BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,448,523	0	A	C	25.9%	1002.5 / 98.3	476	intergenic (‑83/+73)	malZ/proY	alpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand): ref base A (258/91); new base C (0/122); total (258/218)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 509 total aligned reads displayed.

ATGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGA  >  DH1/3448373‑3448672

   aGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagg                                                                                                                                                     >  3:217430/1‑150 (MQ=255)

   aGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagg                                                                                                                                                     >  1:1599712/1‑150 (MQ=255)

    gCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc                                                                                                                                                   <  3:201933/151‑1 (MQ=255)

    gCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc                                                                                                                                                   <  7:1579441/151‑1 (MQ=255)

     cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                  >  1:2014708/1‑151 (MQ=255)

      aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgg                                                                                                                                                 >  2:92804/1‑151 (MQ=255)

       gTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg                                                                                                                                                <  4:2173150/151‑1 (MQ=255)

       gTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg                                                                                                                                                <  5:704572/151‑1 (MQ=255)

        ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg                                                                                                                                                <  6:361094/150‑1 (MQ=255)

         tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTT‑‑‑GGAGGAGGACGGGGGCg                                                                                                                                            <  1:235224/150‑1 (MQ=255)

          gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                             >  5:2190743/1‑151 (MQ=255)

           aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCg                                                                                                                                            >  1:429837/1‑151 (MQ=255)

              tttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAt                                                                                                                                         >  5:1584438/1‑151 (MQ=255)

                   tttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACtt                                                                                                                                    >  4:1785993/1‑151 (MQ=255)

                     tgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTc                                                                                                                                  >  1:736457/1‑151 (MQ=255)

                      gTTTAACAATTGGGGGCACCGGCAGGTGCCATGAATTTAACATCATAATTCCCCTGTGATTAAACTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCGGGGGGCGGATAACTTTc                                                                                                                                  <  1:1427487/150‑1 (MQ=255)

                      gTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCg                                                                                                                                 >  4:1366428/1‑151 (MQ=255)

                       ttacacccctGGGCGCCCCGGCATGTCCCATCCATTTAACATCATAATTCCCCTGTCATCAAAATCTGACCACCTTCCCATACCCTAATCCACCATCCCTCCTCATCTGACGGATCTTTGGGGCCGGCGGCCGCCCCCGGATAACTTTCg                                                                                                                                 <  6:1035693/141‑1 (MQ=255)

                        ttaacactggggggCACCGCGCGGGGCAATTCATATAAAATCATAACTCCCCTGTTATCAAACGCTGATCCCCTTCCGATAGCCTACTGCAGGATCAGTCTACATCTGAATGATATTGGGGGGAGGCGGACGGGGGCGGATAACTTTCgg                                                                                                                                <  4:990065/141‑1 (MQ=255)

                        ttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCGGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                >  7:1123106/1‑150 (MQ=255)

                          aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                             >  3:61053/1‑151 (MQ=255)

                            aaaaTGGGGGCACCGGCAGGTGCCATTAATTTAACATAATAATTCCCCTTTGATTAAAATCTCATCACCTTGCCATAGCCTAATGCAGTATCACTCATCATCTTAATTATTTTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAg                                                                                                                           <  5:1944130/150‑1 (MQ=255)

                                tGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCgg                                                                                                                       >  4:519121/1‑151 (MQ=255)

                                  gggcCACCGGCAGGGGCCATGAATTAAACATCATAATTCCCCTGTGATGAAAATCTGAGCACCTTGCCATAGCCTAATGCAGGATCCCTCATCATCTGAAGCATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGAt                                                                                                                     <  5:1345129/151‑1 (MQ=255)

                                   gggCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATc                                                                                                                    >  2:339213/1‑151 (MQ=255)

                                   ggccACCGGGAGGTGCCATTCAATAAACATCAAAATTCCCCTCGCATGAAACTCTGATCACCTTGCCATACCCTAATGCAGCATCACTCATCATGTGACGGATATGGGGGGGAGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATc                                                                                                                    <  1:1263239/148‑1 (MQ=255)

                                    gtcACCGGCAGGTGCCATTAATTTAACATCATAATTCCCCTGTTATGAAAATCTGATCACCTTTCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGTTATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                   <  8:57102/149‑1 (MQ=255)

                                    ggCCCCGGCAGCTGACATGAATTAAACATAATAAATGCCCTCTTATCAAAATCTGATCACCTTGCCTTACCCTAATGCATTATCACTCATCATCTGATGCATATTGGGGGGAGGAGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                   <  8:1041477/151‑1 (MQ=255)

                                       aCCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg                                                                                                                 >  3:1445641/1‑150 (MQ=255)

                                        ccGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                               >  5:162137/1‑151 (MQ=255)

                                          ggCAGGTGCAATGAATTAAACATCATAATTCCCCTGTGATTAAAATCTGATCCCCTTGCCATACCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                             <  3:1681505/151‑1 (MQ=255)

                                             aGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                           >  2:103087/1‑150 (MQ=255)

                                              ggTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctc                                                                                                         >  4:338970/1‑151 (MQ=255)

                                               gTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATATGATCACCTTGCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCAGGATCAGGATTtcctcc                                                                                                        >  6:1911437/1‑151 (MQ=255)

                                                tGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                       >  3:987980/1‑151 (MQ=255)

                                                 gCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctcc                                                                                                        >  7:1793524/1‑149 (MQ=255)

                                                  caattcATTAAAAATAATAATTCGCTTTTTATTAAATTCTGCTCACTTTTCAATAGCCTAATCCATGTTCACTGATCTTCTTAAGTTTTTTTGGGGGTGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                      <  2:2270668/145‑1 (MQ=255)

                                                    aTGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                   >  5:1406430/1‑151 (MQ=255)

                                                    aTGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                   >  7:2103670/1‑151 (MQ=255)

                                                    aTGCATTAAACATCATAATGCCACTATGACGAAATTCTGAAAACGTTCACATAGCCTAATGGAGGATCAATCATCATCTCCAGGATATTTGGGGGAGGAGGACGCCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                   <  5:363616/151‑1 (MQ=255)

                                                      gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                 >  1:2187106/1‑151 (MQ=255)

                                                       cATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCgg                                                                                                >  8:273491/1‑151 (MQ=255)

                                                       cATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTCCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCgg                                                                                                >  5:464613/1‑151 (MQ=255)

                                                        aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCggg                                                                                               >  8:1307010/1‑150 (MQ=255)

                                                           tAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGACCCGGCtt                                                                                             >  4:675403/1‑150 (MQ=255)

                                                           tAACATCATAATTCCCCTGTGATGAAAAGCGGAGCACCTTGCCATAGCCTAATGCAGCAGCACTCATCATCTGAAGGATATTTGGCGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCtt                                                                                             <  5:1153322/150‑1 (MQ=255)

                                                            aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                           >  7:372268/1‑151 (MQ=255)

                                                              catcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                           >  6:2223213/1‑149 (MQ=255)

                                                               atcatAAGTCCCCTTGTATCAAAAGCTCATCACCTAGCCATAGCCTAAGCCCCCATCACTCATCCTCTGAAGGATATTTGGGGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                        <  3:1905411/151‑1 (MQ=255)

                                                                acaaacttCCCCAGTGATCAAAATCGGCGCCCCTTCCCATCCCCTACGCCAGGCTCACGCCCCAGCTGAAGTATATTGGGGGGAGGCGCACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGaa                                                                                       <  7:1682482/145‑1 (MQ=255)

                                                                 catAATGCCCCAGTCCTGAAACGCTGCTCACCTCGCCCTACCCACCAGCCGGAACACTCAACATCTGACGGATCTGTGGGGGCCGAGCCCCGCGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                      <  1:1168211/151‑1 (MQ=255)

                                                                  aaccttCCCCTCTCATCAAACTCTCCTCACCCTGCCATAACCTATTTCACCCTCCCTCATCAGCTGAACGCTCGGTGGGGGCGCCGGCCCGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACg                                                                                     <  8:496445/147‑1 (MQ=255)

                                                                   tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                    >  8:1470342/1‑151 (MQ=255)

                                                                     aTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                        >  3:1480554/1‑145 (MQ=255)

                                                                       accccTGTGCACAAACGCTGCTCCCCTGGCCCTACCCTAATGCCGCCTCCCGCCTCATCTGAAGGATATTGGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                <  5:1514750/150‑1 (MQ=255)

                                                                        ccgcTGTCATCAAACTCTGATCACCTTCCCATCCCCAACTGCAGGATCACTCATCATCTGACGGAGATGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                <  2:1545240/147‑1 (MQ=255)

                                                                        ccccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                >  1:550848/1‑150 (MQ=255)

                                                                         cccTTTGATAAAACTCTGAGCCAGTTGCCCTACCCAAATGCACGATCACTCATCATCTTAAGTATCTTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAt                                                                              <  4:20040/151‑1 (MQ=255)

                                                                             gtgATGAAATTCTCATCCCCTTGCCATACCCTAATGCAGGATCACTCATCATCTGACGGATTTTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa                                                                            <  8:1879223/149‑1 (MQ=255)

                                                                               catgaAAATCTGATCACCAAGCCATACCCAAATGCAGGACCACTCAGCATCTGAACGATCGGGGGGGGCGGCCGCCGGGCCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTgg                                                                        <  3:339999/150‑1 (MQ=255)

                                                                                  gaAAATCTGATCACCTTGCCATCGCCTAATGCAGGAGCACTCATCATCTGAAGGATAGTTGGGGCCGGAGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtt                                                                      <  7:1207988/150‑1 (MQ=255)

                                                                                   aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGACCGCAAGGATTACTGGtttt                                                                    >  2:1879061/1‑151 (MQ=255)

                                                                                    aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                   >  6:1274903/1‑151 (MQ=255)

                                                                                     aaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                   >  3:1355577/1‑150 (MQ=255)

                                                                                     aaTCTGAAAACCTTGCCTAATCATAATGCAGGAGCACTCATGATCTGACTGATTTTTGGGGGAGGCGGCGGGGCGCGGATAAGTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTca                                                                  <  7:743832/151‑1 (MQ=255)

                                                                                      atgtgttCACCTTGCATTAGCCTAATGCAGTATCACTCATCATCTGATGTATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                 <  8:1740870/145‑1 (MQ=255)

                                                                                        cTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                               >  1:184554/1‑151 (MQ=255)

                                                                                          gagcACCTTGCCATCGCCTAAGGCAGGATCACTCATCATCTGAAGGATAGTGGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                 <  5:950853/144‑1 (MQ=255)

                                                                                          gATCACCTGGCCATAGCCTAATGCCGGATCCCGCATCATCTGACGCATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccag                                                             <  7:529698/151‑1 (MQ=255)

                                                                                          caTCACCTTGCCATAGCCTAATCCAGGAGCACTCATCATCTGAAGGATATGTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                 <  7:584616/146‑1 (MQ=255)

                                                                                           agcACCCTGCCATAGCCTACTGCAGCATCACGCCGCATCTGAAGGATCTTGGGGCGCCGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccagc                                                            <  1:103386/149‑1 (MQ=255)

                                                                                           aTCACCTTGCCATAGCCAAATGCACCATCACGCATCATCTGAAGGATATTTGGGGGAGGACGACGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccagc                                                            <  7:229879/151‑1 (MQ=255)

                                                                                             cACCTTGCCAAACCCTACTCCCGGATCACTCATCATCTGAAGGATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                          <  6:561963/151‑1 (MQ=255)

                                                                                                cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATACCCGGATAACGATTTCCTCCTGAACAGGATTACAGAACGCAAGGATTACTCGTTATCAGCCAGCTGACg                                                       >  8:676579/1‑151 (MQ=255)

                                                                                                    ccATCGCATACTGCAGGCTCACTCATCATCTCAAGTATTGTTGGGGGAGGCCGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCg                                                   <  8:225651/151‑1 (MQ=255)

                                                                                                     caaaGCCTACTGCCGGATCCCTCATCATCTGACGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                  <  5:1784783/148‑1 (MQ=255)

                                                                                                     cATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                  >  6:1481825/1‑151 (MQ=255)

                                                                                                       tAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                >  8:2136608/1‑151 (MQ=255)

                                                                                                        aGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggc                                               >  4:1116113/1‑151 (MQ=255)

                                                                                                             aaTGCAGGATCACTCATCATCTGAAGGTTTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                           <  2:2130509/150‑1 (MQ=255)

                                                                                                                gCAGGATCACTCATCATCTGATGGTTTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                        <  6:805742/150‑1 (MQ=255)

                                                                                                                gCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                        >  6:2000971/1‑150 (MQ=255)

                                                                                                                  aGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                      >  4:1275524/1‑150 (MQ=255)

                                                                                                                  aGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                      >  3:266012/1‑150 (MQ=255)

                                                                                                                   ggAGCACTCATCCTCTGAAGTATTTTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACGGGCTTACAGAACGCAAGGATTACTGGTGTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaa                                     <  2:726610/150‑1 (MQ=255)

                                                                                                                      tCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATc                                 >  3:795520/1‑151 (MQ=255)

                                                                                                                            atcatcAGAAGCATCTTGGGGGGAGGAGGACGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                           <  5:809222/151‑1 (MQ=255)

                                                                                                                              catcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                         >  3:513212/1‑151 (MQ=255)

                                                                                                                              catcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGACAGCTGACGATCGTGGCGCCGTTTAAACATCCAGCCaa                          >  8:821245/1‑150 (MQ=255)

                                                                                                                               atcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                        >  3:1056253/1‑151 (MQ=255)

                                                                                                                                tcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                        >  5:2257665/1‑150 (MQ=255)

                                                                                                                                  tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                     >  7:1973946/1‑151 (MQ=255)

                                                                                                                                   gacggATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                    <  2:1430085/148‑1 (MQ=255)

                                                                                                                                    aaGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                     >  3:1805903/1‑149 (MQ=255)

                                                                                                                                     aGGATATGTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                  <  7:930527/151‑1 (MQ=255)

                                                                                                                                       gATATTTGGGGGAGGACGACGGCCCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                <  2:1972337/151‑1 (MQ=255)

                                                                                                                                        atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                >  2:1210478/1‑150 (MQ=255)

                                                                                                                                          atTGGGGGGCGGAGGACTGGGCCGGATAACTTTCGGAATAGCCGGATCAGTATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaa              <  8:1194071/150‑1 (MQ=255)

                                                                                                                                                     gaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgcg   >  3:1221158/1‑150 (MQ=255)

                                                                                                                                                      aggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGa  >  2:1694329/1‑150 (MQ=255)

ATGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGA  >  DH1/3448373‑3448672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`