Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,140,096 T→C E180G (GAA→GGA)  purR ← PurR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,140,0960TC99.1% 1673.2 / 0.7 436E180G (GAA→GGA) purRPurR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (219/213);  total (220/216)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 436 total aligned reads displayed.

GCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCC  >  DH1/2139949‑2140242
                                                                                                                                                   |                                                                                                                                                  
 cgcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgcg                                                                                                                                                <  4:388040/151‑1 (MQ=255)
 cgcgATAACCGGATTCAGGTTCAAAGGCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgcg                                                                                                                                                <  3:655661/151‑1 (MQ=255)
  gcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTGACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCgg                                                                                                                                               >  7:1780211/1‑151 (MQ=255)
   cgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGt                                                                                                                                              >  2:766475/1‑151 (MQ=255)
    gATAACCGGATTCAGGTTCAAAGTCAACCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTg                                                                                                                                             >  7:696861/1‑151 (MQ=255)
     aTAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTg                                                                                                                                             >  5:1515753/1‑150 (MQ=255)
     aTAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTg                                                                                                                                             >  8:598763/1‑150 (MQ=255)
         ccGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcgc                                                                                                                                        <  3:1577798/151‑1 (MQ=255)
         ccGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcgc                                                                                                                                        <  4:1445689/151‑1 (MQ=255)
         ccGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcgc                                                                                                                                        <  4:43474/151‑1 (MQ=255)
          cGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcgcg                                                                                                                                       <  4:13356/151‑1 (MQ=255)
           ggATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGt                                                                                                                                      <  8:1039852/151‑1 (MQ=255)
             aTTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTc                                                                                                                                    <  7:1604922/151‑1 (MQ=255)
                      cAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGAt                                                                                                                            >  3:738899/1‑150 (MQ=255)
                       aaaGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATa                                                                                                                           >  6:350355/1‑150 (MQ=255)
                        aaGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAAc                                                                                                                         >  5:1116842/1‑151 (MQ=255)
                           tCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGcc                                                                                                                      >  2:531732/1‑151 (MQ=255)
                            cACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGccc                                                                                                                     >  8:946362/1‑151 (MQ=255)
                              cccTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCgg                                                                                                                   >  2:1097747/1‑151 (MQ=255)
                                  gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCAt                                                                                                               <  6:1241364/151‑1 (MQ=255)
                                  gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCAt                                                                                                               <  8:679963/151‑1 (MQ=255)
                                  gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTACATAAAACCGGCAAGGCGGCGTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGcc                                                                                                                 <  4:965331/149‑1 (MQ=255)
                                  gCACAATCCAGCTTTCCGGCACCTTGATCAGCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGcc                                                                                                                 <  3:1232645/149‑1 (MQ=255)
                                   cacaATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATg                                                                                                              >  2:296969/1‑151 (MQ=255)
                                      aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGt                                                                                                             >  7:154437/1‑149 (MQ=255)
                                      aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAg                                                                                                           <  4:605157/151‑1 (MQ=255)
                                         ccAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgccg                                                                                                        <  4:1224487/151‑1 (MQ=255)
                                          cAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgccgc                                                                                                       >  2:425975/1‑151 (MQ=255)
                                          cAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgccgc                                                                                                       >  3:794729/1‑151 (MQ=255)
                                            gCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCt                                                                                                     >  4:1072962/1‑151 (MQ=255)
                                               ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   <  2:1424857/150‑1 (MQ=255)
                                               ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCg                                                                                                  >  7:1154951/1‑151 (MQ=255)
                                               ttCCGGCACCTTGATCATCGCTGCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCtt                                                                                                    <  5:893803/149‑1 (MQ=255)
                                                tCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCg                                                                                                  <  3:1494384/150‑1 (MQ=255)
                                                tCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCg                                                                                                  >  5:1658707/1‑150 (MQ=255)
                                                  cGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAc                                                                                               <  5:1228481/151‑1 (MQ=255)
                                                   ggCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcg                                                                                              <  2:1126010/151‑1 (MQ=255)
                                                    gCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGCTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcgc                                                                                             >  7:734899/1‑151 (MQ=255)
                                                       ccTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  6:1294388/1‑151 (MQ=255)
                                                        cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  6:805727/1‑150 (MQ=255)
                                                        cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  1:1412446/1‑150 (MQ=255)
                                                        cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  1:982898/1‑150 (MQ=255)
                                                        cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGAACGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGCACGCGtt                                                                                          >  3:1097648/1‑150 (MQ=255)
                                                         ttGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGCTCTTACGTTCCAGCGGGCCCGGCATCACGCCGATTCCGCGGTCACCGCCTTCAATCAGATAACGCCCCCCCATCTAGCCGCCTACGAAcgccata                                                                                         >  6:245149/1‑146 (MQ=255)
                                                          tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTa                                                                                         <  6:60207/149‑1 (MQ=255)
                                                          tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATc                                                                                       >  7:1698247/1‑151 (MQ=255)
                                                            atcctcGCTTCTTCCAGCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGGGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCaa                                                                                     <  8:834090/151‑1 (MQ=255)
                                                            atcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGAGGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCaa                                                                                     <  8:1452930/151‑1 (MQ=255)
                                                               atcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATgc                                                                                  >  8:1867477/1‑150 (MQ=255)
                                                                 cGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAc                                                                                 <  1:439910/150‑1 (MQ=255)
                                                                  gCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCg                                                                               <  1:614408/151‑1 (MQ=255)
                                                                    ttcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGc                                                                              >  7:535794/1‑150 (MQ=255)
                                                                     tcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            >  2:1641715/1‑151 (MQ=255)
                                                                     gcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            <  5:1842655/150‑1 (MQ=255)
                                                                        tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            <  4:1826704/148‑1 (MQ=255)
                                                                        tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCgg                                                                         >  6:1535198/1‑151 (MQ=255)
                                                                         ccATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGt                                                                        >  6:1037338/1‑151 (MQ=255)
                                                                          cATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTg                                                                       <  7:204376/151‑1 (MQ=255)
                                                                            tCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGa                                                                      >  2:232812/1‑150 (MQ=255)
                                                                             cGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGaa                                                                     <  2:632893/150‑1 (MQ=255)
                                                                             cGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGa                                                                      >  4:1609975/1‑149 (MQ=255)
                                                                              gCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGt                                                                   <  6:1485639/151‑1 (MQ=255)
                                                                              gCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGt                                                                   >  1:1582699/1‑151 (MQ=255)
                                                                                  tCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCTGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGc                                                               >  3:1349126/1‑151 (MQ=255)
                                                                                  tCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCa                                                                 <  3:17587/149‑1 (MQ=255)
                                                                                  tCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAg                                                                <  1:1035658/150‑1 (MQ=255)
                                                                                  tCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGc                                                               >  7:142638/1‑151 (MQ=255)
                                                                                   cATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCt                                                              <  8:1302494/151‑1 (MQ=255)
                                                                                      aaaaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCttt                                                            <  1:1275805/150‑1 (MQ=255)
                                                                                      aaaaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTACCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCtttt                                                           >  7:883254/1‑151 (MQ=255)
                                                                                        aaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGc                                                         <  2:1283588/151‑1 (MQ=255)
                                                                                              cAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCAcc                                                   >  1:1708707/1‑151 (MQ=255)
                                                                                               aaGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCAccc                                                  >  3:427329/1‑151 (MQ=255)
                                                                                                  gcggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAg                                               <  1:530925/151‑1 (MQ=255)
                                                                                                    ggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTc                                             >  1:1078482/1‑151 (MQ=255)
                                                                                                    ggcCTGCGCAGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACAGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTc                                             >  7:185862/1‑151 (MQ=255)
                                                                                                     gcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCCCACCAGTcc                                            >  5:1436491/1‑151 (MQ=255)
                                                                                                     gcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTcc                                            <  7:1340044/151‑1 (MQ=255)
                                                                                                           gcCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGacc                                      <  5:767019/151‑1 (MQ=255)
                                                                                                             cGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGAATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccac                                    >  6:1708762/1‑151 (MQ=255)
                                                                                                               gtgtTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccacc                                   >  5:213523/1‑150 (MQ=255)
                                                                                                                tgtTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCAt                                 >  5:665164/1‑151 (MQ=255)
                                                                                                                   tACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTgg                              >  5:827585/1‑151 (MQ=255)
                                                                                                                      gTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATCACGCCCGGCCATGTAGGCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGat                           <  6:919832/151‑1 (MQ=255)
                                                                                                                         ccAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATg                        <  1:184696/151‑1 (MQ=255)
                                                                                                                          cAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATgg                       >  7:491994/1‑151 (MQ=255)
                                                                                                                           aGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGc                      >  1:917014/1‑151 (MQ=255)
                                                                                                                              gggCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGTGAt                   >  8:546574/1‑151 (MQ=255)
                                                                                                                                 ccGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATAct                >  3:1431591/1‑151 (MQ=255)
                                                                                                                                 ccGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATAct                <  7:377980/151‑1 (MQ=255)
                                                                                                                                   ggggATGACGCCGATTCGGCGGTGACCGCGTTCATTCAGATAACGCCGGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTAACCCCAGTGCATGACCACCATTGGGATATGGCGATActct              <  2:1404823/151‑1 (MQ=255)
                                                                                                                                   ggggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTAGGATATGGCGATActct              >  4:101945/1‑151 (MQ=255)
                                                                                                                                    gggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCtt             <  2:1202868/151‑1 (MQ=255)
                                                                                                                                      gATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCGTGTAGTCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTcc           <  8:1572622/151‑1 (MQ=255)
                                                                                                                                       aTGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCa          <  1:766166/151‑1 (MQ=255)
                                                                                                                                       aTGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCa          <  4:823351/151‑1 (MQ=255)
                                                                                                                                         gACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAg         <  5:361175/150‑1 (MQ=255)
                                                                                                                                           cGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCCCCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGc        >  5:474382/1‑149 (MQ=255)
                                                                                                                                            gCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATc     <  3:1860388/151‑1 (MQ=255)
                                                                                                                                            gCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATc     >  1:19654/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCC  >  DH1/2139949‑2140242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑