Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,701,765 Δ1 bp coding (2129/2244 nt) fhuA ← ferrichrome‑iron receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,701,7650G.98.9% 2118.4 / 17.6 466coding (2129/2244 nt)fhuAferrichrome‑iron receptor
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base . (232/226);  total (237/229)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 467 total aligned reads displayed.

ATGGGCACGGAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCGCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGCGTCAGACC  >  DH1/3701617‑3701910
                                                                                                                                                    |                                                                                                                                                 
  gggCACGGAAATCCGTGCCCCAAAAGAGAAATTAGAATCGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGCTAACATGCAGCCCACg                                                                                                                                              <  2:189709/151‑1 (MQ=255)
     cACGGAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTg                                                                                                                                           >  8:1706774/1‑151 (MQ=255)
      aCGGAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGGCGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTgg                                                                                                                                          >  1:267443/1‑151 (MQ=255)
        ggAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGACATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGag                                                                                                                                        <  6:1021458/151‑1 (MQ=255)
                 tGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATg                                                                                                                               >  7:710882/1‑151 (MQ=255)
                  gCCCCAAAAGAGAAATTCCAAACGGAAGGTTGCGGTTGCAACGACCTAACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGc                                                                                                                              <  8:1635822/151‑1 (MQ=255)
                    cccAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCg                                                                                                                            <  5:1277116/151‑1 (MQ=255)
                    cccAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCg                                                                                                                            >  3:113415/1‑151 (MQ=255)
                     ccAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGAGCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCg                                                                                                                            >  3:1485128/1‑150 (MQ=255)
                       aaaaGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACt                                                                                                                         >  1:1917886/1‑151 (MQ=255)
                       aaaaGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGGTAAAGCAGCTGGCGACGTATTCACGCTCGAACAGGTTGTTAACATGCACACCACGTTGGAGCCAGCCATGCCGAcg                                                                                                                         >  2:769933/1‑150 (MQ=255)
                         aaGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTcg                                                                                                                       >  2:1049731/1‑151 (MQ=255)
                           gagaAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTcgcc                                                                                                                     >  1:1016190/1‑150 (MQ=255)
                              aaaTTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGcc                                                                                                                   >  5:1635489/1‑150 (MQ=255)
                               aaTTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGcc                                                                                                                   >  3:1344018/1‑149 (MQ=255)
                                aTTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGcc                                                                                                                   >  8:211332/1‑148 (MQ=255)
                                aTTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGa                                                                                                                <  8:441078/151‑1 (MQ=255)
                                     aaaCGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCAt                                                                                                            >  3:1937397/1‑150 (MQ=255)
                                     aaaCGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATa                                                                                                           <  1:800779/151‑1 (MQ=255)
                                      aaCGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATaa                                                                                                          <  2:395019/151‑1 (MQ=255)
                                      aaCGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATaa                                                                                                          <  7:1643255/151‑1 (MQ=255)
                                       aCGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAAc                                                                                                         >  3:102582/1‑151 (MQ=255)
                                         ggAAGGTTGCGGTTGCAACGCCCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGt                                                                                                       >  4:71975/1‑151 (MQ=255)
                                         ggAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGt                                                                                                       <  2:919184/151‑1 (MQ=255)
                                         ggAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGt                                                                                                       <  4:347089/151‑1 (MQ=255)
                                          gAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCGCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGt                                                                                                       >  1:1564935/1‑151 (MQ=255)
                                          gAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTa                                                                                                      <  2:702033/151‑1 (MQ=255)
                                             ggttgcggttgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTa                                                                                                   <  1:609633/151‑1 (MQ=255)
                                             ggttgcggttgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTa                                                                                                   <  7:25832/151‑1 (MQ=255)
                                                tgcggttgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAAAAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAAc                                                                                                 <  8:830538/150‑1 (MQ=255)
                                                  cggttgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTTCTAACGCa                                                                                              <  4:275076/151‑1 (MQ=255)
                                                   ggttgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAGGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCAt                                                                                             >  4:856004/1‑151 (MQ=255)
                                                    gttgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATc                                                                                            >  6:1895949/1‑151 (MQ=255)
                                                      tgcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATcc                                                                                           <  3:294707/150‑1 (MQ=255)
                                                       gcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCAc                                                                                         >  3:1767697/1‑151 (MQ=255)
                                                       gcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCAc                                                                                         <  1:1705688/151‑1 (MQ=255)
                                                       gcAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCAc                                                                                         >  6:600189/1‑151 (MQ=255)
                                                           cGACCTGACGTTCTGCGCCCCACAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGAcc                                                                                     <  3:575735/151‑1 (MQ=255)
                                                            gACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCg                                                                                    >  1:1369816/1‑151 (MQ=255)
                                                             aCCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGATAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGt                                                                                   >  6:287782/1‑151 (MQ=255)
                                                              ccTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGCTCATAACGTACTAACGCATCCACGACCGt                                                                                   >  4:952296/1‑150 (MQ=255)
                                                              ccTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGt                                                                                   >  8:100258/1‑150 (MQ=255)
                                                               cTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACGGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGtat                                                                                 >  3:1683309/1‑151 (MQ=255)
                                                                tGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTCAACATGCAGCCCACGTTGCAGCCACCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCCtaa                                                                                 >  6:957887/1‑149 (MQ=255)
                                                                 tactttCTGCGCCCCATAAGCTGCCATAAGTGTTAAAGCAGCTGGCGAGGTATTCACTATCGAACAGGTTGTTAACATGCAGCCCACGTGGGAGCCAGCCATGCCGACTCGGGCCAGATCATAACGTACTAACGCATCCACGACCGtata                                                                                <  8:518442/146‑1 (MQ=255)
                                                                 gACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGtata                                                                                >  8:1101814/1‑150 (MQ=255)
                                                                  aCGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCTACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAAc                                                                              <  4:104750/151‑1 (MQ=255)
                                                                    gTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACtt                                                                            <  3:1211884/151‑1 (MQ=255)
                                                                    gTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACtt                                                                            <  8:139868/151‑1 (MQ=255)
                                                                    gTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACtt                                                                            <  1:357327/151‑1 (MQ=255)
                                                                       gtGCGCCCCAGAAGCAGCCATAAGTGTAAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCAGGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTccc                                                                         <  7:1762533/150‑1 (MQ=255)
                                                                       cTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTccc                                                                         >  5:803864/1‑151 (MQ=255)
                                                                         gcgcCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCACATCATAACGTACTAACGCATCCACGACCGTATAACTTcc                                                                          <  5:1872840/148‑1 (MQ=255)
                                                                               cAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTaaa                                                                 <  4:1334373/151‑1 (MQ=255)
                                                                                aGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTaa                                                                  >  4:823821/1‑149 (MQ=255)
                                                                                  acgcagcCATCCGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGa                                                              <  6:673371/149‑1 (MQ=255)
                                                                                     cagcCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGtt                                                           >  5:491860/1‑151 (MQ=255)
                                                                                     cagcCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGtt                                                           >  2:1432812/1‑151 (MQ=255)
                                                                                      agcCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTa                                                          <  8:1480148/151‑1 (MQ=255)
                                                                                       gcCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAg                                                         <  8:602496/151‑1 (MQ=255)
                                                                                         cATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGcc                                                       <  5:1448856/151‑1 (MQ=255)
                                                                                         cATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGcc                                                       <  5:62126/151‑1 (MQ=255)
                                                                                         cATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGcc                                                       >  4:464798/1‑151 (MQ=255)
                                                                                         cATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGcc                                                       >  4:1789732/1‑151 (MQ=255)
                                                                                          aTAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCg                                                      <  3:1300095/151‑1 (MQ=255)
                                                                                            aaGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGa                                                    <  5:1809586/151‑1 (MQ=255)
                                                                                            aaGTGTTAAAGCAGCGGGCGACGTATGCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGa                                                    <  4:1697752/151‑1 (MQ=255)
                                                                                                gtTAAAGCAGCTGGGCACGTATTCACGAGCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCAc                                                <  6:1714749/151‑1 (MQ=255)
                                                                                                gtTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGCTCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCAc                                                >  6:1390674/1‑151 (MQ=255)
                                                                                                gtTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATccc                                                >  5:1192408/1‑149 (MQ=255)
                                                                                                gtTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCAc                                                <  3:360381/151‑1 (MQ=255)
                                                                                                gtTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCAACGTTGGAGCCAGCCAAGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCa                                                 <  7:1552453/150‑1 (MQ=255)
                                                                                                 ttAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCCGCCCCCGTGGGCGCCAGCCATGCCGACTCGCGCCAGATCATACCGTACTACCGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCAc                                                <  7:833685/150‑1 (MQ=255)
                                                                                                  tAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCAcc                                               >  3:63234/1‑150 (MQ=255)
                                                                                                  tAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCAGACTCGCGCCAGATCAAAACGTACTAACGCATCCACGACCCTATAACTTCCCACTTTAAAGTAGTTAGACGGATCAcc                                               >  4:543255/1‑150 (MQ=255)
                                                                                                     agcagcTGGCCACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGCAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCCCGACCGTATAACTTCCCACATTAAAGGAGTTAGCCGGCTCACCATac                                           >  2:1703489/1‑150 (MQ=255)
                                                                                                       cagcTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACt                                         >  2:567656/1‑151 (MQ=255)
                                                                                                        agcTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATNCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTg                                        >  2:281198/1‑151 (MQ=255)
                                                                                                          cTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTCGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTgg                                       >  2:1940987/1‑150 (MQ=255)
                                                                                                           tGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTgg                                       >  5:320176/1‑149 (MQ=255)
                                                                                                              cGACGTATTCCCGATCGAACCGGTTGTAAACATGCCGCCCACGTGGGCGCCAGCCATGCCCACTCGCCCCAGATCATAACGTACTACCCCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCa                                  <  8:1762451/151‑1 (MQ=255)
                                                                                                              cGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGcc                                   >  7:1217619/1‑150 (MQ=255)
                                                                                                               gACTTATTCACGTTCTAACTGGTTGTTAACATGCAGCCCTCGTTGGAGCCAGCAATGCCGACTCGCGCCAGATCTTAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAg                                 <  2:1307513/151‑1 (MQ=255)
                                                                                                               gACGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAg                                 <  6:328496/151‑1 (MQ=255)
                                                                                                                aCGTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGt                                >  7:57796/1‑151 (MQ=255)
                                                                                                                  gTATTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGtat                              >  8:1236784/1‑151 (MQ=255)
                                                                                                                    aTTCAGGATCGAACAGGTTGGTACCATGCCGCCCACGTTGGCGCCAGCCATGCCGACGCGCGCCAGAGCATAACGTACTAACGCATCCACGACCGGATAACTTCCCACTTTAAAGGAGTTAGCCGGAGCACCATAACTGGAGCCAGTATaa                            <  6:1513341/151‑1 (MQ=255)
                                                                                                                    aTTCACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATaa                            <  1:1111829/151‑1 (MQ=255)
                                                                                                                       cACGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACg                          >  1:727845/1‑150 (MQ=255)
                                                                                                                         cGATCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGCGCCAGTATAACGacc                       >  6:855634/1‑151 (MQ=255)
                                                                                                                          gATCGAACAGGTTGTTAACATGCAGCCCAGGTTGGAGCCAGCCATGCCGACTCGCGCGAGATCATAACGTACTAACGCATCCACGTCCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGacca                      <  2:1195236/151‑1 (MQ=255)
                                                                                                                           aTCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGaccac                     <  4:200849/151‑1 (MQ=255)
                                                                                                                            tCGAACAGGTTGTTAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGaccacc                    >  7:1467563/1‑151 (MQ=255)
                                                                                                                                    gttgttAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCa            <  6:128257/151‑1 (MQ=255)
                                                                                                                                      tgttAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGc          >  5:1107657/1‑151 (MQ=255)
                                                                                                                                       tttACCATGCACCCCACGTTGGCCCCAGCCATGCCGACTCGCTCCAGATCATAACCTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTATCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGc          <  6:527100/149‑1 (MQ=255)
                                                                                                                                       gttAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGc          <  4:1457258/150‑1 (MQ=255)
                                                                                                                                       gttAACATGCAGCCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGCg         <  7:420972/151‑1 (MQ=255)
                                                                                                                                         tAACATGCAGCGCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGCGt        >  3:1578221/1‑151 (MQ=255)
                                                                                                                                          aaCATGCAGCCCACGTTGGAGCCACCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGCGTCa      <  3:846555/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATGGGCACGGAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGCAACGACCTGACGTTCTGCGCCCCAGAAGCAGCCATAAGTGTTAAAGCAGCTGGCGACGTATTCACGATCGAACAGGTTGTTAACATGCAGCGCCACGTTGGAGCCAGCCATGCCGACTCGCGCCAGATCATAACGTACTAACGCATCCACGACCGTATAACTTCCCACTTTAAAGGAGTTAGCCGGATCACCATAACTGGAGCCAGTATAACGACCACCGGTGCCCAGCGTCAGACC  >  DH1/3701617‑3701910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: