Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 839,095 Δ1 bp coding (1001/1434 nt) bglA ← 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1839,0940T.98.8% 2217.2 / 18.5 488coding (1002/1434 nt)bglA6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base . (227/253);  total (232/256)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 504 total aligned reads displayed.

CACTTTGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCTTCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGACGCTC  >  DH1/838944‑839240
                                                                                                                                                      |                                                                                                                                                  
cACTTTGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCt                                                                                                                                                    >  2:111112/1‑151 (MQ=255)
 acctgggcGTAAGCGCCAAAACCGGCTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCACAGGGCATCGCGCAGACCTACTGGATCAATCTGCCAGCCCCCGTCAGACGCTTTAACATACGGGGGTGGTACGCCGCCtc                                                                                                                                                   <  2:725773/144‑2 (MQ=255)
 aCTTTGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCtc                                                                                                                                                   <  4:1369073/151‑2 (MQ=255)
   tttGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCtca                                                                                                                                                  >  6:1053886/1‑148 (MQ=255)
   tttGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCtca                                                                                                                                                  >  2:1411167/1‑148 (MQ=255)
    ttGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCAAAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCtca                                                                                                                                                  <  3:760014/149‑3 (MQ=255)
     tGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCCGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCtcaaa                                                                                                                                                >  3:1877383/1‑146 (MQ=255)
      gTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCaaaa                                                                                                                                              <  1:738362/150‑1 (MQ=255)
        cGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCa                                                                                                                                           <  7:1249775/151‑1 (MQ=255)
        cGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCa                                                                                                                                           <  7:355616/151‑1 (MQ=255)
        cGTAAGCGCCAAAACCGTTATCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCa                                                                                                                                           <  1:1674850/151‑1 (MQ=255)
         gTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCag                                                                                                                                          <  8:1687453/151‑1 (MQ=255)
           aaccgcCAAAACCGTTTTCGACAATAAACAGCGCCCACTCAAAACGCTCATACAGTTCCCAAACTTCATAACGAAGACCTACTCGATCAATATCCCACCCCCACACACATGCTATAACATAAGCCTAAGGAACCCT‑CCCTCAAAACCaga                                                                                                                                         >  6:381314/4‑150 (MQ=255)
            aGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCag                                                                                                                                          >  2:572327/1‑148 (MQ=255)
             gcgcCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCag                                                                                                                                          >  6:698846/1‑147 (MQ=255)
                 cAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGatcgca                                                                                                                                  <  4:841880/151‑1 (MQ=255)
                 cAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGatcgca                                                                                                                                  <  7:1204983/151‑1 (MQ=255)
                    aaCCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGatcacatcg                                                                                                                               >  3:1205909/1‑151 (MQ=255)
                      ccGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGcc                                                                                                                             >  5:855746/1‑151 (MQ=255)
                       cGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCg                                                                                                                            <  3:130537/151‑1 (MQ=255)
                        gTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCgg                                                                                                                           >  8:55920/1‑151 (MQ=255)
                          tttCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTg                                                                                                                         >  7:1670742/1‑151 (MQ=255)
                           ttCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTg                                                                                                                         <  4:249342/150‑1 (MQ=255)
                             cGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTgccg                                                                                                                      <  8:1420240/151‑1 (MQ=255)
                             cGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTgccg                                                                                                                      <  5:1764202/151‑1 (MQ=255)
                                    aaaCAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCg                                                                                                                <  7:1357728/150‑1 (MQ=255)
                                     aaCAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGc                                                                                                              <  5:956136/151‑1 (MQ=255)
                                        aGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACCCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCtt                                                                                                           >  6:1932477/1‑151 (MQ=255)
                                              ctctGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGc                                                                                                     >  6:1987421/1‑151 (MQ=255)
                                                  gATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCa                                                                                                    <  6:1348527/148‑1 (MQ=255)
                                                   aTAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTgg                                                                                                >  6:625315/1‑151 (MQ=255)
                                                     aaCGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTc                                                                                              <  6:1228022/151‑1 (MQ=255)
                                                     aaCGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTc                                                                                              <  6:871843/151‑1 (MQ=255)
                                                     aaCGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTCGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGt                                                                                               <  3:1748662/150‑1 (MQ=255)
                                                        gCTCATACAGTTCGCAAAGTGCATCGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATg                                                                                           <  4:1843949/151‑1 (MQ=255)
                                                        gCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATg                                                                                           <  1:272697/151‑1 (MQ=255)
                                                        gCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATg                                                                                           <  2:128887/151‑1 (MQ=255)
                                                         cTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGt                                                                                          >  8:1849906/1‑151 (MQ=255)
                                                           cATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTa                                                                                         >  2:258124/1‑150 (MQ=255)
                                                            aTACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAAt                                                                                       <  7:1810057/151‑1 (MQ=255)
                                                            aTACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTGTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAAt                                                                                       >  3:1001610/1‑151 (MQ=255)
                                                              aCAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCTTCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAAt                                                                                       >  6:7951/1‑150 (MQ=255)
                                                                aGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCt                                                                                   <  2:610963/151‑1 (MQ=255)
                                                                aGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCt                                                                                   <  4:344289/151‑1 (MQ=255)
                                                                 gTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTg                                                                                  <  6:1631068/151‑1 (MQ=255)
                                                                   tCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGa                                                                                 <  1:561468/150‑1 (MQ=255)
                                                                   tCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGa                                                                                 <  1:940648/150‑1 (MQ=255)
                                                                    cGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGACTTCACTGCATTGGTCATGTAATAGCTGaa                                                                                >  2:1720178/1‑150 (MQ=255)
                                                                     gCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTAAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAAc                                                                              <  8:748253/151‑1 (MQ=255)
                                                                      cAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGCTCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGACAcc                                                                             >  3:1808803/1‑151 (MQ=255)
                                                                       aacctGCATAGCGCAGACCAACTGGACCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTGTGGTACGCGGCC‑TCAAACCCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCa                                                                            <  4:1683164/147‑1 (MQ=255)
                                                                       aaaGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAAcc                                                                             >  1:1432567/1‑150 (MQ=255)
                                                                        aaGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCa                                                                            >  3:1401010/1‑150 (MQ=255)
                                                                         aGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAg                                                                          <  7:1585939/151‑1 (MQ=255)
                                                                          gTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGa                                                                         <  7:1887771/151‑1 (MQ=255)
                                                                          gTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGa                                                                         <  5:489473/151‑1 (MQ=255)
                                                                            gCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCTGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCTTCGCCGGTGCCGCCTTGGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGAt                                                                        <  1:1754046/150‑1 (MQ=255)
                                                                                aGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATc                                                                    <  6:259869/150‑1 (MQ=255)
                                                                                 ccgcAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATCCGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGc                                                                  <  6:499368/150‑1 (MQ=255)
                                                                                   gcAGACCTACTGGCTCGATCTGCCCGCCCCAGTCAGATGCTTTAACATACGGGTGTGGTCCGCTGCCTTCAAAACCAGAGATCGCATCGCCGGTGCGGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCa                                                                 <  2:428794/151‑1 (MQ=255)
                                                                                   gcAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGGCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAg                                                                <  3:1618007/151‑1 (MQ=255)
                                                                                    cAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAgg                                                               >  8:1030954/1‑151 (MQ=255)
                                                                                     aGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGt                                                              >  3:345373/1‑151 (MQ=255)
                                                                                         cTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAACACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCt                                                          >  8:1212877/1‑151 (MQ=255)
                                                                                          tACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCtt                                                         >  3:220059/1‑151 (MQ=255)
                                                                                           aCTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGCGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCCGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGAGCCTTc                                                        >  4:1536668/1‑151 (MQ=255)
                                                                                           aCTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAAAATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTc                                                        >  1:1010209/1‑151 (MQ=255)
                                                                                              ggATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATATGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACg                                                     >  5:1664395/1‑151 (MQ=255)
                                                                                              ggATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCAc                                                      <  4:461877/150‑1 (MQ=255)
                                                                                              ggATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACg                                                     >  4:1116631/1‑151 (MQ=255)
                                                                                               gATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACgc                                                    >  4:715227/1‑151 (MQ=255)
                                                                                               gATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACgc                                                    >  1:1958809/1‑151 (MQ=255)
                                                                                                aTCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACgc                                                    >  7:1470702/1‑150 (MQ=255)
                                                                                                 tCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACgca                                                   <  3:388432/150‑1 (MQ=255)
                                                                                                 tCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGCTGCCTTCACgc                                                    >  2:1515009/1‑149 (MQ=255)
                                                                                                  cAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACgcagc                                                 >  4:948653/1‑151 (MQ=255)
                                                                                                    aTCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGcac                                               >  5:776812/1‑151 (MQ=255)
                                                                                                        gCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATcc                                           >  1:598951/1‑151 (MQ=255)
                                                                                                        gCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATc                                            <  8:429112/150‑1 (MQ=255)
                                                                                                         ccAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCa                                          <  8:1343769/151‑1 (MQ=255)
                                                                                                         ccAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCa                                          <  2:1935292/151‑1 (MQ=255)
                                                                                                           aGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATcc                                           >  1:405028/1‑148 (MQ=255)
                                                                                                             cccctctgagttgatttAACATACGCGTTTGTTACGCTGCC‑TCAAAACCAGAGATCGCAGCGCGGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAAGCAAGATAATCGGAGGTGGCTTCACGCAGGTCATCCAGATc                                      <  8:1506939/137‑1 (MQ=255)
                                                                                                             ccccAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTGCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATc                                      <  7:490707/151‑1 (MQ=255)
                                                                                                               ccAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGc                                    >  7:1020694/1‑151 (MQ=255)
                                                                                                                 aGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCg                                  >  6:1148974/1‑151 (MQ=255)
                                                                                                                         gCTTTAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCAt                          <  5:697919/151‑1 (MQ=255)
                                                                                                                            ttATCATACTGTTTTGGTACGCTGCC‑TCAAACCGAGAGATCTCATCGCCGGTGCCGCCTTCGGCCTTCAGTGCATTGGTCATGTAATAGCTGAAACCAAGATAATGGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCAttt                        <  5:381629/150‑1 (MQ=255)
                                                                                                                            ttAACATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCAttt                        <  7:253707/150‑1 (MQ=255)
                                                                                                                              aaCATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTg                      <  8:296976/150‑1 (MQ=255)
                                                                                                                              aaCATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGa                     <  3:913432/151‑1 (MQ=255)
                                                                                                                               aCATACGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGAt                    >  3:1522777/1‑151 (MQ=255)
                                                                                                                                   aCGGGTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGtt                 <  1:483857/150‑1 (MQ=255)
                                                                                                                                     gggTTTGGTACGCTGCCTTCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTa                >  5:1353704/1‑150 (MQ=255)
                                                                                                                                      ggTTTGGTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAACATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAAt             <  8:838914/151‑1 (MQ=255)
                                                                                                                                            gTACGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGa       <  1:1143974/151‑1 (MQ=255)
                                                                                                                                              aCGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGACg     >  7:731671/1‑151 (MQ=255)
                                                                                                                                              aCGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGACg     <  5:1052428/151‑1 (MQ=255)
                                                                                                                                              aCGCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGACg     >  1:135553/1‑151 (MQ=255)
                                                                                                                                                gCTGCC‑TCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGACGCt   >  5:1964265/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CACTTTGTCGTAAGCGCCAAAACCGTTTTCGACAATAAACAGCGGCCTCTGATAACGCTCATACAGTTCGCAAAGTGCATAGCGCAGACCTACTGGATCAATCTGCCAGCCCCAGTCAGATGCTTTAACATACGGGTTTGGTACGCTGCCTTCAAAACCAGAGATCGCATCGCCGGTGCCGCCTTCGGCCTTCACTGCATTGGTCATGTAATAGCTGAAACCAAGATAATCGCAGGTGCCTTCACGCAGCACATCCAGATCGCCGTCTTCCATTTTGATGTTAAATCCGCGACGCTC  >  DH1/838944‑839240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑