                                   >  6:1533/1‑147 (MQ=11)
                                                                                                        cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCCGACTATTCTGCGCTAGGGTTTTc                                  >  4:1957326/1‑151 (MQ=11)
                                                                                                        cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTA‑TGCCAAGTATAcaacgatgtagaattg                                  >  6:2103849/1‑136 (MQ=11)
                                                                                                        cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTc                                  >  3:902898/1‑151 (MQ=255)
                                                                                                          cgcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCAt                                >  7:1506839/1‑151 (MQ=255)
                                                                                                            cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  4:1289992/1‑151 (MQ=255)
                                                                                                            cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  5:1214962/1‑151 (MQ=255)
                                                                                                            cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  2:609752/1‑151 (MQ=255)
                                                                                                             gTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGA‑TGCGGACTATACTCCGCTGGAGTTTTCAt                                >  1:1498744/1‑148 (MQ=11)
                                                                                                             gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  6:158657/1‑150 (MQ=255)
                                                                                                             gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  6:422435/1‑150 (MQ=255)
                                                                                                             gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  4:1471373/1‑150 (MQ=255)
                                                                                                              tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCCCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  6:1321972/1‑150 (MQ=255)
                                                                                                              tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  1:2021626/1‑150 (MQ=255)
                                                                                                              tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  3:1397541/1‑150 (MQ=255)
                                                                                                              tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  7:364541/1‑150 (MQ=255)
                                                                                                               cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGA‑TTCGCAGAATACTCCTCTTTCTTATTCATGGAAc                           >  1:443799/1‑151 (MQ=11)
                                                                                                               cACCGTACTGC‑TTTTCTTTCACAAATTTTAATTGGTTGCGGGGGCCGGAttt                                                                                                                               <  6:1499760/52‑1 (MQ=14)
                                                                                                               cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                           >  1:1795301/1‑151 (MQ=255)
                                                                                                                 ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  7:1710018/1‑151 (MQ=255)
                                                                                                                  cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  1:1331495/1‑151 (MQ=255)
                                                                                                                  cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑AGCGCAGAATACTCCGATAGCGTATTCATGGAAccgt                        >  6:760261/1‑149 (MQ=11)
                                                                                                                  cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTATTGCG‑ACTATACTACACTCTATATTTGATTCAACCtt                        >  4:728475/1‑151 (MQ=14)
                                                                                                                  cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGA‑AGCGCAATATACTGCGCTAGAGTTTTCATGCAACCtt                        >  8:313765/1‑151 (MQ=11)
                                                                                                                   gTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑GTCGGAGTATACTCCACTCTATTTTTCAttgacgcttt                       >  3:1102183/1‑142 (MQ=11)
                                                                                                                   gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                       >  1:1692366/1‑151 (MQ=255)
                                                                                                                   gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTAATGCAACCttt                       >  7:59323/1‑151 (MQ=255)
                                                                                                                    tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACAATACACCGCTGGAGTTTTCATGCACCCttat                      >  6:2059731/1‑149 (MQ=14)
                                                                                                                    tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGCGGA‑GTCGCAGTATACTCCGCACTAGTTTTccttcagccgtgt                      >  7:2137902/1‑139 (MQ=11)
                                                                                                                    tACTGC‑TTTACTTCTTCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  8:1194397/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTGATGCAACCtttt                      >  6:1052745/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  4:136388/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  4:97115/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  7:1859531/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  1:488492/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  8:1992695/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  5:992405/1‑151 (MQ=255)
                                                                                                                    cacggC‑TGTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGAGTTGAACCGACGACCTTCGTGTTATGAGCCCGACGAGCCACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      <  3:1212353/147‑1 (MQ=255)
                                                                                                                     aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTCTGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCTCTATACTCCGCTCGCGTTTTCATTCAAccgtgtt                     >  3:1770223/1‑146 (MQ=14)
                                                                                                                     aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGA‑TGGCCACTCTACTCCCCTCGAGTTTTCCTGccccctgtat                     >  7:328264/1‑142 (MQ=11)
                                                                                                                     aCTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                     >  5:1824080/1‑151 (MQ=255)
                                                                                                                      ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCAGTATACACCGCTCGAGTTTTCATTCAACCtttgtt                    >  4:135422/6‑151 (MQ=14)
                                                                                                                      ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGA‑AGCCCAAAATACAACACTATATATTTCATGCAACCtgaatt                    >  8:574297/6‑146 (MQ=11)
                                                                                                                      cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  7:1030945/1‑151 (MQ=255)
                                                                                                                       tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTACTCTCGAGTTTTCATTCAACCttaattt                   >  6:2224912/5‑146 (MQ=14)
                                                                                                                       tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTA‑TGCGCAAAATACAGCAATCTAGATTTCATGCACCCgtttttt                   >  8:553307/5‑151 (MQ=11)
                                                                                                                       tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGA‑TTCGCTCAATTCTTCGCTAGCGATTTCATGGAGCCttgtttt                   >  8:1144831/5‑151 (MQ=11)
                                                                                                                       tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCACCCttttttt                   >  4:629481/1‑151 (MQ=255)
                                                                                                                       tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  2:151105/1‑151 (MQ=255)
                                                                                                                           tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                >  5:2220921/1‑151 (MQ=255)
                                                                                                                            ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  3:1527077/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  5:2078673/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  5:1271019/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  2:339669/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  7:1239881/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTATCATGCAACCTTTTTTTTAccc              >  4:387150/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTA‑TTCGCAGTCTACTACGCAAGAGTTTTCATGCAACCTTTTTTTTAaccc             >  6:562583/1‑151 (MQ=14)
                                                                                                                             tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGA‑GTCGCTGTATACACCACTCTCTTTTTCATGCAGCCGGATTTTTAaccc             >  7:850231/1‑151 (MQ=11)
                                                                                                                             tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGA‑TGCGCAATATACTCCACTAAAGTATTCATTCAACCttaattataaca              >  6:2221422/1‑140 (MQ=11)
                                                                                                                              aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  7:1126233/1‑151 (MQ=255)
                                                                                                                              aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  8:1850549/1‑151 (MQ=255)
                                                                                                                              aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  7:2009133/1‑151 (MQ=255)
                                                                                                                              aCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTAccacc            >  1:1982626/1‑148 (MQ=255)
                                                                                                                              aCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCAACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGA‑TGCGCACTATACTCCGATCGCGTTTTCATGCAACCTTTTTTTTAccacc            >  1:99956/1‑148 (MQ=18)
                                                                                                                               cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTGTTCATGCAACCTTTTTTTTACCCCg            >  6:1693745/1‑151 (MQ=255)
                                                                                                                                ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  7:1606119/1‑150 (MQ=255)
                                                                                                                                 tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTACCCCGa           >  1:1337034/1‑150 (MQ=255)
                                                                                                                                 tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  5:248673/1‑151 (MQ=255)
                                                                                                                                 tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  7:27170/1‑151 (MQ=255)
                                                                                                                                 tcatcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCCTGCACCCTTTTTTTTAACCCGATc         >  3:1566865/1‑151 (MQ=18)
                                                                                                                                  catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc         >  1:163551/1‑151 (MQ=255)
                                                                                                                                  catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc         >  8:1458591/1‑151 (MQ=255)
                                                                                                                                  catcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTA‑TTCGCAGAATACAACGATCGATAATTCGTTCAACCTTTTTTTTAACACGATcc        >  3:995725/1‑150 (MQ=11)
                                                                                                                                   atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc         >  8:1353309/1‑150 (MQ=255)
                                                                                                                                   atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc         >  2:1104495/1‑150 (MQ=255)
                                                                                                                                   atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGA‑ATCGCACTATACTACAATCGAGTTTTCCTTCACCCTTGGTTTTAaccacctcac       >  4:2129767/1‑144 (MQ=11)
                                                                                                                                   atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGCGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTA‑ATCGCAATCTACTCCACAATCTTTTTCATTCAACCGTAAATTTAACCCCATCAc       >  4:1474735/1‑151 (MQ=2)
                                                                                                                                     caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTGTTTTTTACCCCGATCACg      >  2:477517/1‑151 (MQ=255)
                                                                                                                                     caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      >  2:1383841/1‑151 (MQ=255)
                                                                                                                                      atcttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      <  8:340830/147‑1 (MQ=255)
                                                                                                                                      aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  2:1680349/1‑151 (MQ=255)
                                                                                                                                      aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  1:1382021/1‑151 (MQ=255)
                                                                                                                                       aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  2:1299025/1‑150 (MQ=255)
                                                                                                                                       aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  6:409552/1‑150 (MQ=255)
                                                                                                                                        aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  1:1590302/1‑151 (MQ=255)
                                                                                                                                         ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  6:1318388/1‑151 (MQ=255)
                                                                                                                                         |                                                                                                                                                       
TCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGA‑TGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936157‑936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑