BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,448,526	0	A	C	30.3%	799.0 / 152.4	476	intergenic (‑86/+70)	malZ/proY	alpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand): ref base A (254/75); new base C (3/140); total (258/218)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 505 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

  cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                      <  7:1165950/151‑1 (MQ=255)

   aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgg                                                                                                                                                     >  2:92804/1‑151 (MQ=255)

       gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                                 >  5:875347/1‑151 (MQ=255)

            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAt                                                                                                                                             >  7:1856147/1‑150 (MQ=255)

            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTCTGATGAAAATCTGATCACCTTGCCATACCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGAGGACGGGGGCgg                                                                                                                                               <  6:2290076/148‑1 (MQ=255)

                tttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACtt                                                                                                                                        >  4:1785993/1‑151 (MQ=255)

                tttgTTAAACTATTGGGGGCACCGGCTGGTGTCATTAATTTAAAATAATAATTCCACTGTGATGATAATTTTATGACCTTGCCATACCCTAATGCAGGATCACTCATCATCTGTATGTTATTTGGGGGAGGAGGACGGGGGCGGATAACt                                                                                                                                         <  3:580140/150‑1 (MQ=255)

                   gTTTAACAATTGGGGGCACCGGCAGGTGCCATGAATTTAACATCATAATTCCCCTGTGATTAAACTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCGGGGGGCGGATAACTTTc                                                                                                                                      <  1:1427487/150‑1 (MQ=255)

                     ttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                    >  5:1786543/1‑150 (MQ=255)

                     ttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                    >  6:883774/1‑150 (MQ=255)

                       aacaacTGGGGGCCCCGGCAGGTGCAATGCATTTAACATAATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGaa                                                                                                                                  <  7:170876/150‑1 (MQ=255)

                       aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTGCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGTGGGAGGAGGACGGGGGCGGATAACTTTCGGaa                                                                                                                                  >  7:1344960/1‑150 (MQ=255)

                        aCAATTGGGGGCACCGGCAGGTTCCATTAATTTAACATCATAATTCCGCTTTGATTAAAATCTGATCACCTTGCCATCGCCTAATGCAGGATCACTCATCATCTTATTTTTATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATa                                                                                                                                <  5:2268732/151‑1 (MQ=255)

                        aCAAATGGGGGCACCGGCAGCTGCCATCCATTTAACATCATAATTCCCCTGTTATCAAAATCTGATCACCTTGCCATAGCATAATGCCGGATCACTCATCATCTGAATGATATTTGGGGCCGGCGGACGGGGGCGGATAACTTTCGGAATa                                                                                                                                <  8:663397/151‑1 (MQ=255)

                            aTGGGGGCCCCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTTATGCTGCATCACTCATCATCTGATGTATTTTTGGGGGTGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                            <  5:973484/151‑1 (MQ=255)

                               ggggCACCTGAAGTTTCCATTAATTTAACATAATAATTCCCCTTTGATTAAAATCTTATCACCTTGCCATCGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGAt                                                                                                                         <  1:16307/151‑1 (MQ=255)

                                ggccACCGGGAGGTGCCATTCAATAAACATCAAAATTCCCCTCGCATGAAACTCTGATCACCTTGCCATACCCTAATGCAGCATCACTCATCATGTGACGGATATGGGGGGGAGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATc                                                                                                                        <  1:1263239/148‑1 (MQ=255)

                                 ggCCCCGGCAGCTGACATGAATTAAACATAATAAATGCCCTCTTATCAAAATCTGATCACCTTGCCTTACCCTAATGCATTATCACTCATCATCTGATGCATATTGGGGGGAGGAGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                       <  8:1041477/151‑1 (MQ=255)

                                 ggCACCGGGAGGTGCAATTAATTAAAAATAATAATTCCCCTGTGATCAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAATTATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                       <  3:1758884/151‑1 (MQ=255)

                                     ccGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                                   >  5:1568632/1‑151 (MQ=255)

                                      cgtctctttctattcATTTAACTTCATTATTGCCGTTTTTTCTATATCTGATCTGCTTCCCCTAGCTTATTGCCGTATCTCTCATCTTCTGTTGGATTTTTCGGGCGGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGAta                                                                                                                  <  2:1922605/137‑2 (MQ=255)

                                       ggCAGGTTCCATTCATTTAACATCATAATTCCCATGTGATGAAAATCTCATCACCTTCCCATAGCCTAATGCAGGATCACTCATCCTCTGACTGATATGGGGGGGCGGCGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                                 <  7:1627197/151‑1 (MQ=255)

                                         cAGGTGCCATTCATTAAACATCATAATTCCCCTGTGATGAAACTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGACGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                               <  3:16379/151‑1 (MQ=255)

                                          aGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                               >  2:103087/1‑150 (MQ=255)

                                            gTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAGTTTCGGAATAGCCGGATCAGGATTtccgcc                                                                                                            >  6:2102244/1‑151 (MQ=255)

                                             tGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                           >  3:1356923/1‑151 (MQ=255)

                                                cATGCATTTAACATAATAATTCCCCTGTTATGAAAATCTGATCCCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTTAAGTATTTTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGaa                                                                                                        <  8:563917/151‑1 (MQ=255)

                                                   tcATTAAACATCATAATTCCCCTTTCATCAAAATCTCCTCACCTTGCCATCCCCTAATCCAGGATCACTCATCATCTGAAGCATATTTGGGGGCGGCGCCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                      <  4:313597/149‑1 (MQ=255)

                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGTAGGACGGGGGCGGATAACTTTCGGAATAGCAGGATCAGGATTTCCTCCTGACCCGGc                                                                                                   >  3:235909/1‑151 (MQ=255)

                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCggg                                                                                                   >  8:1307010/1‑150 (MQ=255)

                                                      tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                                  >  6:412298/1‑151 (MQ=255)

                                                         aaCCTCATACTTCCCCTGGGATGAAAATCGGATCACCTGGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                               <  5:772465/151‑1 (MQ=255)

                                                         aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                               >  7:372268/1‑151 (MQ=255)

                                                              catAATGCCCCAGTCCTGAAACGCTGCTCACCTCGCCCTACCCACCAGCCGGAACACTCAACATCTGACGGATCTGTGGGGGCCGAGCCCCGCGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  1:1168211/151‑1 (MQ=255)

                                                              catAAAGCCCCTCTGATGAAAAGCTGATCACGTTCCCAAACCCTAATGCCGGAGCACCCATCATCTGAAGGATATTGGGGGGCGGAGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  3:1985393/151‑1 (MQ=255)

                                                              aaacactCCCCTCTCATCAAAATCTCACCACCCAGCCACCCCCTAAGCCACGACCACTCATCATCTGACGGATATGGGGGGGCGGCGGCCCGCCCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  8:42085/145‑1 (MQ=255)

                                                                tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAActc                                                                                        >  1:740836/1‑149 (MQ=255)

                                                                tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                        >  8:1470342/1‑151 (MQ=255)

                                                                tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                        >  1:1849705/1‑151 (MQ=255)

                                                                   ttCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCCTCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTGACAGAACGCa                                                                                       >  2:358766/1‑149 (MQ=255)

                                                                     ccgcTGTCATCAAACTCTGATCACCTTCCCATCCCCAACTGCAGGATCACTCATCATCTGACGGAGATGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                    <  2:1545240/147‑1 (MQ=255)

                                                                     ccccTGTTATGAAAATCTTATCACCTTGCCAAACCCTAATGCAGGATCACTCATCATGTGATGGATATTTGGGGGAGGAGGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGa                                                                                   <  4:957089/151‑1 (MQ=255)

                                                                      cccTTTGATAAAACTCTGAGCCAGTTGCCCTACCCAAATGCACGATCACTCATCATCTTAAGTATCTTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAt                                                                                  <  4:20040/151‑1 (MQ=255)

                                                                       ccTGTGATGAAAATCGGACCACCTTCCCATACCCTACTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAtt                                                                                 <  5:580212/151‑1 (MQ=255)

                                                                          gtgATGAAATTCTCATCCCCTTGCCATACCCTAATGCAGGATCACTCATCATCTGACGGATTTTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa                                                                                <  8:1879223/149‑1 (MQ=255)

                                                                           tgatgaAAATCTGATCCCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACt                                                                              <  6:1335626/150‑1 (MQ=255)

                                                                           tgatgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTg                                                                             >  6:626686/1‑151 (MQ=255)

                                                                                aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGACCGCAAGGATTACTGGtttt                                                                        >  2:1879061/1‑151 (MQ=255)

                                                                                aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                        >  3:1243063/1‑151 (MQ=255)

                                                                                aaaaTCTGATCACCTTGCCATACCATATTGCAGGATCACCCATCATCTGAAGGTTATTGGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                        <  1:2191468/151‑1 (MQ=255)

                                                                                 aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                       >  3:1467699/1‑151 (MQ=255)

                                                                                 aaaGCTGAGCACCTTGCCATAGCCTAATGCAGGACCACTCATCATCTCAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGCAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                       <  5:1364316/151‑1 (MQ=255)

                                                                                  aaTCTTATCACCTTCCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTca                                                                      <  4:62100/151‑1 (MQ=255)

                                                                                  aaTCTGAAAACCTTGCCTAATCATAATGCAGGAGCACTCATGATCTGACTGATTTTTGGGGGAGGCGGCGGGGCGCGGATAAGTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTca                                                                      <  7:743832/151‑1 (MQ=255)

                                                                                   atgtgttCACCTTGCATTAGCCTAATGCAGTATCACTCATCATCTGATGTATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                     <  8:1740870/145‑1 (MQ=255)

                                                                                      tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                   >  8:74567/1‑150 (MQ=255)

                                                                                       gATCACCTGGCCATAGCCTAATGCCGGATCCCGCATCATCTGACGCATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccag                                                                 <  7:529698/151‑1 (MQ=255)

                                                                                       caTCACCTTGCCATAGCCTAATCCAGGAGCACTCATCATCTGAAGGATATGTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                     <  7:584616/146‑1 (MQ=255)

                                                                                        agcACCCTGCCATAGCCTACTGCAGCATCACGCCGCATCTGAAGGATCTTGGGGCGCCGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccagc                                                                <  1:103386/149‑1 (MQ=255)

                                                                                        aTCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATATCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccagc                                                                >  1:677209/1‑151 (MQ=255)

                                                                                         ccACCTTCCCATAGCCTAATGCAGGATCACGCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                               <  5:2056865/150‑1 (MQ=255)

                                                                                          cACCTTGACATACCCTAATGGCGGATAACTCATCATCTGCAGGCTTGTTGGGGGCGGCGGCCGCCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAG‑CAGCTg                                                              <  4:1081461/150‑1 (MQ=255)

                                                                                                gCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                        >  1:253240/1‑151 (MQ=255)

                                                                                                       ccTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcgg                                                 >  8:263859/1‑151 (MQ=255)

                                                                                                        cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                >  3:48770/1‑151 (MQ=255)

                                                                                                        cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCGGAACCGGCTTACAGAACGGAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                >  4:135985/1‑151 (MQ=255)

                                                                                                          aaTGCAGGATCACTCATCATCTGAAGGTTTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                               <  2:2130509/150‑1 (MQ=255)

                                                                                                          aaTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGt                                              >  5:1452145/1‑151 (MQ=255)

                                                                                                              cAGGATCCCTCATCCGCTGAAGGATTTGTGGGGGAGGGCGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                          <  1:2058278/151‑1 (MQ=255)

                                                                                                              cAGGAGCACTCATCCTCTGAAGGATATTGGGGGGCGGAGGCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                          <  6:1580116/151‑1 (MQ=255)

                                                                                                               cccaTCACTCATCCCCTGACGTATCGGTGGGGGAGCACCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaa                                         <  2:799583/148‑1 (MQ=255)

                                                                                                                gcaTCACTCATCATCTGACGGATATTGGGGGGAGGACGACGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc                                        <  2:2271189/149‑1 (MQ=255)

                                                                                                                 gATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGCCGATCGTGGCGGCGTTTAAAc                                        >  6:192081/1‑150 (MQ=255)

                                                                                                                  cccccTCATCAGCTGAAGGTTCTTGGGGGGAGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACAt                                      <  4:2150861/147‑1 (MQ=255)

                                                                                                                  atcaagcatcatCTGAAGGATATTGGGGCGAGGACGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACAt                                      <  5:160524/145‑1 (MQ=255)

                                                                                                                  aTCACTCATCATCTGACGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACAt                                      <  3:2120930/151‑1 (MQ=255)

                                                                                                                   tCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATc                                     >  7:1978693/1‑151 (MQ=255)

                                                                                                                    cACTCATCCTCTGAAGGATATTTGGGGGAGCAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                    <  5:404459/151‑1 (MQ=255)

                                                                                                                       gcagccgcTGAAGGATATTTGGGGGCGGCCGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGTATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGc                                 <  1:431795/144‑1 (MQ=255)

                                                                                                                        catcatCTGATGTATTTTTCGGGGAGGAGGACGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                                <  4:1264672/151‑1 (MQ=255)

                                                                                                                         atcatcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGGTTAAACATCCAGcc                                >  6:1099691/1‑150 (MQ=255)

                                                                                                                            atcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                            >  3:1056253/1‑151 (MQ=255)

                                                                                                                              cTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCa                           >  5:1266392/1‑150 (MQ=255)

                                                                                                                               tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         >  7:2227531/1‑151 (MQ=255)

                                                                                                                                gacggATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                        <  2:1430085/148‑1 (MQ=255)

                                                                                                                                gAAGGTTTTTTGGGGGGGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         <  8:2243990/150‑1 (MQ=255)

                                                                                                                                gAAGGAAAGGTGGGGGAGGAGCACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGATCGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                        <  8:2128938/151‑1 (MQ=255)

                                                                                                                                  aGGATATGTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                      <  7:930527/151‑1 (MQ=255)

                                                                                                                                     atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    >  2:1210478/1‑150 (MQ=255)

                                                                                                                                       atTGGGGGGCGGAGGACTGGGCCGGATAACTTTCGGAATAGCCGGATCAGTATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaa                  <  8:1194071/150‑1 (MQ=255)

                                                                                                                                       atTGGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaa                  <  5:1702629/150‑1 (MQ=255)

                                                                                                                                         ggggccgcgcaccaCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaac                 <  3:743037/136‑1 (MQ=255)

                                                                                                                                          tGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCAcaacaa               <  3:288758/150‑1 (MQ=255)

                                                                                                                                            ggggAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATcc            >  3:1894532/1‑151 (MQ=255)

                                                                                                                                                 ggcggaCGGGGGCGGATAACTTTCGGAATAGCCGGATGAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgcg       <  4:419825/148‑1 (MQ=255)

                                                                                                                                                 ggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgcg       <  3:874877/151‑1 (MQ=255)

                                                                                                                                                   aggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGa      >  2:1694329/1‑150 (MQ=255)

                                                                                                                                                   aggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGa      >  5:2217026/1‑150 (MQ=255)

                                                                                                                                                    ccaCGGCGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGaaa    <  7:251418/149‑1 (MQ=255)

                                                                                                                                                     gaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAc   >  5:1028793/1‑151 (MQ=255)

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`