Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA26.2% 308.2 / 44.7 149intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (73/37);  new base A (35/4);  total (108/41)
Fisher's exact test for biased strand distribution p-value = 6.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

CGAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184435‑3184734
                                                                                                                                                      |                                                                                                                                                      
 gAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTc                                                                                                                                                        >  8:757880/1‑150 (MQ=255)
       cgtcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAgcagc                                                                                                                                                 >  7:1636586/1‑147 (MQ=255)
         tcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGaacctt                                                                                                                                               >  7:1472111/1‑146 (MQ=255)
            tAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGCTTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCGACTTTATTAAAGCGGC‑TTAcc                                                                                                                                           >  8:890869/1‑151 (MQ=25)
            tAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTAATAAAgcaccctacc                                                                                                                                            >  7:701581/1‑142 (MQ=21)
               ccaccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTg                                                                                                                                        >  3:30154/1‑151 (MQ=255)
                 accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGATCG‑TTACCTAGt                                                                                                                                       >  1:239687/1‑150 (MQ=21)
                    aaaTTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGt                                                                                                                                   <  1:1207593/151‑1 (MQ=255)
                       acgttactttatcctatatGTTCATGAATTTCACATCCATTTTTTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCa                                                                                                                                <  7:170311/136‑1 (MQ=18)
                          tGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTCCCGTGTAAGGGCAccc                                                                                                                             >  7:79202/1‑151 (MQ=255)
                            aaTGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCAGGCATTCCGAGGTTCGAATCAACGTACCCCAGCCAATTTATAAAAGATGC‑ATACCtagatatttatccaat                                                                                                                           >  2:1345890/1‑136 (MQ=21)
                                tATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGCACC‑TTTCGTTGTAAGTgatccgattttg                                                                                                                       >  5:547542/1‑140 (MQ=18)
                                        atGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCg                                                                                                                <  4:1089914/150‑1 (MQ=255)
                                              ggCTAATTCAAAACCTATTTGTTGGTGTATCGCCAAGCTTTAAGGAACATGATTCTGATTCCTGCATTAAGAGGTTCGAATCCTCGTACACCAGCCAATTTATTCAAGAAGC‑TTACCTTGTAAGTGCACCAAGTTGGGGTATCGCCAAGCg                                                                                                         <  1:419730/151‑1 (MQ=255)
                                                aaaaaTCAAAACCAATTTGTTTGGGTATCGCCAAGCTGTAAGGCAACTGATTCTGATTCCGGCATTCAGAGGTTCGAATCCTCGTACCCCAGCAAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGt                                                                                                       <  8:1235013/150‑1 (MQ=37)
                                                 aaaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTAATAAAGCCCC‑TTTCGTGGTGAGTGTAGCGAGTGTGGGTAGCGGCaatctgt                                                                                                       >  1:518871/1‑145 (MQ=14)
                                                 aaaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTTCGTTGTAAGTGCACCCTGTTGGGGTAGCGCCAAGCGGt                                                                                                       >  7:394806/1‑150 (MQ=255)
                                                 aaaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTCAAGCCGC‑TTTCGGAGTAAGTGGTCCGAGTTGGGGTAGCGCCAAGCggg                                                                                                       >  5:832005/1‑149 (MQ=18)
                                                  aaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAAAAAAGCGGG‑TTACCTTGACATTTCTCCCAGTAGGGGTACAGGCAAGCGCTa                                                                                                      >  5:1235795/1‑150 (MQ=14)
                                                  aaTTCAAAACCAATTTCTTGGGGTATCGCCAACCGGTAAGGCACCGGATTCTGAGTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTaa                                                                                                     <  7:1812929/151‑1 (MQ=255)
                                                    ttCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTTCACCGAGTTGGGGTTTCTCCAAGCGGTAAgg                                                                                                   >  7:1041217/1‑151 (MQ=255)
                                                          gttgtacggatgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCATTTTATTCAAGACGC‑t                                                                                                                                               >  1:974805/11‑101 (MQ=11)
                                                              ggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGc                                                                                                                                 <  5:896730/106‑1 (MQ=255)
                                                               cacgcccctccctccccAACCCGTAAGGCACCGGATTCTGATACCGGCATTAATATTTTCGAATCCTCGTACCACAGACAATTTATTAAATAATC‑TTACATTTTAACTCCACCAAGTTGTTGTATCGCCAAGCGGTAAGGCACCGGATTCt                                                                                        <  8:340346/136‑1 (MQ=255)
                                                                ttgttgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGACCC‑GTACCGGGTTAGTGCACCCAGTTGGGGTATCGCCAAGCTGTAAGGCCCAGGAGTCt                                                                                        >  7:59226/1‑150 (MQ=18)
                                                                ttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTATGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAg                                                                                                                                    >  4:998467/4‑106 (MQ=255)
                                                                   ttgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGC‑TTACCTTga                                                                                                                                       >  1:1522610/1‑99 (MQ=17)
                                                                   ttgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAG‑CGCGGTACCTGGTGCTTTATCCGAGTTGGGGTAGCGGCAAGCGCGAAGGCCCGGGAGTCtcctg                                                                                    >  1:826075/1‑147 (MQ=0)
                                                                    tgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAAATTATTCAAGAAGc                                                                                                                                                 >  8:1146038/1‑90 (MQ=11)
                                                                    tgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGc                                                                                                                                                 <  7:1147107/90‑1 (MQ=255)
                                                                      tcttttttcctatctgTAATGCACCGGATTTTGATTCAGGCATTCCTTGGTTCGAATACTCGTACTGCAGCCAATTTATTCAAGACTC‑TTAACTTGTAATTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTcc                                                                                  <  4:599746/135‑1 (MQ=25)
                                                                      gggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGCCGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGGCAAGCGGTAAGGCACCGGATTCTACTTCCg                                                                                 >  5:1490979/1‑151 (MQ=255)
                                                                        gTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAAGA‑TTACAGTGTAAGTGCACCCAGTTGGGGTATCGCCa                                                                                                             >  2:835859/1‑121 (MQ=18)
                                                                        gTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCa                                                                                                             <  1:835211/121‑1 (MQ=255)
                                                                         tATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAAATAATTCAAGACGG‑TTACCTTGTAAGTGCACGCAGTTGGGGTATCGCCAAg                                                                                                           >  3:1306700/1‑122 (MQ=21)
                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTg                                                                                                                                  <  7:1285179/98‑1 (MQ=255)
                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGt                                                                                                                          <  4:145335/106‑1 (MQ=255)
                                                                           tgacaaatcgtcaaGGCAACGGATGCTGTGTCCGGCATTCCGAGGTTCGAAGCCACGTACCCCAGCCAATTTATTCAAGACGC‑GTACCTTGTAACTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCAt                                                                             <  7:929616/138‑1 (MQ=37)
                                                                            cGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCtt                                                                                                                                         <  6:1278711/89‑1 (MQ=255)
                                                                            cGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCACGTACCCCAGCCAATTTATTCAAGAAGC‑TTACCtt                                                                                                                                         >  5:1279623/1‑89 (MQ=11)
                                                                             ccccaccggcacgccACCGGATGCAGACACCGGCACGCCGAGGTTCGAATCCACGTAACCCAGCCAATATATTCAATTCTC‑TTACCTTGTACGTGAAACCTGTTGGGGTATCCCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTcc                                                                          <  5:1145887/137‑1 (MQ=16)
                                                                             ccacatcgttAAGGAACATGATTCTGTCACAGGCATTCCTAGGTTCGAATCCTCGTACCTCAGAAACTTTATAAAAGACGC‑TTAACTTTTAATAGCACCTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTcc                                                                          <  8:252494/142‑1 (MQ=16)
                                                                              ccAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGACCC‑TTACCTTGTAAGTGCACCCAGttcgccg                                                                                                                    >  3:1518747/1‑103 (MQ=17)
                                                                              ccAAGCGGTAAGGCACCGGTTTCTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 <  1:1694217/111‑1 (MQ=255)
                                                                                 aGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCa                                                                                                                                                       >  6:756874/1‑71 (MQ=14)
                                                                                       aaGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCACGTACCCCAGCCAATTTATTCAAGACGA‑TTACATTGTAAGTGCACCCAGTTGGGGTATCGCCAAGAGGTAAGGc                                                                                                  >  2:1662201/1‑117 (MQ=21)
                                                                                           cACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGAGCC‑TTACCTAGTAAGTGCACCGAGTGTGGGTATCGGCAAGCTCTAAGGCGCCGGAGACTACTTGCGGCATTcacagtagcgacgcct                                                            >  3:1115451/1‑136 (MQ=16)
                                                                                            aCCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGAACC‑TTACCTAGTAAGTGAACCAAGTAGGGGTATCTACAAGCTCTAAGGCAAAGTATTCAAATTACGGCATTCCGAGTTTCTAATCCTc                                                           >  4:1252296/1‑151 (MQ=16)
                                                                                                 aTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTAGGGGTAGCGCCAAGCGCTAAGGCCCCGGAGTCTAATGCCGGCATTCCGAGGGTCGAGTCCGCGTAcc                                                      >  1:1865708/1‑151 (MQ=38)
                                                                                                   tCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGG‑TTTCCTGGTGATTGCAGCGAGTTGGGGTATAGGCAAGCGGTAAGGCCAGGGAGTCTAGTTCGGGCATTCAGAGTCTGGAGTCCTCGTcccgc                                                    >  1:520441/1‑149 (MQ=16)
                                                                                                      gATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTGAGTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCCCCGGAGTCTCCTTCCGGCATTCCGAGGGTCGAGTCCTCGTCCCCCCGc                                                 >  3:503747/1‑151 (MQ=37)
                                                                                                       aTTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCCGC‑TTACGTTGAAAGTTGACCAAGTTGGCGTATAGGCAATCGCTAATGCGCAGGAGTCTAGTTGCGGCCTTCCGAGTAGCGAATCCGCCTCccccctcc                                                >  7:1882383/1‑147 (MQ=12)
                                                                                                         tctggtacgactcggTTCGTAGCCTCGTACTTCTGACTTTTTATTATAGTCTC‑TTAAATTGTAACTGCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCca                                               <  6:1880138/137‑1 (MQ=0)
                                                                                                             gCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGC‑TTACCTTGTAAGTTCACCCAGTTGGGGTATCTCCAATCGCTAAGGCACAGTATTCTATTTCCGGCATTCCTAGGTTCGAATCCTCGTACCCCAGCAACAtt                                           >  4:607201/1‑150 (MQ=37)
                                                                                                               aatacGAGCTTCGAATCCACCTACCGCAGCCTATATATTCTAGCCGC‑GTACCTTGTAATAGCAACGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaaa                                        <  7:231175/147‑1 (MQ=17)
                                                                                                               aTTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGCCGC‑GTACCGTGTAAGTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCCTTCCGAGGTTCGACTCCTCGTACCCCAGCGACATTaa                                         >  1:1887902/1‑150 (MQ=25)
                                                                                                               aTTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGCCGC‑TTACCTTGTCAGTGCACCCAGTAGGGGTATCGCCAAGCGGTAAGGCCCGGGAGTCTAATGCCTGCATTCAGAGGTTCGACTCAACGTACCCCAGCCACATTaa                                         >  7:612831/1‑150 (MQ=25)
                                                                                                                ttCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAGGC‑GTACCTGGTAAGTGCACCCAGTAGGCGTATCGCCAAGCGGTAAGGCCCCGGATACAAATGCCCGCATTCAGAGTGTCCTCCCCTCGTACCCCAGCCACATTaaa                                        >  3:1654464/1‑150 (MQ=255)
                                                                                                                 tCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGAACC‑TTACGTTGTAAGTTAACCGAGTATGCGTAACGACAAGCGCTAATGCCCCGTAGACAAATGAACGCATTCACAGTATGGAAACCGCCTACCCCAGCAACATAaaaa                                       >  8:214751/1‑150 (MQ=2)
                                                                                                                   cGAGGTTCGAATCCTCGTACGCCAGCCTCTTTATTCACGCCGC‑TTACCTTGTAACTGCACGGTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAg                                    <  7:432595/151‑1 (MQ=21)
                                                                                                                     aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGTCATTCAGAGTTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCt                                  >  1:668941/1‑151 (MQ=255)
                                                                                                                     aGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAACC‑TTACCTTGAAAGTGAACCAATTTGGGGTATCGACAAGCTCTAAAGCAAAGTATTCTAATTAAGGCATTCCTAGTATCGAATCATCCTACCCCATCAACATTAAAAAAGCt                                  >  8:525154/1‑151 (MQ=255)
                                                                                                                         tCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGC‑TTACCTTGTAAGTGAACCCAGTTGGCGTATCGCCAAGCGGTAAGGCCCCGGATACTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCt                              >  8:539955/1‑151 (MQ=255)
                                                                                                                          cGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCtt                             >  5:482560/1‑151 (MQ=255)
                                                                                                                           gAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTAGCTTc                            >  2:402596/1‑151 (MQ=255)
                                                                                                                              tCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         >  1:1492855/1‑151 (MQ=255)
                                                                                                                               ccTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  3:691066/1‑151 (MQ=255)
                                                                                                                               ccTCGTACCCCAGCCAATATATACTAGTCGC‑TTACCTTGTAAGTGCACCCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         <  8:1698248/150‑1 (MQ=255)
                                                                                                                                cacGTACCTCAGACTATATATTAAAGGCGC‑TTAACATGGAAGACCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGa                       <  7:725216/149‑1 (MQ=18)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCTCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCAGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgc                          >  3:971988/1‑147 (MQ=255)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  2:1857743/1‑150 (MQ=255)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  8:277628/1‑150 (MQ=255)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  4:1574870/1‑150 (MQ=255)
                                                                                                                                  cGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCCGCCACATTAAAAAAGCTCGCTTCGGCGAgg                     >  1:1008604/1‑150 (MQ=255)
                                                                                                                                  cGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGc                     >  6:732373/1‑151 (MQ=255)
                                                                                                                                  cGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGc                     >  5:1817483/1‑151 (MQ=255)
                                                                                                                                   gTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  8:1165323/1‑151 (MQ=255)
                                                                                                                                   gTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  6:962393/1‑151 (MQ=255)
                                                                                                                                    tACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  5:589845/1‑151 (MQ=255)
                                                                                                                                     aCCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  4:283420/1‑151 (MQ=255)
                                                                                                                                        ccAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  4:1821945/1‑151 (MQ=255)
                                                                                                                                        ccAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  5:989793/1‑151 (MQ=255)
                                                                                                                                        ccAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  3:1110969/1‑151 (MQ=255)
                                                                                                                                          aGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  3:649972/1‑151 (MQ=255)
                                                                                                                                          aGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  1:1720400/1‑151 (MQ=255)
                                                                                                                                            ccAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  5:1867879/1‑151 (MQ=255)
                                                                                                                                            ccAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  3:463093/1‑151 (MQ=255)
                                                                                                                                             cAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  7:1750944/1‑151 (MQ=255)
                                                                                                                                              aaTTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  5:618807/1‑151 (MQ=255)
                                                                                                                                               atatATTCAAGCCGC‑GTCACATGCAACAGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        <  1:901576/148‑1 (MQ=21)
                                                                                                                                               aTTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  7:823631/1‑151 (MQ=255)
                                                                                                                                                tttATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg       >  3:808111/1‑151 (MQ=255)
                                                                                                                                                 ttATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  5:1568855/1‑151 (MQ=255)
                                                                                                                                                  tATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTCCCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  2:611653/1‑151 (MQ=255)
                                                                                                                                                  tATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  4:1552366/1‑151 (MQ=255)
                                                                                                                                                   aTTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  8:1309220/1‑151 (MQ=255)
                                                                                                                                                   aTTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  8:720831/1‑151 (MQ=255)
                                                                                                                                                      cAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  3:1514851/1‑150 (MQ=255)
                                                                                                                                                      cAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  4:880879/1‑150 (MQ=255)
                                                                                                                                                      cAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  7:897251/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CGAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184435‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑