Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA26.0% 434.0 / 21.5 297intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (130/52);  new base A (63/1);  total (241/56)
Fisher's exact test for biased strand distribution p-value = 6.25e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

TACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGGA  >  DH1/4121978‑4122273
                                                                                                                                                  |                                                                                                                                                     
aaacAACGGAGCTATTCGCTCTTGGGTGGTGTGTGCATTACAGCACTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTg                                                                                                                                                   <  6:572633/148‑1 (MQ=17)
    aaCTGTGCTATTCCCGTCTGGGTGGTCTGTTCATTACAGTTCTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgcagc                                                                                                                                               <  6:281371/151‑1 (MQ=17)
        gAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGCGACGCGCACTCGCGACCCCGACCTTGGCAAGGTCGTGGTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTTTGCAGCACtt                                                                                                                                           >  7:1742841/1‑151 (MQ=255)
         aGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCCACCCCCAACTTGGCAAGGTAGTGCTCTACCAACAGAGATAATCCCAGATCGGTCGTCTATGCATTgcaggaaatt                                                                                                                                          >  8:1926621/1‑145 (MQ=14)
          ccTATCCCCGCTTGGGCGCTCTGTTCCTTAAAGAACTATAACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACtttt                                                                                                                                         <  8:907208/150‑1 (MQ=14)
           cTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTGTGGAGGACtttt                                                                                                                                         >  4:945765/1‑150 (MQ=255)
             attcgcgagtGGGGGGTGTGTGCCTTACATAACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTc                                                                                                                                        <  3:38954/140‑1 (MQ=17)
             aTTCCCGCTGGGGGGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTc                                                                                                                                        <  3:973724/149‑1 (MQ=255)
               tcgcGCTTGGGTGGTCTGTTTATTTCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaaa                                                                                                                                     <  8:1207820/147‑1 (MQ=255)
                 ccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTGGTGGTCTTTTATTTTGAGCAATTTTCAAAttt                                                                                                                                  >  6:1334343/1‑151 (MQ=14)
                  cGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTATGTGCTTTTAAGCAATTTTCAAAtttt                                                                                                                                 >  7:1270089/1‑151 (MQ=255)
                   gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGAGGTGGAGCACTTTTCAAAttttt                                                                                                                                >  6:1898770/1‑150 (MQ=255)
                     ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTGGTCTGTTCTTTTGAGAAATTTTCAAAtttttgc                                                                                                                              >  3:681014/1‑148 (MQ=17)
                      tggttcttgtgtaacTTTCAGCACTATAACAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGa                                                                                                                              <  5:628165/136‑1 (MQ=14)
                      gggggggTGTGTCCCTTACATGACTTTCACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                               <  5:1615504/148‑1 (MQ=17)
                       gggTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTGTGCAGCACTTTTCAAATTTTGGAGc                                                                                                                            >  8:1117585/1‑151 (MQ=255)
                          tggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTATGTGATGTGCAGGACTTTTCAAATTTTGGCGCggg                                                                                                                         >  4:376571/1‑151 (MQ=17)
                          tggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTCTTTTTTTTTTAGGAATTTTCAAATTTTAGATCggg                                                                                                                         >  3:238098/1‑151 (MQ=17)
                          ggctgtgtgCATTATATCCCTATCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCggg                                                                                                                         <  7:272262/146‑1 (MQ=14)
                           ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTGTTTGGAGCACTTTTCAAATTTTGGAGCggg                                                                                                                         >  6:184554/1‑150 (MQ=255)
                            gtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTGTGGGGTGGCGCACTTTTCGAATTTAGGAGCcggt                                                                                                                        >  7:563631/1‑149 (MQ=17)
                            gtCTGGCCCTTACATTTCTTTCATAATTTGGACCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGGCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGaa                                                                                                                       <  5:146939/151‑1 (MQ=14)
                             tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTGTGGTTTTCAGAAATTTTCAAATTTTTGCGCGGGaa                                                                                                                       >  2:553018/1‑150 (MQ=17)
                             tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTTTTATATGGAGCAATTTTCAAATTTTGGAGCGGGaa                                                                                                                       >  2:586326/1‑150 (MQ=18)
                              cTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTGTGTGGAGGAATTTTCTAATTTTGGAGTGGGaacc                                                                                                                     >  1:914545/1‑149 (MQ=17)
                              cTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCGGTGGAGCACTTTTCAAATTTTGGAGCGGGAAAc                                                                                                                     >  3:846992/1‑151 (MQ=255)
                               tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTCGTCTGTTATTTGGAGAACTTTTCGAATTTGGGAGGGGTAAACg                                                                                                                    >  5:1419323/1‑151 (MQ=17)
                                gttcatttcatgtatttCAAATTTTGGAGGGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGaaa                                                                                                                      <  8:1527399/134‑1 (MQ=14)
                                gtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGACGTATGTGGTGTGGAGCACTTTTCAAATTTTGGAGGGGGAACCgc                                                                                                                   >  5:1734852/1‑150 (MQ=17)
                                cgccagtACAGGACCTTCAAAATTTCGAGCCGGAAACGAGACTCGAACTCGCGACCCCGACCATGGCAAGGGCGAGCTCTACCAACTGAGCTATACCCGCTTGGGTGGGCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACg                                                                                                                    <  5:1887568/144‑1 (MQ=14)
                                   ccTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGAc                                                                                                                >  8:168267/1‑151 (MQ=255)
                                   ccTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGGTTTGGAGCACTTTTCAAATTTTTGAGCCGGAAACgcgcc                                                                                                                >  3:1472952/1‑147 (MQ=17)
                                   ccTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTCTGTTGTTTGAAGCACTTTTCAAATTTTGGAGCGGGAACCGCGAc                                                                                                                >  1:636269/1‑151 (MQ=17)
                                   ccTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCGTCTGTTGTGTGAAGCAATTTTCAAATTTAGGAGAGGGAAACgagcc                                                                                                                >  6:1957868/1‑149 (MQ=17)
                                    cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTATGTTATTTTAAGAAATTTTCAAATTTTTGAGAGGTAAACGAGAcg                                                                                                               >  2:855454/1‑150 (MQ=17)
                                     ttACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTCGTCTGTTATATTAAGCACTTTTCAAATTTTGGAGAGGGAACCGAGACTc                                                                                                              >  6:406329/1‑151 (MQ=17)
                                     ttACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTGTTATTTGAAGAACTTTTCAAATTTTGGAGAGGGAAACGAGACTc                                                                                                              >  2:1321983/1‑151 (MQ=17)
                                            aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCTTGGTCTGTGAGATGAAGTAATTTTCGAATTTTCGCGTCGGAAACgacccgcgaaaac                                                                                                        >  1:1023652/1‑139 (MQ=11)
                                               ttCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTGTGTTGTGGAGGACTTTTCAAATTTTGGAGAGGGAACCGAGACTGGATCTcgcg                                                                                                     >  1:1507070/1‑150 (MQ=14)
                                                 cAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTAGCGAcc                                                                                                  >  4:1838061/1‑151 (MQ=255)
                                                   aaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGGGTTGGCGGACTTTTCGAATTTTGGAGAGGGTAACGCGCGTCGATCTAGCGAccac                                                                                                >  7:1623666/1‑149 (MQ=14)
                                                    aTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTGGTATGTTATATTAAGGACTTTTCAAATTTATGAGACGTAAACGAGACTCCACCTCGACAccc                                                                                                 >  8:691228/1‑149 (MQ=11)
                                                      tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTACGAGGTCTTTTATATGAAGAAATTTTCAAATTTATGAGACGGAAACGAGACTCGATCTAACGAcaaagc                                                                                              >  4:229020/1‑145 (MQ=14)
                                                      tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTATTTTGTATGACGCACTTTTCACATTTATGAGGGGGTACCGGGACGCGaggtggcgagaactcc                                                                                             >  6:1198163/1‑136 (MQ=2)
                                                        tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTATTTTTTGTGGAGCACTTTTCAAATTTTTGAGCGGGAAACGAGTCTCGAAATCCCGAcaact                                                                                               >  7:954025/1‑143 (MQ=14)
                                                             cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTCTGTGCTATGAAGAACTTTTCAAATTTTGGAGAGGGAAACGCAACTCGAAATCGCGACCCCGACCtagcc                                                                                       >  6:74760/1‑146 (MQ=14)
                                                               ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTGGTCTGTGATATGCAGAAATTTTCAAATTTTTGAGCGGGAAACGAGACTCGAAATAGCGACCACGAACtacgca                                                                                      >  8:62684/1‑144 (MQ=14)
                                                                 aaaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGt                                                                                  >  7:1922372/1‑151 (MQ=38)
                                                                 aaaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGGAGAACTTTTCAAATTTTTGAGAGGTAAAGGAGACTCGTAATAGCGACCACGAGATAGGCAAGGt                                                                                  >  4:730079/1‑151 (MQ=14)
                                                                    cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGt                                                                               >  5:376028/1‑151 (MQ=255)
                                                                      cgagtcgacccctcgACCCCTACATTGGAAAGGTCGTTATCTAAAAACTTCGCTATTCCCTTTTGGGGGGGGTGTTGTTTTAAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc                                                                             <  8:1952892/138‑1 (MQ=2)
                                                                        aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTATGTGATGTGAAGCGCTGTGCAAATTTTGGAGCGCGAACCGACACTGGACCTAGCCACACAGCCCTTGGCAAAGTAGTGctc                                                                           >  3:1694312/1‑151 (MQ=2)
                                                                         cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                          >  3:640011/1‑151 (MQ=38)
                                                                           cGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTATGTTGTTTGGAGGAATTTTCCAATTTTGGAGAGGTAACCGGGCGTCGTACTAGCGACCCCGCGCTTGGCAAGCTGGTGCGCTa                                                                         >  3:1569744/1‑150 (MQ=9)
                                                                           cGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                        >  5:1231852/1‑151 (MQ=38)
                                                                            gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGCCTCGAACTCGCGACCCCGACCTTGGGAAGGTCGTGCGCTAc                                                                        >  5:1832499/1‑150 (MQ=255)
                                                                               cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGAAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAc                                                                    >  2:523588/1‑151 (MQ=255)
                                                                               cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTTTGCAGGACTTTTCAAATTTTGGAGCGGGAACCGCGCCTCGAACTCGCGACCCCGCGCTTGGCAAGGTCGTGCTCTACCagc                                                                    >  1:31072/1‑149 (MQ=14)
                                                                                  ccgACCCCGACCTTTGCAAGGTCTTGCTCTACCAACTCAGCTATTCCCGCTTGGGTTGTATTTGCTTTGCATCAATTTTCAAATTTTGTAGCGGGAAACGAGACTCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGa                                                                 <  8:108166/150‑1 (MQ=14)
                                                                                    gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGCTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCGTGCGCAACCAACTGAGc                                                               >  7:374820/1‑151 (MQ=255)
                                                                                     aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt                                                              >  3:597444/1‑151 (MQ=38)
                                                                                     aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGAAGAACTTTTCAAATTTTGGAGACGGAAACGACACTCCAAAACGCGACAAAGAACTGGGCAAGCTCGTCCTCAACCAACTg                                                                  >  6:1671126/1‑147 (MQ=14)
                                                                                     aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTCGTCTGTGATTTGCAGAAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCTACGTTCCAAAGCACCTGCTCTACAAACTGAGCt                                                              >  4:1411250/1‑151 (MQ=14)
                                                                                      ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTAGGTCGTCTGTTATTTGAAGCACTTTTCAAATTTTGGAGAGGTAAAGGAGACTCCAACTAGCGACCCCTCGATTCGCAAGGTCGTGCTCTAACAACTGAgat                                                              >  6:1477921/1‑148 (MQ=14)
                                                                                        ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTATGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAAGTCGCGACCCCGACCTGGGCAAGCTCCAGCGCTACCAACTGAGCTAtt                                                           >  1:1954762/1‑151 (MQ=14)
                                                                                          gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTCTTTTAGTTGTAGAAATTTTCACATTTTTGAGAGGGAAAGGCGCCGCGAACTCGCGACAACGACCTGGGACAGCACCTGCTCTACCAACTTAGCTATTcc                                                         >  3:288147/1‑151 (MQ=2)
                                                                                             cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGAAGCAATTTTCAAATTTTTGAGAGGGAAACGAGACTCGAAAAAGCGACCCCGACATTGCAAAGGACGTGCTCTAAAAACTGAGATATTca                                                         >  8:412855/1‑147 (MQ=14)
                                                                                               tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACAAACTGAGGTATTCCCGCGt                                                    >  7:1206872/1‑151 (MQ=255)
                                                                                                   aaGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCCGCACTTTTCAAATTTTGGCGCGGGAACCGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCGTGCGCTACCAAGTGAGCTATTCCCGCGtact                                                 >  5:1181072/1‑150 (MQ=14)
                                                                                                    aGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTATGTTATTTGGAGGAATTTTCAAATTTTTGAGCGGTAAACGAGACTCGAACTCGCGACCACGAGCTTCGCAAGGTCGGGCTCTACAAACTGAGCTATTCCAGCGtacgac                                               >  3:1362070/1‑151 (MQ=14)
                                                                                                     ggTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGA‑CCCGACCTTGGCAAGGTCGTGCTCTACAAACTGAGCTATTCCCGCGTACTACtt                                             >  7:1090871/1‑151 (MQ=255)
                                                                                                      ttcttGCTCTACCTACTTTGCTATTCCGGCTTGGGTGGTTTGTGCTTTTTATTTGTTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTactact                                              <  4:641549/146‑1 (MQ=14)
                                                                                                      gTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCACGACCTTCGCAAGGTCGTGCTCTACCAACTGAGATATTCCCGCGTACTACtt                                             >  7:622773/1‑151 (MQ=255)
                                                                                                        cGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTGTTATTTGAAGAAATTTTCAAATTTAGGAGAGGGAACCGAGACTCGAACTAACGACCACTACCTAGCCAAGCTCCAGCACTAACAACTGAGATATTCCAGATTACTACTTa                                            >  4:1551242/1‑150 (MQ=9)
                                                                                                         gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCTCCCTAGGCAAGGTCGAGCGCTACCAACTGAGCTATTCCCGCGTACTACTGAAt                                          >  2:294466/1‑151 (MQ=14)
                                                                                                         gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAt                                          >  5:234032/1‑151 (MQ=255)
                                                                                                         gTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTATGTGCTTTTCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACACGAACTCGCGACCCCGACCTTGGAAAGGTCGTGCTCTACCAACTGAGATATTCCCGCGTAGTACTTAAt                                          >  2:646790/1‑151 (MQ=14)
                                                                                                             tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                      >  1:1629394/1‑151 (MQ=255)
                                                                                                             tctACCAACTCCGCTATTCCCGCTTGGGTGGGCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAtttt                                       <  3:1138236/150‑1 (MQ=255)
                                                                                                                 cccaccgccccctgcccGCGTGGGGGGGCCGGGCCCTGCATCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                  <  3:337684/137‑1 (MQ=17)
                                                                                                                 ccAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                  >  3:152435/1‑151 (MQ=255)
                                                                                                                 ccAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTCTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                  >  1:553417/1‑151 (MQ=255)
                                                                                                                  cAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCTCGTACTACTTAATTTTGCttt                                  >  2:1409136/1‑150 (MQ=255)
                                                                                                                  cAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTc                                 >  7:854146/1‑151 (MQ=255)
                                                                                                                     cTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                               >  2:1048102/1‑150 (MQ=255)
                                                                                                                      tGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTa                              >  2:933215/1‑150 (MQ=255)
                                                                                                                         gCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAttt                          >  8:1742816/1‑151 (MQ=255)
                                                                                                                          cTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCAC‑TTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTACTTCTTAAtttatgttaagaaatttt                         >  2:1776434/1‑135 (MQ=11)
                                                                                                                           tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                        >  4:1704904/1‑151 (MQ=255)
                                                                                                                              tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                      >  3:1757916/1‑150 (MQ=255)
                                                                                                                                  gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtttg                 >  7:1536467/1‑150 (MQ=255)
                                                                                                                                  gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTc                 >  5:794801/1‑151 (MQ=255)
                                                                                                                                     tGGGTGGTCTGTGCCTTACAGCAC‑TTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCTTGTTATTTTTTGTTTCGTAATTTTCAAATTTTTGcg             >  5:176958/1‑151 (MQ=11)
                                                                                                                                      gggTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcg             >  5:52272/1‑151 (MQ=255)
                                                                                                                                           gtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt        >  7:188955/1‑151 (MQ=255)
                                                                                                                                              tgtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACg     >  6:1406744/1‑151 (MQ=255)
                                                                                                                                              tgtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACg     >  8:325445/1‑151 (MQ=255)
                                                                                                                                              ggggCTTGGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACg     <  7:689652/148‑1 (MQ=255)
                                                                                                                                                tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTCCCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg   >  5:950464/1‑151 (MQ=255)
                                                                                                                                                tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg   >  8:52754/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGGA  >  DH1/4121978‑4122273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑