Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA31.6% 265.7 / 43.7 214intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (103/18);  new base A (55/1);  total (195/19)
Fisher's exact test for biased strand distribution p-value = 7.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

CTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613622‑613919
                                                                                                                                                     |                                                                                                                                                     
  aagccggaaaaagcgCGGCTTATCGGTGAGTTTC‑CCGGTTAGGACTTAAAAACCGGGCTCGCCGGGTTTTTGGTTTTGCTGGCGGCCAACCTTGATTGCCTTGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                                                                                   <  1:403809/137‑1 (MQ=2)
        aacagaagccGGCTTATGGCTCAGTCTCCCCTTGT‑TAACGTACATCCCCGCTTCGGGGTGTTTTTGCTTTTGCTGGCGCTCAAATATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTa                                                                                                                                             <  5:1083777/142‑1 (MQ=2)
          aacaacgCGGCTTATCGGGCATTTGCAGCTGCT‑TTCCGAAAACCCCCCCTGCGGCGGGTTTTTGGTTTGGTCGGGGCACAAAGATGATTGCCTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACg                                                                                                                                           <  3:628645/145‑1 (MQ=2)
                     ttATCGGTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGCCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGc                                                                                                                                >  7:1778526/1‑151 (MQ=255)
                     ttATCGGTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCgg                                                                                                                                >  3:1674185/1‑150 (MQ=25)
                         cGGTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTAcg                                                                                                                            >  3:1065532/1‑151 (MQ=16)
                         cGGTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGCTCAGTTTAACCAGTTATACGTAAAAAACCGCTAcg                                                                                                                            >  2:37664/1‑151 (MQ=11)
                           gTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGCTAcggc                                                                                                                          >  3:1012349/1‑151 (MQ=16)
                            tCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGCAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAAACCGGTCcggcg                                                                                                                         >  3:5175/1‑151 (MQ=11)
                            tCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAAACCGCTAcggcg                                                                                                                         >  1:594990/1‑151 (MQ=255)
                            tCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTcggcg                                                                                                                         >  5:72815/1‑151 (MQ=25)
                            tCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGCTgcggcg                                                                                                                         >  5:575788/1‑151 (MQ=255)
                            tCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGCTTcggcg                                                                                                                         >  6:144495/1‑151 (MQ=25)
                             cAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTCcggcgg                                                                                                                        >  7:838688/1‑151 (MQ=25)
                                tttCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATTCGGCGGGtt                                                                                                                     >  8:74426/1‑151 (MQ=255)
                                 ttCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGACGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGCAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGATACGGCGGCttt                                                                                                                    >  6:288160/1‑151 (MQ=11)
                                  tCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAACCGATTCGGCGGGtttt                                                                                                                   >  6:781656/1‑151 (MQ=255)
                                   cACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGttta                                                                                                                   >  2:201738/1‑149 (MQ=11)
                                    aCCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGCCAGGTGTTACGTCTAAACCCGCGTCGGCggggtgttg                                                                                                                 >  7:1189053/1‑145 (MQ=11)
                                      cTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTTCGGCGGGtttgtcct                                                                                                               >  5:767631/1‑146 (MQ=255)
                                       tGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTATAAtt                                                                                                              >  8:763857/1‑151 (MQ=255)
                                       tGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTTTAACCTGTTTTACGTAAAAAACCGATGCGGCGGGtttatgatt                                                                                                              >  4:487432/1‑145 (MQ=11)
                                         gctgaaCGTAAACACCCGCTTCGTCGGGTTTTTGTTTTTGTAGGCTCAGAAACATCAATCCATGTACACTACTCTATACCCAATAGAAATCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACtta                                                                                                             <  2:105231/146‑2 (MQ=11)
                                         gT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTTGTGCttgt                                                                                                            >  3:1436772/1‑149 (MQ=11)
                                             tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTGAGCTTTTgg                                                                                                          >  5:1586447/1‑150 (MQ=255)
                                             tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATTCGGCGGGtttaagatttttg                                                                                                          >  4:1857322/1‑140 (MQ=11)
                                              aCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTGCTTGTgg                                                                                                          >  3:1572670/1‑149 (MQ=9)
                                              aCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGtttgtgatttttg                                                                                                          >  5:169012/1‑139 (MQ=255)
                                                 tAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCGGCGGCGGGGGTGAACTTGTGGAgggg                                                                                                     >  5:361547/1‑151 (MQ=11)
                                                  aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTATACTTTTGGAGTGGc                                                                                                    >  8:1514411/1‑151 (MQ=255)
                                                  aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTCCGGCGGGTTTATGCTTTTTGAgggcc                                                                                                    >  7:878331/1‑149 (MQ=11)
                                                      aCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAgtgcg                                                                                                    >  1:1379799/1‑143 (MQ=255)
                                                       cccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTTAAACTTTTTGAgtgccatat                                                                                                >  2:520162/1‑142 (MQ=11)
                                                             tCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTTCTTTTGGAGGGGCAGAAAGatgt                                                                                          >  7:112231/1‑149 (MQ=255)
                                                             tCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATCCGGCGGGTTTTAGATTTTTGAGGGCGAGaaatagaa                                                                                          >  1:1657307/1‑145 (MQ=11)
                                                                gcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTACGGCGGGTTTGAACTTTTTGAGTGGCAGATAGaagaatcc                                                                                      >  8:1350841/1‑149 (MQ=11)
                                                                gcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGACAACGGAACAAAAACCAAAAGAAAAAGACTTATAAGAAAATTTAAGATGTATTACGTAAAAAAAACATTAAAAAAAATTTTGCTTTTGGAGGGGCAGAAAGatgaatgc                                                                                      >  4:571899/1‑150 (MQ=2)
                                                                 cggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTTGAGTGCCAGAAATacgaaaccc                                                                                     >  4:1441309/1‑147 (MQ=11)
                                                                 cggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTAGACCTGTTTTACGTAATAAACCGCTGCGGCGGGTTTTTACTTTTGGAGGGGGAGTTAGatgactgcc                                                                                     >  4:797366/1‑149 (MQ=11)
                                                                  gggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGGTTCGGCGGGTTTGTAATTTTTGAGTGGGATAAAgtggagtgggt                                                                                    >  5:146414/1‑141 (MQ=11)
                                                                  gggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACCCGATACGGCGGGTTTATACTTTTTGAGTGCGAGaaattaaaaacac                                                                                     >  8:492552/1‑140 (MQ=11)
                                                                   ggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGCTTCGGCGGGTTTGTAATTTTTGAGTGCCAGAAAGatcaaaccctt                                                                                   >  3:846720/1‑145 (MQ=11)
                                                                         tGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTCCTTTTGGAGGGGGCGAAagaagacacccttgccccg                                                                             >  1:1514767/1‑138 (MQ=11)
                                                                         tGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTAATTTTGGAGGGGCAGATAGatcaatccctttcgtcg                                                                             >  5:1849901/1‑140 (MQ=11)
                                                                             tttGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTACGGCGGGTTTTTAATTTTGGAGTGCCATAAATATCAAGGACTGGCCACGaca                                                                          >  2:1357936/1‑150 (MQ=11)
                                                                                   ggggCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTCCTTTTGGAGGGGCAGATAGTGGATAGCCTGTCGCCGACACTCTAcc                                                                   >  3:1188518/1‑151 (MQ=11)
                                                                                   ggggCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATTCGGCGGGTTTTAAATTTTTGAGTAGCTGAAAAAAGAATCGGTGTGCAATACACtataa                                                                    >  6:674947/1‑149 (MQ=11)
                                                                                       cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAACCCGCTGCGGCGGGTTTGTACTTTTTGAGGGCCAGATAGTAGAACCGCTTTCCGCGacacatgaaccac                                                                >  1:895364/1‑141 (MQ=11)
                                                                                         gAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAGTGTCCGCGAGACtagcgccaaa                                                               >  1:1817754/1‑141 (MQ=11)
                                                                                           aaGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGAGTCGGCGGGTTTTTACTTTTTGAGTGCCAGAAAGAGGACAGACTGTCCGCGACACtagaacaaaatgc                                                            >  3:1290547/1‑139 (MQ=11)
                                                                                               tgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTACGGCGGGTTTTTACTTTTTGAGGGGCATAAATATGATAGACTTTCCACGACACTATACGCAAAAGAAAGc                                                        >  8:1554638/1‑150 (MQ=11)
                                                                                                   tgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCGATTCGGCGGGTTTTTACTTGTTGAGTGGCATAAATAGGAGTCCCTGTCCTCGGAAGTCGACCCATAAGAACTCGGCt                                                    >  1:304420/1‑150 (MQ=2)
                                                                                                    gaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCCCGACACCATACCCAAAAGCAAGCGGCtt                                                   >  7:474004/1‑150 (MQ=255)
                                                                                                        gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAATATGAGTGCCAGTCGACGACACTTTACCAAAAAGCATCCGGCTTATCgg                                              >  3:634161/1‑151 (MQ=11)
                                                                                                         tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAATGCCTTTCCGCGACACTATACTCTAAAGAAAGCGGATTATCgct                                             >  7:201227/1‑149 (MQ=11)
                                                                                                         tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGATGCGGCGGGTTTATACTTTTGGAGTGCCATAAATAAAAAAGAGATTCGACGACACTAAAGACAAATGAATGcacatgaacgg                                              >  2:228025/1‑140 (MQ=2)
                                                                                                         tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTATAATTTTGGAGGGGCAGAAATATGAAACACTGTCCACGACACTATAGACAAAAGAAAGCGGCATATCGGt                                             >  8:629558/1‑151 (MQ=11)
                                                                                                         ccccccgcccTATCCCCAACATAACGCGGCTTATCGGTCCTTTTCACCTGTTTTAGTTAAAAACCCGCTTGGGGTGGTTTTTTCTTTTGTAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCGAAAAGAAAGCGGCTTATCGGt                                             <  1:1334232/142‑1 (MQ=11)
                                                                                                              gACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTGTACTTGTGGAGGGGCATAAAGATGAGTGAGTTTCGCCGACACGATCCACAAATGAAACCGCCATATCGCTAAGtt                                        >  7:1507929/1‑151 (MQ=11)
                                                                                                               acacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGACACTAGACGCTAAAGAAAGCGTCTTATCGGTCAGttt                                       >  5:115634/1‑151 (MQ=255)
                                                                                                                cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTGTAATTTTTGAGTGCCAGAAAGATGAAACACTTTCCACGTCACTAAAGAAAAAAGAAAGCGCATTATCAGTCAGtttt                                      >  1:1791831/1‑151 (MQ=11)
                                                                                                                cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGtttt                                      >  1:1882154/1‑151 (MQ=25)
                                                                                                                 acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATTCGGCGGGTTTGTAATTTTGGAGTGGCAGATATAATTATGACTGTCGACGAGAATATACCCAAGAGACAGCGGGTTATCGATCAGtttt                                      >  8:1925536/1‑150 (MQ=11)
                                                                                                                    ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATATATTAAGAAAATCCACTATTCAAAGAGTTTTAAGTTTTGTAGAGGAATAAAGATTAATGAGTTTAATAAAAAAAATACCCAAAAGAAGGCGGGTTATCGGTCAGtttgtcgg                                  >  8:697940/1‑146 (MQ=2)
                                                                                                                     taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTTATGATTTTTGAGTGCCAGAAATAACAAAGAAAGTCAAAGACACTATAACGAAAAGAAAGATTCTTATAGGTCAGATATACCTg                                 >  4:865625/1‑151 (MQ=2)
                                                                                                                         cAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATGt                             >  1:928973/1‑151 (MQ=255)
                                                                                                                         cAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAAGAATAATGACTTTCCACGACACAATAGCAAAAAGACAGCAGCTGATCGCTCattataacattatgt                             >  4:314449/1‑137 (MQ=11)
                                                                                                                           aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTATACTTTTGGAGTGCCAGAAAGAAGAAACAGTTTCGACGACACTATAAACTAATGAAAGCGGCTTATCGGTCAGTTTTaagatatgtac                           >  8:1569464/1‑141 (MQ=11)
                                                                                                                            aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCATAAAGATGAAACACTGTCCACGACACTATACACAAAAGAAAGCGCCTTATCGGTCAGTTTTACATTTTGTACg                          >  3:430618/1‑151 (MQ=11)
                                                                                                                            aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATGTACg                          >  3:372781/1‑151 (MQ=255)
                                                                                                                              gaaagCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGGTTCGGCGGGTTTTTACTTTTTGAGGGCGAGAAATATGAATCGCTTTCGCCGACAATTGAAGATAAACTAAGCGGATAATCGGTCAGATTTACCTGatgtatgt                         >  1:104245/1‑150 (MQ=9)
                                                                                                                               aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAActgattgtcggtat                       >  5:275856/1‑138 (MQ=11)
                                                                                                                                 agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAGACAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  3:19662/1‑150 (MQ=255)
                                                                                                                                 agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  5:618582/1‑150 (MQ=255)
                                                                                                                                   cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                    >  7:613215/1‑150 (MQ=255)
                                                                                                                                    ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGCTCAGTTTTACCTGATGTACGTAATAAAc                  >  1:1106754/1‑151 (MQ=255)
                                                                                                                                    ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  7:1646709/1‑151 (MQ=255)
                                                                                                                                     cctatggggTCAGTTGCCCCTGTTTTCCGAAAAAACCCGCTTCGGGGTTTTTTTTCTTTTGGAGGGGCATAAAGATCAATCCGTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 <  7:931254/144‑1 (MQ=14)
                                                                                                                                       ttccgtgttaGTTTCACCTGTTTTACTTAAAAACCCGCTTCTGTTTTTTTTTTCTTTTTGTGGGGCATAATTATTATTCACTTTCCAGTACTCTATACCCAAAAGAAAGCGGCTTATCTGTCAGTTTTACCTGATGTACGTAATAAACCg                <  6:611925/141‑1 (MQ=9)
                                                                                                                                       ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  2:1836043/1‑151 (MQ=255)
                                                                                                                                        ttcgtttttttttAACCTTTTTTAGTTAAAAACCCTTTTCTGCGTTTTTTTTTTTTTGTTGGGTCATAATTATTAATTAATTTCCACGACTCTATACACAAAAGAAAGCGGCTTATCGTTCAGTTTTACCTGATTTACGTAATAAACCGt               <  4:1632396/140‑1 (MQ=9)
                                                                                                                                        tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  2:1195141/1‑151 (MQ=255)
                                                                                                                                          tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  2:1489985/1‑151 (MQ=255)
                                                                                                                                          tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  2:768659/1‑151 (MQ=255)
                                                                                                                                          tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  7:1046734/1‑151 (MQ=255)
                                                                                                                                           cccccAGTTCCCCCTGTTTTACGAAAAAACCCGCGTCGGGGGTTTTTTTATTTTGGCGCGCCATAACTATTATTCACTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           <  2:935901/147‑1 (MQ=9)
                                                                                                                                           cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCTGAAAGATGAATTACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  3:303679/1‑151 (MQ=255)
                                                                                                                                           cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  8:1089417/1‑151 (MQ=255)
                                                                                                                                           cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTAAgaaagaaagagcttcg           >  4:125366/1‑143 (MQ=11)
                                                                                                                                            ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  4:445991/1‑151 (MQ=255)
                                                                                                                                            ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  1:1023244/1‑151 (MQ=255)
                                                                                                                                              tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  8:1280244/1‑151 (MQ=255)
                                                                                                                                              tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  4:878126/1‑151 (MQ=255)
                                                                                                                                               cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  8:790803/1‑151 (MQ=255)
                                                                                                                                               cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  7:1800524/1‑151 (MQ=255)
                                                                                                                                               cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  6:1017680/1‑151 (MQ=255)
                                                                                                                                                  tttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  2:1671811/1‑151 (MQ=255)
                                                                                                                                                   ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGGGGGttt   >  6:544291/1‑151 (MQ=255)
                                                                                                                                                   ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGCAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGACAGTTATACCTGATGTACGTAATAAACCGTTCCGGCGGGtgt   >  2:1129193/1‑149 (MQ=255)
                                                                                                                                                     cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  1:836686/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGT‑TTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613622‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑