Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC23.7% 743.5 / 65.1 365G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (77/200);  new base C (85/1);  total (162/203)
Fisher's exact test for biased strand distribution p-value = 6.01e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447254‑3447552
                                                                                                                                                     |                                                                                                                                                      
       aGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑Tc                                                                                                                                               <  8:1690110/151‑1 (MQ=255)
           cATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑Tccccc                                                                                                                                           >  3:99863/1‑151 (MQ=255)
           cATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑Tcccc                                                                                                                                            <  5:1043683/150‑1 (MQ=255)
            aTGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCACCCC‑Tcccccc                                                                                                                                          >  5:686025/1‑150 (MQ=255)
            aTGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCa                                                                                                                                          <  7:1178340/151‑1 (MQ=255)
              ggcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAg                                                                                                                                         <  5:1145848/150‑1 (MQ=255)
               gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGc                                                                                                                                        >  5:843509/1‑150 (MQ=255)
                cggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCCGcc                                                                                                                                       >  6:342990/1‑149 (MQ=255)
                cggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCa                                                                                                                                       >  1:1006439/1‑150 (MQ=255)
                 ggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCACCat                                                                                                                                      >  5:142214/1‑150 (MQ=255)
                        tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGc                                                                                                                                  <  8:1432558/147‑1 (MQ=255)
                         ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGcaccac                                                                                                                             >  1:1416933/1‑151 (MQ=255)
                               cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATccag                                                                                                                       <  5:239083/151‑1 (MQ=255)
                                    tGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCCCCACATcccgccgcc                                                                                                                  >  7:1575547/1‑151 (MQ=255)
                                    tGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGCTTTCATTCGCCGCTTCGGTGATCACGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑TCCCCAACCAAATGCACCACATccagccgcc                                                                                                                  >  6:786373/1‑151 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATATTCACCACATccagccgcccgc                                                                                                               >  1:81196/1‑151 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCAAATGCCCCACATccacccgccagc                                                                                                               >  3:370680/1‑151 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                        <  7:1819228/151‑1 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCa                                                                                                         <  2:1528673/150‑1 (MQ=255)
                                                 gTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATAtt                                                                                                     <  7:1481847/151‑1 (MQ=255)
                                                    cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGGCCCCCCGCA‑ATGGGCCCCATccccccgcccgcccggccgattacc                                                                                                  >  4:622003/1‑139 (MQ=255)
                                                     aTCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCAc                                                                                                 <  3:1536731/151‑1 (MQ=255)
                                                      tCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCAGCATATGCCCCACATCCCGCCGCCCCCCCTCCATATTacacc                                                                                                >  3:507757/1‑150 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCCGCCGCCCGCCGGCCCTATTCCACgg                                                                                               >  1:519487/1‑151 (MQ=255)
                                                        gCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCACCATATGCCCCACATCCAGCCCCCCCCCGGCCaaaatacaacgc                                                                                              >  5:550255/1‑140 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                             <  2:820169/151‑1 (MQ=255)
                                                          cAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGTCCCACCCGCC‑TCCCCCAGCATAAGGACCACAACCCGCCGCCAGCCGTGCAAATTACACGgccc                                                                                            >  6:1827892/1‑149 (MQ=255)
                                                          cAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCAGCAAATGCACCACATCCACCCGCCAGCCCTCCATATTCCCCGgcgc                                                                                            >  8:801238/1‑151 (MQ=255)
                                                           aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCCCCTATGCCCCACATCCAGCCCCCACCCGTCCATATTCCACGgcgcg                                                                                           >  1:18184/1‑150 (MQ=255)
                                                           aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCACCATATTCACCACAACCAGCCGCCACCCGTCCATATTACACCGCGCt                                                                                           >  8:598494/1‑151 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCACCGCC‑GACCCCACCATATGCACCACAAACCCCCCCCAGCCCTCCAAATTCCACGGCGCGTTCAGccc                                                                                   >  6:1541267/1‑150 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCa                                                                                   <  2:1080183/151‑1 (MQ=255)
                                                                     ccGACAATATACGCTTCCGGCTGGGTTTCTTTCGCGGCTGCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGt                                                                                 <  1:1655112/151‑1 (MQ=255)
                                                                     ccGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCAGCATATGCACCACATCCAGCCCCCAGCCCTCCATATTCCACGGCGCTTTCAGCCact                                                                                 >  2:537104/1‑149 (MQ=255)
                                                                     ccGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCCTATGCACCCCATCCAGCCGCCAGCCGGCCATATTCCACCGCGCTTTCAGCCAGt                                                                                 >  1:1374993/1‑151 (MQ=255)
                                                                      cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATATGCACCACCTCCAGCCCCCCGCCGTGCATATTCCCCCGCGCTTTCAGcccggg                                                                                >  4:367267/1‑147 (MQ=255)
                                                                        aCAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGc                                                                              <  2:1750170/151‑1 (MQ=255)
                                                                         cAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑TCCCCCCCCATAAGCCCCCCCACCCGCCGCCCCCCGGCCATATTCCCCCGCGCGTTCAGcccctgggc                                                                             >  3:293492/1‑144 (MQ=255)
                                                                          aaTATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCACCACATCCACCCGCCCGCCGTCCATATTCCACGGCCCGTTCAGCCAGTGGCgg                                                                            >  4:1290450/1‑151 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCCGCATATGCCCCACAACCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCggcc                                                                          >  6:846716/1‑149 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCCACAAAAGCACCACAAACAGCCCCCAGCCCTCCAAAATACACGGCGCATTAAACCAATGGCCGAc                                                                          >  6:265776/1‑150 (MQ=255)
                                                                               aCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCCCATATTCAACCCATCCCCCCGCCAGCCGGCCATATTCCACGGCGCGTTCAGCCAGTGGGGGACaa                                                                        >  1:394405/1‑150 (MQ=255)
                                                                               aCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCATATTCCCCACATCCCGCCGCCAGCCGGCCCTATTCCACGGCGCTTTGAGCCAGTGGCGGACAAt                                                                       >  5:938811/1‑151 (MQ=255)
                                                                                 gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCCCCATATGCACCACATCCAGCCGCCAGCCGTACATATTCCACCGCGCATTCAGCCAGTGGCGGACAATAc                                                                     >  1:1710165/1‑151 (MQ=255)
                                                                                 gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCACCAAATGCACCACATCCAGCCCCCACCCCTACATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAc                                                                     >  8:201417/1‑151 (MQ=255)
                                                                                  cTTCCGGCTGTGTTTCTTTCGCCGCTGCGCTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCGCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATa                                                                      <  8:674414/149‑1 (MQ=255)
                                                                                  cTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCACCACATCCCGCCGCCCGCCGGCCATATTCCACGGCGCTTTCCGCCAGTGGCGGCCAATACt                                                                    >  5:1323640/1‑151 (MQ=255)
                                                                                    tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTg                                                                   <  3:852047/150‑1 (MQ=255)
                                                                                    tCCGGCTGGGGTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTg                                                                   <  5:1556487/150‑1 (MQ=255)
                                                                                      cggcggGGGTTCTTTCGCCGCTTCGGTGATCCCGGTAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCt                                                                <  3:1594967/151‑1 (MQ=255)
                                                                                      cGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCt                                                                <  8:1558349/151‑1 (MQ=255)
                                                                                      cGGCTGGCTGGCTATCGCCGCTTCGGTGATCCCGGCAACTTGCTGCATATTATTCCGCGCCCCACCCCCC‑TCCCCCCGCATAAGCACCACATCCAGCCGCCAGCCGGCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCt                                                                <  8:1248096/151‑1 (MQ=255)
                                                                                           gggTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTAAGCCAGTGGCGGACAATACTGTCTTcccc                                                           >  4:1384811/1‑151 (MQ=255)
                                                                                           gggTTTCTTTCACCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑TCCCCCAGCATATGCACCACCTCCCGCCGCCCGCCGTCCCTATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCGTcccc                                                           >  8:501090/1‑151 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                      <  7:1123181/148‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                    <  8:1848800/150‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                    <  7:1710617/150‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATa                                                     <  8:1368187/149‑1 (MQ=255)
                                                                                                    tcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaaa                                                   <  6:548995/150‑1 (MQ=255)
                                                                                                     cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAt                                                  <  7:1403495/150‑1 (MQ=255)
                                                                                                     cgccgcTTCGGTGATCCCGGCAACGTGCAGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAt                                                  <  4:968259/150‑1 (MQ=255)
                                                                                                      gccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAttt                                                <  3:1198767/151‑1 (MQ=255)
                                                                                                        cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCCCGGCGCGTTCAGCCAGTGGCGGACCATACTGGCTTCCCCGGGCTTAATttc                                               >  7:1488218/1‑150 (MQ=255)
                                                                                                        cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttca                                              <  6:99441/151‑1 (MQ=255)
                                                                                                         gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑TCCCCCAGCATATTCACCCCAACCCCCCGCCCGCCGTCCCTATTACACCGCGCGTTGAGCCAGTGGCGGCCCAGACAGGCGTGCCCGCGAGAAATttgat                                             >  1:1291964/1‑151 (MQ=255)
                                                                                                          cTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCCGCATATGCACCACCTGCAGCCGCCCGCCGTGCATATTCCACCGCGCTTTGAGCCCGTGGCGGACAATTCTGTGTTCCCCGCGGTAAATttcatt                                            >  6:939434/1‑151 (MQ=255)
                                                                                                            tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattc                                           <  3:1451156/150‑1 (MQ=255)
                                                                                                             cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                          <  6:1119596/150‑1 (MQ=255)
                                                                                                              ggTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACCTCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCAcc                                        >  6:1705337/1‑151 (MQ=255)
                                                                                                               gTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑ACCCCCACCAAATTCAACCCAAACACCCCCCAGCCCGCCAAATTACCCCGCGCATTAAGCCAGTGGCCGCCAATAAAGTGATGCCCGCGATAAAtttaaataaac                                        >  4:1545409/1‑142 (MQ=255)
                                                                                                                ggATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAg                                      <  3:732558/150‑1 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACCAACAGCCGCCACCCGTCAATATTACACCGCCCTTTCAGCCAGGGGCGGCCAATACAGTGGTACCCGCGATAAATTTAATTCACCac                                      >  8:1815550/1‑149 (MQ=255)
                                                                                                                  aTCCCGGCAACGTGCTGGATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                    <  4:1228401/151‑1 (MQ=255)
                                                                                                                  aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGACAATACCGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                    >  3:1770810/1‑151 (MQ=255)
                                                                                                                     ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                 >  2:448477/1‑151 (MQ=255)
                                                                                                                     ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCACCAAATGCCCCACCTCCCGCCCCCAGCCGTCCCTATTACACGGCGCGTTGAGCCAGTGGCGGACCATAATGTATTACCCGCGATAAATTTAATTAAACAGaaatt                                 >  4:1849439/1‑147 (MQ=255)
                                                                                                                         cAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                              >  3:1334355/1‑150 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑TCCCCCAGCATATGCACCACAACCAGCCGCCCGCCGTCCATATTACACGGCCCTTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGATAAATTTAATTCACCAGACATTCCGAc                            >  5:947658/1‑151 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCGAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGAc                            <  7:741915/151‑1 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGAc                            <  1:1055806/151‑1 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACGTTCCgcc                            >  2:202010/1‑149 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCACCATATGCACCACATACAGCCGCCAGCCGTCCATATTACACGGCGCATTAAGCCAGTGGCCGACAATACAGTCTTCCCCGCGATAAAATTCATTCACCAGACtttaccac                            >  4:1766525/1‑146 (MQ=255)
                                                                                                                             gTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCCGCCGCCAGCCGTCCATATTCCCCGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACGTTCCGCCTg                          >  7:1284952/1‑150 (MQ=255)
                                                                                                                             gTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGa                         <  2:1609341/151‑1 (MQ=255)
                                                                                                                              tgctgcATATTATTGCGCGCCCCCCCCGCC‑GGCCCCAGCATATGGCCCCCCTCCAGCCGCCCCCCGGCCATATTACACCGCGCGTTCAGCCCGGGGCGGCCAATACTGTGGTCCCCGCGCTAAATTTCATTACCCAGACGTTCCgccggct                        >  7:869419/1‑145 (MQ=255)
                                                                                                                               gctgcATATTAGTGCTCGCCCGACCGGCC‑TCCCCCAGCATATGCACCACATCCACCGGCCATCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATTCTTTCTTCCCCTCGATAAATTTCATTCACCAGACTTTCCGACTGATa                       <  7:1064983/151‑1 (MQ=255)
                                                                                                                                   cATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATATGCCCCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGCTAATcc                   >  1:1472235/1‑151 (MQ=255)
                                                                                                                                      attattGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGGCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAg                 <  6:1349595/150‑1 (MQ=255)
                                                                                                                                       ttattGCGCGCCCCCCCCGCC‑GCCCCCAGCATATGCCCCACATCCAGCCGCCCGCCGTCCCTATTCCCCGGCGCTTTCAGCCAGTGGCGGGCAATACTGTCTTCCCCGCGATAAATTTGCTTCCCCAGACTTTGCGACTGATAATCCACCt               >  1:117172/1‑151 (MQ=255)
                                                                                                                                         attGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCtt              <  6:733349/150‑1 (MQ=255)
                                                                                                                                         attGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTc             <  6:610756/151‑1 (MQ=255)
                                                                                                                                            gcgcgcCCCACCCGCC‑TCCCCGAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCgtc          <  8:1004533/151‑3 (MQ=255)
                                                                                                                                            gcgcgcCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGc          <  3:1103931/151‑1 (MQ=255)
                                                                                                                                             cgcgcCCCCCCCGCC‑TCCCCCAGCATATGCCCCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTACCCGCGATAAATTTAATTACCCAGACTTTACGACTGATAATCCAGCTTCGGcc         >  2:1212183/1‑150 (MQ=255)
                                                                                                                                               cgcCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAg       <  3:1382581/151‑1 (MQ=255)
                                                                                                                                               cgcCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGGCCCTATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCTATCACCAGACTTTCCGACTGATAATCCAGCTGCGGCAAg       <  4:809319/151‑1 (MQ=255)
                                                                                                                                                  cccACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg    <  4:641127/151‑1 (MQ=255)
                                                                                                                                                   ccACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTgg   >  2:1655289/1‑151 (MQ=255)
                                                                                                                                                    cACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  <  5:315566/151‑1 (MQ=255)
                                                                                                                                                     aCCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  >  7:402008/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447254‑3447552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑