Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG23.0% 467.1 / 135.5 215intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (124/37);  new base G (47/1);  total (173/42)
Fisher's exact test for biased strand distribution p-value = 4.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

Only 100 of 224 total aligned reads displayed.

TCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936157‑936447
                                                                                                                                             |                                                                                                                                                      
                      tcatcaataaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTg                                                                                                                                                <  6:373226/118‑1 (MQ=255)
                                                                         aTGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTACTGC‑GTTACTTCATCAATGTATATTTGGTTGCGGGGTGGGGATTTGCCCAGACGACATTCGGGTTATTATACCGACGAGCTAAAACGCTGCTCCaaaaagagtc                                                                       >  8:482554/1‑140 (MQ=2)
                                                                                          ccAGGCTGCTCCACCCCGCGTCACCGTAATGC‑TTTACTTCATCAAAATTTAATTGGTTGAGCGGGCCGTATTTGAACCGACCCCCTTAGGGTTATTAGACCAACTAGATAACAGGATGCTCCACAACTCTTCCGAGTATGGGCACTATACt                                                    >  1:1624229/1‑151 (MQ=9)
                                                                                            aGGCTGCTCCACCCCGCGTCACCGGACGGC‑TTTACTTCATCAAAGTTTTATTGGTTGCGGGGGGAGGATTTGAAGCGACGACCTTGGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAACCCTCGTCGGTGGATGCTCACTATACTcc                                                  >  4:695207/1‑151 (MQ=14)
                                                                                                tgctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCCCTATACTCCGCTc                                              >  7:922710/1‑151 (MQ=255)
                                                                                                   agctccccGCGTCACCGTACTGC‑TTTACATCAAGAAAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                           <  5:1535/147‑1 (MQ=18)
                                                                                                    ccACCCCGCGTCACCGTAGTGC‑TTTACTTCATCAAATTTTTTTTGGTTGAGGGGGCCTGATTTGAACCGAAGACCTTCGGGTTATGAGACAGACGAGCTACCAGGCTGCTCCCCAGCTCGTCCGTGGATGCGCACTATACTCCGGTCGGGt                                          >  1:1638112/1‑151 (MQ=17)
                                                                                                    ccACCCCGCGTCACCGTACTGG‑GTTACTTCAACAAATTTTAATTCGTTGCGCCGGCCGGATTAGAACCGCCGACCTTCGGGTTATGAGACCACCGATCGACCAGCCTGCTCCACCCAGCGTCCGAGGATGCGCACTATACTCCCGTCGCGt                                          >  8:2075360/1‑151 (MQ=17)
                                                                                                    ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                          >  3:676767/1‑151 (MQ=255)
                                                                                                    ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                          >  3:724793/1‑151 (MQ=255)
                                                                                                    ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                          >  7:1969848/1‑151 (MQ=255)
                                                                                                        cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  3:902898/1‑151 (MQ=255)
                                                                                                            cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCACTATACTCCGCTGGCGTTTTCAttc                                  >  4:1305020/1‑149 (MQ=14)
                                                                                                            cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  5:1214962/1‑151 (MQ=255)
                                                                                                             gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  6:422435/1‑150 (MQ=255)
                                                                                                              tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCCCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                 >  6:1321972/1‑150 (MQ=255)
                                                                                                              tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                 >  1:2021626/1‑150 (MQ=255)
                                                                                                               cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGACTATACACCCCACGCGTTTTCATGGAAc                               >  7:432122/1‑151 (MQ=11)
                                                                                                                aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  6:1568467/1‑151 (MQ=255)
                                                                                                                 ccGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCCCACTATACTCCGATCTGTTTTTCATGGAAccg                             >  7:2112010/1‑150 (MQ=11)
                                                                                                                 ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGCGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCCTTTTCATGCAACCt                             >  8:1967899/1‑151 (MQ=255)
                                                                                                                  cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  1:1331495/1‑151 (MQ=255)
                                                                                                                  cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGCAGAATACTCCGATAGCGTATTCATGGAAccgt                            >  6:760261/1‑149 (MQ=11)
                                                                                                                   gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  1:1692366/1‑151 (MQ=255)
                                                                                                                   gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTAATGCAACCttt                           >  7:59323/1‑151 (MQ=255)
                                                                                                                    tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACAATACACCGCTGGAGTTTTCATGCACCCttat                          >  6:2059731/1‑149 (MQ=14)
                                                                                                                    tACTGC‑TTTACTTCTTCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  8:1194397/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  5:992405/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  7:1859531/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  4:136388/1‑151 (MQ=255)
                                                                                                                    tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  4:97115/1‑151 (MQ=255)
                                                                                                                     aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTCTGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTCTATACTCCGCTCGCGTTTTCATTCAAccgtgtt                         >  3:1770223/1‑146 (MQ=14)
                                                                                                                     aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGAATATACTCCGCTCGCGTTTTCATTCAACCttttt                         >  1:321051/1‑151 (MQ=14)
                                                                                                                      ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAAGCCCAAAATACAACACTATATATTTCATGCAACCtgaatt                        >  8:574297/6‑146 (MQ=11)
                                                                                                                       tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACTCTCGAGTTTTCATTCAACCttaattt                       >  6:2224912/5‑146 (MQ=14)
                                                                                                                       tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCttttttt                       >  4:629481/1‑151 (MQ=255)
                                                                                                                          ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAAGCGAAATATACTACGATCGATTTTTCATGCAACCTTTTTtata                     >  6:1180298/2‑150 (MQ=14)
                                                                                                                            ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc                   >  3:1527077/1‑151 (MQ=255)
                                                                                                                            ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc                   >  6:1499029/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  5:1271019/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  2:339669/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  7:1239881/1‑151 (MQ=255)
                                                                                                                             tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGTCGCTGTATACACCACTCTCTTTTTCATGCAGCCGGATTTTTAaccc                 >  7:850231/1‑151 (MQ=11)
                                                                                                                              gttagtgtgttcattAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACAAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 <  4:1217666/138‑1 (MQ=255)
                                                                                                                              gttaggttgatcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 <  7:634812/137‑1 (MQ=255)
                                                                                                                              gttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGAGTTTTCATGCAAGCTTTTTTTTAcccc                 >  1:712751/15‑151 (MQ=17)
                                                                                                                              aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 >  7:1126233/1‑151 (MQ=255)
                                                                                                                               cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTGTTCATGCAACCTTTTTTTTACCCCg                >  6:1693745/1‑151 (MQ=255)
                                                                                                                                ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  7:1606119/1‑150 (MQ=255)
                                                                                                                                 tgggtgttctatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcg                                             <  4:377293/109‑1 (MQ=255)
                                                                                                                                 tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              >  7:27170/1‑151 (MQ=255)
                                                                                                                                 tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              >  5:248673/1‑151 (MQ=255)
                                                                                                                                 tcatcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCCTGCACCCTTTTTTTTAACCCGATc             >  3:1566865/1‑151 (MQ=18)
                                                                                                                                 tcatcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATTCTCCGCTCGGGTTTTGATGCCCCCTTGTTTTTaagccgctc             >  6:212693/1‑143 (MQ=14)
                                                                                                                                   ggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCa            <  8:1276949/142‑1 (MQ=255)
                                                                                                                                   atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  8:1353309/1‑150 (MQ=255)
                                                                                                                                   atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  2:1104495/1‑150 (MQ=255)
                                                                                                                                   atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAATCGCACTATACTACAATCGAGTTTTCCTTCACCCTTGGTTTTAaccacctcac           >  4:2129767/1‑144 (MQ=11)
                                                                                                                                   atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGCGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAATCGCAATCTACTCCACAATCTTTTTCATTCAACCGTAAATTTAACCCCATCAc           >  4:1474735/1‑151 (MQ=2)
                                                                                                                                     ttttcattAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           <  2:770928/144‑1 (MQ=255)
                                                                                                                                     tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgc                                                              >  6:556337/8‑99 (MQ=9)
                                                                                                                                     tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCATTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGa               <  6:58943/139‑1 (MQ=255)
                                                                                                                                     tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGa               >  1:1377262/8‑146 (MQ=255)
                                                                                                                                     tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTAGCTtttt                                       >  5:2188327/8‑122 (MQ=14)
                                                                                                                                     tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCCAAATATACAACGAAATCTTATTCATTCAACCTTAATTATAACCCCATCAcc          >  2:1866022/8‑150 (MQ=2)
                                                                                                                                     tcctatctAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCGGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           <  3:1424823/143‑1 (MQ=255)
                                                                                                                                     caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTGTTTTTTACCCCGATCACg          >  2:477517/1‑151 (MQ=255)
                                                                                                                                     caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  2:1383841/1‑151 (MQ=255)
                                                                                                                                     caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  1:457234/1‑151 (MQ=255)
                                                                                                                                      gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcg                                             <  2:630269/109‑1 (MQ=255)
                                                                                                                                      gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTCCGCTcg                                             >  1:631826/7‑115 (MQ=255)
                                                                                                                                      atcttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          <  8:340830/147‑1 (MQ=255)
                                                                                                                                      aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  6:795081/1‑151 (MQ=255)
                                                                                                                                       ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         <  4:315406/145‑1 (MQ=255)
                                                                                                                                       ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCTCTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTAACCCCATCACggc        >  1:867153/6‑150 (MQ=18)
                                                                                                                                       aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  1:143845/1‑150 (MQ=255)
                                                                                                                                       aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  2:1299025/1‑150 (MQ=255)
                                                                                                                                        tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTgg                                                                  <  7:1556093/88‑1 (MQ=11)
                                                                                                                                        tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAAGCCCACTAAACTCCCCAAGAGATTTCATGCAACCGTTTTTATAACCAGATCACCGAt       >  8:354151/5‑151 (MQ=14)
                                                                                                                                        aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  1:1590302/1‑151 (MQ=255)
                                                                                                                                         ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt      >  4:1133371/1‑151 (MQ=255)
                                                                                                                                         caatAATTGGTTGCGGGGGCCGGATTTGAACCGATGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTCCGCTAGCGTATTGATGCACCCTTGCTTTTACCCCgcgccccgctt      >  1:1480444/4‑141 (MQ=17)
                                                                                                                                         caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCt                                               <  5:580575/107‑1 (MQ=255)
                                                                                                                                         atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAATCGAAGTATACTGCACTCTATTATTCATTCAAGCTTATTTATAACACCATCAcacatt      >  8:1665812/2‑146 (MQ=2)
                                                                                                                                          tttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTGTTTTTACCCCGATCACGGAttt     >  2:1669976/1‑151 (MQ=255)
                                                                                                                                          tttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTGCTCAGGCGTTTTCATGCAACGTTTTTTATACCCAGATCACGCAttt     >  1:1021228/1‑151 (MQ=17)
                                                                                                                                          tttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGAAAAATACTCCGATCTATTTTTCATGCACCCTTATTTTTACCCCGCTAACGCAttt     >  8:1461579/1‑151 (MQ=17)
                                                                                                                                          tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTCCCCCGATCACGGAttt     >  7:322165/1‑151 (MQ=255)
                                                                                                                                          tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  3:484023/1‑151 (MQ=255)
                                                                                                                                          actAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     <  1:1870646/149‑1 (MQ=255)
                                                                                                                                          aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGTATGCGGACTATACTACGCTCGCGTTTTCCTGCCCCCTTAGTTTTACCCCGCTCACgccatc     >  7:341458/3‑146 (MQ=17)
                                                                                                                                           ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     <  1:352310/150‑1 (MQ=255)
                                                                                                                                           ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    >  1:2216914/1‑151 (MQ=255)
                                                                                                                                           atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGa               <  2:2127727/139‑1 (MQ=255)
                                                                                                                                           atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    <  4:1877600/150‑1 (MQ=255)
                                                                                                                                            tAATTGGTTGCGGGGGGCGGATTCGATGCGCAGAAGTTGGGGTAATGACGACGAGTAGCTTGCCGGGTGGTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    <  3:1123095/150‑1 (MQ=255)
                                                                                                                                             aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTcc                                                                      <  2:153714/83‑1 (MQ=31)
                                                                                                                                             aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGc  >  5:698923/1‑151 (MQ=255)
                                                                                                                                             aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGGACTATACACCGCTCGCGTTTTCATGCAGCGGTTGTTTTACCCCGATCCCGGATTTggg  >  5:2035562/1‑150 (MQ=18)
                                                                                                                                             aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTTTTTTTAACCACATCAcacatcgga   >  4:1421250/1‑142 (MQ=21)
                                                                                                                                             |                                                                                                                                                      
TCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936157‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑