Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,547,816 A→G Y80H (TAC→CAC)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,8160AG95.5% 1988.2 / 50.4 560Y80H (TAC→CAC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (10/15);  new base G (281/253);  total (292/268)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 560 total aligned reads displayed.

TGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547669‑4547963
                                                                                                                                                   |                                                                                                                                                   
tgCGGGCGTTTTCAGGTTTGTCGAAGTTTACTTCGTTAACTTTCAGGAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCAGCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGa                                                                                                                                                   <  3:1325592/150‑1 (MQ=255)
      cGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  8:726633/1‑151 (MQ=255)
       gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  8:839414/1‑150 (MQ=255)
       gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  3:1123203/1‑150 (MQ=255)
       gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  7:2220690/1‑150 (MQ=255)
         tttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGGGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAgg                                                                                                                                         >  5:333242/1‑151 (MQ=255)
          ttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAggc                                                                                                                                        >  7:856341/1‑150 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGCAACGGCGGATTTGGCTAGGGGAAACGAGGATGTGATCAGGccc                                                                                                                                      >  2:1698283/1‑151 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGAc                                                                                                                                       <  1:2253162/150‑1 (MQ=255)
             aGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCg                                                                                                                                     <  8:522097/151‑1 (MQ=255)
                  tGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAATCGTGGATGTCATCAGGGCCGGCg                                                                                                                                  >  5:148944/1‑149 (MQ=255)
                     cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGt                                                                                                                              >  4:2131651/1‑150 (MQ=255)
                     cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTa                                                                                                                             <  2:1511585/151‑1 (MQ=255)
                        aGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGa                                                                                                                          <  8:1210169/151‑1 (MQ=255)
                          ttAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGc                                                                                                                        >  6:284349/1‑151 (MQ=255)
                           tAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGc                                                                                                                        <  5:2196552/150‑1 (MQ=255)
                                  gTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCg                                                                                                                >  4:136173/1‑151 (MQ=255)
                                   ttAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                               >  7:505759/1‑151 (MQ=255)
                                   ttAACTTTAAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                               >  7:1690609/1‑151 (MQ=255)
                                    tAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                               <  2:1387261/150‑1 (MQ=255)
                                    tAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTACGAGCTGTCTGCgg                                                                                                               >  1:2174636/1‑150 (MQ=255)
                                     aaCTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGa                                                                                                              >  6:1795709/1‑150 (MQ=255)
                                     aaCTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGaa                                                                                                             <  6:716809/151‑1 (MQ=255)
                                      aCTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAAc                                                                                                            <  3:151063/151‑1 (MQ=255)
                                         ttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                          >  2:313145/1‑150 (MQ=255)
                                         ttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACg                                                                                                           <  2:436412/149‑1 (MQ=255)
                                           cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                       <  6:578033/151‑1 (MQ=255)
                                           cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                       >  7:2020904/1‑151 (MQ=255)
                                              cagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGGTGAAACGGCGGATTTGGCTAGGGGAAACGTGGAGGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGaaa                                                                                                    <  2:1184686/151‑1 (MQ=255)
                                                gcgcAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAAcc                                                                                                  <  1:1003113/151‑1 (MQ=255)
                                                   cAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCaa                                                                                                >  3:603975/1‑150 (MQ=255)
                                                    aaaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCaact                                                                                              >  1:1755822/1‑149 (MQ=255)
                                                    aaaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAat                                                                                              >  6:99103/1‑151 (MQ=255)
                                                       aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcca                                                                                           <  4:1952235/151‑1 (MQ=255)
                                                           cGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCt                                                                                       >  4:638071/1‑151 (MQ=255)
                                                           cGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCt                                                                                       >  8:2064677/1‑151 (MQ=255)
                                                            gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                      >  7:131257/1‑151 (MQ=255)
                                                            gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                      <  1:2048516/151‑1 (MQ=255)
                                                            gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                      <  2:781130/151‑1 (MQ=255)
                                                            gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGGAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                      >  3:1700844/1‑151 (MQ=255)
                                                            gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                      <  8:1876029/151‑1 (MQ=255)
                                                               cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGCCCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCa                                                                                    <  5:503369/150‑1 (MQ=255)
                                                                 ccTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAata                                                                                 <  2:708891/151‑1 (MQ=255)
                                                                      tttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAACTCCCTCCTGCAATATCTcc                                                                            >  3:32916/1‑151 (MQ=255)
                                                                       ttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAtct                                                                              <  1:581680/148‑1 (MQ=255)
                                                                        tCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCa                                                                           <  1:761719/150‑1 (MQ=255)
                                                                          ggcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTa                                                                        <  6:1493386/151‑1 (MQ=255)
                                                                           gcgggcgATTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTAc                                                                       <  1:416380/151‑1 (MQ=255)
                                                                           gcgggcgAATCTTAGCAGAGATGGTATCACGAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTAc                                                                       <  2:1016810/151‑1 (MQ=255)
                                                                           gcgggcgAATCTTACCAGAGATGGTATCACGAGTGCGGAGGTTTAAACGGCGTATTTGGCTAGGTTATACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCTGAACTGAGGAAACCAAATCCATCCTGCAATATCTCCATTAc                                                                       <  7:1138336/151‑1 (MQ=255)
                                                                             gggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGc                                                                     >  6:1155254/1‑151 (MQ=255)
                                                                              ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                    <  1:1121577/151‑1 (MQ=255)
                                                                              ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                    >  3:683445/1‑151 (MQ=255)
                                                                               gcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                    >  7:1891230/1‑150 (MQ=255)
                                                                               gcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCa                                                                   <  2:2059846/151‑1 (MQ=255)
                                                                                cgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCAt                                                                  <  2:245384/151‑1 (MQ=255)
                                                                                 gAATCTTACAAGAGATGGTATCACCAGTGCGGAGGTTGAAACCGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCCGCGACGTAGGAGCTGTCTGCTGAACGGAGGAAACCAAATCCATCGTGCAATATCTCCAGTACGCCATc                                                                 >  4:1239137/1‑151 (MQ=255)
                                                                                  aaTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCa                                                                <  5:253755/151‑1 (MQ=255)
                                                                                   aTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCAc                                                               >  6:2102589/1‑151 (MQ=255)
                                                                                       tACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                            >  6:1734772/1‑150 (MQ=255)
                                                                                        aCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAg                                                          <  5:43055/151‑1 (MQ=255)
                                                                                        aCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAg                                                          <  6:14087/151‑1 (MQ=255)
                                                                                         ccAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGa                                                         <  5:1678628/151‑1 (MQ=255)
                                                                                          cAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                            >  5:1899260/1‑147 (MQ=255)
                                                                                            gagaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACGGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGatat                                                      <  7:1467050/151‑1 (MQ=255)
                                                                                             agaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATc                                                     >  6:448524/1‑151 (MQ=255)
                                                                                              gaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCt                                                    >  4:1814499/1‑151 (MQ=255)
                                                                                              gaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCt                                                    <  6:1929532/151‑1 (MQ=255)
                                                                                               aTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCtt                                                   <  1:1906525/151‑1 (MQ=255)
                                                                                               aTGCTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTATGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCGATCACCAAAGATATCtt                                                   <  8:1674991/151‑1 (MQ=255)
                                                                                                 ggTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTc                                                  <  5:592362/150‑1 (MQ=255)
                                                                                                      cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAc                                             <  7:312887/150‑1 (MQ=255)
                                                                                                       aCCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCActc                                           <  4:810703/151‑1 (MQ=255)
                                                                                                         cactGCGGATGTTCAAACGGCGCATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCCGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGACATCAACAAAGATATCTTCGCCACTCtt                                         >  2:2190510/4‑151 (MQ=255)
                                                                                                         cAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGTTGTCATCAGGACCGGCGAGGTAGGAGCTGGCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTGCAGTACGCCATCACCAAAGATATCTTCGCCACTCtt                                         <  4:461376/151‑1 (MQ=255)
                                                                                                         cAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCtt                                         <  8:58899/151‑1 (MQ=255)
                                                                                                           gTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTg                                       >  3:572438/1‑151 (MQ=255)
                                                                                                             gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCg                                     >  8:1124294/1‑151 (MQ=255)
                                                                                                                 aGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGATGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgct                                 <  8:1409767/151‑1 (MQ=255)
                                                                                                                   gTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgctgc                               <  5:1799034/151‑1 (MQ=255)
                                                                                                                      gAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                            >  1:1605415/1‑151 (MQ=255)
                                                                                                                        aaCGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCGGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAg                          <  2:987109/151‑1 (MQ=255)
                                                                                                                        aaCGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                            >  3:853527/1‑149 (MQ=255)
                                                                                                                        aaCGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAg                          <  3:2109297/151‑1 (MQ=255)
                                                                                                                         aCGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAgg                         <  1:243007/151‑1 (MQ=255)
                                                                                                                         aCGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAgg                         >  2:1978568/1‑151 (MQ=255)
                                                                                                                          cggcggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGGGATGTAGGAGCTGTCTGCGGAACGGATGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGa                        <  4:1018300/151‑1 (MQ=255)
                                                                                                                           ggcggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       >  5:902515/1‑151 (MQ=255)
                                                                                                                             cggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATgg                     >  5:803118/1‑151 (MQ=255)
                                                                                                                              ggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGc                    <  8:432196/151‑1 (MQ=255)
                                                                                                                               gATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGc                    >  5:1242056/1‑150 (MQ=255)
                                                                                                                                  ttGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                 >  1:1422402/1‑150 (MQ=255)
                                                                                                                                  ttGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                 <  3:1905301/150‑1 (MQ=255)
                                                                                                                                    ggCTAGGGGAAACGTGCATGTCATCAGGAGCGGCGAGGTAGGAGCTGTCTGCGGAACTGAGGAATCCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat              <  4:302431/151‑1 (MQ=255)
                                                                                                                                     gCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat              >  3:1866253/1‑150 (MQ=255)
                                                                                                                                       tAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaataat           >  7:734483/1‑151 (MQ=255)
                                                                                                                                       tAGGGGAAACGTGGATGTCATCAGGACAGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTTCAATATCTCCAGTACGACATCACCAAAGATATCTTCGCAACTCTTTGCGTGCTGCTTCAGGATGGAAAAaataat           >  2:1907089/1‑151 (MQ=255)
                                                                                                                                       tAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaataat           >  5:2095255/1‑151 (MQ=255)
                                                                                                                                        aGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATg          <  4:1701695/151‑1 (MQ=255)
                                                                                                                                                cGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCtt  <  4:929572/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547669‑4547963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑