Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,5970GC22.4% 377.7 / 41.4 268intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (77/89);  new base C (0/48);  total (86/182)
Fisher's exact test for biased strand distribution p-value = 1.62e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

GCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACA  >  DH1/613447‑613734
                                                                                                                                                           |                                                                                                                                          
gCCCGTACTGAACCCGGGTAGGCTGCTTGAGCGAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAg                                                                                                                                            <  2:332821/151‑1 (MQ=255)
 ccggTACTGAAGCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGTATGACTGTCCACGACAGAAGCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                                                                                                                           <  2:535657/148‑1 (MQ=255)
  ccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                          <  6:197201/151‑1 (MQ=255)
  ccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                          <  1:1047331/151‑1 (MQ=255)
   cGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa                                                                                                                                         <  1:621003/151‑1 (MQ=255)
      aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑AG‑CTTCGGCGGGTTTTTCCTTGTGGAGGGGCAGCAat‑at                                                                                                                                     >  4:720891/1‑151 (MQ=255)
      aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCAATATCATTCAAATTCACAAGATTTACG‑TAAAA‑A‑AC‑AT‑ATTATTAGTGTTTTTCATTTTGGAGGGGCAGaa                                                                                                                                          >  2:1153433/1‑147 (MQ=255)
      aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCGGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ac                                                                                                                                     <  3:1238920/151‑2 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaa                                                                                                                                  <  2:1421718/151‑1 (MQ=255)
         gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atga                                                                                                                                   <  8:1686402/150‑1 (MQ=255)
          acgcgggttattgttctTGAGCGAGTCATAGCTTGCTGGACTAGAGGACAGACTCTACACCAAAGAACCCGGCTTATCGGTCAGTTTCACCTGTTTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAG‑atgaa                                                                                                                                  <  3:206845/135‑1 (MQ=2)
          aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGGTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaat                                                                                                                                 <  3:1751409/151‑1 (MQ=255)
          aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaat                                                                                                                                 >  4:1237655/1‑151 (MQ=255)
          aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CA‑CG‑CTACGGCGGCTTTTTGCTTGTGGCGGGGCAGAAAG‑atgaat                                                                                                                                 >  7:413107/1‑151 (MQ=255)
           aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCTCCTGATTTAAG‑TAAAA‑A‑CC‑CC‑CATGCGCGGGTTTTTGCTTATGTAGGGGAAGAAAG‑atgaatc                                                                                                                                >  5:1220287/1‑150 (MQ=255)
           aCCCGGGTAGGCGGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaatg                                                                                                                                <  2:1806757/151‑1 (MQ=255)
             ccGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGAc                                                                                                                              <  1:792073/151‑1 (MQ=255)
             ccGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGATAT‑ctgaatacc                                                                                                                              >  8:811394/1‑148 (MQ=255)
               gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTg                                                                                                                            >  1:232491/1‑151 (MQ=255)
                ggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGt                                                                                                                           <  5:886680/151‑1 (MQ=255)
                 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTc                                                                                                                          <  1:413257/151‑1 (MQ=255)
                   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGTATGACTGTCCa                                                                                                                        <  1:1781826/151‑1 (MQ=255)
                   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTcc                                                                                                                         <  4:628875/150‑1 (MQ=255)
                    tgcggctTGAGGCCGGGACCGATTGCTGGACTTGACTACTCACTCTCCCCCACAGAACGCGGCTTATCGGTCAGTTTCACCTGTTTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCa                                                                                                                        <  5:1310627/146‑1 (MQ=11)
                     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGACTGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacg                                                                                                                      <  4:31680/151‑1 (MQ=255)
                     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacg                                                                                                                      <  4:1383399/151‑1 (MQ=255)
                     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacg                                                                                                                      <  5:1513756/151‑1 (MQ=255)
                           tGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGc                                                                                                                  <  8:161212/149‑1 (MQ=255)
                              gCCAGTGAGCGTTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCGGGCTTATGGGTCTGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCtata                                                                                                              <  8:1129072/150‑1 (MQ=255)
                                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAACCCGAAAATCGGTCAGTTTCAACTCATTTACG‑TAAAA‑A‑CA‑AG‑CTTCTGAAGCTTTTTGATTTTGGAGGGGCAGAAAG‑ATGAATGAAAGTCAACGACACtataac                                                                                                            >  8:1354305/1‑148 (MQ=18)
                                   tGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACAGATAATAAGACAGTATAAAATGATATAAG‑TAAAA‑A‑CA‑AT‑ATTCTAAAGCTTTTTGCTATTCGAATGAAAAAAAA‑ATTAAAGACTGTCCACGACACTATAcagat                                                                                                         >  6:594404/1‑146 (MQ=255)
                                      gCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCGATTATCATTCAGTTTCACATGATTTACG‑TAAAA‑A‑AC‑AT‑ATTATTAGGGTTTTTCATTTTGGAGGGGCAGAAAG‑ATGAATAACTGTACACGACACTATACCCaaaaa                                                                                                      >  8:339525/1‑149 (MQ=12)
                                       cGATTGCTGGCCTAGATGATTCACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGTTTTACT‑TAAAA‑A‑CC‑GT‑CTTCGGCGTGTTTTTGTTTTTGTAGGGGCATAAAG‑ATGAATTAGTGTCCACGACGCTATACCCAaaagaa                                                                                                    <  2:613131/151‑1 (MQ=18)
                                         tttgttgGCCTAGATTAATGACTGTCCACGACATAACCCGGATTATCGTTCATTTTCACGTCATTTACT‑TAAAA‑A‑CC‑CT‑CTTCGGCGGGTTTTTGTTTTTGGTGGGGCACAAAC‑ATGAATGACTGTCCACGACGCTATACCCAaaagaaa                                                                                                   <  2:1492125/149‑1 (MQ=255)
                                         aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCGGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGGTTTTGCTTTTGGAGGTGCAGAAAG‑ATGCTTGCCTGTCCACGACGCtatgcgggaaagctcg                                                                                                  >  7:859733/1‑138 (MQ=18)
                                          ttGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGAATCTCCACGCCGCTATCCCCAaaagaa                                                                                                    >  3:1560647/1‑148 (MQ=255)
                                           tGCTGGCCTAGATGAATGACTGTCCACGACAGAATACCGCATATCGCTCGGAGTCCGCTGAAGTACC‑TAAAA‑A‑AA‑AG‑AGTCGGCCGGTTTTTGCTTTGTGAGTGGAAGAAAG‑ATGACTGCGTGGCGCCGCCGCTCTAcccgagcggctggg                                                                                                >  1:1632194/1‑140 (MQ=255)
                                            ggtGGCGTAGTTGAATGACTGTCCACGCAAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGCATAAAG‑ATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCg                                                                                                <  3:979464/148‑1 (MQ=17)
                                             cTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGCCTTATCGGTCAGTTTCGCCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGCCGCTATACCCAAAAGAAAGCgcg                                                                                              >  3:1437461/1‑149 (MQ=255)
                                              tGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCt                                                                                             >  3:480970/1‑151 (MQ=255)
                                               ggCCTAGTTGAATGACTGTCCACGACAGAACCCGTCTTATCGGTAAGTTTCACCTGATTTACT‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                             <  6:760491/150‑1 (MQ=255)
                                               ggCCTAGATGAATGAGTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGGTTTTGGTGGGGCAGAAAG‑ATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGc                                                                                              <  8:1257756/149‑1 (MQ=255)
                                               ggCCTAGATGAATCCCTGGCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACG‑TAAAA‑C‑CC‑CG‑CTTCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATCAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                            <  5:528617/151‑1 (MQ=17)
                                                 ccTAGATGAATGACTGGCCACGACAGAACCGGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                          <  1:170795/151‑1 (MQ=255)
                                                    agttgaatgaCTGTCCACGACAGAACCCGGCTTATCGGTCTGTTTAACCTTATTTACG‑TAAAA‑A‑CC‑CG‑CTTGGGCGGGTTTTTGTTTTTGGAGGGGCAGAAAG‑ATGACTGACTGTCCACGACGCTATACCCAAACGAAAGCGGCTTATCg                                                                                        <  6:1308204/147‑1 (MQ=17)
                                                    aGATTAATGACTGGCCACCACAGAACCCGGCTTCTCGGTCAGTTGCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTGGGGGGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                        <  1:1554521/150‑1 (MQ=17)
                                                      atgaatgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaat                                                                                                                                 >  3:1233425/1‑107 (MQ=255)
                                                      atgaatgaCGGTCCACGACACAACCCGGCTTAGCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGTTTTTTGCTTTTGGAGGGGCATAAAG‑ATCAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                     <  7:1386863/151‑1 (MQ=17)
                                                        gaatgcCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                    <  2:493758/150‑1 (MQ=255)
                                                        gaatgaCTTTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                    <  4:1769083/150‑1 (MQ=255)
                                                            caCTGACCTCGACAGATCCCGGCTTATGGGTCCGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CC‑CTTCGGGGGGTTTTTGCTTTTGGCGGGGCACACAG‑ATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                <  3:1650055/149‑1 (MQ=14)
                                                             aCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACT‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAT‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                              <  7:1907951/151‑1 (MQ=255)
                                                                 cccgccaCAGAACCCGGCTTATCGGTCAGTTTCACGTGATTTAC‑‑TAAAA‑A‑CCGCG‑CTTCGGCGGGTTTTTGCTTTTGTCGGGGCTGAACG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                          <  3:475370/145‑1 (MQ=14)
                                                                   caccacAGAACCCGGCTTATCGGGCATTTTCCCCTGATTTCCT‑TAAAC‑C‑CC‑CG‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCACAAAG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                        <  7:1679053/151‑1 (MQ=14)
                                                                       aCAGAACCCGGCTTATCTGTAATTTTCACTTGA‑TTACGTTAAAC‑A‑CG‑GC‑CTGACGCGGTTTTTTGTTTTTGGCGGCGATCAAAT‑TTGATTGATTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                    <  6:1657958/151‑1 (MQ=9)
                                                                         acaccccGGCTAAGCGGGCAGTGCCACGTCA‑TTACC‑TAAAA‑ACCC‑CG‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCAAAACC‑ATGAAGGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGt                                                                  <  7:1027913/147‑1 (MQ=2)
                                                                          caaCCCGGCTTATCGGTCATTTTCCCCTTTTTTAGT‑TAAAA‑A‑GC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCTGAAAG‑TTGAATTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                 <  4:1336489/150‑1 (MQ=11)
                                                                          caaCCCGGCTAATCGGGAAGTTTCACCTGATTACCG‑AAAAA‑A‑CC‑CG‑CTGCGGCGGGTTTTTGATTTTGCAGGGGCACAATC‑ATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                 <  3:285400/150‑1 (MQ=14)
                                                                            aCCCGGATTATGGGTAATTTTCCGTTCATTTTGT‑TAAAA‑A‑CG‑GT‑CTTCTGCGGGTTTTTGTTTTTGTTGGTGCTCAATT‑TTGATTGACTTTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                               <  8:1000437/151‑1 (MQ=2)
                                                                             cccGGATTATGGGCAAGTTGCCGCTCATTTCCG‑AAAAA‑C‑CC‑CG‑CTGCGGCGGGTTTTTGTTTTTGGCGGGGCCAAATT‑TTGATTGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                              <  7:1136084/151‑1 (MQ=2)
                                                                                 gCTTATCGGTAAGTTTCACGTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGCAGGGGCCCAAAT‑ATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAccc                                                          <  7:34235/151‑1 (MQ=11)
                                                                                     ttgtgaaattttCACGTCATTTACG‑TAAAA‑A‑CC‑CT‑CTTCGCGGGGTTTTTGCTTTTGCTGGCGTAAAATT‑ATGAATGAATTTCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTTGTTTACGTAAAAACCCGCt                                                       <  8:888401/141‑1 (MQ=11)
                                                                                     atggttaattttCTCCTGATTTCGT‑TATAC‑C‑CC‑CC‑CTTCTGCGGGTTTTTGCTTTTGCCGGTGCTCAAAT‑ATGATTGACTGTACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                       <  6:1865837/141‑1 (MQ=2)
                                                                                     aTCGGTCAGTTTCACGTCATTTACG‑TAACA‑C‑CC‑GG‑CTTCGGCGGGTTTTTGGTTTTGTTGGGGCCCAATC‑ATGAATGAGTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt                                                      <  3:365511/151‑1 (MQ=11)
                                                                                       ggtgaaGTTTCACGTGATTTACT‑TAAAA‑A‑GC‑CT‑CTTCTGCGGGTTTTTGTTTTTGTTGGGGCATAAAT‑ATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                    <  4:157936/146‑1 (MQ=11)
                                                                                       cGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGATAG‑ATGAAAGACTGTCCACGACGCTATCACCAAAAGAAAGCGGCTGATCGGTCAGTTTAAACTGTTTTAGGTAAAAACCCgttccg                                                    >  7:1466969/1‑146 (MQ=11)
                                                                                        ggtcttttccatattaTTAACG‑TAAAA‑T‑CC‑CT‑CTTGGGCGGTTTTTTGATTTTGTTGGTGTTTTAGT‑TTGATTGACTGTCCTCGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                   <  4:351909/136‑1 (MQ=2)
                                                                                        gggcattttCCCTTGATTTCGG‑TAAAA‑A‑CC‑GG‑CTTCGGCGGGTTTTTGTTTTTGTTGGGGCAGAAAC‑ATGATTGACTGTACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                   <  4:84196/145‑1 (MQ=11)
                                                                                         cccAGTTGCACCTGA‑TTACC‑TAAAACC‑CC‑CG‑CTGCCGCGGGTTTTTGCTTTTGGCGGGGCACAACG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                  <  5:995707/149‑1 (MQ=11)
                                                                                            aGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGAGGCTTATCGGTCAGTTTGCCCTGGTGTACGTAATAACCCggtgcgcgggg                                               >  1:935347/1‑141 (MQ=11)
                                                                                              ttggcgcTGATTTTCG‑TAACA‑C‑CG‑GT‑CTGCGGCGGGTTTTTGGTTTTGTTGGGGCTTACTT‑TTGAATGACTTTCCACGACGCTTTACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGt                                              <  4:1705907/144‑1 (MQ=2)
                                                                                               ttCACGTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGGTTTTGTAGGCGCAGAAAG‑ATCAATGACTGTCCACGACGCTATACCCAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                             <  7:1494031/150‑1 (MQ=11)
                                                                                                 cccatGATTTACT‑AAAAC‑C‑CC‑CC‑CTGCGGCGGGTTTTGGGTTTGGGCGGGCCCCACCC‑ATCAATCCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                          <  7:760649/147‑1 (MQ=2)
                                                                                                 caccgcaattACG‑TAAAC‑C‑CC‑GG‑CTGAGCCGGGTTTTTGCTTTTGGCGGGGCCCGCAG‑ATGACGCCGTGGCCACGACGCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                          <  5:1116181/143‑1 (MQ=2)
                                                                                                  cccTGATTACCG‑AAAAC‑C‑CC‑CG‑CTGCGGCGGGTTTTTGCTGTTGCCGGCGCACAACC‑ATGAAGGACTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTg                                         <  5:376237/150‑1 (MQ=11)
                                                                                                      gATTTCCG‑GAAAC‑C‑CC‑GG‑CTGCGGCGGGTTTTTGCTTTTGCAGGGGCAGAACG‑ATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                     <  5:569068/151‑1 (MQ=11)
                                                                                                      gATTTACG‑TAAAA‑A‑CG‑CT‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGCAGTATG‑ATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtt                                      <  6:1106784/150‑1 (MQ=11)
                                                                                                      caTTTACG‑AAAAA‑A‑CC‑CG‑CTTCCGCGGGTTTTTGCTTTTGCAGGGGCTCAATC‑ATGAATGCCTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                       <  3:89685/148‑1 (MQ=11)
                                                                                                          tttcggtaaa‑a‑CC‑CGTCTTCTGCGGGTTTTTGGTTTTGGAGGGGCCTAATT‑TTGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                    <  4:410692/143‑1 (MQ=11)
                                                                                                          tACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGATAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATAACCCGGTGCGGCGGGGGTGTGCTTGTggg                                 >  1:1081888/1‑150 (MQ=11)
                                                                                                           ataacgttcaaaccgcG‑CTGCTGCGGGTTTTTGTTTTTGTTGGCGTTTAATT‑ATGAAGGACTCGCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                    <  6:1375259/136‑1 (MQ=2)
                                                                                                           agg‑TAAAA‑A‑CG‑GT‑CTTCGGCGGTTTTTTGGTTTTGTTGGTGCTCATAG‑ATGATTGACTGTCCACGACGCTTTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                                 <  8:624977/148‑1 (MQ=11)
                                                                                                           aCG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTGCGGCGGGtttgagcggttgggg                                >  1:601230/1‑139 (MQ=11)
                                                                                                            cG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACCCTAGACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTGAGCTTGTGGAgg                               >  1:18509/1‑151 (MQ=11)
                                                                                                             g‑TAAAA‑C‑CC‑CG‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAAAC‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                              <  3:1461724/151‑1 (MQ=11)
                                                                                                                aaaa‑a‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAACCCGGTGCGGCGGGTTTTTGATTGTGGAgggcg                            >  3:735815/1‑149 (MQ=11)
                                                                                                                 tcaacccc‑cc‑CTGCGGCGGGTTTTGGGTTTTGCCGGCGCCAAACC‑AGGAATGCCTGCCCCCGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                               <  7:604093/143‑1 (MQ=11)
                                                                                                                 gttacacccgctcTTAGGGGGGTTTTTGGTTTTGTTGGGGTATAATG‑ATGATTGACTGTCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                               <  8:1524429/139‑1 (MQ=11)
                                                                                                                  aa‑a‑CC‑GG‑CTTCGGCGGGTTTTTGCTTTTGGCGGTGCCCAACC‑ATGCAGGACTCTCCACGACGCTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAg                          <  1:719397/151‑1 (MQ=11)
                                                                                                                  aa‑a‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAAACCGTTACGGCGGGTTTGAGCTTGTGGAgggcgcg                          >  5:226131/1‑147 (MQ=11)
                                                                                                                        c‑cG‑CTGCGGCGGGTTATTGCGTTTGCCGGCGGCCCACG‑ATGAATGCCTCGCCACGACGCTATACCCAACAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                      <  1:1374711/151‑1 (MQ=11)
                                                                                                                           g‑CTGCGGCGGGTTTTTGGTGTTGTCGGCGC‑GAAAGTATGATGGCCTGGCCCAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                    <  5:1751652/151‑1 (MQ=11)
                                                                                                                             cTTCGGCGGGTTTTTGCTTTTGGAGGGGCAAAATG‑CTGAGTGACGGGCGAGGACCGGATAACCAAAAGAAAGCGGGTTATCGGTCAGTTTGGCGTGGTTTACGTAAAAACCCGCTTCGGCGGGTGTTTGCTTTTGGAGGGGCAggaggggg                   >  7:502160/1‑144 (MQ=2)
                                                                                                                               gcggcggGTTTTTGGTTTTGGTGGTGAATCAGT‑TTGATGGCCTGGCCACGAGGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa                 <  7:1615929/150‑1 (MQ=11)
                                                                                                                                aggcggGTTTTTGTTTTTGTTGGCGCTTAATT‑ATGATTGACTTGCCTAGACGCTATACACTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                <  8:186361/150‑1 (MQ=11)
                                                                                                                                 ggcggTTTTTTGTTTTTGTCGCAGCCAAAAA‑ATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg               <  1:868671/151‑1 (MQ=11)
                                                                                                                                 cgcggGTTTTTGGTTTTGCCGGGGCATAACC‑ATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg               <  7:516919/150‑1 (MQ=11)
                                                                                                                                   cggTTTTTTGTTTTTGGAGGTGCATAACT‑ATGATTGACTGTCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAc             <  7:832631/151‑1 (MQ=11)
                                                                                                                                     ggTTTTTGCTTTTGGCGGGGCTCAACT‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg           <  7:1314200/151‑1 (MQ=11)
                                                                                                                                         tttGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCt                                                                           >  7:1363119/1‑83 (MQ=18)
                                                                                                                                                           |                                                                                                                                          
GCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAA‑A‑CC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACA  >  DH1/613447‑613734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑