Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8370CG29.2% 162.6 / 26.0 107intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (52/16);  new base G (28/0);  total (89/18)
Fisher's exact test for biased strand distribution p-value = 4.77e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906687‑3906987
                                                                                                                                                      |                                                                                                                                                      
gcccgccccacgacgcccccTCGCTCCTACAAAGTCTTTTATATTCCTCCGTCTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCAAACGAGGCg                                                                                                                                                       <  3:1513786/136‑1 (MQ=2)
   gcgtCAACTCCCCCCCCTCGCACCAAAAACCACTTTTATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGat                                                                                                                                                     <  3:1284332/148‑1 (MQ=255)
     cccaacgacccccccTCGGAGCAAAAACCACTTTTAAATTAATGTCAGTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGata                                                                                                                                                    <  5:1895705/142‑1 (MQ=17)
                ccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGGACCACACGCGGCTCTTTCCAAAAAAggt                                                                                                                                        >  1:919635/1‑149 (MQ=18)
                     cGCACCAAAAACCACTTTGAAATTTCTCGGACTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGa                                                                                                                                     <  4:1611567/149‑1 (MQ=255)
                     cGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACCCACCAAAGAACTGTACATCAAAAAAAGTAACATg                                                                                                                                   >  1:1648314/1‑151 (MQ=18)
                      gctccAATAACCTCGTTTATATTTCTCTCTCTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGa                                                                                                                                  <  8:741819/148‑1 (MQ=18)
                      gCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATACGATAATTCCAAAAAAGTTAGATgc                                                                                                                                  >  7:139929/1‑150 (MQ=18)
                       cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGTAAATCAAATAATGTTAGATGa                                                                                                                                  >  5:1580704/1‑150 (MQ=18)
                       cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAATCGTTAAATCAAAAAAAGtttgatcc                                                                                                                                  >  8:807140/1‑143 (MQ=17)
                       cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTAAAGTACCCCCCCAAGCACCCAAAGAGGGGATATAAAAAAAAGTAAAATGa                                                                                                                                  >  8:1755576/1‑150 (MQ=21)
                        aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAAAATGGGGAATTCAAATAAAGTTTGATGACt                                                                                                                                >  1:1946681/1‑151 (MQ=18)
                        aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACTATAAATCAAAAAAAGTTAGAtcaat                                                                                                                                >  6:909165/1‑147 (MQ=17)
                         ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATAAAGGAGAAATCCAATAAAGTAAGAtcaatg                                                                                                                               >  6:247576/1‑146 (MQ=18)
                          cAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGATAAATCAATAAAAGtttgatcaatgt                                                                                                                              >  6:1713852/1‑140 (MQ=17)
                            aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTAAAATACCCCCCCACGCACCCAAACAGACGaaaaaaaaaaaaaaaaaaaatataaa                                                                                                                             >  8:449480/1‑136 (MQ=17)
                              aaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGATTTTATATCCTAAAAAGTTAGTTGCAtgtggtt                                                                                                                          >  3:861421/1‑148 (MQ=17)
                                 cACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCCAAAAAAGTTTGTTGCCtgtggtaagt                                                                                                                       >  1:338163/1‑145 (MQ=18)
                                   cGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCAGCAACCCAGGGGAAAACAAAAAAAGGAAGTTgccggtccgtatgtg                                                                                                                     >  7:974853/1‑137 (MQ=17)
                                    gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGGACCAATCATGGGGAGTTCAATTAAAGTTAGATgcttgttgtttggtgg                                                                                                                    >  3:1232199/1‑136 (MQ=14)
                                    gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGGAGATCAAATAAAGTTTCTTGACTGTGGGAAGGTgg                                                                                                                    >  5:561400/1‑151 (MQ=18)
                                    gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATACGGGAATTCAAATAAAGTTAGATGACtgatatatggtgg                                                                                                                    >  7:1002361/1‑140 (MQ=17)
                                      ttttatacctgcgtcTGGGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTATTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGc                                                                                                                   <  5:1042130/136‑1 (MQ=21)
                                      ttatatTGCTGACTGTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCACCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGc                                                                                                                   <  7:1107922/148‑1 (MQ=18)
                                      ttaaataggtgcgagtGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGc                                                                                                                   <  3:1804701/135‑1 (MQ=255)
                                          atatttggctcttggCGAATGTGGCGGAATTGGTAGACGCTCTAGCTTCATGTGTTATTGTTCTTACGGACGTTTGGGTTCAAGCCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAAt                                                                                                              <  1:807216/138‑1 (MQ=255)
                                          atTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGCTAAATCCATTAAAGTTACATGACTGTTGGAAGCTGCCGGAAt                                                                                                              >  7:799998/1‑151 (MQ=14)
                                           ttGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTAAAGTCCCCCCCCTCGCCCCAAAAAAACATAAAACAATTAAAGTATGATCCCTGATCTAAGGTGGCGGAAtt                                                                                                             >  8:538370/1‑151 (MQ=14)
                                            agctccacgtttgCGAAGGGGGCGGAATTGGTAGACGCGCTAGCGTCAGGTGTTAGTGTTATTACGGACGTGGCGGTGCAAGGCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTg                                                                                                            <  7:634236/139‑1 (MQ=21)
                                                 gCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGATCAAAAATAGTAAGATGCCTGTGCGAAGGTGGCGGATTTGGTAgc                                                                                                       >  3:1845121/1‑150 (MQ=18)
                                                  tgatttgCGAAGGGGGCGGAAGGGTTATACGATCTAGCTGCATGTGTTATATTAACTAATGACGTCCCGGTGAAAGTCCCACCCCTCGGACCAACCGCTCCGAAATCAAAAAAAGTAATCTTACTTTGCGAAGGTGGCGGAATTGGTa                                                                                                         <  1:873761/142‑1 (MQ=255)
                                                  caccgcgctacgggggCGGAATGGGTAGACGCCCTAGCTTCAGGTGTTAGTGTTCTTTCGTACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGCTATCTAAAAAATTACGATGACTGTGCGAAGGTGGCGGAATTGGTAGAc                                                                                                      <  7:1318015/137‑1 (MQ=21)
                                                   ggtatgCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTaa                                                                                                                                       <  2:718346/112‑1 (MQ=255)
                                                                      aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGAGATATCAAAAAAAGTATGATGAGTGTGGGAAGGTGGCGGAATTGGTATAAGTGCTATCATCAggatttat                                                                                  >  3:1316047/1‑145 (MQ=18)
                                                                       ttGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAACGAGGGGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGGGCTAGCTTCAGGTGTTAgt                                                                                 >  7:416097/1‑151 (MQ=255)
                                                                         ggTAGACGCGCTAGCTTCAGGTGTTAGTGTTATTAGGTTAGGGGGGGTTCATGTCCCACCACTATCAATAAAAGATTATATATCAAATAATGTATTATGAGTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAgtg                                                                                <  8:111962/150‑1 (MQ=12)
                                                                             gACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGTCATTCCAAAAAAGTTTGGTGAGTGTGGTTAGGTGGAGGAATTTATATAAGCTATATCTTCATGAGTTAATGTcat                                                                            >  5:331150/1‑148 (MQ=2)
                                                                              aCGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTTGATGACTGTTGTAAGGTGGCGGAATTGGTATAAGTGCTAGCTTCAGGTGTTActgtaaat                                                                           >  3:1575557/1‑146 (MQ=21)
                                                                                   cTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGTACGGGAAATCAAAAAAAGTAAGATGACTGTGGTAAGGTCGCGGAATTCGAATAAGGGCTATCATCAGGTGTCActgtaaaaaagccc                                                                     >  1:1687044/1‑141 (MQ=12)
                                                                                   cTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGAAATCAAAAAAAGTTTCTTGACTGTGTTAACGTGGCGGAATTGGTTGAAGCGGTATCATCATGTTTAAATGTCCTTAAGGAc                                                                     >  8:1251613/1‑151 (MQ=12)
                                                                                        ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATTTCAATTAAAGTTTGATGCCTGTTGTTTGGTGGCGGATTTTTTTTACTGGTTAGCTTCATGTTGTAGTGTAATTAAGGCCGTggg                                                                >  3:1193507/1‑151 (MQ=9)
                                                                                           aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGAGATCAAAAAAAGTTTGATGCCTGTGGGTAGGTGGCGGAATTGGTAGAAGTCAGATCATCAGGTGTTAGTGTAAATAAGGCCGGGGGGGt                                                             >  5:1454841/1‑151 (MQ=16)
                                                                                            ggTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGCGATTTCAAAAAAAGTAAGATGACAGTTGGAAGGTGGCGGAATTGGTAGCAGCGCGAGCTTCAGGTGTTAGTGTCATTACGGACGTGTGGGtg                                                            >  1:1918635/1‑150 (MQ=25)
                                                                                            ggTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTAAGTTGAATGTTGTTAGGTGGCGGAATTGATATAATAGAGAGCTGCATGATTTAGTGTAATATAGGACGTGGGGGtt                                                            >  8:1349352/1‑151 (MQ=12)
                                                                                             gtgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATTTCAAATATAGTTAGATGCCTGTTGGTAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTGAGTGTCCTTAGGGACGTGGGGGtgg                                                           >  4:1193556/1‑149 (MQ=21)
                                                                                             gtgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTTAGATGACTGTTGTAAGGTGCCGGAATTGGTATACGAGATATCTTCAGGTGTTACTGTAAATAAGGACGTCGCGGttg                                                           >  5:988010/1‑150 (MQ=21)
                                                                                               cgcactggccgtccGGACCGGGCGGTGCACGTCCCCCCCCCCGCACTATATTAATCGATATCCAGAAAAGTCAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCTCTAGCTTCAGCTGTTAGTGTCCTTACGGACGTGGGGGTTc                                                           <  8:482975/136‑1 (MQ=12)
                                                                                                ttAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAAAGTTTGATGCGTGTGGTTTGGTGGCGGATTTGGTTGAAGGGCTAGCTTCAGGTGTTAGTGTAATTACGGCCGTGGGGGttgg                                                          >  3:517304/1‑147 (MQ=21)
                                                                                                 tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTTGGAAGGTGGCGGAATTGGTAGACGAGCTAGCATCAGGTGTTACTGTCCTTACGGCCGTGGGGGTTGAAgg                                                       >  1:1335393/1‑150 (MQ=37)
                                                                                                  aGTGTTCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCGCGCACCCAACGAGGGGGTATGAAAAAAAGGTAGGTGACTGGGGGGAGGGGGCGGGATTGGTATGCCCGCTTGCGTCCGGTGTTAGTGTCCTTAGGGCCGTggggggtgcccgcc                                                     >  1:548047/1‑142 (MQ=255)
                                                                                                    tctgctgacgttcGTGTGTGTTCCTGTCCCCCCCCTCGCACCATACCAGTACATATCTTTTATATTTATATGACTGTGCTAAGGTTGCTTAATTTGTATACGCTCTAGCTTCATGTGTTAGTGTCATTAGGGACGTGGGGGTTCAAGTcc                                                     <  8:1098129/138‑1 (MQ=9)
                                                                                                         ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGAGATCAAAAAATGTTAGTTGCCTGTTCTTAGCTGGCGGAATTGGTATCCGGGGGATCTGCAGGTGTTACTGTCGTTACGGACGTGGGGGTTCAAGGcaccccg                                                >  3:746279/1‑149 (MQ=19)
                                                                                                              gACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGCGAGATCATAAAATGTATGATGAGTGTGGGTAGGTGGCGGAATTGGTTTCCGCGCAAGCTGCAGGTGTGAGTCTCATTTCGCCCGTGCGGGTGGAAGTAACCGCCCACGcc                                          >  3:1764766/1‑150 (MQ=255)
                                                                                                              gACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGATTTGGTAGACGAGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTacacccccaagcc                                          >  8:100714/1‑146 (MQ=25)
                                                                                                                cGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCAAAAAAAGTATGATGACTGTTGTATGGTGGCGGAATTGGTATAAGCGCTAGCTTCAGGTCGTAGTGTCCTTACGGACCTGGGGGTTATAGTcgcacccccgagcac                                        >  1:1851242/1‑144 (MQ=19)
                                                                                                                 gTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGcccc                                        >  7:1402825/1‑150 (MQ=255)
                                                                                                                         tCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCATTACGGACGTCGGGGTTGAAGTAACCCCCCACGCCCCaaatttgtt                               >  8:74864/1‑144 (MQ=21)
                                                                                                                           aaGTCCCCCCCCTCGCACCAAACGAGGGGATATCAATAATAGTAAGATGACTGTGCTTAGGTGGCGGAATTGGTAGCCGCGGTAGCTTCAGGTTGTAGTGTCCTTAAGGCCGTGGGGGTGGAAGGACCCCCCCTCGCCCCaaatttgttt                              >  1:424026/1‑142 (MQ=18)
                                                                                                                            aGTCCCCCCCCTCGCACCAAAGGAGGCTATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTGGCCCCAATTTTGtttgc                            >  5:223300/1‑149 (MQ=21)
                                                                                                                            aGTCCCCCCCCTCGCACCAAACGAGGAGAAATCAAAAAAAGTAAGATGCCTGTGGTATGCTCGCGGATTTGGTAGACGCGATAGCTTCAGGTGTGAGTGTAATTACGGACGTCGCGGTTCTAGTAAACCCGAAGGCACCAAATATCATTAc                            >  1:1745940/1‑151 (MQ=16)
                                                                                                                               ccccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCTAAGGTGGCGGAATTGGTATACGGGCGAGCTGCAGGTGTTAGTGTCGTAACGGACGTGGGGGTTCAAGGACCCCCGCAGGCCCCAATTaggttttata                          >  4:24114/1‑141 (MQ=255)
                                                                                                                                 ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAAGTCCCCCCCCGCGCCCCAATTATCTTTACTTccg                       >  7:307744/1‑150 (MQ=255)
                                                                                                                                  cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCACGTCCCCCCCCTCGCGCCAATTATCTTTACTTccgt                      >  6:1817091/1‑149 (MQ=255)
                                                                                                                                    cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTGCCCCCCCACGCCCCAATAAACTTTAATTCCgtt                     >  8:180013/1‑150 (MQ=21)
                                                                                                                                    cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtttg                    >  5:136815/1‑150 (MQ=255)
                                                                                                                                     ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGCAGTCCCCCCCCTCGCCCCAATTATCTTTACTTGCtttat                   >  5:368959/1‑149 (MQ=255)
                                                                                                                                     ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCt                   >  2:605356/1‑151 (MQ=255)
                                                                                                                                      cccGCCCCACACCACGCGACCTCAATAATATTTAGATGACTGTGCGAAGGTGGCGGAATTGGTCGACGCGCTTGCTTGAGGTGTTAGTGTCCTTACTGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCtt                  <  7:115277/149‑1 (MQ=17)
                                                                                                                                       tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTg                 >  2:234545/1‑151 (MQ=255)
                                                                                                                                       tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACGTACGTTCTTg                 >  6:1758201/1‑151 (MQ=255)
                                                                                                                                       tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTAAAGTGCCCCCCCCACCACCAAATATCTTTAAttgcgttattt                 >  8:1049316/1‑142 (MQ=18)
                                                                                                                                        cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCCCCAATTATATTTACTTCCTTTCTTGt                >  3:959282/1‑151 (MQ=255)
                                                                                                                                        cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAACGCCCCCCCCCCGCACCAAATATCATTACATcagatcatct                >  4:1624647/1‑142 (MQ=18)
                                                                                                                                         gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATAGTTACTTCCTTTCTTGtt               >  4:1835591/1‑151 (MQ=255)
                                                                                                                                         gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCCCCAATTATGTTTAATTCATTTCTTGtt               >  2:1638152/1‑151 (MQ=21)
                                                                                                                                          cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAAGTCCCCCCCCGCCCCCCAAATATCTTTAAGTACTTTTTTGttt              >  1:1925902/1‑151 (MQ=18)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtttg             >  1:1403369/1‑150 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtttg             >  3:1120557/1‑150 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttc             >  7:628637/1‑151 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCCCCAATTATATTTACTTCCTTTCTTGtttg             >  8:595658/1‑150 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTCCGAAGGTGGCGGAATTGGTAGTCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTGCCCCCCCGCCCCCCAATTATCATTACTTCCGTTCTTGttt              >  5:1346148/1‑150 (MQ=21)
                                                                                                                                            ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct            >  5:268063/1‑151 (MQ=255)
                                                                                                                                            ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct            >  5:1836770/1‑151 (MQ=255)
                                                                                                                                            ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCCGCCCCCAATAAGTTTAAGTCCTTTGttttttgt            >  1:762160/1‑149 (MQ=18)
                                                                                                                                             cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttctt           >  2:98641/1‑151 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATGGTTACTTCCTTTCGTGTttcttg          >  7:1447263/1‑150 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATCTTTTCTTGCTTTGTtgtttgttg          >  8:1536022/1‑150 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTCGGGGTTAAAGTCCCGCCCCTCCCCCCAATTATATTTACATCCTTTGTTGTttcttg          >  5:1948567/1‑150 (MQ=21)
                                                                                                                                               aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACGTCCTTTCTTGTTTCTTcc         >  4:1865569/1‑151 (MQ=255)
                                                                                                                                                 cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGGGCAGTGCCCCCCCCCGCCCCCATAATATTTACTTGCGTTGTGGTttgttggat       >  7:772399/1‑147 (MQ=18)
                                                                                                                                                    ggCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATATTTACATGCTTTGTTGTTTGTTGCTTGAt    >  8:1398491/1‑151 (MQ=18)
                                                                                                                                                    ggCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATATATTTACTTCCTTTCTTGTTTGTTGCTTGAt    >  4:1458679/1‑151 (MQ=255)
                                                                                                                                                    ggCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACCCACCAATTATGTTTACTTCCTTTCTTGTTTATTCATTGAt    >  2:1228316/1‑151 (MQ=255)
                                                                                                                                                    ggCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTAAAGTCCCCCCCCACGCACCAATTATCTTTACTTGCTTTCTTGTTTCTTCCATGAt    >  4:624331/1‑151 (MQ=255)
                                                                                                                                                     gCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCGTTTCTTGTTTCTTGCTTGAtt   >  1:705892/1‑151 (MQ=255)
                                                                                                                                                     gCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACCCACCAATTATCTTTACATCCTTTCTTGTTTGTTCCTTGAtt   >  3:618363/1‑151 (MQ=255)
                                                                                                                                                      cGATATCAAAAAAAGTAAGATGACTGTGCGAGGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAATTATCTTTACGTCCTTTCTTGTTTCTTCCTTGAttt  >  3:766112/1‑151 (MQ=255)
                                                                                                                                                      cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAATTCCCCCCCCAAGCAACAATTATCTTTAATTCCTTTCTTGTTTCTTACATGAttt  >  1:1693790/1‑151 (MQ=21)
                                                                                                                                                      cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGAttt  >  4:814790/1‑151 (MQ=255)
                                                                                                                                                      cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGGCCCCCCCCGCGCCCCCAGTATCTTTTCTTACGTTATTGTTTGTTTCTTGGttt  >  8:764055/1‑151 (MQ=18)
                                                                                                                                                      |                                                                                                                                                      
GGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906687‑3906987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑