Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC23.9% 849.8 / 88.1 435G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (95/236);  new base C (101/3);  total (196/239)
Fisher's exact test for biased strand distribution p-value = 3.27e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

GCCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447253‑3447552
                                                                                                                                                      |                                                                                                                                                     
  cACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCcccccc                                                                                                                                                     >  7:439670/1‑151 (MQ=255)
    cGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGccccccccc                                                                                                                                                   >  4:1361465/1‑150 (MQ=255)
     gATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGc                                                                                                                                                  >  3:821860/1‑151 (MQ=255)
           tCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTcccc                                                                                                                                            >  3:1131492/1‑151 (MQ=255)
            cATGGCGGCATCTTCCCCATCGGCCTGTAACCATCGCCGTGCAGCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCGGTGTTTCTTTCGGCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTccccc                                                                                                                                           <  6:250792/151‑1 (MQ=255)
            cATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTccccc                                                                                                                                           >  3:1171625/1‑151 (MQ=255)
                gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCGCCCCCacc                                                                                                                                        >  2:2032281/1‑148 (MQ=255)
                    cATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCata                                                                                                                                     >  5:238219/1‑149 (MQ=255)
                        ttCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGCCAATGcccc                                                                                                                               >  4:1840377/1‑151 (MQ=255)
                          ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGcaccac                                                                                                                             <  5:1377387/151‑1 (MQ=255)
                          ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGcaccac                                                                                                                             <  2:954197/151‑1 (MQ=255)
                                cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATcca                                                                                                                        <  1:883651/150‑1 (MQ=255)
                                cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATcca                                                                                                                        <  2:1164552/150‑1 (MQ=255)
                                  gCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagcc                                                                                                                     <  8:210976/151‑1 (MQ=255)
                                      gTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgcca                                                                                                                 <  1:1352749/151‑1 (MQ=255)
                                      gTAACCATTGCCGTGCATCGCCAAAATGTGCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgcca                                                                                                                 <  5:1838245/151‑1 (MQ=255)
                                        aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCAGCATAAGCACCCCCAccacccgcccgc                                                                                                               >  8:420938/1‑151 (MQ=255)
                                        aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgccag                                                                                                                <  5:1065858/150‑1 (MQ=255)
                                        aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCACCATATGCACCACATccagccgccagc                                                                                                               >  2:563370/1‑151 (MQ=255)
                                           cATTGCCGTGCATCGCCAAAATGTTCGCCGCCAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGCTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCACCCCCCCCCTATGCACCACCTaccgccgccccccgg                                                                                                            >  6:37805/1‑145 (MQ=255)
                                           cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGt                                                                                                            <  1:1763503/151‑1 (MQ=255)
                                            aTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTGCCCCAGCATATTCCCCACATacagccgccaggcggc                                                                                                           >  4:1436759/1‑149 (MQ=255)
                                            aTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCACCCCCAGCCTATCCCCCACATCCAGCCGCCACCCGTc                                                                                                           >  5:158626/1‑151 (MQ=255)
                                              tGCCGTGCATCGCCAAAATGGTCGCCGACAATATACTCTTCCGGCTGGGTTTCTTTCGCCGCTGCGGTGATCCCGGCAACCTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTcc                                                                                                          <  4:852294/150‑1 (MQ=255)
                                               gCCGTGCATCGCCAAAATGTTCGCCGACAATATTCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATAGAGGCAACGTGCTGCATATTATTGCGCGCCCGACCCGCCTCCACCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                        <  2:778709/151‑1 (MQ=255)
                                               gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                        <  2:1447612/151‑1 (MQ=255)
                                                ccGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCCCCACATCCAGCCGCCAGCCGACCat                                                                                                        >  3:29850/1‑150 (MQ=255)
                                                    gCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTTCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTcc                                                                                                   <  1:1006237/151‑1 (MQ=255)
                                                    gCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTcc                                                                                                   <  2:1658947/151‑1 (MQ=255)
                                                     cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCa                                                                                                  <  5:979994/151‑1 (MQ=255)
                                                        cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCACCCCCACCATATGCCACACATCCACCCCCCCCCCGGCCAAAttaacccg                                                                                               >  5:652368/1‑145 (MQ=255)
                                                        cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCGGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACg                                                                                                <  8:1250022/150‑1 (MQ=255)
                                                          ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGTGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                             <  3:2083131/151‑1 (MQ=255)
                                                            aaaaTGTTCGCCGACAATATACGCTTCCGGCGGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCt                                                                                           <  7:631602/151‑1 (MQ=255)
                                                             aaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCAAATGCCCCACATCCCGCCGCCAGCCGTCCATATTCCACGGCGCtt                                                                                          >  2:2120239/1‑151 (MQ=255)
                                                               aTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTc                                                                                        <  2:666621/151‑1 (MQ=255)
                                                               aTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTc                                                                                        <  6:1477466/151‑1 (MQ=255)
                                                                 gtgcGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCa                                                                                       <  3:510123/147‑1 (MQ=255)
                                                                      ccGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCCCTTTCAGCCAGt                                                                                 >  3:1542732/1‑151 (MQ=255)
                                                                        gACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTgg                                                                               <  1:348836/151‑1 (MQ=255)
                                                                        gACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTgg                                                                               >  7:1511981/1‑151 (MQ=255)
                                                                         aCAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCCCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGc                                                                              >  1:61106/1‑151 (MQ=255)
                                                                          cAATATACGCTTCAGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCGCCCCCACCATATTCCCCCAATAACACAGCCAGCCGTACAAATTTAACCGCGCGTTAAGCCAGtttcg                                                                             >  8:76124/1‑147 (MQ=255)
                                                                             gctaCGCGTCCGGCGGGGGTGCTTTCGCCGCTTCGGTGATCCCGGCAACGTGGTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCGGATATGCACCACATCCAGCCGCCAGCCGTCGATATTCCACGGCGCTTTCAGCCAGTGGCggcc                                                                          <  7:2118289/149‑3 (MQ=255)
                                                                              ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACTGCGCTTTCAGCCAGTGGCGGAc                                                                          <  8:1114949/150‑1 (MQ=255)
                                                                                 cGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCAAATGCCCCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGGGGCGGACAAt                                                                       >  7:1391453/1‑150 (MQ=255)
                                                                                 cGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCAAATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAAt                                                                       >  6:590686/1‑150 (MQ=255)
                                                                                  gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAc                                                                     <  5:2007339/151‑1 (MQ=255)
                                                                                  gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAc                                                                     <  6:2017008/151‑1 (MQ=255)
                                                                                   cTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACCTCCAGCCGCCAGCCCTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACt                                                                    >  4:1006438/1‑151 (MQ=255)
                                                                                   cTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTGCCCCCGCATATGCACCCCCTCCAGCCGCCAGCCGTCCCTATTCCACGGCGCGTTCAGCCAGTGGCGGACAATACt                                                                    >  2:1933878/1‑151 (MQ=255)
                                                                                     tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACt                                                                    <  4:782068/149‑1 (MQ=255)
                                                                                     tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACt                                                                    <  7:753236/149‑1 (MQ=255)
                                                                                      ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTc                                                                 <  4:335867/151‑1 (MQ=255)
                                                                                         gCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCACCCCCAGCATATGCACCACATACAGCCGCCAGCCCTCCATATTCCACGGCGCTTTCACCCAGTGGCGGACAATACTttcttc                                                              >  1:230152/1‑151 (MQ=255)
                                                                                          cTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCACCCCCCCCATATTCACCACATACAGCCCCCCCCCCGCCATAATACACGGCGCGTTAAGCCACTGGCGGCCAAAAATGTCttgc                                                             >  7:1189689/1‑149 (MQ=255)
                                                                                            gggTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTcccc                                                           <  5:1702242/151‑1 (MQ=255)
                                                                                                ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                        <  7:1694559/150‑1 (MQ=255)
                                                                                                ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                        <  3:1368738/150‑1 (MQ=255)
                                                                                                   tttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCCCCACATCCCGCCGCCAGCCGTCCATATTCCACCGCGCTTTCAGCCAGTGGCGGAAAATACTGTCGTCCCCGCGATa                                                     >  8:198850/1‑150 (MQ=255)
                                                                                                     tcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaaa                                                   <  2:1402746/150‑1 (MQ=255)
                                                                                                       gccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCGCATATGCCCCACATCCCGCCGCCCCCCGGCCATATTGCACCGCGCGTTCAGCCCCTGGCGGCCAATACTGTCTTCCCCCCGATAAAttt                                                >  3:1792609/1‑151 (MQ=255)
                                                                                                       gccgcTGCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAttt                                                <  3:620664/151‑1 (MQ=255)
                                                                                                        ccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCAACCCCCGCATAAGCACCACAAACACCCCCCAACCCTCAAAAATACACCGCCCATTAAGCCACTTGCGGAAAAAAAAGTCATACCCCCCAAAAAttt                                                >  2:302927/1‑150 (MQ=255)
                                                                                                         cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttca                                              <  4:2139725/151‑1 (MQ=255)
                                                                                                            ttCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCGCATATTGACCACATACACCCCCCACCCGTCCAAATTACACCGCGCGTTCAGCCACTGGCGGGCCATACAGTCATGCCCGCGATAAaattaatt                                            >  6:859064/1‑150 (MQ=255)
                                                                                                             tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATCCCGCCGCCAGCCGTCCATATTGCACGGCGGTTTGAGCCAGTGGCGGACAATACTGTCGTGCCCGCGCTAAAAttcattg                                           >  6:1214270/1‑149 (MQ=255)
                                                                                                             tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattc                                           <  4:518631/150‑1 (MQ=255)
                                                                                                             tCGGGGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattc                                           <  5:220140/150‑1 (MQ=255)
                                                                                                              cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGCATATGCACCACATCCACCCCCCCGCCCGCCAAATTACACCGCGCGTTGACCCAGGGGCGGCCAATACTGTGTTCCCCGCGCTAAATTTTATTGAc                                         >  7:1827951/1‑151 (MQ=255)
                                                                                                              cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                          <  3:1237093/150‑1 (MQ=255)
                                                                                                                 tGATCCCGGCTACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAATTCATTCACCac                                      >  2:1009264/1‑150 (MQ=255)
                                                                                                                  gATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCGCCGCCCCCCGCATATGCCCCACCTACACCCGCCCGCCGTGCATATTACCCGGCGCGTTGAGCCAGTGGCGGCCCATACGGTGTTGCCCGCGCTAAATTTTATTCACCAgc                                     >  1:1585638/1‑150 (MQ=255)
                                                                                                                  caTCCCCGCAACCTCCTCCATATAATTCCCCGCCCCACCCGCCGCCCCCCGCATCTGCACCCCATCCAGCCGCCAGCCGTCCATATTCCACCGCGCTTTCAGCCAGTGGCGGACAATCCTGGCCTCCCCGCGATAAATTCCATTCACCCGa                                     <  8:236493/150‑1 (MQ=255)
                                                                                                                   aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                    <  1:2061949/151‑1 (MQ=255)
                                                                                                                   aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                    <  1:1169373/151‑1 (MQ=255)
                                                                                                                      ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                 <  3:401432/151‑1 (MQ=255)
                                                                                                                      ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                 <  2:2190969/151‑1 (MQ=255)
                                                                                                                       cGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCCCATCCAGCCGCCAGCCGGCCATATTCCACCGCGCGTTAAGCCAGTGGCGGACCAAACTGTCTTCCCCCCGATTAATTTCATTCACCAGCCATTc                                >  6:179783/1‑151 (MQ=255)
                                                                                                                         gCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCACCATATGCCCCACATCCAGCCCCCACCCCTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAAAACTGTCTTGCCCGCGATAAATTTAATTAACCAGACATTACg                              >  8:1817852/1‑151 (MQ=255)
                                                                                                                          cAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATACAGCCGCCAGCCGTCCATATTACACGGCGCTTTCCGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCgc                             >  6:639003/1‑150 (MQ=255)
                                                                                                                          cAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACAACCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCCATAAAATTCATTCACCAGACTTTCCg                              >  2:1734440/1‑150 (MQ=255)
                                                                                                                          cAACGTGCTGCATAGTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGa                             <  4:2214984/151‑1 (MQ=255)
                                                                                                                           aaCGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGAc                            >  4:2025810/1‑151 (MQ=255)
                                                                                                                             cGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTg                          <  5:74995/151‑1 (MQ=255)
                                                                                                                               tgctgcATATTATTGCGCGCGCCCCCCGCCGCCCCCAGCATATGGCCCACATCCCGCCGCCCGGCGGGCATATTTCACGGCGCGTTGAGCCAGTGGCGGGCAATTCTGTGTTGCCCGCGTTAAATTTGATTACCCACACTTTTCGACGGAt                        >  1:1941158/1‑151 (MQ=255)
                                                                                                                               tgctgcATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATAAGCACCACATCCAGCCGCCCGCCGTCCCTATTACACGGCGCTTTCACCCAGTGGCGGACAATACTGTGATCCCCGCGCTAAATTTCATTCACAAGACGTTCCGCCTGAt                        >  6:2170182/1‑151 (MQ=255)
                                                                                                                                gctgcATATTATTGCGCGCCCCCCCCGCCTCCCCCACCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTACCCGCGATAAATTTCATTCACCAGACTTTACGACTGAt                        >  1:544681/1‑150 (MQ=255)
                                                                                                                                 ctgcATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGGGGACAATAATGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATa                       >  4:1740919/1‑150 (MQ=255)
                                                                                                                                  tgcATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACAACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCACCCAGTGGCGGACCATACTGTCTTGCCCGCGATAAATTTCATTCCCCCGACTTTCCGACTGATaa                      >  5:1160028/1‑150 (MQ=255)
                                                                                                                                   gcATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttca                                              <  7:2219402/125‑1 (MQ=255)
                                                                                                                                    cATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATCCACCCCCCAGCCGTCCATATTCCACCGCCCTTTCAGCCAGTGGCCGCCAATACTGTCTTCCCCGCCATAAATTTCATTCACCAGCCTTTCCGCCTGATAATcc                   >  3:217634/1‑151 (MQ=255)
                                                                                                                                      aattattGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAg                 <  1:460684/150‑1 (MQ=255)
                                                                                                                                        ttattGCGCTCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCGAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGTACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGc                <  3:914934/150‑1 (MQ=255)
                                                                                                                                          attGCGCGCCCCACCCCCCGCCCCCAGCAGATGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCGTTCAGCCAGTGGCGGCCAAAACTGGGTTGCCCGCGATAAAATTCATTCCCCAGACGTTACGCCTGATAAACCAgggtg             >  8:1507602/1‑147 (MQ=255)
                                                                                                                                              cgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCa         <  2:1257802/151‑1 (MQ=255)
                                                                                                                                                 gcCCCCCCCCCCTCCCCCCGCATATGCACCACATCCAGCCGCCCCCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAATTCAATCACCAGACTTTCCGACAGATAATCCAGCTTCGGCAAGc      >  6:1668977/1‑151 (MQ=255)
                                                                                                                                                 gcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGc      <  6:999375/151‑1 (MQ=255)
                                                                                                                                                    ccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTgg   >  4:1158050/1‑151 (MQ=255)
                                                                                                                                                     cACCCGCCGCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTGCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  <  8:645393/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447253‑3447552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑