BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	936,169	0	T	.	29.4%	248.1 / 109.9	106	intergenic (‑13/+21)	ECDH1_RS04615/ECDH1_RS04620	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (6/66); new base . (5/25); total (11/95)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 130 total aligned reads displayed.

AATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGG  >  DH1/936020‑936310

aatgcggcTTTTTTTTGGGAGACCCGAAACAAAAACAGCCGCGTAAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                               <  3:1575640/145‑1 (MQ=17)

aaaggCGCGTGTTTAGGCGAGCCCCGAACCAAAAAAACACCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                               <  1:1435037/148‑1 (MQ=17)

aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                               <  1:1105163/151‑1 (MQ=255)

aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                               <  8:167019/151‑1 (MQ=255)

aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                               <  6:869242/151‑1 (MQ=255)

 aTGGCGTTTTTTTATGGGAGACGAGAATCTATATATCTCCCGTTAGGGTGTTGATTATTTGGTTGCGGGGTGGGGATTTGAACGGAGGAGCTTCGGGTTATGAGCCCGACGAGCTACGAGGCTGCTCCACCCCGCGTCTGCGTAGTGCtt                                                                                                                                               <  8:353857/150‑1 (MQ=12)

 aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                              <  3:250308/151‑1 (MQ=255)

 aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                              <  6:811030/151‑1 (MQ=255)

 aTGGCGCTTTTTTATGGGAGACCACAACCAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                              <  7:290760/151‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCATAAAAAAAAAAACACCCGTTAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                             <  6:1331920/150‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                             <  7:364108/150‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                             <  5:1000531/150‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                            <  1:26215/151‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                            <  2:1064047/151‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                            <  5:21700/151‑1 (MQ=255)

   ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                            <  6:638289/151‑1 (MQ=255)

    gctcTTTTTTTTTGGAGACCAGATACAATTAATGATTCTTTAGGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttact                                                                                                                                           <  2:1701503/148‑4 (MQ=14)

    gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                           <  1:756406/151‑1 (MQ=255)

     gtcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                          <  8:4546/149‑5 (MQ=255)

     ggcTTTTGTATGGGAGCCCCGTACCCACAACAGACCGGAGAGGCTGTTAAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAGCCCGCGTCACCGTACTGCtttactt                                                                                                                                          <  5:1289133/150‑5 (MQ=11)

     cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                          <  3:664492/151‑1 (MQ=255)

     cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                          <  1:1582237/151‑1 (MQ=255)

     cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGCTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                          <  3:1682540/151‑5 (MQ=255)

      gcgtttttATGGGAGACCAGAACAAAAAAAACACCCTATAGGTTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                        <  4:368682/148‑1 (MQ=17)

      gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGTGTGTTCAATAATTGTTTGCGGGGGCCGGAATTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTACTGCTTTTACtt                                                                                                                                         >  7:1902992/1‑151 (MQ=255)

      gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                         <  5:1534451/151‑1 (MQ=255)

       gttttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                        <  1:1793088/150‑1 (MQ=255)

         ttttttttGGAGACCTGTAACAATAAAAGCCGTTATAGGGTGTTCATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                       <  8:665350/149‑1 (MQ=11)

         tttttATGTGAGACCAGAAAGAAAAAATCTCCCGTTAGTGTGTTCAATAATTGGTTGCGGGGGCGGGATTTGAAGCGACGACCTTCGGGTTATGAGGCCGAAGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                         <  2:934272/148‑1 (MQ=18)

         tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                        <  5:889249/149‑1 (MQ=255)

         tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcat                                                                                                                                      <  5:23745/150‑1 (MQ=255)

          ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                      <  5:685058/150‑1 (MQ=255)

          ttttATGAGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                      <  6:1730185/150‑1 (MQ=255)

           ttttatggaccccAGAAACAAAAAAACACCCTTCAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                    <  7:797818/139‑1 (MQ=17)

           tttAAGGGAGACCACAAACAAAAAAACACCCGATAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                    <  7:1787388/150‑1 (MQ=17)

            ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                    <  5:1082946/150‑1 (MQ=255)

            ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGCTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                    <  3:1247038/150‑1 (MQ=255)

             tATGGGAGACCAGAACCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                   <  2:423816/150‑1 (MQ=255)

             tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                   <  1:370868/150‑1 (MQ=255)

             tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                   <  8:1706545/150‑1 (MQ=255)

             tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                   <  4:1882961/150‑1 (MQ=255)

              aTGGTAGACCAGTAAAAAAAAAACAGCCTTTTTGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑t                                                                                                                                <  8:649032/150‑1 (MQ=14)

              aTGGGAGCCCAGAACCAAAAAAAGACCGGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                               <  5:683476/151‑1 (MQ=17)

              aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                  <  7:1382402/150‑1 (MQ=255)

              aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                <  5:123689/151‑1 (MQ=255)

                gggAGACCCCAAACAATAAAACAGCCGTTAGGGTGTAAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                              <  1:1090841/150‑1 (MQ=14)

                gggAGACCAGAAACAAAAAACCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                              <  3:239263/151‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCTGTTAGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                              <  2:745186/150‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                              <  3:1384167/151‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                              <  2:1230254/151‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                              <  8:387472/151‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                              <  1:1611724/151‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                               <  2:662621/150‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                               <  1:1746294/150‑1 (MQ=255)

                gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                               <  1:831018/150‑1 (MQ=255)

                gcgagaCCAGAACAAAAAAAACCCCGTTTAGGTTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                              <  5:15876/148‑1 (MQ=14)

                 ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                             <  7:1030738/151‑1 (MQ=255)

                  gagaCCAGAAACAAAAAACCACCCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGCTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGa                                                                                                                            <  7:1738097/151‑1 (MQ=255)

                  gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGa                                                                                                                            <  7:96173/151‑1 (MQ=255)

                   agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAt                                                                                                                           <  1:1315826/151‑1 (MQ=255)

                    gaCCAGAAACAATAAAACACCCGTTTGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGGCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAt                                                                                                                           <  1:1013747/150‑1 (MQ=255)

                    gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                          <  1:1866680/151‑1 (MQ=255)

                    caCCAGAAAACAAAACACCGCGGTTAGCGTGTACAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                         <  3:1368838/150‑6 (MQ=1)

                     aCCAGTAAAAAAAAAAGACTCTTTTTGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                         <  4:1200828/150‑6 (MQ=1)

                     aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACGTCATTCAA‑ttttaaatc                                                                                                                        >  1:318761/1‑145 (MQ=17)

                      ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                        <  1:1680393/151‑1 (MQ=255)

                       aaGAAACAAAAAAACACCCGTTAGGGTGTTCAATATTTGGTTGCGGGGTCCGGATTTGAACCGACGACCTTCGGTTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAACGTACTGCTTTTACTTCATCAAA‑TTTGATTGGt                                                                                                                       <  4:1528128/150‑1 (MQ=255)

                        cgcaacaacaacACCCCCGAGAGGGTGTTCAATCATTGGTGGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                      <  7:480253/148‑1 (MQ=11)

                         gattaaaaaaaaCACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                               <  4:1048109/135‑1 (MQ=255)

                         gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                     <  7:1411764/151‑1 (MQ=255)

                         caaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                     <  2:379484/150‑1 (MQ=255)

                         atcgcAAAAACACCCCGGTCAGGGTGTTCAATCATTGGTTGCGGGGTCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTg                                                                                                                     <  1:1796672/147‑1 (MQ=1)

                          aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                    <  4:1749342/151‑1 (MQ=255)

                          aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                    <  8:28509/151‑1 (MQ=255)

                           aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTTCTTCATCAAA‑TTTGATTGGTTGCg                                                                                                                   <  7:1807160/151‑1 (MQ=255)

                           aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCg                                                                                                                   <  5:446521/151‑1 (MQ=255)

                            aaaataaaaCACCCTTTAGGTTGTTCAATAATTCGTTCCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCgg                                                                                                                  <  4:1554761/146‑1 (MQ=11)

                             cAAAAAAACACCCGTTAGTGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACGAGTCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggg                                                                                                                 <  8:1900851/151‑1 (MQ=255)

                             cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggg                                                                                                                 <  2:940841/151‑1 (MQ=255)

                             cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAAACGACGACCTTCGGGTTATGAGAACGACGAGCTACCAGGCTGCTCCACACCGCGTAACCGAACTGC‑TTTACGTAATCAAATTTTAATTGGTTg                                                                                                                     >  2:1799314/1‑147 (MQ=14)

                             cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAAACGACGACCTTCGGGTTATGAGAACGACGAGCTACCAGGCTGCTCCAAACCGCGTCACAGTACTGC‑TTTACGTCATCAAATTTTAATTGGTTgagct                                                                                                                 >  6:978559/1‑147 (MQ=14)

                              aataaatcatgttttAGGGTGTTCAATAATTGGTTGTTGGTGCTGTATTTTAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggg                                                                                                                 <  2:1756778/137‑1 (MQ=17)

                              aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                                <  8:15352/151‑1 (MQ=255)

                               aaaaaaCACCCGTTAGGGTGTTCAATAATTGGCTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                               <  8:253839/151‑1 (MQ=255)

                                aaaaaCACCCGTTAGGGTGTTCAATAATTGGTGGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCGCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                              <  2:547180/151‑1 (MQ=255)

                                aaaaaCACCCGTTAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCGTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                              <  7:589256/151‑1 (MQ=255)

                                 aaaaCACCCGTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGAGTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCGTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGcc                                                                                                             <  3:379205/151‑1 (MQ=255)

                                   aaCACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                        <  5:1611866/137‑7 (MQ=255)

                                   aaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTAATTAATAAAA‑ttttaattg                                                                                                                        >  6:1611420/1‑131 (MQ=14)

                                     cacCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                         <  4:1776604/134‑6 (MQ=11)

                                     cacCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                         >  3:1773274/1‑129 (MQ=255)

                                                             ggTTGCGGGGGGCGGATTTGACCCGACCAGCTTGGGGATATCCGCGCGACGAGCATCGTGGCTGGTCCACCCCTCGTCACCGAACTGCTGTTTCTTCATCATA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                  <  7:84283/150‑1 (MQ=11)

                                                                                   cgttcgtggttcgttttATGAGCCCGACGAGGTTCGATTTTTCTCGACCCCGGGTCAGCGTACTGCTTTTTGTTGATGATT‑TTTTATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgct                                                           <  8:1089381/136‑1 (MQ=2)

                                                                                                   tATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGGTATTAAACAAGGAAA‑TTTTATTGGTTGAGGGGTCATTATTTGACCCGCCCACCTTAGGGTTATGAGACAGCCGAGCTACCAGGCTGCTCCACACCGCGTcc                                           >  7:96042/1‑150 (MQ=2)

                                                                                                                          ctgctCCACCCCGCGTCACCGTACTGCTTTAATTTCATAAAT‑TTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgct                                                        <  1:172876/115‑1 (MQ=11)

                                                                                                                           tgctCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGcc                                                                            >  1:113421/1‑94 (MQ=255)

                                                                                                                             ggccACCCCTCGTCCCCGTAATGCCGATACTTCCTCAAA‑ATTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                 <  5:534724/149‑1 (MQ=14)

                                                                                                                                cACCCCGCGTCACCGTACTGCTTTTAGTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACGGTACTCCGTGACTACATCaatgggttt              >  8:1084776/1‑144 (MQ=14)

                                                                                                                                          ccccagacagCTCTTTCTTCGTCTAA‑ATTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCgg    <  3:800833/142‑1 (MQ=14)

                                                                                                                                            ccGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGCGTACTGCGTTACTTCATCAAATTTTATTTGGTTGCgggg  >  7:426453/1‑151 (MQ=255)

AATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGG  >  DH1/936020‑936310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`