Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,140,096 T→C E180G (GAA→GGA)  purR ← PurR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,140,0960TC95.2% 1708.9 / 48.2 483E180G (GAA→GGA) purRPurR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (15/8);  new base C (259/200);  total (275/208)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 483 total aligned reads displayed.

GGCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCC  >  DH1/2139948‑2140242
                                                                                                                                                    |                                                                                                                                                  
ggCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcg                                                                                                                                                  <  5:1506189/151‑1 (MQ=255)
 gcgcGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgc                                                                                                                                                 <  3:835015/151‑1 (MQ=255)
  cgcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgcg                                                                                                                                                <  4:770304/151‑1 (MQ=255)
  cgcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgcg                                                                                                                                                >  1:317256/1‑151 (MQ=255)
    cgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGt                                                                                                                                              <  5:448940/151‑1 (MQ=255)
    cgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGt                                                                                                                                              >  6:202817/1‑151 (MQ=255)
    cgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCAAGCGGGCCGGGGATGACGCCGATTCCGCGGt                                                                                                                                              >  5:805152/1‑151 (MQ=255)
      aTAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCCCTTCTTCCATCGCCTTCATAAAACCGGCAAGCCGGCCTGCGCCGGTGTTACGTTCCCCCGGGCCGGGGATGACGCCGATTCcgcgctcc                                                                                                                                            >  4:529171/1‑147 (MQ=255)
        aaCCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGAcc                                                                                                                                          >  6:76536/1‑151 (MQ=255)
        aaCCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGAcc                                                                                                                                          >  4:982795/1‑151 (MQ=255)
                 cAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAAt                                                                                                                                 >  4:1105031/1‑151 (MQ=255)
                    gTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAg                                                                                                                              <  3:1602463/151‑1 (MQ=255)
                       cAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGAt                                                                                                                            <  1:884808/150‑1 (MQ=255)
                        aaaGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATaa                                                                                                                          <  8:190538/151‑1 (MQ=255)
                             cACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGccc                                                                                                                     <  2:664548/151‑1 (MQ=255)
                             cACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGccc                                                                                                                     >  8:2189374/1‑151 (MQ=255)
                                ccTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGc                                                                                                                  >  7:572832/1‑151 (MQ=255)
                                   gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCAt                                                                                                               >  1:1001233/1‑151 (MQ=255)
                                    cacaATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGTGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATg                                                                                                              >  3:120634/1‑151 (MQ=255)
                                    cacaATCCAGCTTACCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATg                                                                                                              >  3:567967/1‑151 (MQ=255)
                                     acaATCCAGCTTTCCGGCGCCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGAGGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGt                                                                                                             >  2:1515237/1‑151 (MQ=255)
                                       aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGt                                                                                                             >  6:2225293/1‑149 (MQ=255)
                                       aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAg                                                                                                           <  3:265470/151‑1 (MQ=255)
                                        aTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgc                                                                                                          <  1:1948932/151‑1 (MQ=255)
                                          ccAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgcc                                                                                                         <  7:1974752/150‑1 (MQ=255)
                                            aGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgccgcc                                                                                                      >  7:2255480/1‑151 (MQ=255)
                                            aGCTTTCCGCCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgccgcc                                                                                                      <  8:1663866/151‑1 (MQ=255)
                                              cTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCtt                                                                                                    >  8:1200308/1‑151 (MQ=255)
                                               tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   >  6:417208/1‑151 (MQ=255)
                                               tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   >  4:2162774/1‑151 (MQ=255)
                                                ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAACCCGGCATGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGTCGGCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   <  5:1925191/150‑1 (MQ=255)
                                                ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   <  6:2121767/150‑1 (MQ=255)
                                                ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   <  1:1875476/150‑1 (MQ=255)
                                                ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCg                                                                                                  >  4:923381/1‑151 (MQ=255)
                                                 tCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCg                                                                                                  >  8:2075855/1‑150 (MQ=255)
                                                    ggCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcg                                                                                              >  6:314389/1‑151 (MQ=255)
                                                     gCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcgc                                                                                             >  3:1682944/1‑151 (MQ=255)
                                                      cACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcgcg                                                                                            <  5:1593206/151‑1 (MQ=255)
                                                        ccTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          <  2:1804747/151‑1 (MQ=255)
                                                         cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  1:425717/1‑150 (MQ=255)
                                                           tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTa                                                                                         <  1:747927/149‑1 (MQ=255)
                                                             atcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCa                                                                                      >  6:1158133/1‑150 (MQ=255)
                                                              tcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAAt                                                                                    >  4:1757682/1‑151 (MQ=255)
                                                              tcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAAt                                                                                    >  7:1661901/1‑151 (MQ=255)
                                                               catcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATg                                                                                   >  5:1393567/1‑151 (MQ=255)
                                                               catcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATg                                                                                   >  5:2053946/1‑151 (MQ=255)
                                                                atcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGTTGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATACCGCCCGGCCATCTAGCCGCCTTCGAACGCGTTATCAATg                                                                                   >  2:459771/1‑150 (MQ=255)
                                                                 tcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAc                                                                                 >  5:506461/1‑151 (MQ=255)
                                                                  cGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAcc                                                                                >  5:1249693/1‑151 (MQ=255)
                                                                  cGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAcc                                                                                <  8:847698/151‑1 (MQ=255)
                                                                  cGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAc                                                                                 <  2:1325008/150‑1 (MQ=255)
                                                                   gCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCg                                                                               >  5:682032/1‑151 (MQ=255)
                                                                    cttcttCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGc                                                                              >  2:2235288/1‑151 (MQ=255)
                                                                     ttcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGc                                                                              >  1:2281090/1‑150 (MQ=255)
                                                                       cttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATc                                                                           >  5:2263354/1‑151 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            <  8:482638/148‑1 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            <  7:1013761/148‑1 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATc                                                                           <  8:1300084/149‑1 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCg                                                                          <  1:1734373/150‑1 (MQ=255)
                                                                           cATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTg                                                                       <  2:887513/151‑1 (MQ=255)
                                                                                ccTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTc                                                                  >  7:93842/1‑151 (MQ=255)
                                                                                   tCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAg                                                                <  3:1263268/150‑1 (MQ=255)
                                                                                    cATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCt                                                              >  1:124423/1‑151 (MQ=255)
                                                                                     aTAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGCCGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTACCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCtt                                                             >  1:811545/1‑151 (MQ=255)
                                                                                     aTAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATATCGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCtt                                                             <  1:2129946/151‑1 (MQ=255)
                                                                                     aTAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCtt                                                             >  2:1718209/1‑151 (MQ=255)
                                                                                              gCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCAc                                                    <  7:851225/151‑1 (MQ=255)
                                                                                                  ggcggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCa                                                <  4:1862809/151‑1 (MQ=255)
                                                                                                  ggcggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCa                                                <  8:1450225/151‑1 (MQ=255)
                                                                                                    cggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGt                                              >  3:452661/1‑151 (MQ=255)
                                                                                                     ggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTc                                             >  6:910408/1‑151 (MQ=255)
                                                                                                      gcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTcc                                            >  5:936495/1‑151 (MQ=255)
                                                                                                      gcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGCTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTcc                                            >  2:1200164/1‑151 (MQ=255)
                                                                                                           cgcCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGac                                       >  7:2189722/1‑151 (MQ=255)
                                                                                                             ccGGTGTTACGTTCCAGCGGGCCGGTGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGacca                                     <  7:1539/151‑1 (MQ=255)
                                                                                                               ggTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccacc                                   >  1:1113003/1‑151 (MQ=255)
                                                                                                               ggTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccacc                                   >  5:1845561/1‑151 (MQ=255)
                                                                                                                  gtTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCAt                                 <  7:1217676/150‑1 (MQ=255)
                                                                                                                    tACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTgg                              >  3:1516054/1‑151 (MQ=255)
                                                                                                                    tACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTg                               <  1:1364992/150‑1 (MQ=255)
                                                                                                                     aCGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTggg                             <  3:1966616/151‑1 (MQ=255)
                                                                                                                     aCGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTgg                              <  8:298249/150‑1 (MQ=255)
                                                                                                                        ttCCAGCGCGCCGGGGATGACGCCGATTGCGCTGTGACCGCGTTGAATCAGATAACGCCAGGACATGTAGACGCCTTCGAACGCCTTATCAATGACCGCTTCGGTCAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGat                           >  6:1640847/1‑150 (MQ=255)
                                                                                                                          ccAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATg                        <  8:693923/151‑1 (MQ=255)
                                                                                                                            aGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGc                      <  6:1413763/151‑1 (MQ=255)
                                                                                                                                ggCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGAt                   >  7:176908/1‑150 (MQ=255)
                                                                                                                                  ccGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATTACGCCCGGCCATGTAGCCGCCTTCGAACGCGGTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATAct                <  4:1413547/151‑1 (MQ=255)
                                                                                                                                    ggggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActct              >  4:483731/1‑151 (MQ=255)
                                                                                                                                    ggggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActct              <  5:314991/151‑1 (MQ=255)
                                                                                                                                        aTGACGCCGATTCGGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCa          <  6:1055175/151‑1 (MQ=255)
                                                                                                                                        aTGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCa          <  3:2001454/151‑1 (MQ=255)
                                                                                                                                        aTGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCa          <  7:340915/151‑1 (MQ=255)
                                                                                                                                          gACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCAGCATTGGGATATGGCGATACTCTTCCAg         <  2:748759/150‑1 (MQ=255)
                                                                                                                                          gACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAg         <  6:610208/150‑1 (MQ=255)
                                                                                                                                          gACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGc        <  2:316585/151‑1 (MQ=255)
                                                                                                                                             gCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATc     <  3:715051/151‑1 (MQ=255)
                                                                                                                                              ccGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCg    >  4:163024/1‑151 (MQ=255)
                                                                                                                                              ccGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCg    <  4:346051/151‑1 (MQ=255)
                                                                                                                                               cGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGc   >  2:1411864/1‑151 (MQ=255)
                                                                                                                                                gATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGcc  <  8:2203811/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCC  >  DH1/2139948‑2140242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑