Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 205,979 T→C D189D (GAT→GAC)  yhjG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1205,9790TC96.7% 2206.4 / 35.8 609D189D (GAT→GAC) yhjGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/11);  new base C (281/307);  total (291/318)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01

Only 100 of 614 total aligned reads displayed.

GGACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGT  >  DH1/205831‑206126
                                                                                                                                                    |                                                                                                                                                   
 gACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGAcccc                                                                                                                                                  >  4:1450561/1‑151 (MQ=255)
  aCGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCt                                                                                                                                                 >  1:1123321/1‑151 (MQ=255)
  aCGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCt                                                                                                                                                 >  5:1099087/1‑151 (MQ=255)
  aCGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTAGAGATTTTTGTTGACCCCt                                                                                                                                                 <  4:2232501/151‑1 (MQ=255)
   cGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCtt                                                                                                                                                >  7:1471539/1‑151 (MQ=255)
    gTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCtt                                                                                                                                                >  8:1053939/1‑150 (MQ=255)
      ttAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGAAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAgg                                                                                                                                             >  3:1967460/1‑151 (MQ=255)
         aTCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGCCCCCTTAGGCa                                                                                                                                           >  2:1383364/1‑150 (MQ=255)
          tCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCa                                                                                                                                           >  8:728354/1‑149 (MQ=255)
             tGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGc                                                                                                                                        <  8:894323/149‑1 (MQ=255)
             tGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCg                                                                                                                                      >  4:1923633/1‑151 (MQ=255)
                            cAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGa                                                                                                                        >  7:720486/1‑150 (MQ=255)
                             aaaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAg                                                                                                                      <  6:46994/151‑1 (MQ=255)
                              aaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGt                                                                                                                     >  1:1694628/1‑151 (MQ=255)
                              aaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGt                                                                                                                     <  1:2131097/151‑1 (MQ=255)
                              aaGACGCGAATGCAAAGCCGTCGGCATGGGCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGt                                                                                                                     <  3:168936/151‑1 (MQ=255)
                                  cgcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACt                                                                                                                 <  2:403870/151‑1 (MQ=255)
                                     gAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTgg                                                                                                               >  1:748915/1‑150 (MQ=255)
                                     gAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTgg                                                                                                               >  3:1883017/1‑150 (MQ=255)
                                      aaTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGAt                                                                                                             <  5:1335871/151‑1 (MQ=255)
                                       aTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATc                                                                                                            <  4:1072904/151‑1 (MQ=255)
                                         gCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGa                                                                                                          <  7:2118425/151‑1 (MQ=255)
                                           aaaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGaa                                                                                                         >  8:150121/1‑150 (MQ=255)
                                           aaaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGaa                                                                                                         >  1:455302/1‑150 (MQ=255)
                                             aGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAgg                                                                                                      <  5:315013/151‑1 (MQ=255)
                                              gCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGt                                                                                                     <  2:765597/151‑1 (MQ=255)
                                              gCCGTCGGCATGGTCGTTTCGGCAGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGt                                                                                                     <  4:1681228/151‑1 (MQ=255)
                                                 gTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATAGAAAGGTaa                                                                                                   >  5:2044930/1‑150 (MQ=255)
                                                   gggCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGc                                                                                                <  2:2119553/150‑1 (MQ=255)
                                                   cGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAg                                                                                                 <  3:302503/150‑1 (MQ=255)
                                                   cGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGc                                                                                                <  5:801405/151‑1 (MQ=255)
                                                      cATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACTAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGa                                                                                             <  6:2090595/151‑1 (MQ=255)
                                                       aTGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGAt                                                                                            >  4:1830091/1‑151 (MQ=255)
                                                       aTGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGAt                                                                                            >  7:1030407/1‑151 (MQ=255)
                                                         ggTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATa                                                                                           <  4:2052189/150‑1 (MQ=255)
                                                         ggTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATa                                                                                           <  1:294790/150‑1 (MQ=255)
                                                         ggTCGTTTAGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTAATGGATCGAAAGGTAAAGCGGATa                                                                                           >  6:220872/1‑150 (MQ=255)
                                                          gTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaaa                                                                                         <  1:1010129/151‑1 (MQ=255)
                                                          gTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaaa                                                                                         <  3:960359/151‑1 (MQ=255)
                                                           tCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                          >  1:383310/1‑149 (MQ=255)
                                                            cGTTTCGGCTGGATAATATTCTTTTCGATCCAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGa                                                                                       <  7:2213643/151‑1 (MQ=255)
                                                            cGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGa                                                                                       <  7:179418/151‑1 (MQ=255)
                                                              tttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaa                                                                                      >  3:685563/1‑150 (MQ=255)
                                                              tttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATATGCAGATTTTTGTTGACCCCTTAGGCAAGCCGCAGCCGTTCAGCGAAGTTACTGGATCGAAAGCTAAAGCGGATAAAGaa                                                                                      >  8:648325/1‑150 (MQ=255)
                                                               ttCGGCTGTATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaaa                                                                                     <  4:1193936/150‑1 (MQ=255)
                                                               ttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaaa                                                                                     >  3:837671/1‑150 (MQ=255)
                                                               ttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaaa                                                                                     <  6:1510229/150‑1 (MQ=255)
                                                                tCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaaaa                                                                                    <  3:1813755/150‑1 (MQ=255)
                                                                   gCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTg                                                                                >  4:589840/1‑151 (MQ=255)
                                                                     gggATAATATTCTATTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTggg                                                                              <  2:1261630/150‑1 (MQ=255)
                                                                      ggATAATTTTGTTTTCGATCAAGGGCGGATCGCCATTGTTGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTggg                                                                              <  1:924929/150‑1 (MQ=255)
                                                                      ggATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTggg                                                                              <  2:1159581/150‑1 (MQ=255)
                                                                      ggATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTggg                                                                              <  5:1923920/150‑1 (MQ=255)
                                                                           atatTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGAGGATAAAGAAAAGGTGGGCGAtt                                                                         >  2:954170/1‑150 (MQ=255)
                                                                           atatTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAtt                                                                         >  3:1392454/1‑150 (MQ=255)
                                                                             aatCTTTTCGATCATGGGCGGATCGCCTTTGATGAGAAAGTAAGCAAAGCGGATCTGGTGATTTTTGTAGACCCCTTCGGCATGCGGCTGCCGTTCAGCGTATTTAGTGGATCGATAGGTAAAGCTGATAAACAAAAGGTGGGCGTTTAc                                                                       <  6:1312054/148‑1 (MQ=255)
                                                                              ttCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGt                                                                     >  7:1929514/1‑151 (MQ=255)
                                                                                   ttCGATCAAGGGCGGATCGCAATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGttttt                                                                 <  3:2142097/150‑1 (MQ=255)
                                                                                     cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTgg                                                               <  2:1919076/150‑1 (MQ=255)
                                                                                       aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                            >  8:2063107/1‑151 (MQ=255)
                                                                                         cAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTg                                                           >  5:2205901/1‑150 (MQ=255)
                                                                                         cAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTg                                                           >  5:499217/1‑150 (MQ=255)
                                                                                          aaGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCACCGAAGTTACTGGATCGAAAGCGAAAGCGGATAAAGAAAAGGTGCGCGATTACGTATTTGGCCTGa                                                          >  5:1472816/1‑150 (MQ=255)
                                                                                             ggCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGgc                                                      <  1:616598/151‑1 (MQ=255)
                                                                                             ggCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGgc                                                      >  5:72983/1‑151 (MQ=255)
                                                                                              gCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGgcg                                                     <  3:452345/151‑1 (MQ=255)
                                                                                               gggATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCTATTACGTTTTTGGCCTGAAGgcgc                                                    <  8:1671798/150‑1 (MQ=255)
                                                                                               cGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGCGCGATTACGTTTTTGGCCTGAAgg                                                       >  7:1651013/1‑148 (MQ=255)
                                                                                                  aTCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTCTTTGGCCTGAAGGCGCAgg                                                 >  8:406657/1‑151 (MQ=255)
                                                                                                   tCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAggg                                                >  3:1483013/1‑151 (MQ=255)
                                                                                                     gCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGAc                                              <  1:2019664/151‑1 (MQ=255)
                                                                                                      ccATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACg                                             <  7:4539/151‑1 (MQ=255)
                                                                                                        aTTGATGACAAAGTAAGCAAATCGGATCTTGATATTTTTGTTGACCCCTTAGGCAATCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGat                                           <  3:1183018/151‑1 (MQ=255)
                                                                                                          tgatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGatat                                         >  1:1477360/1‑151 (MQ=255)
                                                                                                          tgatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGatat                                         >  7:1196288/1‑151 (MQ=255)
                                                                                                          tgatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGatat                                         >  4:829905/1‑151 (MQ=255)
                                                                                                          tgatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGCCAAGCCGCTGACGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGata                                          >  5:93314/1‑150 (MQ=255)
                                                                                                              gaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACg                                     >  2:1852505/1‑151 (MQ=255)
                                                                                                               aCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACg                                     <  2:262747/150‑1 (MQ=255)
                                                                                                                cAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGt                                   <  6:1865147/151‑1 (MQ=255)
                                                                                                                cAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGt                                   <  5:1803832/151‑1 (MQ=255)
                                                                                                                 aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGCCGATATAACGGTg                                  >  2:484074/1‑151 (MQ=255)
                                                                                                                   aGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGa                                 >  8:2229128/1‑150 (MQ=255)
                                                                                                                      aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCg                             >  7:238895/1‑151 (MQ=255)
                                                                                                                      aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCg                             <  6:1635602/151‑1 (MQ=255)
                                                                                                                        gCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCt                           <  1:1386807/151‑1 (MQ=255)
                                                                                                                         cAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                          >  5:2269077/1‑151 (MQ=255)
                                                                                                                           aaGCGGATCTGGCGCTTTTTGTTGCCCCCTTAGGCACGCCGCTGCCGTTCAGCGAAGTTACTGGCTCGAAAGGGACCGCGGATAAAGAAAAGGTGGGCGATTACGTGTTTGGCCTGCCGGCGCAGGGACGCTATACCGCTGCACCGcgccc                        >  6:704500/1‑147 (MQ=255)
                                                                                                                            aGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACg                       <  2:1040544/151‑1 (MQ=255)
                                                                                                                             gCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACgg                      <  6:1946168/151‑1 (MQ=255)
                                                                                                                                   cTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACgg                >  8:533507/1‑151 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAATGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACggg               <  1:1853963/150‑1 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTa                   <  6:1096968/146‑1 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACgg                <  2:1602157/149‑1 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGa              <  8:1925535/151‑1 (MQ=255)
                                                                                                                                       agaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaa             >  4:998935/1‑150 (MQ=255)
                                                                                                                                         aTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaaaaa          <  5:1632877/151‑1 (MQ=255)
                                                                                                                                         aTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaaaaa          <  2:1719299/151‑1 (MQ=255)
                                                                                                                                            tttGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGAACGGGAAAAAt         >  6:257120/1‑149 (MQ=255)
                                                                                                                                            tttGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGATTTTTGCCTGAAGGCGCAGGGACGAGATAACGGTGAACCGCTCACGCGTACGGCAAAAAt         >  8:1271035/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGT  >  DH1/205831‑206126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑