Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 205,979 T→C D189D (GAT→GAC)  yhjG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1205,9790TC99.2% 1848.9 / ‑0.1 491D189D (GAT→GAC) yhjGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (240/241);  total (247/244)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

Only 100 of 497 total aligned reads displayed.

GGACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGT  >  DH1/205831‑206126
                                                                                                                                                    |                                                                                                                                                   
  aCGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCt                                                                                                                                                 >  6:507272/1‑151 (MQ=255)
   cGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCtt                                                                                                                                                <  1:998422/151‑1 (MQ=255)
    gTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTa                                                                                                                                               <  1:1040290/151‑1 (MQ=255)
    gTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGAAAAGCGGATCTCGAGATTTTTGTTGACCCCtt                                                                                                                                                >  6:988048/1‑150 (MQ=255)
    gTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTNNGNCTGGATAATATTCTTTTNGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTa                                                                                                                                               <  4:109872/151‑1 (MQ=255)
       tAATTTTGCCAACGATGTTAACAAAGACGCGAATGCAAAGCGGTGGGCATGGTCGTTTCGGGTGGATAATATTGTTTTCGATCAAGGGCGGATCGGCATTGATGAGAATGTAAGGAAAGGGGATCTGGAGATTTTTGTTGACCCCTTAgg                                                                                                                                             <  8:1571765/150‑1 (MQ=255)
        aaTCTTTCCAACGATGATAACAAAGACGCGAATGCAAAGCAGTCTGCATGGTCGTTTCGGCTTGATAATATTCTTATCGATCAAGGGCGTATCGCCATTGATGACAATGTAATCAAAGCTGATCTGGAGATTTTTGTTGACCCCTTAGGc                                                                                                                                            <  7:83219/150‑1 (MQ=255)
         aTCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCa                                                                                                                                           >  8:346122/1‑150 (MQ=255)
           cTTGCCAACGATGATAACAAAGCCGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGAGTTTTGTTGACCCCTTAGGCCAGc                                                                                                                                        >  2:975004/1‑151 (MQ=255)
           cTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGc                                                                                                                                        >  5:442744/1‑151 (MQ=255)
              gCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCgg                                                                                                                                     >  6:1853086/1‑150 (MQ=255)
              gCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCg                                                                                                                                      <  7:33112/150‑1 (MQ=255)
               ccAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCt                                                                                                                                    <  2:1591407/151‑1 (MQ=255)
               ccAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGAGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCt                                                                                                                                    >  3:511890/1‑151 (MQ=255)
                cAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTg                                                                                                                                   >  3:788556/1‑151 (MQ=255)
                  aCGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGcc                                                                                                                                 >  5:606840/1‑151 (MQ=255)
                   cGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCg                                                                                                                                >  8:1330757/1‑151 (MQ=255)
                      tgatAACAAAGACGCGAATGCAAAGCCCTCGGCATCGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGCCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGAACCCTTAGGCCAGCCGCTCCCGtgc                                                                                                                             >  4:847518/1‑149 (MQ=255)
                             aaaGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGa                                                                                                                        >  5:325212/1‑149 (MQ=255)
                                 ccgcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTAc                                                                                                                  <  7:736809/150‑1 (MQ=255)
                                  cgcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACt                                                                                                                 <  1:529454/151‑1 (MQ=255)
                                    cgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTgg                                                                                                               <  3:24540/151‑1 (MQ=255)
                                     gAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGAGCAAGGGCGGATAGCCATTGATGACAAAGTAAGCAACGCGGATCTGGAGATTTTTGTTGACCCCGTAGGCAAGCCGCTGCGGTTCAGCGAAGTTAGTgg                                                                                                               >  7:1160642/1‑150 (MQ=255)
                                      aaTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGAt                                                                                                             <  7:1901718/151‑1 (MQ=255)
                                      aaTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGAt                                                                                                             <  6:606475/151‑1 (MQ=255)
                                         gCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCg                                                                                                           >  2:430193/1‑150 (MQ=255)
                                          cAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGa                                                                                                          >  8:1491524/1‑150 (MQ=255)
                                          cAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGa                                                                                                          >  4:229043/1‑150 (MQ=255)
                                           aaaGCCGTCGTCATTGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCATCGAAGTTACTGGATCGaaa                                                                                                        <  3:1621099/151‑1 (MQ=255)
                                           aaaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGaaa                                                                                                        <  8:578807/151‑1 (MQ=255)
                                           aaaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGaa                                                                                                         >  1:1555174/1‑150 (MQ=255)
                                            aaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAg                                                                                                       <  2:692917/151‑1 (MQ=255)
                                             aGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAgg                                                                                                      <  2:789751/151‑1 (MQ=255)
                                               ccGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGt                                                                                                     >  6:341840/1‑150 (MQ=255)
                                                cGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACATAGTTAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTaa                                                                                                   <  8:837668/151‑1 (MQ=255)
                                                cGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATAGAAAGGTa                                                                                                    >  5:648888/1‑150 (MQ=255)
                                                 gTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTaaa                                                                                                  <  1:582230/151‑1 (MQ=255)
                                                  tCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTaa                                                                                                   >  2:400602/1‑149 (MQ=255)
                                                  tCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGAACAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTaa                                                                                                   >  5:860732/1‑149 (MQ=255)
                                                   cGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAg                                                                                                 <  6:793285/150‑1 (MQ=255)
                                                   cGGCATGGGCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCAGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGc                                                                                                <  4:1628202/151‑1 (MQ=255)
                                                     gCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGCCCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                              >  6:239624/1‑151 (MQ=255)
                                                     gCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                              >  1:1467521/1‑151 (MQ=255)
                                                       aTGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGAt                                                                                            >  4:90063/1‑151 (MQ=255)
                                                       aTGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGAt                                                                                            >  3:146084/1‑151 (MQ=255)
                                                       aTGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGAt                                                                                            >  3:1330859/1‑151 (MQ=255)
                                                       aTGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCAGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTAATGGATCGAAAGGTAAAGCGGAt                                                                                            >  7:1625014/1‑151 (MQ=255)
                                                         ggTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGTATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                          <  5:1104351/151‑1 (MQ=255)
                                                          gTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                          >  7:172304/1‑150 (MQ=255)
                                                            cGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                          >  7:67126/1‑148 (MQ=255)
                                                            cGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAg                                                                                        <  1:743718/150‑1 (MQ=255)
                                                             gTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGa                                                                                       >  5:1385020/1‑150 (MQ=255)
                                                                 cGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAg                                                                                   <  8:2044/150‑1 (MQ=255)
                                                                 cGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAg                                                                                   <  2:1076962/150‑1 (MQ=255)
                                                                  ggCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGt                                                                                 >  8:1712446/1‑151 (MQ=255)
                                                                    cTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTgg                                                                               >  4:1344365/1‑151 (MQ=255)
                                                                     tGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTggg                                                                              >  2:1175526/1‑151 (MQ=255)
                                                                        ataataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCg                                                                            >  8:445943/1‑150 (MQ=255)
                                                                        ataataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGa                                                                           <  5:1790048/151‑1 (MQ=255)
                                                                             atTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACg                                                                      <  5:229561/151‑1 (MQ=255)
                                                                              ttCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGt                                                                     >  7:1473234/1‑151 (MQ=255)
                                                                               tCTTTTCTATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGt                                                                     <  2:618491/150‑1 (MQ=255)
                                                                                cTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGttt                                                                   >  7:1352942/1‑151 (MQ=255)
                                                                                   ttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGttttt                                                                 <  3:1750828/150‑1 (MQ=255)
                                                                                    tCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTgg                                                               >  7:999845/1‑151 (MQ=255)
                                                                                     cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTgg                                                               <  2:529487/150‑1 (MQ=255)
                                                                                     cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTgg                                                               <  3:123148/150‑1 (MQ=255)
                                                                                     cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGc                                                              <  1:240643/151‑1 (MQ=255)
                                                                                       aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                            >  7:1183092/1‑151 (MQ=255)
                                                                                         cAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTg                                                           >  3:1085695/1‑150 (MQ=255)
                                                                                         cAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGCATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCCGATAAAGAAAAGCTGGGCGATTACGTTATTCGCCTg                                                           >  1:809758/1‑150 (MQ=255)
                                                                                           aGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAg                                                        <  6:1380364/151‑1 (MQ=255)
                                                                                                ggATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCa                                                   <  1:1373203/151‑1 (MQ=255)
                                                                                                 gATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGgcgc                                                    >  2:1346499/1‑149 (MQ=255)
                                                                                                 gATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGA‑TACGTTTTTGGCCTGAAGGCGCAgg                                                 >  3:88530/1‑151 (MQ=255)
                                                                                                      ccATTGATGACAAAGTAAGCAAAGGGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACg                                             >  1:1619934/1‑151 (MQ=255)
                                                                                                       cATTGATCACAAAGTAACCAAAGCCGAAATGGAGATTATTCTTGACCACTTAGGCAAGCCGCTGCAGTTCAGAGAAGTTACTGGATCGAAAGGTAAAGCGCATAAAGAAAACCTGGGCGATTACGATTTTGGCCTGAAGGCGCACGGACg                                             >  2:833413/1‑150 (MQ=255)
                                                                                                        aTTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGGAGGGACGat                                           >  6:1224768/1‑151 (MQ=255)
                                                                                                        aTTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGat                                           >  3:420680/1‑151 (MQ=255)
                                                                                                           gatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGatat                                         <  7:429257/150‑1 (MQ=255)
                                                                                                                cAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTAATGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGt                                   >  8:1774518/1‑151 (MQ=255)
                                                                                                                 aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTg                                  >  8:119107/1‑151 (MQ=255)
                                                                                                                  aaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATGAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGa                                 <  2:202778/151‑1 (MQ=255)
                                                                                                                  aaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGTCGCAGGGACGATATAACGGTg                                  >  3:674816/1‑150 (MQ=255)
                                                                                                                     tAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAAcc                              >  7:940743/1‑151 (MQ=255)
                                                                                                                      aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCg                             <  5:1689426/151‑1 (MQ=255)
                                                                                                                      aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCg                             >  5:1535769/1‑151 (MQ=255)
                                                                                                                         cAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                          >  4:467550/1‑151 (MQ=255)
                                                                                                                         cAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCCCTGCCCTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACCTTTTTCGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                          >  6:16903/1‑151 (MQ=255)
                                                                                                                          aaaGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                          >  4:1728617/1‑150 (MQ=255)
                                                                                                                            aGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACg                       <  6:1605283/151‑1 (MQ=255)
                                                                                                                              cGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACggg                     <  4:712615/151‑1 (MQ=255)
                                                                                                                                  tCTGGAGATTTTTGTTGAGCCCTTAGGCAAGCCGCTGCCGTTCAGGGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTTGGCGATAACGTTTTTGGCCTGAAGGCGCAGGGACCATATAACGGTGAACCGCTCACGGCTACg                 >  7:1094026/1‑151 (MQ=255)
                                                                                                                                    tGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACggg               >  3:966911/1‑151 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACggg               >  6:899891/1‑150 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACgg                <  4:1874399/149‑1 (MQ=255)
                                                                                                                                      gagaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGa              >  5:973389/1‑150 (MQ=255)
                                                                                                                                       agaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaa             >  1:1217931/1‑150 (MQ=255)
                                                                                                                                        gaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaaa            >  6:1753322/1‑150 (MQ=255)
                                                                                                                                               gttgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGc     <  1:1691905/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGT  >  DH1/205831‑206126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑