Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,773,985 (A)8→7 coding (440/1119 nt) wbbK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,773,9780A.96.5% 2121.8 / 67.7 489coding (433/1119 nt)wbbKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (10/7);  new base . (229/243);  total (239/250)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 521 total aligned reads displayed.

ATTACGGCCAATGTCGTCACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTAAAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCAATTTAA  >  DH1/1773828‑1774123
                                                                                                                                                      |                                                                                                                                                 
   aCGGCCAATGTCGTCACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTaaa                                                                                                                                                 >  3:1096424/1‑150 (MQ=255)
    cGGCCAATGTCGTCACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTaaaaa                                                                                                                                               <  6:1212554/151‑1 (MQ=255)
     ggCCAATGTCGTCACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTaaaaaa                                                                                                                                              <  1:1006153/151‑1 (MQ=255)
            gtcgtcACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTAAAAAAAATACTg                                                                                                                                       <  2:141894/151‑1 (MQ=255)
            gtcgtcACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTg                                                                                                                                       >  8:124291/1‑150 (MQ=255)
            gtcgtcACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGc                                                                                                                                      >  2:849459/1‑151 (MQ=255)
            gtcgtaACTAAAAAAATATATGTGTATTGTCTTAACCCTGCACCTTTTTATAAAGTAATTTTATTCCGTTAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTg                                                                                                                                       <  2:769535/150‑1 (MQ=255)
               gtcACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAgt                                                                                                                                   <  7:1794942/151‑1 (MQ=255)
               gtcACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAgt                                                                                                                                   >  6:1752748/1‑151 (MQ=255)
                 cACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAgtg                                                                                                                                  <  6:1076276/150‑1 (MQ=255)
                    tAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGtgtttg                                                                                                                              >  2:1735240/1‑151 (MQ=255)
                     aaaaaaaGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGtgtttgt                                                                                                                             <  3:1483461/151‑1 (MQ=255)
                           aGATATGTGTATTGGCATACCCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTcaaca                                                                                                                       <  2:1945015/151‑1 (MQ=255)
                             atatGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTcaacaa                                                                                                                      <  4:281661/150‑1 (MQ=255)
                             atatGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAAt                                                                                                                     >  1:560413/1‑151 (MQ=255)
                                 gtgtATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCt                                                                                                                  <  3:1542800/150‑1 (MQ=255)
                                     aTTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGAt                                                                                                              <  7:311247/150‑1 (MQ=255)
                                     aTTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATg                                                                                                             <  3:1138701/151‑1 (MQ=255)
                                      ttttCATAACCCTGAAGCTTTTTTTAATGGAATTTTATTCCGTGAAATTCTTATGGAGCCTATCTTTTTCTTATTTAAATTGCTATACTTGCTGATATATAAAATAAAGATTAAAAAAAATACAGCAGTGTTTGTTCAACAATTCTGGAt                                                                                                              <  3:928828/147‑1 (MQ=255)
                                        gTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCCGGATgaa                                                                                                           <  1:1617946/150‑1 (MQ=255)
                                          cATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATgaaaga                                                                                                        <  1:409731/151‑1 (MQ=255)
                                             aaCCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATgaaagaaa                                                                                                      >  4:1485433/1‑150 (MQ=255)
                                             aaCCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGaaaa                                                                                                     <  8:450063/151‑1 (MQ=255)
                                              aCCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGaaaaa                                                                                                    <  6:1271683/151‑1 (MQ=255)
                                              aCCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGaaaa                                                                                                     >  4:1689600/1‑150 (MQ=255)
                                                ccTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCTACAATTCTGGATGAAAGAAAAAtt                                                                                                  >  8:565806/1‑151 (MQ=255)
                                                ccTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAAtt                                                                                                  >  1:1125787/1‑151 (MQ=255)
                                                   gCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTAt                                                                                               >  8:1506105/1‑151 (MQ=255)
                                                    cACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATc                                                                                              <  4:1329886/151‑1 (MQ=255)
                                                    cACCTTTTTATAAAGGAATTTTAATCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATc                                                                                              <  4:36201/151‑1 (MQ=255)
                                                        tttttATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTCTATGTTCAACAATACTGGATGAAAGAAAAATTTATCa                                                                                             >  6:201529/1‑148 (MQ=255)
                                                           ttATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGaaa                                                                                        <  5:1965970/150‑1 (MQ=255)
                                                           ttATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAat                                                                                       >  4:820682/1‑151 (MQ=255)
                                                             ataAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAatat                                                                                     <  3:1325358/151‑1 (MQ=255)
                                                             ataAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAata                                                                                      <  1:85743/150‑1 (MQ=255)
                                                                aaGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCt                                                                                  <  5:135270/151‑1 (MQ=255)
                                                                  ggAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCtat                                                                                >  7:212479/1‑151 (MQ=255)
                                                                           ttCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAcat                                                                       >  2:1840339/1‑151 (MQ=255)
                                                                           ttCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAcat                                                                       >  2:1532273/1‑151 (MQ=255)
                                                                           ttCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAca                                                                        >  6:1538979/1‑150 (MQ=255)
                                                                            tCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAcatc                                                                      >  7:1100170/1‑151 (MQ=255)
                                                                            cccGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAcatc                                                                      <  4:656273/150‑1 (MQ=255)
                                                                             ccGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGTCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAcatc                                                                      <  3:1777329/150‑1 (MQ=255)
                                                                             ccGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAAcatc                                                                      >  4:903032/1‑150 (MQ=255)
                                                                                 gAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTg                                                                  <  8:1442813/150‑1 (MQ=255)
                                                                                 gAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGt                                                                 >  2:1242618/1‑151 (MQ=255)
                                                                                 gAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGt                                                                 >  4:566047/1‑151 (MQ=255)
                                                                                   aaTTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTc                                                                >  2:65934/1‑150 (MQ=255)
                                                                                    aTTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTc                                                                >  6:447724/1‑149 (MQ=255)
                                                                                     ttCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGt                                                             >  1:152538/1‑151 (MQ=255)
                                                                                        ttATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCgg                                                          >  5:501317/1‑151 (MQ=255)
                                                                                         tATGGAGCCTAGCTTTTTCTTATTTAAAATGCTCTACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCgg                                                          <  2:1120362/150‑1 (MQ=255)
                                                                                         tATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCg                                                           <  3:785269/149‑1 (MQ=255)
                                                                                          aTGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGcc                                                        >  8:1978089/1‑151 (MQ=255)
                                                                                          aTGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGcc                                                        >  5:935010/1‑151 (MQ=255)
                                                                                          aTGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGc                                                         <  1:1729246/150‑1 (MQ=255)
                                                                                           ttgattcTATCTTTTTCTTATTTAAAATGGTATAATGGCTGATATATAAAATTATCATT‑AAAAAAATTCTGCAGTGTTTGTTCAACAATTCTGGATGTAAGAAAAATTTATCAAGAAATATTCTATAAATAACATGATTGTCAGTCGGcc                                                        <  6:1643780/144‑1 (MQ=255)
                                                                                           tGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGcc                                                        >  5:601216/1‑150 (MQ=255)
                                                                                            ggAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTAAAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGcc                                                        <  7:1236541/150‑1 (MQ=255)
                                                                                               gCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaaa                                                   <  8:168690/151‑1 (MQ=255)
                                                                                                ccTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTAAAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaa                                                    >  5:730386/1‑150 (MQ=255)
                                                                                                ccTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaaat                                                  >  8:1058262/1‑151 (MQ=255)
                                                                                                   aGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaaattaa                                               <  3:1923711/151‑1 (MQ=255)
                                                                                                   aGCTTTATCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaaatta                                                <  1:1890817/150‑1 (MQ=255)
                                                                                                      tttttCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaaattaaat                                             <  8:12973/150‑1 (MQ=255)
                                                                                                       ttttCTTATTTAAAAATCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAACAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGaaattaaatt                                            <  4:1178490/150‑1 (MQ=255)
                                                                                                         ttCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATc                                         >  2:905664/1‑151 (MQ=255)
                                                                                                          tCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATc                                         <  6:734096/150‑1 (MQ=255)
                                                                                                             tATTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGAt                                     >  5:1697559/1‑151 (MQ=255)
                                                                                                              aTTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGAt                                     <  7:1423872/150‑1 (MQ=255)
                                                                                                              aTTTAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATa                                    <  6:1781342/151‑1 (MQ=255)
                                                                                                                 tAAAATGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCACTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATaaa                                  >  3:488693/1‑150 (MQ=255)
                                                                                                                  aaaaTGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATaaaa                                 <  7:441444/150‑1 (MQ=255)
                                                                                                                  aaaaTGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATaaaa                                 <  1:965790/150‑1 (MQ=255)
                                                                                                                  aaaaTGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATaaa                                  >  1:714131/1‑149 (MQ=255)
                                                                                                                   aaaTGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGc                               >  2:1018065/1‑151 (MQ=255)
                                                                                                                   aaaTGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGc                               <  2:567221/151‑1 (MQ=255)
                                                                                                                    aaTGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGcc                              >  2:463209/1‑151 (MQ=255)
                                                                                                                      tGCTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCa                             >  6:634714/1‑150 (MQ=255)
                                                                                                                        cTATACGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACt                          >  2:250384/1‑151 (MQ=255)
                                                                                                                         tataCGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCGAGAAATTAAATTATCTGATAAAAGCCAACtt                         >  8:1748784/1‑151 (MQ=255)
                                                                                                                          ataCGGGCTTATATATAAAATAAACATTAAAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACt                          <  8:1001051/150‑1 (MQ=255)
                                                                                                                          ataCGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGTATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACt                          <  7:1613957/149‑1 (MQ=255)
                                                                                                                          ataCGGGCTGATACATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACtt                         <  2:401118/150‑1 (MQ=255)
                                                                                                                           taCGGGCTGATATATAAAATAAACA‑TAAAAAAAATACTGCAGTGTTTGTTCAACAATTATCGATGAAAGAAAAATTTATAAAGAAATATTCTATAAATAACATCATTGTCAGTGGGCCAGAAATTAAATTATCTGATAAAAGACAACtaa                        >  2:1673604/1‑148 (MQ=255)
                                                                                                                            aCGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTAc                       <  3:345261/150‑1 (MQ=255)
                                                                                                                             cGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACtg                     >  8:758753/1‑151 (MQ=255)
                                                                                                                             cGGGCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACtg                     >  5:339574/1‑151 (MQ=255)
                                                                                                                              gggCTGATATATAAAATAAACATTAAAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACtg                     >  4:962988/1‑151 (MQ=255)
                                                                                                                                gCTGATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCGACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACtgatg                  >  8:868519/1‑151 (MQ=255)
                                                                                                                                   gATATATGAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACtgatgat                <  3:593425/150‑1 (MQ=255)
                                                                                                                                   gATATATAAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGTATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACtgatgat                <  7:125887/150‑1 (MQ=255)
                                                                                                                                     tatataAAATAAACATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTgatgatgat             >  1:1344058/1‑151 (MQ=255)
                                                                                                                                           aaataaaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCaa       <  7:891180/151‑1 (MQ=255)
                                                                                                                                           aaataaaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCa        >  5:866019/1‑150 (MQ=255)
                                                                                                                                           aaataaaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCa        >  5:80677/1‑150 (MQ=255)
                                                                                                                                           aaataaaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTAGGCCAGAAATTAAATTATCTGATAAAAGCCAAATTACTGATGATGATTCTCa        >  6:991387/1‑150 (MQ=255)
                                                                                                                                              taaaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCACCTTACTGATGATGATTCTAAAttt    >  4:1791527/1‑151 (MQ=255)
                                                                                                                                              taaaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCAAttt    >  6:859592/1‑151 (MQ=255)
                                                                                                                                                aaCATT‑AAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCAATTTaa  <  5:1769727/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
ATTACGGCCAATGTCGTCACTAAAAAAAGATATGTGTATTGTCATAACCCTGCACCTTTTTATAAAGGAATTTTATTCCGTGAAATTCTTATGGAGCCTAGCTTTTTCTTATTTAAAATGCTATACGGGCTGATATATAAAATAAACATTAAAAAAAATACTGCAGTGTTTGTTCAACAATTCTGGATGAAAGAAAAATTTATCAAGAAATATTCTATAAATAACATCATTGTCAGTCGGCCAGAAATTAAATTATCTGATAAAAGCCAACTTACTGATGATGATTCTCAATTTAA  >  DH1/1773828‑1774123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑