Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,326,2870GT25.9% 1001.1 / 97.7 490intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand):  ref base G (221/129);  new base T (32/90);  total (257/233)
Fisher's exact test for biased strand distribution p-value = 1.48e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 496 total aligned reads displayed.

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437
                                                                                                                                                      |                                                                                                                                                      
 aTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGa                                                                                                                                                       <  8:778728/151‑1 (MQ=33)
          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACagatg                                                                                                                                               >  4:503125/1‑150 (MQ=18)
              aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaa                                                                                                                                           <  2:500785/150‑1 (MQ=255)
               tCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTTAT‑ACaaaa                                                                                                                                         >  6:518433/1‑149 (MQ=255)
               tCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACaaa                                                                                                                                          >  6:979989/1‑150 (MQ=255)
                cAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGCAAAAcc                                                                                                                                        >  7:1530668/1‑150 (MQ=255)
                cAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAAc                                                                                                                                         <  3:1491583/150‑1 (MQ=255)
                cAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACg                                                                                                                                        <  3:1097573/151‑1 (MQ=255)
                  ggCCTACACGTTAACTGCTATCTATTGTATTTGAAGGGTTTTCATTCCGTATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGc                                                                                                                                       <  2:1269676/150‑1 (MQ=255)
                     cTACACGTTCACTGCAATCTAGTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCGTTTACAGCGCCATTTGAAACTGATGACAAACGCAAc                                                                                                                                    >  5:168811/1‑150 (MQ=255)
                       acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACa                                                                                                                                   <  3:1198980/149‑1 (MQ=255)
                       acacGCTCACTGCAATCTATTGGATTTGAAGGGTTTTCATTCCTTATAAGGCGTTCACGCCGCATCGGGCATGAACAAAGCGTACGTTGTCTACAATATGAAAATTACGACCTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAtt                                                                                                                                 <  5:1115027/151‑1 (MQ=17)
                           gTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATAAAAAAAAAAACAtttaat                                                                                                                             >  6:28544/1‑147 (MQ=11)
                           gTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCCGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATCACGCCTATTACAGCGCCATTTAAAAATCATAACAAACTCAACAATGca                                                                                                                              >  4:1243765/1‑149 (MQ=11)
                             tCACTGCAATGTATTGTATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTg                                                                                                                            <  3:483507/150‑1 (MQ=255)
                             tCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTg                                                                                                                            <  4:917125/150‑1 (MQ=255)
                                cTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgg                                                                                                                        >  3:1437687/1‑150 (MQ=255)
                                 tGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGCCGTTCGAGCAGCATCCGGCAGGAAAAAAGCCAACTTAGTCTACAATATGAAAATGCCGCCGGTTACAGATCCATTTGAAACGGCTGGCAACCGCACCATTGCCTGATgcg                                                                                                                       >  1:715515/1‑151 (MQ=14)
                                   aaaTCTATTGGATTTGAATGTTTTTGATGCCGGTTAAGTCTTTAAAGACGGATCCGGCATGAACAAAGCCTACTTTGTCTACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                     <  6:1122999/150‑1 (MQ=18)
                                      tCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACg                                                                                                                  >  3:134756/1‑151 (MQ=255)
                                      tCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAAAGCAACATTTACTGATGCGCTACg                                                                                                                  >  5:563690/1‑151 (MQ=255)
                                        tattgtcgttGACGGGTGTTGATGCCGGTTAAGGCTTTCACGCCGCATCGGGCATGAAAAAAGCCTTCGTTTTCTACAATATGAAAATGACTCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGc                                                                                                                 <  6:159579/142‑1 (MQ=25)
                                           tGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAACCTGATGACAAACGCAACATTGCATGATGCGCTACGCTTAt                                                                                                             >  5:1407374/1‑151 (MQ=255)
                                           tGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTTCCTGATGCGCTACGCTTAt                                                                                                             >  3:248329/1‑151 (MQ=255)
                                            tgATTTGTAGGGTTTTGATTGCCTTTAAGTCTATAACGCCGCATCCTGCATGAACAAAGCCTACGTTGTATACAATATGAAAATGACTCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTAt                                                                                                             <  6:1838353/149‑1 (MQ=21)
                                             gATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATcc                                                                                                           >  3:816592/1‑150 (MQ=255)
                                             gATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCa                                                                                                           <  8:323186/151‑1 (MQ=255)
                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAg                                                                                                          <  8:13033/151‑1 (MQ=255)
                                                 tGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                       >  4:1691347/1‑151 (MQ=255)
                                                   acgggTTTTCAAGCCGCATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACCCCTTATACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                                     <  3:1052117/149‑1 (MQ=255)
                                                   aaGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAAAAACGCAACATTGCCTGATGCGCTACGCATATCAGGACt                                                                                                      >  8:547095/1‑150 (MQ=255)
                                                       gTTTTCATGCCCGATAAGGCGTTCACCCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATAACAAAATGACGCCTTTTACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                 <  7:1282997/151‑1 (MQ=255)
                                                           tCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                              <  8:925907/150‑1 (MQ=255)
                                                           tCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                              >  7:1797814/1‑150 (MQ=255)
                                                            cATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAc                                                                                            >  1:1370794/1‑151 (MQ=255)
                                                            cATGCCGGATAACGCTTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATTAAAATGCCGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAc                                                                                            <  5:1584766/151‑1 (MQ=255)
                                                              tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAGGCCTCCGTTGGGTCCATTATGAAATTGCGGCCTTTTCCAGCGCAATTTGAACGTGAGGCCAAAGGAACCATTGCGTCAGGCGGTCCGGTTAGCGGGCGTCCAGGTGCACTg                                                                                          >  3:1799238/1‑151 (MQ=2)
                                                              ggCCGGATAAGGCGGTCACGCCGCATCCGGCATTAACAAAGCCTACGTTGTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTg                                                                                          <  1:100775/150‑1 (MQ=255)
                                                               gCCGGATCAGGCTTTCACGCCGCAACCGGCATGAACAAAGCCTTCGTTGTCTACAATATGAAAATGCCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         <  5:209453/151‑1 (MQ=255)
                                                               gCCGGATAAGGCGTTCACGACGCATCCGGGATGAAGAAAGCCTACGTTGTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTg                                                                                          <  8:1598986/150‑1 (MQ=255)
                                                                acGGATCAGGCGTTCACGCCGCATCCGTCATGAACAAAGCCTACGTTGTATACAATATCAAAATGCCGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                        <  5:1799693/150‑1 (MQ=25)
                                                                 cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                        >  5:846444/1‑150 (MQ=255)
                                                                  ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGAAACATTGACTCATGAGCTACGCGTATCAGGCCTACACGTTCACTGCAAt                                                                                      >  6:1081527/1‑151 (MQ=21)
                                                                  ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                      >  6:202822/1‑151 (MQ=255)
                                                                   gATAAGGGGATCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATc                                                                                     <  1:1581157/151‑1 (MQ=255)
                                                                    aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTAGAAACTGATGACAAACGCAACATTCCCTGATGCGCTACGCTTATCAGCCCAACACGTTCACTGCAATCt                                                                                    >  8:177169/1‑151 (MQ=255)
                                                                     acagtctttCACGCCGCTTCCGGCATGAACAAAGCCTTCGTTGTATACAATATCAAAATGACTCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTa                                                                                   <  2:144130/144‑1 (MQ=18)
                                                                     aacggCTTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACCCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTa                                                                                   <  3:658681/148‑1 (MQ=255)
                                                                      aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAt                                                                                  >  7:1181766/1‑151 (MQ=255)
                                                                         gCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                               >  7:985187/1‑151 (MQ=255)
                                                                           gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                             >  2:1207705/1‑151 (MQ=255)
                                                                           gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                             >  7:1054982/1‑151 (MQ=255)
                                                                            ttCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCGACGCTTATCAGGCCTACACGTTCCCTGCAATCTATTGGAtt                                                                            >  7:887557/1‑151 (MQ=255)
                                                                             tCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAttt                                                                           >  7:1613787/1‑151 (MQ=255)
                                                                              aactcctcatacttcATTAATAAAGCCTTCGTTTTATTCAATATTAAAATGTCGCTTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAttt                                                                           <  6:810268/136‑1 (MQ=14)
                                                                                cgccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATTCGATACGCTTATCAGGACTACACGTTCACTGCAATCTATTGGATTTGa                                                                         >  2:8066/1‑150 (MQ=255)
                                                                                 gccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGa                                                                         >  5:1612062/1‑149 (MQ=255)
                                                                                 cccgcATCCGGCATGAACAAAGCCTTCTTTGTCTACAATATCAAAATGACCCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAg                                                                       <  5:460953/150‑1 (MQ=255)
                                                                                   cgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTTATGAAAAAAACAACATACCCTCATCCTCTACGCAAATCATGCGTACAAGTTCAGTGAAATCTATTGGAGTTGAAgg                                                                      >  4:356272/1‑150 (MQ=18)
                                                                                   cgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAggg                                                                     >  1:607539/1‑151 (MQ=255)
                                                                                    ggctCCGGCATGAACAAAGCCGACGTGTTCTACAATATGAAAATGCCGCCTTTTACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGt                                                                    <  5:128083/148‑1 (MQ=18)
                                                                                        ccGGCATGAACAAAGCCTACGTTGTATACAATATTAAAATGTCGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTc                                                                <  6:1750493/151‑1 (MQ=255)
                                                                                             aTGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTCATGCCCTACGATTATCAGACCTACACGTACACTGCAATCTATTGGATTTGAAGGGTTTTCATgac                                                           >  8:711504/1‑149 (MQ=21)
                                                                                                  cAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCg                                                          <  7:501777/147‑1 (MQ=255)
                                                                                                   aaaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGACTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                      >  4:1741405/1‑150 (MQ=255)
                                                                                                    aaGCCTACGTTGTCTACAATATGAAAATGCCTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAg                                                    <  1:1859137/151‑1 (MQ=255)
                                                                                                    aaGCCTACGTTGTCTACAATATGAAAATGACGCTTTTTATATATCAATTTGAAACTTATGACAAAATCAATATTGTTTTATGAGTTACGCTTATCTTGCCTATTCGTTTATTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAg                                                    <  2:715797/151‑1 (MQ=12)
                                                                                                       ccTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCg                                                 >  4:1435461/1‑151 (MQ=255)
                                                                                                         tACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGtt                                               >  8:111171/1‑151 (MQ=255)
                                                                                                            gTTGTCTACAATATGAAAATGACGCCATATACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAc                                            <  3:940677/151‑1 (MQ=255)
                                                                                                             ttttatACAATATTAAAATGACGCATTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAc                                            <  3:1622813/145‑1 (MQ=14)
                                                                                                             ttGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcg                                           >  6:74031/1‑151 (MQ=255)
                                                                                                             ttGTATTCAATATTAAAATGTCTCTTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCa                                             <  6:1644951/149‑1 (MQ=11)
                                                                                                               gTCTACAATAAGAAAATGGCCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                          <  1:837051/150‑1 (MQ=255)
                                                                                                                   aCAATATCAAAATGACCCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                     <  5:306889/151‑1 (MQ=255)
                                                                                                                    cAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATc                                    >  4:824145/1‑151 (MQ=255)
                                                                                                                    cAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATc                                    >  1:388598/1‑151 (MQ=255)
                                                                                                                     aaTATTAAAATGTCGCGTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                   <  8:233245/151‑1 (MQ=255)
                                                                                                                     aaTATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgcggctacc                                   >  8:1396852/1‑147 (MQ=255)
                                                                                                                      atatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCg                                  >  2:1462319/1‑151 (MQ=255)
                                                                                                                       tatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                 >  4:600443/1‑151 (MQ=255)
                                                                                                                        atGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGACGGATAAGGCGTTCACGCCGCATCCGGc                                >  6:1356265/1‑151 (MQ=255)
                                                                                                                          gAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                              >  2:236681/1‑151 (MQ=255)
                                                                                                                          caaaaTGCCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                              <  5:1881160/150‑1 (MQ=255)
                                                                                                                           aaaaTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                             >  1:1257624/1‑151 (MQ=255)
                                                                                                                                gACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa                        <  7:1361443/151‑1 (MQ=255)
                                                                                                                                   cccTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGc                     <  1:1091117/150‑1 (MQ=39)
                                                                                                                                     cTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   >  2:1598922/1‑151 (MQ=39)
                                                                                                                                      ttttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   >  7:542632/1‑150 (MQ=38)
                                                                                                                                      ttttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   >  8:1852612/1‑150 (MQ=38)
                                                                                                                                         tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt               >  4:966434/1‑151 (MQ=35)
                                                                                                                                          aCATCGCCATTTTAAACTTATTAGAAAAGCAATATTTCCTTATTCGCTACTCTTATGATGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt               <  6:168473/150‑1 (MQ=11)
                                                                                                                                          aCAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGtt              >  3:324600/1‑151 (MQ=35)
                                                                                                                                           cAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCGGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTg             >  2:1821640/1‑151 (MQ=21)
                                                                                                                                            aGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGt            >  7:655574/1‑151 (MQ=33)
                                                                                                                                               gcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCt          >  5:676672/1‑150 (MQ=33)
                                                                                                                                                ccATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc        <  7:452711/151‑1 (MQ=33)
                                                                                                                                                 cATTTGAAACTGATGACAAACGCAACATTGCCTTATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTTGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACa       <  8:1180730/151‑1 (MQ=18)
                                                                                                                                                  aTTTGAAACTGATGACAAATGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACa       >  7:256580/1‑150 (MQ=18)
                                                                                                                                                      gAAACTGATGCCATAGGTAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAatat   <  4:1565555/150‑1 (MQ=9)
                                                                                                                                                      |                                                                                                                                                      
GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑