Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6130TG25.7% 219.0 / 38.7 219intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (58/101);  new base G (6/49);  total (66/153)
Fisher's exact test for biased strand distribution p-value = 2.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCG  >  DH1/613463‑613761
                                                                                                                                                           |                                                                                                                                                     
 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTc                                                                                                                                                      <  5:861966/151‑1 (MQ=255)
 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTc                                                                                                                                                      <  1:413257/151‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGACGGCGTATAGGGCACAATGCGGTGATGTACG‑TAAAATAC‑A‑C‑CATCTGCTGGGTTTTGGTGTTG‑GTGGTCCAGAAAGATGCATGCCTGGccg                                                                                                                                                    >  1:1503871/1‑150 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGTATGACTGTCCa                                                                                                                                                    <  1:1781826/151‑1 (MQ=255)
     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGCCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                                  <  8:1212338/151‑1 (MQ=255)
     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                                  <  4:1383399/151‑1 (MQ=255)
     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                                  <  5:1513756/151‑1 (MQ=255)
      ctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGTCAGAACCCGGCTTATCGGTCAGTTTCACCTGATATACG‑TAAATACC‑C‑C‑CTGCGACGTCGATTGGCATTTG‑GAGGGGCAGTATGCTGAATGCATGTCCa                                                                                                                                                    >  1:489497/1‑148 (MQ=25)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                                <  7:231293/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                                <  8:1105221/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTAAG‑TAAAAAAC‑A‑G‑CTTCGTCTAGTTATTGATTTTG‑TAGAGTAATAAAGATGAATGACTGTCCacgaa                                                                                                                                                >  6:1082878/1‑149 (MQ=25)
           tGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGc                                                                                                                                              <  5:1100390/149‑1 (MQ=255)
           tGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGGTGTTGCTGTTT‑GGGGGGCAGAAAGATGAATGACTGTGCacgcctctg                                                                                                                                            >  3:351243/1‑145 (MQ=21)
             aGCCAGTTAGCGATTGGTGGCGTAGTTGAATGACTGTCCACGACAGAACCCGGCTTATGGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑GTTCGGGGGGTTTTTGCTTTTT‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCtata                                                                                                                                          <  3:439312/151‑1 (MQ=18)
             aGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCtat                                                                                                                                           >  5:1673149/1‑150 (MQ=255)
                 aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCATTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCa                                                                                                                                      <  7:1537684/151‑1 (MQ=255)
                   tGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACAGATAATAAGACAGTATAAAATGATATAAG‑TAAAAACA‑A‑T‑ATTCTAAAGCTTTTTGCTATTC‑GAATGAAAAAAAAATTAAAGACTGTCCACGACACTATAcagat                                                                                                                                     >  6:594404/1‑146 (MQ=255)
                      gCGATTGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GCGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaa‑aga                                                                                                                                <  5:1168032/151‑1 (MQ=255)
                       ccaTTGCTGGCCTATCTTAATGACTGCCCACCCCATACCCCGGCTTTTGGGTCAGTTTCACGTGATTTACT‑TAAAACCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTG‑TAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaa‑agaa                                                                                                                               <  8:1575558/149‑1 (MQ=14)
                       cGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGGCTTATCGGTCAGTTTCAGCTGATTTACG‑TAAAAACG‑C‑G‑CGTCGGCGGGATTGTGGGTTTG‑GATTGGCAGAAAGATGAATGACTGTCGACGGCACTCTAcccgctcggg                                                                                                                                >  3:119571/1‑144 (MQ=18)
                        gATTGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATCAATGACTGGCCACGACGCTATACCCAaa‑agaaa                                                                                                                              <  5:131922/151‑1 (MQ=255)
                         aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCGGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGGTTTTGCTTTTG‑GAGGTGCAGAAAGATGCTTGCCTGTCCACGACGCtatgcgggaaagctcg                                                                                                                              >  7:859733/1‑138 (MQ=18)
                          ttgggggCTTAGATCATTGAGTGCCCACGACACAACCCGGCTTACCGTTCAGTTTCACGTTATTTAGT‑TAAAAACC‑C‑T‑CTTGGGCGGGTTTTTGCTTTTG‑TCGGGGAACAAATATCATTTACTGTCCACGACGCTATACCCAaa‑agaaag                                                                                                                             <  8:1760588/145‑1 (MQ=255)
                            ggtGGCGTAGTTGAATGACTGTCCACGCAAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑TAGGGGCATAAAGATGATTGACTGTCCACGACGCTATACCCAAA‑AGAAAGCg                                                                                                                           <  3:979464/148‑1 (MQ=17)
                             cTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGCCTTATCGGTCAGTTTCGCCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGCCGCTATACCCAAA‑AGAAAGCgcg                                                                                                                         >  3:1437461/1‑149 (MQ=255)
                             cTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACC‑TAAAAACC‑C‑C‑CATCGGC‑GGCTGTGGCGTCTGCGAGCGCCAGAAAGAAGGAGGACTGGCGCGGCCACTATACCCCAA‑AGCAAGCggg                                                                                                                         >  1:763062/1‑150 (MQ=12)
                               ggCCTAGTTGAATGACTGTCCACGACAGAACCCGTCTTATCGGTAAGTTTCACCTGATTTACT‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCt                                                                                                                        <  6:760491/150‑1 (MQ=255)
                               ggCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACG‑TAAAAAAC‑C‑G‑ATTATCCGAAGATATTCGTGTA‑GAGCAACAGAATGATGAATAAATTTCAACTACGATATACCCAaa‑agaaataagaa                                                                                                                        >  6:1340529/1‑144 (MQ=255)
                                gCCTAGATCAATGACTGGCCACCACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTCCG‑TAAAACCC‑C‑G‑CTTCGGGGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCttn                                                                                                                      <  8:142676/151‑2 (MQ=17)
                                 ccTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCACAAAGATGAATCACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTAt                                                                                                                     <  5:446820/151‑1 (MQ=255)
                                 ccTAGATGAATGACTGTCCACGACAGAAAACGGATAATCAGTCACTATCACAAGATTTACG‑TAAAAACA‑A‑A‑CTTCGGATGGTTTTTGCTTTTG‑GAGGGGCAGAAACATGAATGACTTTCAAAGACGCTATACCCAAA‑AGAAAGCGGCTTAt                                                                                                                     >  4:795862/1‑151 (MQ=17)
                                   ccgacctatccctttCCACCAAAGAACGCGGCTTTTCGTTTTTTTTCACTTTTTTTACT‑TAAAAACC‑C‑G‑CTTCGTCGGTTTTTTTCTTTTG‑GAGGGGCATAAAGATCAATGACTGTCCACGACACTATACCCAAA‑AGAAAGCGGCTTATCg                                                                                                                   <  2:1715805/137‑1 (MQ=2)
                                    agttgaatgaCTGTCCACGACAGAACCCGGCTTATCGGTCTGTTTAACCTTATTTACG‑TAAAAACC‑C‑G‑CTTGGGCGGGTTTTTGTTTTTG‑GAGGGGCAGAAAGATGACTGACTGTCCACGACGCTATACCCAAA‑CGAAAGCGGCTTATCg                                                                                                                   <  6:1308204/147‑1 (MQ=17)
                                     gATGAATGACTGTCCACGACAGAACCCGCCTTATCGGTCAGTTTCACATGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGATTTTG‑GAGGGGCAGAAAGATGAATGACTGTACACGACACTATACCCAAA‑AGAAAGCGGCTTAtatcg                                                                                                                 >  2:1420466/1‑147 (MQ=17)
                                        gaatgcCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCa                                                                                                               <  2:493758/150‑1 (MQ=255)
                                        gaatgaGTGTCCACTACTAAACCCTGCTTATGGGTCATTTTCACGTGATTTTCG‑TAAAAACG‑C‑T‑CTGGGGCGGGTTTTTGCTTTTG‑GAGGGGCTCAAAGATGAATGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCa                                                                                                               <  5:987348/150‑1 (MQ=14)
                                          atgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACGTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGGGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATCCCCAAA‑AGAACGCGGCTTATCgggctcgg                                                                                                            >  1:1713885/1‑145 (MQ=14)
                                            caCTGACCTCGACAGATCCCGGCTTATGGGTCCGTTTCACCTGATTTACG‑TAAAAACC‑C‑C‑CTTCGGGGGGTTTTTGCTTTTG‑GCGGGGCACACAGATGATTGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGttt                                                                                                           <  3:1650055/149‑1 (MQ=14)
                                             agttccctctaCATAACCCGGCTTATGGTTCATTTTCACTTGATTTACT‑TAATATCG‑C‑T‑TTTCGGGGGTTTTTTGTTTTTT‑TTTGTTTTTAATTTTTATTTACTTTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCa                                                                                                         <  4:1764054/141‑1 (MQ=2)
                                             actttcacagACAGAACCCCGCTTATGCGTTAGTTTACCCTGATTTACT‑TAAAAACC‑T‑T‑CTTCTGCGGTTTTTTGTTTTTG‑TTGGTGCTTAAATATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCa                                                                                                         <  6:1879697/142‑1 (MQ=9)
                                             aatttcctctatataaCCCGGTTTATCTGTAATTTTAACATTATTTACT‑TAAAAACC‑C‑G‑CTTCGGGGTTTTTTTGTTTTTG‑TTGGGGTTTAATTTTGAATGACTTTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTc                                                                                                          <  8:1281936/136‑1 (MQ=2)
                                                ggcccccaCAGAACCCGGCTTATGGGTCAGTTTCACCTGATTTACT‑TAAAACCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑TCGGGGCAGAAAGATGATTGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                                      <  1:215357/144‑1 (MQ=14)
                                                gTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGc                                                                                                                         >  1:927075/1‑132 (MQ=255)
                                                ccccACAACACAACCCGGATTATGGGCAATTTTCCCCTCATTAACT‑AAAAACCC‑C‑T‑CTGCGGGGGTTTTTTGGTTTTG‑GAGGCGCATAAATATGATTGACTCTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                                      <  7:364825/149‑1 (MQ=9)
                                                 cccgccaCAGAACCCGGCTTATCGGTCAGTTTCACGTGATTTAC‑‑TAAAAACCGC‑G‑CTTCGGCGGGTTTTTGCTTTTG‑TCGGGGCTGAACGATGAATGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                                     <  3:475370/145‑1 (MQ=14)
                                                  ccACGACAGAACCCGGCTTATGGGGCAGTTTCACCTGATTAACG‑AAAAAACC‑C‑C‑CTGCGGCGGGTTTTTGCTTTTG‑GAGGGGCACAAACATGAATGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTgg                                                                                                    <  3:1564336/151‑1 (MQ=14)
                                                    cccaCAGAACCCGGCTTATGGGCCATTTTCACGTCATTTAGG‑TAAAAACG‑G‑G‑CTTCGGCGGGTTTTTGGTTTTG‑GTGGGGCCCAAATATGAATGACTTTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                                  <  4:1818959/148‑1 (MQ=14)
                                                    aCGACAGAACCCGGCTTATCGGGAAGTTTCACGTGATTTACG‑TAAAAACG‑C‑T‑CTGCGGCGGGTTTTTGCTTTTG‑CTGGGGCCTAAAGATGATTGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                                  <  4:32541/151‑1 (MQ=14)
                                                      gACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACT‑AAAAAAAC‑C‑G‑GGTGGGAGGGTTTTTGGTTTTG‑GATGAGGATAAATATGAATGAGGGTCCAATAATGTATAAAAAAA‑AGAAAGCGGCTTAGCGGTCGGtttggcggggggtg                                                                                                >  4:1158577/1‑140 (MQ=255)
                                                       aCAGAACCCGGCTTATCTGTAATTTTCACTTGA‑TTACGTTAAACACG‑G‑C‑CTGACGCGGTTTTTTGTTTTTG‑GCGGCGATCAAATTTGATTGATTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                                               <  6:1657958/151‑1 (MQ=9)
                                                          caaCCCGGTTTATGGGTCAGTATCACCTCATTTACG‑‑AAAAACC‑C‑GTCTTAGCGGGGTTTTTCCTTTTG‑GAGCGGCATAAACATGATGCACTGGCCACGAGGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                                            <  7:1371347/150‑1 (MQ=9)
                                                          caaCCCGGCTTATCGGTCATTTTCCCCTTTTTTAGT‑TAAAAAGC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCTGAAAGTTGAATTACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                                            <  4:1336489/150‑1 (MQ=11)
                                                          caaCCCGGCTAATCGGGAAGTTTCACCTGATTACCG‑AAAAAACC‑C‑G‑CTGCGGCGGGTTTTTGATTTTG‑CAGGGGCACAATCATGATTGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                                            <  3:285400/150‑1 (MQ=14)
                                                            aCCCGGCTTAACGGCAATTTGCCCGTGATTTACG‑AAAACCCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTG‑GCGGCGCCGAACGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                          <  1:16944/151‑1 (MQ=14)
                                                            aCCCGGATTATGGGTAATTTTCCGTTCATTTTGT‑TAAAAACG‑G‑T‑CTTCTGCGGGTTTTTGTTTTTG‑TTGGTGCTCAATTTTGATTGACTTTCCACGACTCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                          <  8:1000437/151‑1 (MQ=2)
                                                             cccGGATTATGGGCAAGTTGCCGCTCATTTCCG‑AAAAACCC‑C‑G‑CTGCGGCGGGTTTTTGTTTTTG‑GCGGGGCCAAATTTTGATTGACTCGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                                         <  7:1136084/151‑1 (MQ=2)
                                                                ggCTTATCGGGCACTTTCCGGTGATTACCT‑TAAAACCG‑G‑G‑CTGCGGCGGGTTTTTGTTTTTG‑CTGGGGCCCAAACTTGAATGACTTGCCACGACTCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAcc                                                                                      <  4:1689474/151‑1 (MQ=2)
                                                                    tATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGGTTCGGCAGTGGCCACGACTGAAGTCCAGAC‑ACATAGCTGCTTATCGGTTGGGTTCTGCTGGTTTAATTAAAAACCCGcg                                                                                  >  3:150962/1‑150 (MQ=2)
                                                                    tATCGGTAAGTTGCACATCATTTACG‑TAAAACCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GCGGGGCAGAAATATGAATGCCTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCg                                                                                    <  7:526677/149‑1 (MQ=11)
                                                                    tATCGGGAATTTGCACGTGATTTAC‑‑TAAAAACC‑CGG‑CTTCGGCGGGTTTTTGGTTTTG‑CCGGCGCTTAACGATGATTGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                                   <  1:1210438/150‑1 (MQ=11)
                                                                     ttgtgaaattttCACGTCATTTACG‑TAAAAACC‑C‑T‑CTTCGCGGGGTTTTTGCTTTTG‑CTGGCGTAAAATTATGAATGAATTTCCACGACGCTATACACAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTTGTTTACGTAAAAACCCGCt                                                                                  <  8:888401/141‑1 (MQ=11)
                                                                       ggtgaaGTTTCACGTGATTTACT‑TAAAAAGC‑C‑T‑CTTCTGCGGGTTTTTGTTTTTG‑TTGGGGCATAAATATGAATGACTTTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                               <  4:157936/146‑1 (MQ=11)
                                                                        ggtcttttccatattaTTAACG‑TAAAATCC‑C‑T‑CTTGGGCGGTTTTTTGATTTTG‑TTGGTGTTTTAGTTTGATTGACTGTCCTCGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                                              <  4:351909/136‑1 (MQ=2)
                                                                        gggcattttCCCTTGATTTCGG‑TAAAAACC‑G‑G‑CTTCGGCGGGTTTTTGTTTTTG‑TTGGGGCAGAAACATGATTGACTGTACACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                                              <  4:84196/145‑1 (MQ=11)
                                                                        ggTCAGTTGCACCTGATTTACG‑AAAAACCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTG‑GCGGGGCACAAAGATCAATGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTTCGTAAAAACCCGCTTcgg                                                                              <  7:1445285/151‑1 (MQ=11)
                                                                             atttgcccttgattACC‑TAACAACCGC‑G‑CTGGGGCGGTTTTTTGGTTTTG‑GCGGGGCACAAAGAGCAAGGCCTCGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCggg                                                                          <  7:436543/139‑1 (MQ=2)
                                                                               tgcACGTGATTTAGT‑TAACAACC‑C‑G‑GTTGGGCGGGTTTTTGGTTTTG‑GAGGGGCTCAATGTTCAATGACTGTCCTCGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                                                        <  8:204496/148‑1 (MQ=11)
                                                                                gccccTCATTTCCG‑TAAAACCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTG‑CCGGCGCCCAAACATGAAGGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtttt                                                                      <  3:535714/148‑1 (MQ=11)
                                                                                 cccatGATTTACT‑AAAACCCC‑C‑C‑CTGCGGCGGGTTTTGGGTTTGG‑GCGGGCCCCACCCATCAATCCCTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                                     <  7:760649/147‑1 (MQ=2)
                                                                                   ccTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GTGGGGCTGAAAGAGGACTGACGGTCCCGGACGCTAGACCCAAA‑AGAAAGCGGCGTTGCGGTCAGTTTCACCTGGTTTACGTAATAACCCGCGTCGGCGGGgttgggg                                                                   >  1:105600/1‑147 (MQ=11)
                                                                                   ccTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGAGTCTCGACGCCCCTATACCCAAA‑AGAACGCGGCGTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCggggtggtgg                                                                   >  7:1487219/1‑144 (MQ=11)
                                                                                    cctcattaccgaaaaacCC‑C‑G‑CTCGGGCGGTTTTTTGCTTTTG‑GAGGGCAACAAAGAAGAACGACTCGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGc                                                                   <  7:1025490/139‑1 (MQ=11)
                                                                                      gATTTCCG‑GAAACCCC‑G‑G‑CTGCGGCGGGTTTTTGCTTTTG‑CAGGGGCAGAACGATGAAGGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                                <  5:569068/151‑1 (MQ=11)
                                                                                      caTTTACG‑AAAAAACC‑C‑G‑CTTCCGCGGGTTTTTGCTTTTG‑CAGGGGCTCAATCATGAATGCCTCTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                                                  <  3:89685/148‑1 (MQ=11)
                                                                                       aTTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGCCTGTCCACGACGCTATACCCAAA‑AGAACGCGGCTGATCGGTCAGTGTGACCTGGTGTACGTAAAAACCCGGTCCGGCGGGGTTGTGAtttt                                                               >  1:958022/1‑151 (MQ=11)
                                                                                        tttACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTGCGGCGGGTTTGTGCttttt                                                              >  5:362847/1‑150 (MQ=11)
                                                                                          tttcggtaaaaCC‑C‑GTCTTCTGCGGGTTTTTGGTTTTG‑GAGGGGCCTAATTTTGATTGACTTTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                               <  4:410692/143‑1 (MQ=11)
                                                                                           agg‑TAAAAACG‑G‑T‑CTTCGGCGGTTTTTTGGTTTTG‑TTGGTGCTCATAGATGATTGACTGTCCACGACGCTTTACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                                                            <  8:624977/148‑1 (MQ=11)
                                                                                           aCG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAATAACCCGGTGCGGCGGGTTTGTGCTTGTgg                                                             >  5:182021/1‑149 (MQ=11)
                                                                                           aCG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTGCGGCGGGtttgagcggttgggg                                                           >  1:601230/1‑139 (MQ=11)
                                                                                            cG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACCCTAGACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTGAGCTTGTGGAgg                                                          >  1:18509/1‑151 (MQ=11)
                                                                                             cgaaaccccc‑c‑c‑CTGCGGGGGGTTTTTGGTTTTG‑CGGGTGCACAACCATGATGGCCTTGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                                          <  1:626034/143‑1 (MQ=11)
                                                                                             acgaaacaCC‑CGG‑CTGCGCCGGGTTTTTGGTTTTG‑GCGGCGCCCCACCATGATTGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAg                                                           <  1:1603695/148‑1 (MQ=11)
                                                                                               tcaacccc‑c‑c‑CTGCGGCGGGTTTTGGGTTTTG‑CCGGCGCCAAACCAGGAATGCCTGCCCCCGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                                          <  7:604093/143‑1 (MQ=11)
                                                                                                gttacacccgctcTTAGGGGGGTTTTTGGTTTTG‑TTGGGGTATAATGATGATTGACTGTCCACGACGCTATACACAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                                          <  8:1524429/139‑1 (MQ=11)
                                                                                                aaaaaCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAACCCGGTGCGGCGGGTTTTTGATTGTGGAgggcg                                                       >  3:735815/1‑149 (MQ=11)
                                                                                                   aaCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAAAATAGTAACAGACAACTACACTAAAACAAAA‑ATAAAAATACTTATCGTAAAGTTTGACCTCGTTTAAGTAAAAAAAAGCTTCGGCGGGTTTTTGCTTTTGGAGGGGcggg                                                    >  8:829360/1‑148 (MQ=2)
                                                                                                      c‑c‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTTACCTGCTTTACGTAAAAACCCGTTTCGGCGGGTTTAAGATTTTGGAGTGGcctttat                                                 >  7:1652080/1‑145 (MQ=11)
                                                                                                      c‑c‑G‑CTGCGGCGGGTTATTGCGTTTG‑CCGGCGGCCCACGATGAATGCCTCGCCACGACGCTATACCCAAC‑AGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                                                 <  1:1374711/151‑1 (MQ=11)
                                                                                                            cTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAAAATGCTGAGTGACGGGCGAGGACCGGATAACCAAA‑AGAAAGCGGGTTATCGGTCAGTTTGGCGTGGTTTACGTAAAAACCCGCTTCGGCGGGTGTTTGCTTTTGGAGGGGCAggaggggg                                              >  7:502160/1‑144 (MQ=2)
                                                                                                              tCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATCAAAAAATCTACAAGAAGCTAAACACAAT‑TAAAATAAGTTTATATCTCAGTTTAACCAGTATAAATAAAAAAAAAAATTCGGCGGGTTTTTGGGGTTGGAGGGGGAGAAAGatggg                                            >  6:1577643/1‑149 (MQ=255)
                                                                                                              gcggcggGTTTTTGGTTTTG‑GTGGTGAATCAGTTTGATGGCCTGGCCACGAGGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa                                            <  7:1615929/150‑1 (MQ=11)
                                                                                                              gcgccggGTTTTTGCTTTTG‑GAGCGGCACAACCATGAATGCCTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa                                            <  7:1739225/147‑1 (MQ=11)
                                                                                                                ggcggTTTTTTGTTTTTG‑TCGCAGCCAAAAAATGAAGGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                          <  1:868671/151‑1 (MQ=11)
                                                                                                                  cggTTTTTTGTTTTTG‑GAGGTGCATAACTATGATTGACTGTCCACGACGCTATACACAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAc                                        <  7:832631/151‑1 (MQ=11)
                                                                                                                    ggTTTTTGCTTTTG‑GCGGGGCTCAACTATGAATGACTGGCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                                      <  7:1314200/151‑1 (MQ=11)
                                                                                                                        tttGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTTACCt                                                                                                      >  7:1363119/1‑83 (MQ=18)
                                                                                                                            cTTTTG‑GAGGGGCAGAAAGATGAATGAATATCCAAAACGCTAAAACAAAA‑AAAAAACTGCTTATCGTTCAGTATAACAAGGTTTACGTAAAAACAATAATAAAAAAGTTTTTTCTTTTGGAGGGGCAGAAAGatgaatgaagatcggctgc                              >  2:506623/1‑140 (MQ=2)
                                                                                                                                                        cTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACAAAACAAAaaagaaatggaattaa    >  8:395172/1‑141 (MQ=11)
                                                                                                                                                           tCCACGCCGCTTTTCCGAAATAGAACGCGGCTTATGGGTCTTTTTCACCTTTTTTACTTAAAAACCGGTTTGGGGGGTTTTTTTTTTTTGGAGGGGAATAAATATTATTGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCg  <  2:1532277/150‑1 (MQ=0)
                                                                                                                                                           |                                                                                                                                                     
GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACG‑TAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAA‑AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCG  >  DH1/613463‑613761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑