Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,668,924 (T)5→6 pseudogene (353/455 nt) lpxA ← hydroxymyristoyl‑ACP dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,668,9191.T97.3% 1854.2 / 32.6 516pseudogene (358/455 nt)lpxAhydroxymyristoyl‑ACP dehydratase
Reads supporting (aligned to +/- strand):  ref base . (8/6);  new base T (240/262);  total (248/268)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 549 total aligned reads displayed.

CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064
                                                                                                                                                        |                                                                                                                                                 
cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGcgcg                                                                                                                                                    <  5:877053/151‑1 (MQ=255)
   tctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttt                                                                                                                                                >  4:447386/1‑151 (MQ=255)
    ctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttt                                                                                                                                               >  8:537260/1‑151 (MQ=255)
      tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttttt                                                                                                                                             >  1:619686/1‑150 (MQ=255)
      tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttt                                                                                                                                               <  2:921943/149‑1 (MQ=255)
      tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttt                                                                                                                                                <  7:1493235/148‑1 (MQ=255)
        cacaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcg                                                                                                                                           >  1:1814134/1‑148 (MQ=255)
        cacaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcg                                                                                                                                           <  2:1821270/151‑4 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         <  7:1370123/151‑6 (MQ=255)
              ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGt                                                                                                                                       <  2:1270149/150‑1 (MQ=255)
              ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGt                                                                                                                                       <  2:1206565/150‑1 (MQ=255)
               gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTg                                                                                                                                      >  5:234477/1‑150 (MQ=255)
                 ggTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACt                                                                                                                                   <  8:1826910/151‑1 (MQ=255)
                 ggTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACt                                                                                                                                   >  4:382387/1‑151 (MQ=255)
                   ttGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTc                                                                                                                                 >  3:148283/1‑151 (MQ=255)
                   ttGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTc                                                                                                                                 >  7:1002710/1‑151 (MQ=255)
                      gATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCAt                                                                                                                              <  3:203844/151‑1 (MQ=255)
                       aTGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATg                                                                                                                             <  6:465418/151‑1 (MQ=255)
                         gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGAt                                                                                                                           <  2:364285/151‑1 (MQ=255)
                            caAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAt                                                                                                                        >  3:1232908/1‑151 (MQ=255)
                             aaaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCTATGATCAtt                                                                                                                       <  7:1084536/151‑1 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      >  6:589578/1‑151 (MQ=255)
                               aGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTg                                                                                                                     >  8:1149862/1‑151 (MQ=255)
                               aGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCACGATCATTTg                                                                                                                     <  1:1673296/151‑1 (MQ=255)
                                ggCGGATTTATCAATCACGTATCAGGCCTCCCTGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTCACTTCCATGATCACTTGa                                                                                                                    <  6:865563/151‑1 (MQ=255)
                                ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTg                                                                                                                     >  2:437494/1‑150 (MQ=255)
                                ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGa                                                                                                                    <  1:1299831/151‑1 (MQ=255)
                                 gCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTAATTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGAt                                                                                                                   >  4:1734199/1‑151 (MQ=255)
                                  gggttttATCAATCACGTATCAGGCGTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAATCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATTATCATTTGATc                                                                                                                  <  5:1583458/147‑1 (MQ=255)
                                    gATTTATCAATCACGTATCAGGACTCCAGTCTACGAGCACACATCATAGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCg                                                                                                                 <  4:1794912/150‑1 (MQ=255)
                                         atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGc                                                                                                           <  1:881046/151‑1 (MQ=255)
                                              tcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCATGATCATTTGATCGCCAGGCACGAcc                                                                                                     >  5:1870850/1‑151 (MQ=255)
                                                 cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg                                                                                                   <  4:1071053/151‑1 (MQ=255)
                                                  gTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCggg                                                                                                  <  6:689381/151‑1 (MQ=255)
                                                    aTCAGGCCTCCCTGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg                                                                                                <  4:1640602/151‑1 (MQ=255)
                                                    aTCAGGCCTCCCGTCTACGAGCACACTTCATGGTTGCTTCGCAAACTACTTTACCATCGAGCAGAGCAACCCCTTTAATACGGGTCAGGCCGCGGCGCGTTTTTTCGAATGTGACTTCCATGATCATTTGATCTCCAGGGACGACCGGgcg                                                                                                <  4:1440848/151‑1 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgc                                                                                                 <  1:271275/150‑1 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGccg                                                                                                >  8:1603531/1‑151 (MQ=255)
                                                      cAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTAGAAAGTGACTTCCATGATCATTTGATCGCCCGGCACCACCGGGCGCt                                                                                              >  8:488813/1‑151 (MQ=255)
                                                         gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa                                                                                           <  6:1726699/151‑1 (MQ=255)
                                                             cccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc                                                                                        <  3:518676/150‑1 (MQ=255)
                                                              ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGCCACGACCGGGCGCTTGAAgcgc                                                                                      >  6:1130934/1‑151 (MQ=255)
                                                               cGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcg                                                                                     <  1:1027606/151‑1 (MQ=255)
                                                                ggCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcgc                                                                                    <  8:1976782/151‑1 (MQ=255)
                                                                    aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCGTCg                                                                                >  7:691532/1‑151 (MQ=255)
                                                                    aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc                                                                                 <  6:1095453/150‑1 (MQ=255)
                                                                    aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc                                                                                 <  3:1372447/150‑1 (MQ=255)
                                                                     cGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGt                                                                               <  4:840433/151‑1 (MQ=255)
                                                                       aGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCa                                                                             <  5:1474497/151‑1 (MQ=255)
                                                                          acacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATa                                                                          <  7:1899268/151‑1 (MQ=255)
                                                                             catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTCAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTAATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa                                                                       <  7:548143/151‑1 (MQ=255)
                                                                             catcatCGTTCCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa                                                                       <  8:1272634/151‑1 (MQ=255)
                                                                              atcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAg                                                                      <  8:801239/151‑1 (MQ=255)
                                                                                catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg                                                                    >  8:1474083/1‑151 (MQ=255)
                                                                                  tcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa                                                                   >  6:33257/1‑150 (MQ=255)
                                                                                  tcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa                                                                   >  3:884753/1‑150 (MQ=255)
                                                                                   cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGaa                                                                  <  3:1138859/150‑1 (MQ=255)
                                                                                    gTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagt                                                                >  6:1023623/1‑151 (MQ=255)
                                                                                    gTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTAGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTTCAGCGCGCTTCGTCACTACCAGCGAagt                                                                >  5:1094298/1‑151 (MQ=255)
                                                                                     ttGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagt                                                                >  6:1442809/1‑150 (MQ=255)
                                                                                       gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtagt                                                             >  4:651046/1‑151 (MQ=255)
                                                                                          tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc                                                           >  3:1017005/1‑150 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGACGTAGTACa                                                          <  3:1807522/150‑1 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAccg                                                         >  6:1661254/1‑149 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAg                                                         <  3:362394/151‑1 (MQ=255)
                                                                                              aaaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAATGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc                                                      >  1:1515140/1‑151 (MQ=255)
                                                                                               aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc                                                      >  8:1010797/1‑150 (MQ=255)
                                                                                               aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc                                                      >  7:220338/1‑150 (MQ=255)
                                                                                               aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCa                                                     <  4:117310/151‑1 (MQ=255)
                                                                                                  tactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAccc                                                  >  7:46746/1‑151 (MQ=255)
                                                                                                   actTTACCATCGACCAGAGCACCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCAAGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCg                                                 <  2:159347/151‑1 (MQ=255)
                                                                                                      ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg                                                <  5:643419/149‑1 (MQ=255)
                                                                                                       tACCATCGACCAGAGCATCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGtt                                              <  2:1477695/150‑1 (MQ=255)
                                                                                                          cATCGACCACAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAg                                          <  3:1701217/151‑1 (MQ=255)
                                                                                                           aTCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCCTCAATACCAGCGAAGTACTACAGCTCACCCGGTTCCAGt                                         >  1:751430/1‑151 (MQ=255)
                                                                                                              gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt                                      <  7:82309/151‑1 (MQ=255)
                                                                                                              gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt                                      >  8:638123/1‑151 (MQ=255)
                                                                                                               aCCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCCGTTTTc                                     >  5:101321/1‑151 (MQ=255)
                                                                                                                    agCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACg                                <  5:447330/151‑1 (MQ=255)
                                                                                                                      cAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCt                              <  7:1266468/151‑1 (MQ=255)
                                                                                                                       aaCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt                             >  1:1215062/1‑151 (MQ=255)
                                                                                                                       aaCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt                             >  2:84420/1‑151 (MQ=255)
                                                                                                                       aaCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGAGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt                             >  2:1370193/1‑151 (MQ=255)
                                                                                                                         ccccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt                           >  1:1891910/1‑151 (MQ=255)
                                                                                                                           ccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACCCTTTTa                          >  1:1584411/1‑150 (MQ=255)
                                                                                                                              ttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaaa                        <  3:1437040/149‑1 (MQ=255)
                                                                                                                               tAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGc                     >  3:670533/1‑151 (MQ=255)
                                                                                                                                  aaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCATTACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTGCTACGCTTTTAAACGcc                    >  4:1291598/1‑149 (MQ=255)
                                                                                                                                  aaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGccgg                  <  3:452334/151‑3 (MQ=255)
                                                                                                                                   aCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc                    >  1:464088/1‑148 (MQ=255)
                                                                                                                                      ggTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATa              <  2:1761648/151‑1 (MQ=255)
                                                                                                                                         cAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCt           <  5:1192495/151‑1 (MQ=255)
                                                                                                                                          aGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg          >  2:1922011/1‑151 (MQ=255)
                                                                                                                                          aGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg          >  6:1943730/1‑151 (MQ=255)
                                                                                                                                          aGGACGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACAGGGCGCTTTAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg          >  2:659800/1‑151 (MQ=255)
                                                                                                                                           ggCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGt         >  4:209368/1‑151 (MQ=255)
                                                                                                                                            gCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGtt        <  3:998397/151‑1 (MQ=255)
                                                                                                                                              cgcgGCGCGTTTTTTCGAAAGTGACTTGCATGATCATTTGATCGCCAGGCACGACCGGGCGCGTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCCGTTCCAGTTTTCCTACGCGTTTAAACGCCAGAATACCTGTTGc      >  5:1366789/1‑151 (MQ=255)
                                                                                                                                              cgcgGCGCGTTTTTTCGAAAGTGACGTCCATGGTCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCGTCGTCAATACCAGCGAAGTAGTTCAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGc      >  8:30438/1‑151 (MQ=255)
                                                                                                                                                cggcgCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCTCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCt    <  4:746774/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                 
CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: