Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | DH1 | 3,668,924 | (T)5→6 | pseudogene (353/455 nt) | lpxA ← | hydroxymyristoyl‑ACP dehydratase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 3,668,919 | 1 | . | T | 97.3% | 1854.2 / 32.6 | 516 | pseudogene (358/455 nt) | lpxA | hydroxymyristoyl‑ACP dehydratase |
Reads supporting (aligned to +/- strand): ref base . (8/6); new base T (240/262); total (248/268) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 549 total aligned reads displayed.
CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT > DH1/3668769‑3669064 | cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGcgc‑g < 5:877053/151‑1 (MQ=255) tctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑ttt > 4:447386/1‑151 (MQ=255) ctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑tttt > 8:537260/1‑151 (MQ=255) tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑tttttt > 1:619686/1‑150 (MQ=255) tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑tttt < 2:921943/149‑1 (MQ=255) tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑ttt < 7:1493235/148‑1 (MQ=255) cacaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑ttttttcg > 1:1814134/1‑148 (MQ=255) cacaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑ttttttcg < 2:1821270/151‑4 (MQ=255) caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑ttttttcgaa < 7:1370123/151‑6 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGt < 2:1270149/150‑1 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGt < 2:1206565/150‑1 (MQ=255) gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTg > 5:234477/1‑150 (MQ=255) ggTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACt < 8:1826910/151‑1 (MQ=255) ggTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACt > 4:382387/1‑151 (MQ=255) ttGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTc > 3:148283/1‑151 (MQ=255) ttGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTc > 7:1002710/1‑151 (MQ=255) gATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCAt < 3:203844/151‑1 (MQ=255) aTGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATg < 6:465418/151‑1 (MQ=255) gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGAt < 2:364285/151‑1 (MQ=255) caAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCAt > 3:1232908/1‑151 (MQ=255) aaaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCTATGATCAtt < 7:1084536/151‑1 (MQ=255) aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCAttt > 6:589578/1‑151 (MQ=255) aGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTg > 8:1149862/1‑151 (MQ=255) aGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCACGATCATTTg < 1:1673296/151‑1 (MQ=255) ggCGGATTTATCAATCACGTATCAGGCCTCCCTGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTCACTTCCATGATCACTTGa < 6:865563/151‑1 (MQ=255) ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTg > 2:437494/1‑150 (MQ=255) ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGa < 1:1299831/151‑1 (MQ=255) gCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTAATTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGAt > 4:1734199/1‑151 (MQ=255) gggttttATCAATCACGTATCAGGCGTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAATCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATTATCATTTGATc < 5:1583458/147‑1 (MQ=255) gATTTATCAATCACGTATCAGGACTCCAGTCTACGAGCACACATCATAGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCg < 4:1794912/150‑1 (MQ=255) atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGc < 1:881046/151‑1 (MQ=255) tcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTT‑CATGATCATTTGATCGCCAGGCACGAcc > 5:1870850/1‑151 (MQ=255) cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg < 4:1071053/151‑1 (MQ=255) gTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCggg < 6:689381/151‑1 (MQ=255) aTCAGGCCTCCCTGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg < 4:1640602/151‑1 (MQ=255) aTCAGGCCTCCCGTCTACGAGCACACTTCATGGTTGCTTCGCAAACTACTTTACCATCGAGCAGAGCAACCCCTTTAATACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAATGTGACTTCCATGATCATTTGATCTCCAGGGACGACCGGgcg < 4:1440848/151‑1 (MQ=255) aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgc < 1:271275/150‑1 (MQ=255) aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGccg > 8:1603531/1‑151 (MQ=255) cAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTAGAAAGTGACTTCCATGATCATTTGATCGCCCGGCACCACCGGGCGCt > 8:488813/1‑151 (MQ=255) gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa < 6:1726699/151‑1 (MQ=255) cccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc < 3:518676/150‑1 (MQ=255) ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGCCACGACCGGGCGCTTGAAgcgc > 6:1130934/1‑151 (MQ=255) cGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcg < 1:1027606/151‑1 (MQ=255) ggCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcgc < 8:1976782/151‑1 (MQ=255) aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGG‑TTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCGTCg > 7:691532/1‑151 (MQ=255) aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc < 6:1095453/150‑1 (MQ=255) aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc < 3:1372447/150‑1 (MQ=255) cGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGt < 4:840433/151‑1 (MQ=255) aGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCa < 5:1474497/151‑1 (MQ=255) acacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATa < 7:1899268/151‑1 (MQ=255) catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTCAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTAATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa < 7:548143/151‑1 (MQ=255) catcatCGTTCCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa < 8:1272634/151‑1 (MQ=255) atcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAg < 8:801239/151‑1 (MQ=255) catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg > 8:1474083/1‑151 (MQ=255) tcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa > 6:33257/1‑150 (MQ=255) tcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa > 3:884753/1‑150 (MQ=255) cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGaa < 3:1138859/150‑1 (MQ=255) gTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagt > 6:1023623/1‑151 (MQ=255) gTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTAGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTTCAGCGCGCTTCGTCACTACCAGCGAagt > 5:1094298/1‑151 (MQ=255) ttGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagt > 6:1442809/1‑150 (MQ=255) gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtagt > 4:651046/1‑151 (MQ=255) tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc > 3:1017005/1‑150 (MQ=255) cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGACGTAGTACa < 3:1807522/150‑1 (MQ=255) cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAccg > 6:1661254/1‑149 (MQ=255) cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAg < 3:362394/151‑1 (MQ=255) aaaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAATGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc > 1:1515140/1‑151 (MQ=255) aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc > 8:1010797/1‑150 (MQ=255) aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc > 7:220338/1‑150 (MQ=255) aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCa < 4:117310/151‑1 (MQ=255) tactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAccc > 7:46746/1‑151 (MQ=255) actTTACCATCGACCAGAGCACCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCAAGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCg < 2:159347/151‑1 (MQ=255) ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg < 5:643419/149‑1 (MQ=255) tACCATCGACCAGAGCATCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGtt < 2:1477695/150‑1 (MQ=255) cATCGACCACAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAg < 3:1701217/151‑1 (MQ=255) aTCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCCTCAATACCAGCGAAGTACTACAGCTCACCCGGTTCCAGt > 1:751430/1‑151 (MQ=255) gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt < 7:82309/151‑1 (MQ=255) gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt > 8:638123/1‑151 (MQ=255) aCCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCCGTTTTc > 5:101321/1‑151 (MQ=255) agCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACg < 5:447330/151‑1 (MQ=255) cAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCt < 7:1266468/151‑1 (MQ=255) aaCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt > 1:1215062/1‑151 (MQ=255) aaCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt > 2:84420/1‑151 (MQ=255) aaCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGAGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt > 2:1370193/1‑151 (MQ=255) ccccTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt > 1:1891910/1‑151 (MQ=255) ccTTTAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACCCTTTTa > 1:1584411/1‑150 (MQ=255) ttAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaaa < 3:1437040/149‑1 (MQ=255) tAAAACGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGc > 3:670533/1‑151 (MQ=255) aaCGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCATTACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTGCTACGCTTTTAAACGcc > 4:1291598/1‑149 (MQ=255) aaCGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGccgg < 3:452334/151‑3 (MQ=255) aCGGGTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc > 1:464088/1‑148 (MQ=255) ggTCAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATa < 2:1761648/151‑1 (MQ=255) cAGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCt < 5:1192495/151‑1 (MQ=255) aGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg > 2:1922011/1‑151 (MQ=255) aGGCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg > 6:1943730/1‑151 (MQ=255) aGGACGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACAGGGCGCTTTAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg > 2:659800/1‑151 (MQ=255) ggCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGt > 4:209368/1‑151 (MQ=255) gCCGCGGCGC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGtt < 3:998397/151‑1 (MQ=255) cgcgGCGC‑GTTTTTTCGAAAGTGACTTGCATGATCATTTGATCGCCAGGCACGACCGGGCGCGTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCCGTTCCAGTTTTCCTACGCGTTTAAACGCCAGAATACCTGTTGc > 5:1366789/1‑151 (MQ=255) cgcgGCGC‑GTTTTTTCGAAAGTGACGTCCATGGTCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCGTCGTCAATACCAGCGAAGTAGTTCAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGc > 8:30438/1‑151 (MQ=255) cggcgC‑GTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCTCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCt < 4:746774/151‑1 (MQ=255) | CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT > DH1/3668769‑3669064 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |