Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,326,2870GT25.0% 1309.3 / 144.2 617intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand):  ref base G (303/152);  new base T (32/120);  total (339/278)
Fisher's exact test for biased strand distribution p-value = 6.71e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 619 total aligned reads displayed.

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437
                                                                                                                                                      |                                                                                                                                                      
 aTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGa                                                                                                                                                       <  7:1992897/151‑1 (MQ=33)
  tGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGAACCCATTAACATCGACAATTGa                                                                                                                                                       >  2:708911/1‑150 (MQ=11)
  tGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGAAAGAAAAACCCCAACTGTGACTACAATATCATAATGACGCCTTTTACAGCGCCATTTGa                                                                                                                                                       >  1:1003666/1‑150 (MQ=11)
   gcgcTACTCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCGGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaa                                                                                                                                                      <  6:459574/150‑1 (MQ=18)
    cgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaatc                                                                                                                                                    >  1:988697/1‑149 (MQ=18)
    cgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAAc                                                                                                                                                    >  7:907014/1‑151 (MQ=35)
     gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                   <  5:1182909/151‑1 (MQ=35)
     gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                   <  1:193544/151‑1 (MQ=35)
        aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgat                                                                                                                                                <  5:199929/151‑1 (MQ=38)
          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAAAATGAAAAAGACGCCTTTTACAGCGCGATATGAAACtgatg                                                                                                                                               >  6:1991917/1‑150 (MQ=255)
           cTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                   >  3:27607/1‑145 (MQ=35)
             tATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACa                                                                                                                                            <  1:1772813/150‑1 (MQ=255)
             tATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTCTCTACAAAATGAAAATGACTACTTATAAAGCGCCATTTGAAACTGATGACa                                                                                                                                            >  3:2012928/1‑150 (MQ=14)
              aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaaa                                                                                                                                          <  2:1876469/151‑1 (MQ=255)
                 aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGc                                                                                                                                       <  8:1386568/151‑1 (MQ=255)
                 aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATAACAAACg                                                                                                                                        >  6:257557/1‑150 (MQ=255)
                      tACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAAAGCAACAt                                                                                                                                  >  5:864697/1‑151 (MQ=255)
                      tACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAt                                                                                                                                  >  2:1032649/1‑151 (MQ=255)
                       acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAt                                                                                                                                  <  4:390561/150‑1 (MQ=255)
                       acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACCCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAt                                                                                                                                  <  6:1246129/150‑1 (MQ=255)
                       acacGTTCACTGCAATCTATTGGATTTGAAGGGTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATTAACAAAGCCTACTTTGTCTACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACAtt                                                                                                                                 <  5:1476889/151‑1 (MQ=255)
                        cacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTg                                                                                                                                <  7:1608143/151‑1 (MQ=255)
                          cGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTAGAAAATCATGCAAAACGCAACaatcc                                                                                                                               >  5:1675688/1‑146 (MQ=11)
                           gTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCCTACGTTGTCTAGAATATGAAAATGACGGCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCt                                                                                                                             >  8:1639031/1‑151 (MQ=255)
                             tCACTGCAATCTATTGTATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTg                                                                                                                            <  3:1518857/150‑1 (MQ=255)
                              gacttgaaTCTATTGGATTTTAAGGGTTTTGATGCCGTTTCAGTCTTTAACGACTCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGTCGCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGAt                                                                                                                          <  7:398455/145‑1 (MQ=17)
                              cACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATTACAACCTCAACATTGCCTGAt                                                                                                                          >  8:1907204/1‑151 (MQ=255)
                                atGCAAAGTATTGTATTTTAAGGCTTTTCATGCGGTATTAGTCGTTCACGACGCATCCGGCATGTACTAAGCCTTCTTTGTATTCAATATTAAAATGACTCCTTTTAAAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgc                                                                                                                        <  8:1514435/150‑1 (MQ=19)
                                  gCAATCTATTGTATTTGAAGGGTTTTCTTGCCGTATAAGTGGTTAACGAGGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATgc                                                                                                                        <  4:180868/149‑1 (MQ=17)
                                   cAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACACATATTAAAACCCAATTTGAAACTAATGAAAAAAGCAACATTGACTGAAGCGCt                                                                                                                     >  6:600394/1‑151 (MQ=14)
                                    aaTCTATTGTATTTGAAGGGTTTTCATTCCGTATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTa                                                                                                                    <  3:1929175/151‑1 (MQ=255)
                                      tCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACg                                                                                                                  >  2:2103002/1‑151 (MQ=255)
                                       cTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATCCCAACCGCAACATTGCCTGATCCGCtcccc                                                                                                                 >  2:921055/1‑147 (MQ=255)
                                          ttGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCtt                                                                                                               >  8:1223977/1‑150 (MQ=255)
                                           tGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTAAAACCGAAATTTGAAAATTATGACAAAAGAAACATTGCCTGATGAGCTAAGCTTAt                                                                                                             >  4:281441/1‑151 (MQ=17)
                                            ggATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  7:274939/1‑151 (MQ=255)
                                            ggATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  8:2195106/1‑151 (MQ=255)
                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  5:324307/1‑149 (MQ=255)
                                                ttGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCAATATCAAACTGAAGACAAACGCAAAATTCCCTGATGAACTACGCTTATCACGc                                                                                                        >  4:800128/1‑151 (MQ=17)
                                                  gAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                      <  1:665815/151‑1 (MQ=255)
                                                    aGGGTTTTGTTGGCGGATAAGTGTTTAAAGCCGGTTCTGGCATGAATAAAGCCTTCTTTGTATACAATATTAAAATGTCTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTac                                                                                                    <  4:1343085/151‑1 (MQ=17)
                                                      ggTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTacac                                                                                                  >  3:607945/1‑151 (MQ=255)
                                                         tttCATGCCGGATAAGGCGTTCACGCCGGATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGt                                                                                                <  3:103486/150‑1 (MQ=255)
                                                         tttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGtt                                                                                               >  6:2145011/1‑151 (MQ=255)
                                                          ttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGtt                                                                                               <  6:88920/150‑1 (MQ=255)
                                                           tCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTcc                                                                                             >  3:839053/1‑150 (MQ=255)
                                                            cattgcGGATTAGTGGTTCAAGCCGCATCCGGCATGAAGAAAGCCTTCGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCa                                                                                             <  6:1325164/145‑1 (MQ=25)
                                                             aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACt                                                                                           >  7:1387286/1‑151 (MQ=255)
                                                               gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         >  7:120539/1‑151 (MQ=255)
                                                                  ggATCAGGCGTTCCCCCCGCCTCCTGCCCGAACATAGCCTAAGTTGTCAAGTATCCGAATATGAGCCCTTATAAAGCGCCATTTTCAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                      <  5:2119433/151‑1 (MQ=2)
                                                                  ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                      >  1:1496261/1‑151 (MQ=255)
                                                                  ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                      >  3:98596/1‑151 (MQ=255)
                                                                  ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                      >  7:2200500/1‑151 (MQ=255)
                                                                    aTAAGGGGTTCACGCCGCAACCGGCATGAACAAAGCCTACGTTGTATACAATATGAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                    <  4:1685667/151‑1 (MQ=255)
                                                                        ggCGTTCACGCCGCTTACTGCATGAACAAAGCCTACGTTGTCTACAATATGACAATGACGCCTTTTCCAGCGGCATTTGAACCTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTg                                                                                <  1:490624/151‑1 (MQ=25)
                                                                           gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGATACGCTTATAAGGCCTACACGTTCACTGCAATGTATTGGAt                                                                             >  8:1542000/1‑151 (MQ=255)
                                                                              cACGCCGCATCCGGCATGAACAAAGCCTACGGTGTCTACAATATGAAAATGCCGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                          <  1:1137067/151‑1 (MQ=255)
                                                                                 gccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGa                                                                         >  6:1792608/1‑149 (MQ=255)
                                                                                 ccctcATCCGGCATGAACAAAGCCTACTTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAg                                                                       <  8:2077896/147‑1 (MQ=255)
                                                                                  cctcATCCTGCATGAACATATCCTACGTTGTCTACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAgg                                                                      <  2:1773804/148‑1 (MQ=18)
                                                                                    gcttCCTGCATGTACAAAGCCTTCTTTGTATACAATAATAAAATGCCCCTTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGt                                                                    <  4:1997575/148‑1 (MQ=11)
                                                                                    gcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGAGAAATTTGAAACTTATTAAAAACGAAACATTTCATGATGAGATAAGCTAATCATGCATACACGTTCAATTAAATATATTGGATTTGAAGGGt                                                                    >  4:2226775/1‑151 (MQ=18)
                                                                                     cATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtt                                                                   >  8:1742853/1‑151 (MQ=255)
                                                                                      atgcgtcATGAAGAAATCCTAAGTTGTCTACATTATGAAATTGTCTCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGttt                                                                  <  4:663106/145‑1 (MQ=17)
                                                                                        ccGGGATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtttt                                                                 <  7:1705045/150‑1 (MQ=255)
                                                                                         cGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACACACGCAACATTGCCTCATGCGCTACGCTTATCACGCCTACACGTACACTGCAATCTATTGGATTTGAAGGGTTTTc                                                                >  4:1444187/1‑150 (MQ=255)
                                                                                             atgtataaaGCCTTCTTTTTATACAATATTAAAATGTCTCTTTTTACAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGc                                                            <  2:1346912/144‑1 (MQ=11)
                                                                                             aTGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAAACCAACATTGCCTGATTCGCTACGCTTATCAGGCCTAAACGTTCACTGCAATATATTGTATTTGAAGCGTTTTCATGcc                                                           >  5:732394/1‑151 (MQ=37)
                                                                                              tGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCg                                                          >  3:714904/1‑151 (MQ=255)
                                                                                                aaattatacttcGTAGTCTCCTATATTAAAATGACTAGTTTAACTTCGCCATTTGTAACTGATTACTATCGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCgg                                                         <  8:723746/139‑1 (MQ=11)
                                                                                                aaCAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCgg                                                         >  3:1800618/1‑150 (MQ=255)
                                                                                                aaCAAAGCCTACGTTGTATACAATAACAAAATGACTCTTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGa                                                        <  6:1653485/151‑1 (MQ=14)
                                                                                                  cAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGACATTTGAAACTGATGACAAACGCAACATAGCCACATCCGCTACGCTTATCAGGCATACCCGTTCACTGAAATCTATTGGATTTGAAGGGTTTTCATGCCGGAt                                                       >  1:1645251/1‑150 (MQ=18)
                                                                                                   aaaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGACTGATGAGCTAAGAGTATAAGTACTCGACGTTCAAAGCAATATATTGCATATCAAGGGTTTTCATggagttaa                                                      >  6:1466503/1‑143 (MQ=16)
                                                                                                    aaGCCTACGTTGTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                      <  3:2167562/149‑1 (MQ=255)
                                                                                                      gCCTTCGTTGTATACAATATTAAAATGACTCTTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGc                                                  <  8:1333106/151‑1 (MQ=14)
                                                                                                           cGTTGTCTTAATTATGATAATGACGCCTTTTACAGCGCCATTTGTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTc                                              <  5:495651/150‑1 (MQ=255)
                                                                                                             ttGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcg                                           >  1:857786/1‑151 (MQ=255)
                                                                                                              tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                          >  2:1170161/1‑151 (MQ=255)
                                                                                                              tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                          >  7:1476386/1‑151 (MQ=255)
                                                                                                              tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                          >  7:1041761/1‑151 (MQ=255)
                                                                                                              tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCATATCAGGCCTACACGTTCACTGCAATCTATTGGATTTCAAGCGTTTTCATGACCGATTAGGCGTTCAcgc                                          >  2:977978/1‑151 (MQ=17)
                                                                                                               gTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATCACAAACGCAACATTGCCTCAAGCGCTACGCATATCAGGACTACACCTACACTGCAATCTATTGGATTTGAAGGGTTATCATCCCGGATAAGGCGTTCAcgcc                                         >  4:1886037/1‑151 (MQ=17)
                                                                                                                   aaaaTATGAAAATTACTCCTTTTTCATCTCAATTTTAAACTGTTTTAAACTTCAAGATTGTCTTATGATCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                     <  2:1609880/149‑1 (MQ=2)
                                                                                                                   aCAATATGAAAATGACGCCTTTTACAGCCCCATTTCACACGGATCACCACCGCAACTTTGCCTGCTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                     <  7:1872504/151‑1 (MQ=17)
                                                                                                                       tatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                 >  3:1284712/1‑151 (MQ=255)
                                                                                                                              aTGACGCCTTTTACAGCGCCATTTGAAACTGATGAAAAACGCAACATTGCCTGATGCGCTACGCTTATCATTCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGACGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                          >  4:1169706/1‑151 (MQ=255)
                                                                                                                                   gCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGc                     >  6:1008591/1‑151 (MQ=40)
                                                                                                                                     cTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   >  1:312214/1‑151 (MQ=39)
                                                                                                                                      ttttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcgt                   >  2:387849/1‑148 (MQ=38)
                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   <  5:1328094/149‑1 (MQ=38)
                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTa                  <  8:274457/150‑1 (MQ=38)
                                                                                                                                       ttaacttctccATTTTAAACTGTTTACAAACGCAAGATTGTCTGATGATCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   <  2:221599/140‑1 (MQ=11)
                                                                                                                                          aCAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGtt              <  8:382612/151‑1 (MQ=35)
                                                                                                                                           cAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATATGAAGGGTTTTCATGCCGGATAAGGAGTTCACCCCGCATCCGGCATGAACAAAGCCTACGTTg             >  6:685629/1‑151 (MQ=19)
                                                                                                                                                ccATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc        >  3:418026/1‑151 (MQ=33)
                                                                                                                                                  agttGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACaa      <  1:1314503/149‑1 (MQ=17)
                                                                                                                                                  aTTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACaa      <  2:580020/151‑1 (MQ=33)
                                                                                                                                                  aTTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACa       >  5:1823542/1‑150 (MQ=33)
                                                                                                                                                     tGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAata    <  1:2162351/150‑1 (MQ=31)
                                                                                                                                                      |                                                                                                                                                      
GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑